TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Živković, Ljiljana
AU  - Živković, Natalija
AU  - Ilicic, Renata
AU  - Stanisavljević, Rade
AU  - Stanković, Slaviša
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1350
AB  - Fire blight disease, caused by plant pathogenic bacteriaErwinia amylovorarepresents a global threat for pome fruit industry, affecting yield and causing serious economic damage. During six year period (2011-2016), a total of 45 isolates typical forE. amylovorawere isolated from four different host plants (apple, pear, quince and medlar) in 21 localities within four Serbian regions (Southeast, Central, West, and North). Isolates were primarily identified using biochemical and serological tests, polymerase chain reaction (PCR) with species-specific primer pair (A/B) and their pathogenicity. Polyclonal antibodies used in DAS ELISA and IF were found to be specific and sensitive enough for rapidE. amylovoradetection. In virulence test performed on immature pear fruitlets, higher variability (CV) was found between regions than among hosts from which isolates originated. Slighter virulence of isolates originated from North region of Serbia (Vojvodina) was found in comparison to the isolates from Central Serbia, which were the most variable. Principal components analysis (PCA) segregated quince as a host among the most prevalent isolates in group of highly virulent, but failed to identify difference related to geographic origin of Serbian isolates. The same results enabled multivariate statistical analysis. Different DNA fingerprinting techniques such as repetitive element sequence based PCR (rep-) with BOX, ERIC, REP, GTG(5)and SERE primers, then Random Amplified Polymorphic DNA (RAPD) with M13-PCR primer revealed genetic homogeneity among all SerbianE. amylovoraisolates. ERIC, SERE and M13 primers generated the most complex and visually perceptible patters. Restriction fragment length polymorphism analysis (RFLP) ofgyrAandrecAgenes, digested with three restriction endonucleasesBstUI,BgIII andBsmI andrpoSgene digested withBstUI gave the unuque, characteristic patterns for all Serbian isolates.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates
EP  - 872
IS  - 4
SP  - 857
VL  - 157
DO  - 10.1007/s10658-020-02046-1
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Živković, Ljiljana and Živković, Natalija and Ilicic, Renata and Stanisavljević, Rade and Stanković, Slaviša",
year = "2020",
abstract = "Fire blight disease, caused by plant pathogenic bacteriaErwinia amylovorarepresents a global threat for pome fruit industry, affecting yield and causing serious economic damage. During six year period (2011-2016), a total of 45 isolates typical forE. amylovorawere isolated from four different host plants (apple, pear, quince and medlar) in 21 localities within four Serbian regions (Southeast, Central, West, and North). Isolates were primarily identified using biochemical and serological tests, polymerase chain reaction (PCR) with species-specific primer pair (A/B) and their pathogenicity. Polyclonal antibodies used in DAS ELISA and IF were found to be specific and sensitive enough for rapidE. amylovoradetection. In virulence test performed on immature pear fruitlets, higher variability (CV) was found between regions than among hosts from which isolates originated. Slighter virulence of isolates originated from North region of Serbia (Vojvodina) was found in comparison to the isolates from Central Serbia, which were the most variable. Principal components analysis (PCA) segregated quince as a host among the most prevalent isolates in group of highly virulent, but failed to identify difference related to geographic origin of Serbian isolates. The same results enabled multivariate statistical analysis. Different DNA fingerprinting techniques such as repetitive element sequence based PCR (rep-) with BOX, ERIC, REP, GTG(5)and SERE primers, then Random Amplified Polymorphic DNA (RAPD) with M13-PCR primer revealed genetic homogeneity among all SerbianE. amylovoraisolates. ERIC, SERE and M13 primers generated the most complex and visually perceptible patters. Restriction fragment length polymorphism analysis (RFLP) ofgyrAandrecAgenes, digested with three restriction endonucleasesBstUI,BgIII andBsmI andrpoSgene digested withBstUI gave the unuque, characteristic patterns for all Serbian isolates.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates",
pages = "872-857",
number = "4",
volume = "157",
doi = "10.1007/s10658-020-02046-1"
}

Popović, T., Jelušić, A., Živković, L., Živković, N., Ilicic, R., Stanisavljević, R.,& Stanković, S.. (2020). Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates. in European Journal of Plant Pathology
Springer, Dordrecht., 157(4), 857-872.
https://doi.org/10.1007/s10658-020-02046-1

Popović T, Jelušić A, Živković L, Živković N, Ilicic R, Stanisavljević R, Stanković S. Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates. in European Journal of Plant Pathology. 2020;157(4):857-872.
doi:10.1007/s10658-020-02046-1 .

Popović, Tatjana, Jelušić, Aleksandra, Živković, Ljiljana, Živković, Natalija, Ilicic, Renata, Stanisavljević, Rade, Stanković, Slaviša, "Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates" in European Journal of Plant Pathology, 157, no. 4 (2020):857-872,
https://doi.org/10.1007/s10658-020-02046-1 . .

