TY  - CONF
AU  - Milovanović, Jelena
AU  - Ilić, Bojan
AU  - Makarov, Slobodan
AU  - Đorđević Aleksić, Jelena
AU  - Stevanović, Aleksandra
AU  - Malešević, Anastasija
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2178
AB  - INTRODUCTION:
Different orders within the class Diplopoda possess a variety of chemical compounds in their defense secretions: quinones, phenolics, alcohols, aldehydes, ketones, esters, alkaloids, cyanogenic compounds. Defensive secretions of species from the order Julida are regarded as the most complex within Diplopoda, and they are blends of several classes of chemical com- pounds: quinones, esters and ketones. Numerous biological activities of these secretions have been reported: antimicrobial, antioxidative, antineurodegenerative, cytotoxic and embryotoxic on zebrafish. Besides zebrafish embryos, Artemia salina is one of the common model organisms in toxicity assessment which has not been used for screening of toxicity of millipedes’ defensive secretions.
OBJECTIVES:
The main goal of this study was to examine the toxic effects of defensive secretions of two species from the order Julida [Megaphyllum bosniense (MBO) and M. unilineatum (MUN)] using Artemia salina (ARC test).
METHOD / DESIGN:
Adult individuals of M. bosniense were collected during April and May of 2021 on Mt. Avala, near Belgrade, while adults of M. unilineatum were collected during the same period in the Krnjača, suburb of Belgrade. After the capture, millipedes were kept in plastic boxes containing ground cover from the collecting site. The boxes were regularly sprayed with water to maintain high humidity. Due to the fact that the sample was female-biased, defensive secretions of female specimens were used for further analyses. Excretion of defensive secretions was elicited from glands of mentioned species via mechanical stress in closed glass vials. Secretions collected from both species were dissolved in 10 ml of hexane, concentrated under reduced pressure in a rotary evaporator (Rotavapor R-210, Buchi) at 40°C to a dry residue, and redissolved in 50% dimethylsulphoxide (DMSO). The stock concentration of extracts used in ARC test was 20 mg/mL. Before treatments, eggs of A. salina were incubated for 72h with constant lighting and aeration. For the purposes of the experiment, stage II and III larvae were used (separated by phototaxis in 300 ml of seawater). In a plate with 24 wells, 900 μl of seawater with larvae (10-15 per well) was placed and then 100 μl of tested extracts (range of concentrations 0,1 mg/mL - 0,003125 mg/mL) was added. Potassium dichromate (K2Cr2O7) was used as a positive control and DMSO was used as solvent control. The total number of individuals per well was counted after 24h and 48h, as well as the number of living and dead individuals. These data were used for esti- mation of survival rate and determination of LC50 value. The experiment was done in triplicate.
RESULTS:
Our results show that secretions from both species exhibit a toxic effect on the survival of the chosen model organism, with the MBO extract showing weaker activity in comparison with MUN extract. The LC50 value after 24h was about the same for both species (LC50=73,23 μg/mL for MBO and LC50=68,56 μg/mL for MUN). The LC50 value for MBO after 48h was 47,18 μg/ mL, while LC50 value in the same period for MUN was 29,12 μg/mL. Positive control (LC50= 13,5 μg/mL) showed three times stronger effects in relation to MBO and twice as strong when compared to MUN extract. It has also been shown that the num- ber of surviving individuals decreases with increasing concentration of tested extracts and the increasing incubation time.
CONCLUSIONS:
The defense secretions of both tested millipede species show toxic effects in the ARC test. It is shown that MBO extract has a weaker toxic effect than the MUN extract. This result can be linked with the fact that esters of long-chain fatty acids are dominant compounds in MBO, while MUN is almost exclusively benzoquinone-based. Esters detected in MBO are generally regarded as low-toxic compounds, but with the potential to interact with compounds from other chemical classes. However, as MBO achieved toxic effects and many esters that are detected in MBO are new natural products and their biological potential is unknown, further extensive studies are needed to determine their toxicological potential.
PB  - The International Bioscience Conference (2021; Novi Sad)
C3  - International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021
T1  - Acute toxicity assessment of defense secretions of Megaphyllum bosniense (Verhoeff, 1897) and M. unilineatum (C. L. Koch, 1838) (Diplopoda, Julida) on Artemia salina.
EP  - 54
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2178
ER  - 

