TY  - JOUR
AU  - Bosnic, Dragana
AU  - Bosnić, Predrag
AU  - Nikolic, Dragana
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1220
AB  - Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves
IS  - 12
VL  - 8
DO  - 10.3390/plants8120554
ER  - 

@article{
author = "Bosnic, Dragana and Bosnić, Predrag and Nikolic, Dragana and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2019",
abstract = "Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves",
number = "12",
volume = "8",
doi = "10.3390/plants8120554"
}

Bosnic, D., Bosnić, P., Nikolic, D., Nikolic, M.,& Samardžić, J. T.. (2019). Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel
MDPI, Basel., 8(12).
https://doi.org/10.3390/plants8120554

Bosnic D, Bosnić P, Nikolic D, Nikolic M, Samardžić JT. Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel. 2019;8(12).
doi:10.3390/plants8120554 .

Bosnic, Dragana, Bosnić, Predrag, Nikolic, Dragana, Nikolic, Miroslav, Samardžić, Jelena T., "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves" in Plants-Basel, 8, no. 12 (2019),
https://doi.org/10.3390/plants8120554 . .

TY  - JOUR
AU  - Nikolic, Dragana B.
AU  - Nesic, Sofija
AU  - Bosnic, Dragana
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1284
AB  - The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
VL  - 10
DO  - 10.3389/fpls.2019.00416
ER  - 

@article{
author = "Nikolic, Dragana B. and Nesic, Sofija and Bosnic, Dragana and Kostić Kravljanac, Ljiljana and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2019",
abstract = "The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution",
volume = "10",
doi = "10.3389/fpls.2019.00416"
}

Nikolic, D. B., Nesic, S., Bosnic, D., Kostić Kravljanac, L., Nikolic, M.,& Samardžić, J. T.. (2019). Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fpls.2019.00416

Nikolic DB, Nesic S, Bosnic D, Kostić Kravljanac L, Nikolic M, Samardžić JT. Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science. 2019;10.
doi:10.3389/fpls.2019.00416 .

Nikolic, Dragana B., Nesic, Sofija, Bosnic, Dragana, Kostić Kravljanac, Ljiljana, Nikolic, Miroslav, Samardžić, Jelena T., "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution" in Frontiers in Plant Science, 10 (2019),
https://doi.org/10.3389/fpls.2019.00416 . .

TY  - JOUR
AU  - Bosnic, Dragana
AU  - Nikolic, Dragana
AU  - Timotijević, Gordana
AU  - Pavlović, Jelena
AU  - Vaculik, Marek
AU  - Samardžić, Jelena T.
AU  - Nikolic, Miroslav
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1212
AB  - Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity
EP  - 641
IS  - 1-2
SP  - 629
VL  - 441
DO  - 10.1007/s11104-019-04151-5
ER  - 

@article{
author = "Bosnic, Dragana and Nikolic, Dragana and Timotijević, Gordana and Pavlović, Jelena and Vaculik, Marek and Samardžić, Jelena T. and Nikolic, Miroslav",
year = "2019",
abstract = "Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity",
pages = "641-629",
number = "1-2",
volume = "441",
doi = "10.1007/s11104-019-04151-5"
}

Bosnic, D., Nikolic, D., Timotijević, G., Pavlović, J., Vaculik, M., Samardžić, J. T.,& Nikolic, M.. (2019). Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil
Springer, Dordrecht., 441(1-2), 629-641.
https://doi.org/10.1007/s11104-019-04151-5

Bosnic D, Nikolic D, Timotijević G, Pavlović J, Vaculik M, Samardžić JT, Nikolic M. Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil. 2019;441(1-2):629-641.
doi:10.1007/s11104-019-04151-5 .

Bosnic, Dragana, Nikolic, Dragana, Timotijević, Gordana, Pavlović, Jelena, Vaculik, Marek, Samardžić, Jelena T., Nikolic, Miroslav, "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity" in Plant and Soil, 441, no. 1-2 (2019):629-641,
https://doi.org/10.1007/s11104-019-04151-5 . .