TY  - CONF
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Živković, Natalija
AU  - Dimkić, Ivica
AU  - Nikolić, Ivan
AU  - Stanković, Slaviša
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1929
AB  - Recent studies have been made to investigate some molecular features of plant pathogenic 
bacteria Pseudomonas syringae pv. coriandricola. This pathogen causes bacterial leaf spot 
disease on some members of family Apiaceae, such as coriander, carrot, parsnip and parsley. 
Initial symptoms are small water-soaked lesions on foliage, which then develop into spots 
varying in shades, tan, brown or black. The spots are usually limited by leaf veins having an 
angular appearance, and visible from both sides of leaves. Under favorable conditions of high 
humidity, spots may spread and cause foliage blighting. P. s. pv. coriandricola strains used in this 
study originate from carrot, parsley and parsnip. Repetitive element PCR fingerprinting (rep PCR), using REP, BOX, ERIC, (GTG)5 and SERE primers, and randomly amplified polymorphic 
DNA-polymerase chain reaction (RAPD-PCR) using M13 primer were used to determinate 
genetic polymorphism between tested strains. Further molecular features were observed on the 
basis of a multilocus sequence analysis (MLSA) by sequencing key genes for Pseudomonas 
syringae (gapA, gltA, gyrB, rpoD). DNA profiling revealed identical banding patterns for all 
tested strains. The sequence and phylogenetic analysis of three MLSA genes (gapA, gyrB, rpoD) 
showed homology with pathotype strains P. s. pv. coriandricola deposited in PAMDB 
(http://genome.ppws.vt.edu/cgi-bin/MLST/home.pl). Sequencing of gltA gene determined the 
isolates above the species level (P. syringae). Our results indicate that gapA, gyrB and rpoD 
genes are more discriminatory then gltA and their usage is therefore suggested for further 
genotypic studies of this pathogen.
PB  - INTERNATIONAL Scientific Agricultural Symposium "Agrosym 2017"
C3  - VIII International Scientific Agriculture Symposium “AGROSYM 2017”
T1  - MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV.  CORIANDRICOLA ORIGINATED FROM CARROT, PARSLEY AND PARSNIP
SP  - 549
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1929
ER  - 

@conference{
author = "Popović, Tatjana and Jelušić, Aleksandra and Marković, Sanja and Živković, Natalija and Dimkić, Ivica and Nikolić, Ivan and Stanković, Slaviša",
year = "2017",
abstract = "Recent studies have been made to investigate some molecular features of plant pathogenic 
bacteria Pseudomonas syringae pv. coriandricola. This pathogen causes bacterial leaf spot 
disease on some members of family Apiaceae, such as coriander, carrot, parsnip and parsley. 
Initial symptoms are small water-soaked lesions on foliage, which then develop into spots 
varying in shades, tan, brown or black. The spots are usually limited by leaf veins having an 
angular appearance, and visible from both sides of leaves. Under favorable conditions of high 
humidity, spots may spread and cause foliage blighting. P. s. pv. coriandricola strains used in this 
study originate from carrot, parsley and parsnip. Repetitive element PCR fingerprinting (rep PCR), using REP, BOX, ERIC, (GTG)5 and SERE primers, and randomly amplified polymorphic 
DNA-polymerase chain reaction (RAPD-PCR) using M13 primer were used to determinate 
genetic polymorphism between tested strains. Further molecular features were observed on the 
basis of a multilocus sequence analysis (MLSA) by sequencing key genes for Pseudomonas 
syringae (gapA, gltA, gyrB, rpoD). DNA profiling revealed identical banding patterns for all 
tested strains. The sequence and phylogenetic analysis of three MLSA genes (gapA, gyrB, rpoD) 
showed homology with pathotype strains P. s. pv. coriandricola deposited in PAMDB 
(http://genome.ppws.vt.edu/cgi-bin/MLST/home.pl). Sequencing of gltA gene determined the 
isolates above the species level (P. syringae). Our results indicate that gapA, gyrB and rpoD 
genes are more discriminatory then gltA and their usage is therefore suggested for further 
genotypic studies of this pathogen.",
publisher = "INTERNATIONAL Scientific Agricultural Symposium "Agrosym 2017"",
journal = "VIII International Scientific Agriculture Symposium “AGROSYM 2017”",
title = "MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV.  CORIANDRICOLA ORIGINATED FROM CARROT, PARSLEY AND PARSNIP",
pages = "549",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1929"
}

Popović, T., Jelušić, A., Marković, S., Živković, N., Dimkić, I., Nikolić, I.,& Stanković, S.. (2017). MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV.  CORIANDRICOLA ORIGINATED FROM CARROT, PARSLEY AND PARSNIP. in VIII International Scientific Agriculture Symposium “AGROSYM 2017”
INTERNATIONAL Scientific Agricultural Symposium "Agrosym 2017"., 549.
https://hdl.handle.net/21.15107/rcub_rimsi_1929

Popović T, Jelušić A, Marković S, Živković N, Dimkić I, Nikolić I, Stanković S. MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV.  CORIANDRICOLA ORIGINATED FROM CARROT, PARSLEY AND PARSNIP. in VIII International Scientific Agriculture Symposium “AGROSYM 2017”. 2017;:549.
https://hdl.handle.net/21.15107/rcub_rimsi_1929 .

Popović, Tatjana, Jelušić, Aleksandra, Marković, Sanja, Živković, Natalija, Dimkić, Ivica, Nikolić, Ivan, Stanković, Slaviša, "MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE PV.  CORIANDRICOLA ORIGINATED FROM CARROT, PARSLEY AND PARSNIP" in VIII International Scientific Agriculture Symposium “AGROSYM 2017” (2017):549,
https://hdl.handle.net/21.15107/rcub_rimsi_1929 .