@conference{
author = "Milovanović, Jelena and Ilić, Bojan and Makarov, Slobodan and Đorđević Aleksić, Jelena and Stevanović, Aleksandra and Malešević, Anastasija and Vuković-Gačić, Branka",
year = "2021",
abstract = "INTRODUCTION:
Different orders within the class Diplopoda possess a variety of chemical compounds in their defense secretions: quinones, phenolics, alcohols, aldehydes, ketones, esters, alkaloids, cyanogenic compounds. Defensive secretions of species from the order Julida are regarded as the most complex within Diplopoda, and they are blends of several classes of chemical com- pounds: quinones, esters and ketones. Numerous biological activities of these secretions have been reported: antimicrobial, antioxidative, antineurodegenerative, cytotoxic and embryotoxic on zebrafish. Besides zebrafish embryos, Artemia salina is one of the common model organisms in toxicity assessment which has not been used for screening of toxicity of millipedes’ defensive secretions.
OBJECTIVES:
The main goal of this study was to examine the toxic effects of defensive secretions of two species from the order Julida [Megaphyllum bosniense (MBO) and M. unilineatum (MUN)] using Artemia salina (ARC test).
METHOD / DESIGN:
Adult individuals of M. bosniense were collected during April and May of 2021 on Mt. Avala, near Belgrade, while adults of M. unilineatum were collected during the same period in the Krnjača, suburb of Belgrade. After the capture, millipedes were kept in plastic boxes containing ground cover from the collecting site. The boxes were regularly sprayed with water to maintain high humidity. Due to the fact that the sample was female-biased, defensive secretions of female specimens were used for further analyses. Excretion of defensive secretions was elicited from glands of mentioned species via mechanical stress in closed glass vials. Secretions collected from both species were dissolved in 10 ml of hexane, concentrated under reduced pressure in a rotary evaporator (Rotavapor R-210, Buchi) at 40°C to a dry residue, and redissolved in 50% dimethylsulphoxide (DMSO). The stock concentration of extracts used in ARC test was 20 mg/mL. Before treatments, eggs of A. salina were incubated for 72h with constant lighting and aeration. For the purposes of the experiment, stage II and III larvae were used (separated by phototaxis in 300 ml of seawater). In a plate with 24 wells, 900 μl of seawater with larvae (10-15 per well) was placed and then 100 μl of tested extracts (range of concentrations 0,1 mg/mL - 0,003125 mg/mL) was added. Potassium dichromate (K2Cr2O7) was used as a positive control and DMSO was used as solvent control. The total number of individuals per well was counted after 24h and 48h, as well as the number of living and dead individuals. These data were used for esti- mation of survival rate and determination of LC50 value. The experiment was done in triplicate.
RESULTS:
Our results show that secretions from both species exhibit a toxic effect on the survival of the chosen model organism, with the MBO extract showing weaker activity in comparison with MUN extract. The LC50 value after 24h was about the same for both species (LC50=73,23 μg/mL for MBO and LC50=68,56 μg/mL for MUN). The LC50 value for MBO after 48h was 47,18 μg/ mL, while LC50 value in the same period for MUN was 29,12 μg/mL. Positive control (LC50= 13,5 μg/mL) showed three times stronger effects in relation to MBO and twice as strong when compared to MUN extract. It has also been shown that the num- ber of surviving individuals decreases with increasing concentration of tested extracts and the increasing incubation time.
CONCLUSIONS:
The defense secretions of both tested millipede species show toxic effects in the ARC test. It is shown that MBO extract has a weaker toxic effect than the MUN extract. This result can be linked with the fact that esters of long-chain fatty acids are dominant compounds in MBO, while MUN is almost exclusively benzoquinone-based. Esters detected in MBO are generally regarded as low-toxic compounds, but with the potential to interact with compounds from other chemical classes. However, as MBO achieved toxic effects and many esters that are detected in MBO are new natural products and their biological potential is unknown, further extensive studies are needed to determine their toxicological potential.",
publisher = "The International Bioscience Conference (2021; Novi Sad)",
journal = "International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021",
title = "Acute toxicity assessment of defense secretions of Megaphyllum bosniense (Verhoeff, 1897) and M. unilineatum (C. L. Koch, 1838) (Diplopoda, Julida) on Artemia salina.",
pages = "54-53",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2178"
}

Milovanović, J., Ilić, B., Makarov, S., Đorđević Aleksić, J., Stevanović, A., Malešević, A.,& Vuković-Gačić, B.. (2021). Acute toxicity assessment of defense secretions of Megaphyllum bosniense (Verhoeff, 1897) and M. unilineatum (C. L. Koch, 1838) (Diplopoda, Julida) on Artemia salina.. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021
The International Bioscience Conference (2021; Novi Sad)., 53-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2178

Milovanović J, Ilić B, Makarov S, Đorđević Aleksić J, Stevanović A, Malešević A, Vuković-Gačić B. Acute toxicity assessment of defense secretions of Megaphyllum bosniense (Verhoeff, 1897) and M. unilineatum (C. L. Koch, 1838) (Diplopoda, Julida) on Artemia salina.. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021. 2021;:53-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2178 .