TY  - CONF
AU  - Nikolic, Dragana B.
AU  - Nesic, Sofija
AU  - Bosnic, Dragana
AU  - Kostić, Ljiljana
AU  - Nikolic, Miroslav
AU  - Samardzic, Jelena
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2478
AB  - The beneficial effects of silicon (Si) on various abiotic and biotic stresses in plants are well established; however, molecular
mechanisms are not completely understood. An ameliorative effect of Si on iron (Fe) deficiency stress has only been shown
on plants which use the reduction-based strategy (Strategy I) for Fe acquisition. The aim of our study was to investigate
influence of Si on Fe deficiency stress alleviation in a cereal plant which uses the chelation-based strategy (Strategy II) for
Fe acquisition, and barley was chosen as a representative.
Si successfully ameliorated Fe deficiency in barley, attenuating chlorosis and biomass loss of the youngest leaves, as well
as ROS accumulation, accompanied with the recovered activities of antioxidative enzymes, ascorbate peroxidase and
catalase. Si increased Fe content in the youngest leaves of Fe deprived plants, as well as Fe concentration in the watersoluble
(w-s) fraction. On the other hand, w-s concentration and total content of optimally supplied microelements, Mn and
Zn, were decreased in Si supplied plants. The expression of Strategy II genes was modulated under the influence of Si. An
expeditious increase in the gene expression was detected in Fe deficient roots. Moreover, a dramatic Si-promoted
upregulation of some of the investigated genes was detected in leaves.
Fe deficiency in plants due to low Fe availability in soils has a considerable impact on both yield and nutritional value of
crops. New findings presented in our study may support development of strategies to overcome this substantial agricultural
problem.
PB  - University of Copenhagen, Denmark
C3  - Plant Biology Europe 2018 Conference Abstract Book
T1  - The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2478
ER  - 

@conference{
author = "Nikolic, Dragana B. and Nesic, Sofija and Bosnic, Dragana and Kostić, Ljiljana and Nikolic, Miroslav and Samardzic, Jelena",
year = "2018",
abstract = "The beneficial effects of silicon (Si) on various abiotic and biotic stresses in plants are well established; however, molecular
mechanisms are not completely understood. An ameliorative effect of Si on iron (Fe) deficiency stress has only been shown
on plants which use the reduction-based strategy (Strategy I) for Fe acquisition. The aim of our study was to investigate
influence of Si on Fe deficiency stress alleviation in a cereal plant which uses the chelation-based strategy (Strategy II) for
Fe acquisition, and barley was chosen as a representative.
Si successfully ameliorated Fe deficiency in barley, attenuating chlorosis and biomass loss of the youngest leaves, as well
as ROS accumulation, accompanied with the recovered activities of antioxidative enzymes, ascorbate peroxidase and
catalase. Si increased Fe content in the youngest leaves of Fe deprived plants, as well as Fe concentration in the watersoluble
(w-s) fraction. On the other hand, w-s concentration and total content of optimally supplied microelements, Mn and
Zn, were decreased in Si supplied plants. The expression of Strategy II genes was modulated under the influence of Si. An
expeditious increase in the gene expression was detected in Fe deficient roots. Moreover, a dramatic Si-promoted
upregulation of some of the investigated genes was detected in leaves.
Fe deficiency in plants due to low Fe availability in soils has a considerable impact on both yield and nutritional value of
crops. New findings presented in our study may support development of strategies to overcome this substantial agricultural
problem.",
publisher = "University of Copenhagen, Denmark",
journal = "Plant Biology Europe 2018 Conference Abstract Book",
title = "The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2478"
}

Nikolic, D. B., Nesic, S., Bosnic, D., Kostić, L., Nikolic, M.,& Samardzic, J.. (2018). The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution. in Plant Biology Europe 2018 Conference Abstract Book
University of Copenhagen, Denmark..
https://hdl.handle.net/21.15107/rcub_rimsi_2478

Nikolic DB, Nesic S, Bosnic D, Kostić L, Nikolic M, Samardzic J. The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution. in Plant Biology Europe 2018 Conference Abstract Book. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2478 .

Nikolic, Dragana B., Nesic, Sofija, Bosnic, Dragana, Kostić, Ljiljana, Nikolic, Miroslav, Samardzic, Jelena, "The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution" in Plant Biology Europe 2018 Conference Abstract Book (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2478 .