Kracun-Kolarevic, Margareta

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  • Kracun-Kolarevic, Margareta (1)
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Significance of genotoxicity and toxicity evaluation of freshwater bodies

Sunjog, Karolina; Kolarević, Stoimir; Kracun-Kolarevic, Margareta; Kostić-Vuković, Jovana; Višnjić Jeftić, Željka; Gačić, Zoran; Lenhardt, Mirjana; Vuković-Gačić, Branka

(Institute of Marine Biology, University of Montenegro, 2019)

TY  - CONF
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1736
AB  - Fish communities are excellent indicators of biological and ecological integrity due to 
their continuous exposure to water conditions. Freshwater biodiversity constitute a valu able natural resource but extensive pollution of freshwater can result in biodiversity de cline. For that reason it is important to employ bioassays for purpose of detecting these 
conditions. In this work we monitored chub and bream species in 2 reservoirs, Uvac and 
Garasi, and 4 rivers: Dunav, Sava, Pestan and Beljanica. For assessment of metal and 
metalloid in fish tissues (liver, gills, gonads and muscle) we have used ICP-OES. The 
comet assay or single cell gel electrophoresis (SCGE) was selected as an in vivo geno toxicity assay, for measuring DNA damage in blood, liver and gills. Histopathological 
alterations were monitored in liver and gills.The high quality of Uvac water was con firmed by low values of DNA damage in all tissues compared to other sites. An analysis 
metals in tissues showed a high degree of their differentiation, as well as significant dif ferences in the distribution of tissue elements between the sites tested. The highest con centrations of most of the analyzed metals were found in gills, liver and gonads, and the 
lowest in muscles at all sites. In chub, blood showed the lowest DNA damage compared 
to liver and gills, while in all breams DNA damage was the highest in blood cells, follow ing gills and liver. Histopathological analysis performed on bream specimens on the Sava 
and Danube River revealed a higher degree of alterations in liver compared to the gills.
PB  - Institute of Marine Biology, University of Montenegro
C3  - International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.
T1  - Significance of genotoxicity and toxicity evaluation of freshwater bodies
SP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1736
ER  - 
@conference{
author = "Sunjog, Karolina and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Kostić-Vuković, Jovana and Višnjić Jeftić, Željka and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2019",
abstract = "Fish communities are excellent indicators of biological and ecological integrity due to 
their continuous exposure to water conditions. Freshwater biodiversity constitute a valu able natural resource but extensive pollution of freshwater can result in biodiversity de cline. For that reason it is important to employ bioassays for purpose of detecting these 
conditions. In this work we monitored chub and bream species in 2 reservoirs, Uvac and 
Garasi, and 4 rivers: Dunav, Sava, Pestan and Beljanica. For assessment of metal and 
metalloid in fish tissues (liver, gills, gonads and muscle) we have used ICP-OES. The 
comet assay or single cell gel electrophoresis (SCGE) was selected as an in vivo geno toxicity assay, for measuring DNA damage in blood, liver and gills. Histopathological 
alterations were monitored in liver and gills.The high quality of Uvac water was con firmed by low values of DNA damage in all tissues compared to other sites. An analysis 
metals in tissues showed a high degree of their differentiation, as well as significant dif ferences in the distribution of tissue elements between the sites tested. The highest con centrations of most of the analyzed metals were found in gills, liver and gonads, and the 
lowest in muscles at all sites. In chub, blood showed the lowest DNA damage compared 
to liver and gills, while in all breams DNA damage was the highest in blood cells, follow ing gills and liver. Histopathological analysis performed on bream specimens on the Sava 
and Danube River revealed a higher degree of alterations in liver compared to the gills.",
publisher = "Institute of Marine Biology, University of Montenegro",
journal = "International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.",
title = "Significance of genotoxicity and toxicity evaluation of freshwater bodies",
pages = "114",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1736"
}
Sunjog, K., Kolarević, S., Kracun-Kolarevic, M., Kostić-Vuković, J., Višnjić Jeftić, Ž., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2019). Significance of genotoxicity and toxicity evaluation of freshwater bodies. in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.
Institute of Marine Biology, University of Montenegro., 114.
https://hdl.handle.net/21.15107/rcub_rimsi_1736
Sunjog K, Kolarević S, Kracun-Kolarevic M, Kostić-Vuković J, Višnjić Jeftić Ž, Gačić Z, Lenhardt M, Vuković-Gačić B. Significance of genotoxicity and toxicity evaluation of freshwater bodies. in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.. 2019;:114.
https://hdl.handle.net/21.15107/rcub_rimsi_1736 .
Sunjog, Karolina, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Kostić-Vuković, Jovana, Višnjić Jeftić, Željka, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Significance of genotoxicity and toxicity evaluation of freshwater bodies" in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro. (2019):114,
https://hdl.handle.net/21.15107/rcub_rimsi_1736 .