Milovanović, Jelena, Ilić, Bojan, Makarov, Slobodan, Đorđević Aleksić, Jelena, Stevanović, Aleksandra, Malešević, Anastasija, Vuković-Gačić, Branka, "Acute toxicity assessment of defense secretions of Megaphyllum bosniense (Verhoeff, 1897) and M. unilineatum (C. L. Koch, 1838) (Diplopoda, Julida) on Artemia salina." in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021 (2021):53-54,
https://hdl.handle.net/21.15107/rcub_rimsi_2178 .

TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Stevanović, Aleksandra
AU  - Malešević, Anastasija
AU  - Vuković-Gačić, Branka
AU  - Milovanović, Jelena
AU  - Ilić, Bojan
AU  - Makarov, Slobodan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2177
AB  - INTRODUCTION:
In recent years, the emphasis of the scientific community has been placed on the invention of new antimicrobial agents due to the increasing resistance of bacteria to antibiotics. However, serious global health concern is focused on bacterial biofilms, a complex structure of a microbiome made up of colonies of bacteria or individual bacterial cells in a group, attached to a surface. Bacterial biofilms are highly resistant to antimicrobial agents and grow on the surfaces of medical implants such as sutures, catheters, and dental implants. Given that plants and animals are a valuable source of natural biologically active products, they are a good basis for finding new antimicrobial and antibiofilm agents. Bacterial strains of Pseudomonas aerug- inosa PAO1 and Staphylococcus aureus are known for biofilm production and cause opportunistic and chronic infections in humans, some of which are due to biofilm production. Due to their characteristic way of life, millipedes (Diplopoda) are char- acterized by a diverse and complex defense against predators, which includes the secretion of various chemical compounds that are toxic, repellent, or tasteless to predators. Analyzes have shown that millipedes produce chemical compounds such as phenols, alkaloids, quinones, terpenoids, cyanogenic compounds, and fatty acid esters, which showed antimicrobial ac- tivity, among other. Representatives of the order Julida, which are frequent in Republic of Serbia, produce defense secretions that are chemically very complex (the most complex within Diplopoda) and exhibit antimicrobial, antioxidant, and neurode- generative potential, so they represent a good basis for the invention of new antibiofilm agents.
OBJECTIVES:
Objectives are to determine the inhibition of biofilm formation and degradation of the formed biofilm of P. aeruginosa PAO1 and S. aureus by defense secretions of selected millipede species from the family Julidae as well as to determine their anti- microbial activity.
METHOD / DESIGN:
Biofilm formation was quantified by the crystal violet staining method, while antimicrobial activity was examined using the broth dilution minimum inhibitory concentration (MIC) test.
RESULTS:
Defensive secretions of Megaphyllum unilineatum (MUN), Pachyiulus hungaricus (PHU), and Cylindroiulus boleti (CBO) showedantimicrobial activity against S. aureus with MIC values of 0.03, 0.06, and 0.06 mg/mL, respectively. On P. aeruginosa PAO1, defense secretions did not show antimicrobial activity even at the highest tested concentration of 1 mg/mL for MUN while for PHU and CBO the MIC was 1 mg/mL, which is most likely due to the high resistance of this bacterial strain. The antibiofilm effect was observed in all tested defense secretions and was more pronounced against S. aureus than against P. aeruginosa PAO1. The strongest biofilm inhibition of S. aureus was at the highest tested concentrations (2 × MIC) with percentages of inhibition of CBO: 88.6%, PHU: 73.7%, and MUN: 67.2%. Degradation of already formed S. aureus biofilm was shown at lower tested defensive secretions concentrations (MIC/4), about 40% of biofilm degradation for MUN and PHU and about 30% for CBO. The strongest inhibition of P. aeruginosa PAO1 biofilm formation was observed at the highest tested concentrations of defensive secretions, 1 and 0.5 mg/mL for PHU (82 and 54%), and CBO (64.3 and 38.5%) while MUN had the strongest activity at the lowest tested concentration of 0.06 mg/mL (34.3%). All examined defense secretions had similar degradation activity of P. aeruginosa PAO1 biofilm with stronger activity at lower tested concentrations (about 30%). Defensive secretions of MUN and PHU extracted in DMSO solvent showed a stronger antibiofilm effect compared to the same ethanol extracts.
CONCLUSIONS:
The defense secretions of MBO, PHU, and CBO show a good basis for further investigations of their use as antimicrobial agents, especially against S. aureus.
PB  - Joint international PSU-UNS Bioscience Conference
C3  - International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021
T1  - Defensive secretions of millipedes Megaphyllum unilineatum (C. L. Koch, 1838), Pachyiulus hungaricus (Karsch, 1881) and Cylindroiulus boleti (C. L. Koch, 1847) (Diplopoda, Julida) as antimicrobial agents in the inhibition of biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus
EP  - 147
SP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2177
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Stevanović, Aleksandra and Malešević, Anastasija and Vuković-Gačić, Branka and Milovanović, Jelena and Ilić, Bojan and Makarov, Slobodan",
year = "2021",
abstract = "INTRODUCTION:
In recent years, the emphasis of the scientific community has been placed on the invention of new antimicrobial agents due to the increasing resistance of bacteria to antibiotics. However, serious global health concern is focused on bacterial biofilms, a complex structure of a microbiome made up of colonies of bacteria or individual bacterial cells in a group, attached to a surface. Bacterial biofilms are highly resistant to antimicrobial agents and grow on the surfaces of medical implants such as sutures, catheters, and dental implants. Given that plants and animals are a valuable source of natural biologically active products, they are a good basis for finding new antimicrobial and antibiofilm agents. Bacterial strains of Pseudomonas aerug- inosa PAO1 and Staphylococcus aureus are known for biofilm production and cause opportunistic and chronic infections in humans, some of which are due to biofilm production. Due to their characteristic way of life, millipedes (Diplopoda) are char- acterized by a diverse and complex defense against predators, which includes the secretion of various chemical compounds that are toxic, repellent, or tasteless to predators. Analyzes have shown that millipedes produce chemical compounds such as phenols, alkaloids, quinones, terpenoids, cyanogenic compounds, and fatty acid esters, which showed antimicrobial ac- tivity, among other. Representatives of the order Julida, which are frequent in Republic of Serbia, produce defense secretions that are chemically very complex (the most complex within Diplopoda) and exhibit antimicrobial, antioxidant, and neurode- generative potential, so they represent a good basis for the invention of new antibiofilm agents.
OBJECTIVES:
Objectives are to determine the inhibition of biofilm formation and degradation of the formed biofilm of P. aeruginosa PAO1 and S. aureus by defense secretions of selected millipede species from the family Julidae as well as to determine their anti- microbial activity.
METHOD / DESIGN:
Biofilm formation was quantified by the crystal violet staining method, while antimicrobial activity was examined using the broth dilution minimum inhibitory concentration (MIC) test.
RESULTS:
Defensive secretions of Megaphyllum unilineatum (MUN), Pachyiulus hungaricus (PHU), and Cylindroiulus boleti (CBO) showedantimicrobial activity against S. aureus with MIC values of 0.03, 0.06, and 0.06 mg/mL, respectively. On P. aeruginosa PAO1, defense secretions did not show antimicrobial activity even at the highest tested concentration of 1 mg/mL for MUN while for PHU and CBO the MIC was 1 mg/mL, which is most likely due to the high resistance of this bacterial strain. The antibiofilm effect was observed in all tested defense secretions and was more pronounced against S. aureus than against P. aeruginosa PAO1. The strongest biofilm inhibition of S. aureus was at the highest tested concentrations (2 × MIC) with percentages of inhibition of CBO: 88.6%, PHU: 73.7%, and MUN: 67.2%. Degradation of already formed S. aureus biofilm was shown at lower tested defensive secretions concentrations (MIC/4), about 40% of biofilm degradation for MUN and PHU and about 30% for CBO. The strongest inhibition of P. aeruginosa PAO1 biofilm formation was observed at the highest tested concentrations of defensive secretions, 1 and 0.5 mg/mL for PHU (82 and 54%), and CBO (64.3 and 38.5%) while MUN had the strongest activity at the lowest tested concentration of 0.06 mg/mL (34.3%). All examined defense secretions had similar degradation activity of P. aeruginosa PAO1 biofilm with stronger activity at lower tested concentrations (about 30%). Defensive secretions of MUN and PHU extracted in DMSO solvent showed a stronger antibiofilm effect compared to the same ethanol extracts.
CONCLUSIONS:
The defense secretions of MBO, PHU, and CBO show a good basis for further investigations of their use as antimicrobial agents, especially against S. aureus.",
publisher = "Joint international PSU-UNS Bioscience Conference",
journal = "International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021",
title = "Defensive secretions of millipedes Megaphyllum unilineatum (C. L. Koch, 1838), Pachyiulus hungaricus (Karsch, 1881) and Cylindroiulus boleti (C. L. Koch, 1847) (Diplopoda, Julida) as antimicrobial agents in the inhibition of biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus",
pages = "147-146",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2177"
}

Đorđević Aleksić, J., Stevanović, A., Malešević, A., Vuković-Gačić, B., Milovanović, J., Ilić, B.,& Makarov, S.. (2021). Defensive secretions of millipedes Megaphyllum unilineatum (C. L. Koch, 1838), Pachyiulus hungaricus (Karsch, 1881) and Cylindroiulus boleti (C. L. Koch, 1847) (Diplopoda, Julida) as antimicrobial agents in the inhibition of biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021
Joint international PSU-UNS Bioscience Conference., 146-147.
https://hdl.handle.net/21.15107/rcub_rimsi_2177

Đorđević Aleksić J, Stevanović A, Malešević A, Vuković-Gačić B, Milovanović J, Ilić B, Makarov S. Defensive secretions of millipedes Megaphyllum unilineatum (C. L. Koch, 1838), Pachyiulus hungaricus (Karsch, 1881) and Cylindroiulus boleti (C. L. Koch, 1847) (Diplopoda, Julida) as antimicrobial agents in the inhibition of biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus. in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021. 2021;:146-147.
https://hdl.handle.net/21.15107/rcub_rimsi_2177 .

Đorđević Aleksić, Jelena, Stevanović, Aleksandra, Malešević, Anastasija, Vuković-Gačić, Branka, Milovanović, Jelena, Ilić, Bojan, Makarov, Slobodan, "Defensive secretions of millipedes Megaphyllum unilineatum (C. L. Koch, 1838), Pachyiulus hungaricus (Karsch, 1881) and Cylindroiulus boleti (C. L. Koch, 1847) (Diplopoda, Julida) as antimicrobial agents in the inhibition of biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus" in International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference – IBSC2021 (2021):146-147,
https://hdl.handle.net/21.15107/rcub_rimsi_2177 .

TY  - CONF
AU  - Jović, Đorđe
AU  - Dražić, Gordana
AU  - Dražić, Dragana
AU  - Milovanović, Jelena
AU  - Stanković, Dragica
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2130
AB  - The paper presents a special evaluation of environmental, social and economic
functions of forests by applying Vyskot's methodologies of objectification,
systematization and evaluation of forest functions. To the method utilization, area
of Mountain Kosmaj is chosen, where the processes of urbanization have caused
the greatest change in use of natural resources, especially forests. For this reason,
the need for multi-functional use of forests is justified. Applying the methodology
of objectivity, systematization and evaluation of forest functions (Vyskot et al,
2003), a financial evaluation of forest functions is carried out. As a special part of
the research, determination of the level of contamination by heavy metals (Pb, Ni,
Mn, Zn and Fe, ), and their accumulation in plants on the mountain Kosmaj was
carried out. Evaluation of forest functions of the study area have been based on
data on forest area that have taken from the General management plan for
“Posavsko – podunavsko forest area (2001-2010)“ The financial value of each
function is expressed in € per m3, which is currently the only reliable method of
assessing functions. Use of this method, and data received form this research in
area of Mountain Kosmaj creates the basis for the implementation of the concept
of functionally integrated forest management. We will receive a more reliable
data for managing state forests, as well as data to perform inspection of the state
forests. There will be more reliable data to be used for forest management
planning, silviculture, forest objective categorization.
PB  - Faculty of Applied Ecology Futura, Belgrade
C3  - International Conference Ecological improvement of devastated locations for sustainable development Faculty of Applied Ecology Futura University Singidunum Belgrade, September 29th and 30th 2014
T1  - EVALUATION OF FORESTS IN THE AREA OF MOUNTAIN KOSMAJ IN RELATION TO THEIR ENVIRONMENTAL, SOCIAL AND ECONOMIC FUNCTIONS
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2130
ER  - 

@conference{
author = "Jović, Đorđe and Dražić, Gordana and Dražić, Dragana and Milovanović, Jelena and Stanković, Dragica",
year = "2014",
abstract = "The paper presents a special evaluation of environmental, social and economic
functions of forests by applying Vyskot's methodologies of objectification,
systematization and evaluation of forest functions. To the method utilization, area
of Mountain Kosmaj is chosen, where the processes of urbanization have caused
the greatest change in use of natural resources, especially forests. For this reason,
the need for multi-functional use of forests is justified. Applying the methodology
of objectivity, systematization and evaluation of forest functions (Vyskot et al,
2003), a financial evaluation of forest functions is carried out. As a special part of
the research, determination of the level of contamination by heavy metals (Pb, Ni,
Mn, Zn and Fe, ), and their accumulation in plants on the mountain Kosmaj was
carried out. Evaluation of forest functions of the study area have been based on
data on forest area that have taken from the General management plan for
“Posavsko – podunavsko forest area (2001-2010)“ The financial value of each
function is expressed in € per m3, which is currently the only reliable method of
assessing functions. Use of this method, and data received form this research in
area of Mountain Kosmaj creates the basis for the implementation of the concept
of functionally integrated forest management. We will receive a more reliable
data for managing state forests, as well as data to perform inspection of the state
forests. There will be more reliable data to be used for forest management
planning, silviculture, forest objective categorization.",
publisher = "Faculty of Applied Ecology Futura, Belgrade",
journal = "International Conference Ecological improvement of devastated locations for sustainable development Faculty of Applied Ecology Futura University Singidunum Belgrade, September 29th and 30th 2014",
title = "EVALUATION OF FORESTS IN THE AREA OF MOUNTAIN KOSMAJ IN RELATION TO THEIR ENVIRONMENTAL, SOCIAL AND ECONOMIC FUNCTIONS",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2130"
}

Jović, Đ., Dražić, G., Dražić, D., Milovanović, J.,& Stanković, D.. (2014). EVALUATION OF FORESTS IN THE AREA OF MOUNTAIN KOSMAJ IN RELATION TO THEIR ENVIRONMENTAL, SOCIAL AND ECONOMIC FUNCTIONS. in International Conference Ecological improvement of devastated locations for sustainable development Faculty of Applied Ecology Futura University Singidunum Belgrade, September 29th and 30th 2014
Faculty of Applied Ecology Futura, Belgrade., 69.
https://hdl.handle.net/21.15107/rcub_rimsi_2130

Jović Đ, Dražić G, Dražić D, Milovanović J, Stanković D. EVALUATION OF FORESTS IN THE AREA OF MOUNTAIN KOSMAJ IN RELATION TO THEIR ENVIRONMENTAL, SOCIAL AND ECONOMIC FUNCTIONS. in International Conference Ecological improvement of devastated locations for sustainable development Faculty of Applied Ecology Futura University Singidunum Belgrade, September 29th and 30th 2014. 2014;:69.
https://hdl.handle.net/21.15107/rcub_rimsi_2130 .

Jović, Đorđe, Dražić, Gordana, Dražić, Dragana, Milovanović, Jelena, Stanković, Dragica, "EVALUATION OF FORESTS IN THE AREA OF MOUNTAIN KOSMAJ IN RELATION TO THEIR ENVIRONMENTAL, SOCIAL AND ECONOMIC FUNCTIONS" in International Conference Ecological improvement of devastated locations for sustainable development Faculty of Applied Ecology Futura University Singidunum Belgrade, September 29th and 30th 2014 (2014):69,
https://hdl.handle.net/21.15107/rcub_rimsi_2130 .

TY  - JOUR
AU  - Šijačić-Nikolić, Mirjana
AU  - Milovanović, Jelena
AU  - Nonic, Marina
AU  - Knezević, Radmila
AU  - Stanković, Dragica
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/714
AB  - The taxonomic status of beech from the Balkan Peninsula is not yet clearly defined. There is no agreement among different authors about the morphological characteristics discriminating between the Balkan and European and/or Eastern beech. For most characteristics, the mean values are different but the ranges of variation overlap considerably. Provenance trial of beech established in Serbia, at the locality Debeli Lug, has provided an opportunity for research of inter-provenance variability at the level of leaf morphometric characteristics in juvenile development stage. Research included 10 provenances originating from the Western Balkans (Serbian provenance 36 and 38; Croatian provenance 24 and 25; Bosnian provenance 30 and 32) and from Central Europe (German provenance 47 and 49; Austrian provenance 56 and Hungarian provenance 42), where following morphometric characteristics were analyzed: leaf length (Ll), leaf width (Lw), petiole lenght (Pl), leaf base width on 1 cm (Blw), number of veins-left (Vl), number of veins - right (Vr), distance between 3(rd) and 4(th) vein - left (Dv 3-4). The results of this research show existence of clear differentiation among provenances from the Western Balkan and from Central Europe, from the point of leaf dimensions, number of veins and leaf base width.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Leaf morphometric characteristics variability of different beech provenances in juvenile development stage
EP  - 380
IS  - 2
SP  - 369
VL  - 45
DO  - 10.2298/GENSR1302369S
ER  - 

@article{
author = "Šijačić-Nikolić, Mirjana and Milovanović, Jelena and Nonic, Marina and Knezević, Radmila and Stanković, Dragica",
year = "2013",
abstract = "The taxonomic status of beech from the Balkan Peninsula is not yet clearly defined. There is no agreement among different authors about the morphological characteristics discriminating between the Balkan and European and/or Eastern beech. For most characteristics, the mean values are different but the ranges of variation overlap considerably. Provenance trial of beech established in Serbia, at the locality Debeli Lug, has provided an opportunity for research of inter-provenance variability at the level of leaf morphometric characteristics in juvenile development stage. Research included 10 provenances originating from the Western Balkans (Serbian provenance 36 and 38; Croatian provenance 24 and 25; Bosnian provenance 30 and 32) and from Central Europe (German provenance 47 and 49; Austrian provenance 56 and Hungarian provenance 42), where following morphometric characteristics were analyzed: leaf length (Ll), leaf width (Lw), petiole lenght (Pl), leaf base width on 1 cm (Blw), number of veins-left (Vl), number of veins - right (Vr), distance between 3(rd) and 4(th) vein - left (Dv 3-4). The results of this research show existence of clear differentiation among provenances from the Western Balkan and from Central Europe, from the point of leaf dimensions, number of veins and leaf base width.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Leaf morphometric characteristics variability of different beech provenances in juvenile development stage",
pages = "380-369",
number = "2",
volume = "45",
doi = "10.2298/GENSR1302369S"
}

Šijačić-Nikolić, M., Milovanović, J., Nonic, M., Knezević, R.,& Stanković, D.. (2013). Leaf morphometric characteristics variability of different beech provenances in juvenile development stage. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 45(2), 369-380.
https://doi.org/10.2298/GENSR1302369S

Šijačić-Nikolić M, Milovanović J, Nonic M, Knezević R, Stanković D. Leaf morphometric characteristics variability of different beech provenances in juvenile development stage. in Genetika-Belgrade. 2013;45(2):369-380.
doi:10.2298/GENSR1302369S .

Šijačić-Nikolić, Mirjana, Milovanović, Jelena, Nonic, Marina, Knezević, Radmila, Stanković, Dragica, "Leaf morphometric characteristics variability of different beech provenances in juvenile development stage" in Genetika-Belgrade, 45, no. 2 (2013):369-380,
https://doi.org/10.2298/GENSR1302369S . .

TY  - CONF
AU  - Šijačić-Nikolić, Mirjana
AU  - Vilotić, Dragica
AU  - Milovanović, Jelena
AU  - Stanković, Dragica
PY  - 2011
PB  - The 9th International Beech Symposium: Ecology and Silviculture of Beech, Dresden, Germany,
C3  - IUFRO, Germany
T1  - SPRING PHENOLOGY OF EUROPEAN BEECH (FAGUS SYLVATICA L.) PROVENANCES WITHIN INTERNATIONAL PROVENANCE TRIAL IN SERBIA
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2431
ER  - 

@conference{
author = "Šijačić-Nikolić, Mirjana and Vilotić, Dragica and Milovanović, Jelena and Stanković, Dragica",
year = "2011",
publisher = "The 9th International Beech Symposium: Ecology and Silviculture of Beech, Dresden, Germany,",
journal = "IUFRO, Germany",
title = "SPRING PHENOLOGY OF EUROPEAN BEECH (FAGUS SYLVATICA L.) PROVENANCES WITHIN INTERNATIONAL PROVENANCE TRIAL IN SERBIA",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2431"
}

Šijačić-Nikolić, M., Vilotić, D., Milovanović, J.,& Stanković, D.. (2011). SPRING PHENOLOGY OF EUROPEAN BEECH (FAGUS SYLVATICA L.) PROVENANCES WITHIN INTERNATIONAL PROVENANCE TRIAL IN SERBIA. in IUFRO, Germany
The 9th International Beech Symposium: Ecology and Silviculture of Beech, Dresden, Germany,..
https://hdl.handle.net/21.15107/rcub_rimsi_2431

Šijačić-Nikolić M, Vilotić D, Milovanović J, Stanković D. SPRING PHENOLOGY OF EUROPEAN BEECH (FAGUS SYLVATICA L.) PROVENANCES WITHIN INTERNATIONAL PROVENANCE TRIAL IN SERBIA. in IUFRO, Germany. 2011;.
https://hdl.handle.net/21.15107/rcub_rimsi_2431 .

Šijačić-Nikolić, Mirjana, Vilotić, Dragica, Milovanović, Jelena, Stanković, Dragica, "SPRING PHENOLOGY OF EUROPEAN BEECH (FAGUS SYLVATICA L.) PROVENANCES WITHIN INTERNATIONAL PROVENANCE TRIAL IN SERBIA" in IUFRO, Germany (2011),
https://hdl.handle.net/21.15107/rcub_rimsi_2431 .

TY  - JOUR
AU  - Šijačić-Nikolić, Mirjana
AU  - Vilotić, Dragica
AU  - Milovanović, Jelena
AU  - Veselinović, Milorad
AU  - Stanković, Dragica
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2267
PB  - Freising : Parlar Scientific Publications
T2  - Fresenius environmental bulletin
T1  - Application of superabsorbent polymers in the production of Scot pine (Pinus sylvestris L.) and Austrian pine (Pinus nigra Arn.) seedlings
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2267
ER  - 

@article{
author = "Šijačić-Nikolić, Mirjana and Vilotić, Dragica and Milovanović, Jelena and Veselinović, Milorad and Stanković, Dragica",
year = "2010",
publisher = "Freising : Parlar Scientific Publications",
journal = "Fresenius environmental bulletin",
title = "Application of superabsorbent polymers in the production of Scot pine (Pinus sylvestris L.) and Austrian pine (Pinus nigra Arn.) seedlings",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2267"
}

Šijačić-Nikolić, M., Vilotić, D., Milovanović, J., Veselinović, M.,& Stanković, D.. (2010). Application of superabsorbent polymers in the production of Scot pine (Pinus sylvestris L.) and Austrian pine (Pinus nigra Arn.) seedlings. in Fresenius environmental bulletin
Freising : Parlar Scientific Publications..
https://hdl.handle.net/21.15107/rcub_rimsi_2267

Šijačić-Nikolić M, Vilotić D, Milovanović J, Veselinović M, Stanković D. Application of superabsorbent polymers in the production of Scot pine (Pinus sylvestris L.) and Austrian pine (Pinus nigra Arn.) seedlings. in Fresenius environmental bulletin. 2010;.
https://hdl.handle.net/21.15107/rcub_rimsi_2267 .

Šijačić-Nikolić, Mirjana, Vilotić, Dragica, Milovanović, Jelena, Veselinović, Milorad, Stanković, Dragica, "Application of superabsorbent polymers in the production of Scot pine (Pinus sylvestris L.) and Austrian pine (Pinus nigra Arn.) seedlings" in Fresenius environmental bulletin (2010),
https://hdl.handle.net/21.15107/rcub_rimsi_2267 .

TY  - JOUR
AU  - Šijačić-Nikolić, Mirjana
AU  - Vilotić, Dragica
AU  - Milovanović, Jelena
AU  - Veselinović, Milorad
AU  - Stanković, Dragica
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/383
AB  - The effect of superabsorbent polymers on Scotch pine (Pinus sylvestris L.) and Austrian pine (Pinus nigra Am.) seed germination and the development of 1-year-old- and 2-year-old seedlings were analyzed. The study results show a positive effect of polymers on seedling development of model species, so they can be considered to be a good recommendation for a wider implementation in seedling production of coniferous tree species.
PB  - Parlar Scientific Publications (P S P), Freising
T2  - Fresenius Environmental Bulletin
T1  - Application of superabsorbent polymers in the production of scotch pine (pinus sylvestris l.) And Austrian pine (pinus nigra arn.) Seedlings
EP  - 1185
IS  - 6
SP  - 1180
VL  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_383
ER  - 

@article{
author = "Šijačić-Nikolić, Mirjana and Vilotić, Dragica and Milovanović, Jelena and Veselinović, Milorad and Stanković, Dragica",
year = "2010",
abstract = "The effect of superabsorbent polymers on Scotch pine (Pinus sylvestris L.) and Austrian pine (Pinus nigra Am.) seed germination and the development of 1-year-old- and 2-year-old seedlings were analyzed. The study results show a positive effect of polymers on seedling development of model species, so they can be considered to be a good recommendation for a wider implementation in seedling production of coniferous tree species.",
publisher = "Parlar Scientific Publications (P S P), Freising",
journal = "Fresenius Environmental Bulletin",
title = "Application of superabsorbent polymers in the production of scotch pine (pinus sylvestris l.) And Austrian pine (pinus nigra arn.) Seedlings",
pages = "1185-1180",
number = "6",
volume = "19",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_383"
}

Šijačić-Nikolić, M., Vilotić, D., Milovanović, J., Veselinović, M.,& Stanković, D.. (2010). Application of superabsorbent polymers in the production of scotch pine (pinus sylvestris l.) And Austrian pine (pinus nigra arn.) Seedlings. in Fresenius Environmental Bulletin
Parlar Scientific Publications (P S P), Freising., 19(6), 1180-1185.
https://hdl.handle.net/21.15107/rcub_rimsi_383

Šijačić-Nikolić M, Vilotić D, Milovanović J, Veselinović M, Stanković D. Application of superabsorbent polymers in the production of scotch pine (pinus sylvestris l.) And Austrian pine (pinus nigra arn.) Seedlings. in Fresenius Environmental Bulletin. 2010;19(6):1180-1185.
https://hdl.handle.net/21.15107/rcub_rimsi_383 .

Šijačić-Nikolić, Mirjana, Vilotić, Dragica, Milovanović, Jelena, Veselinović, Milorad, Stanković, Dragica, "Application of superabsorbent polymers in the production of scotch pine (pinus sylvestris l.) And Austrian pine (pinus nigra arn.) Seedlings" in Fresenius Environmental Bulletin, 19, no. 6 (2010):1180-1185,
https://hdl.handle.net/21.15107/rcub_rimsi_383 .