TY  - JOUR
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Despotović, Jovana
AU  - Loncarević, Branka
AU  - Ljesević, Marija
AU  - Spasić, Slađana
AU  - Nikolić, Aleksandra
AU  - Beskoski, Vladimir P.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1423
AB  - Cerium oxide nanoparticles (nCeO(2)) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO(2) on tested animals, but literature data are mainly contradictory. Coating of nCeO(2) can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO(2). Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO(2) in comparison to uncoated nCeO(2) using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO(2) on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO(2) on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO(2) on Vibrio fischeri. The coating of nCeO(2) did not affect the nanoparticles' accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO(2), while the crustacean's O-2 consumption was not affected by any of the coated nCeO(2). In summary, this study revealed that, at 200 mg L-1 uncoated and carbohydrate-coated nCeO(2) are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO(2). The highest production was in glucose and levan-coated nCeO(2) according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)
PB  - Elsevier, Amsterdam
T2  - Aquatic Toxicology
T1  - Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio
VL  - 236
DO  - 10.1016/j.aquatox.2021.105867
ER  - 

@article{
author = "Milenković, Ivana and Radotić, Ksenija and Despotović, Jovana and Loncarević, Branka and Ljesević, Marija and Spasić, Slađana and Nikolić, Aleksandra and Beskoski, Vladimir P.",
year = "2021",
abstract = "Cerium oxide nanoparticles (nCeO(2)) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO(2) on tested animals, but literature data are mainly contradictory. Coating of nCeO(2) can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO(2). Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO(2) in comparison to uncoated nCeO(2) using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO(2) on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO(2) on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO(2) on Vibrio fischeri. The coating of nCeO(2) did not affect the nanoparticles' accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO(2), while the crustacean's O-2 consumption was not affected by any of the coated nCeO(2). In summary, this study revealed that, at 200 mg L-1 uncoated and carbohydrate-coated nCeO(2) are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO(2). The highest production was in glucose and levan-coated nCeO(2) according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)",
publisher = "Elsevier, Amsterdam",
journal = "Aquatic Toxicology",
title = "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio",
volume = "236",
doi = "10.1016/j.aquatox.2021.105867"
}

Milenković, I., Radotić, K., Despotović, J., Loncarević, B., Ljesević, M., Spasić, S., Nikolić, A.,& Beskoski, V. P.. (2021). Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology
Elsevier, Amsterdam., 236.
https://doi.org/10.1016/j.aquatox.2021.105867

Milenković I, Radotić K, Despotović J, Loncarević B, Ljesević M, Spasić S, Nikolić A, Beskoski VP. Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology. 2021;236.
doi:10.1016/j.aquatox.2021.105867 .

Milenković, Ivana, Radotić, Ksenija, Despotović, Jovana, Loncarević, Branka, Ljesević, Marija, Spasić, Slađana, Nikolić, Aleksandra, Beskoski, Vladimir P., "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio" in Aquatic Toxicology, 236 (2021),
https://doi.org/10.1016/j.aquatox.2021.105867 . .

TY  - CONF
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Despotović, Jovana
AU  - Lončarević, Branka
AU  - Lješević, Marija
AU  - Nikolić, Aleksandra
AU  - Beškoski, Vladimir
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1840
AB  - The use of nanomaterials in various commercial products and industrial processes has increased. Although the application of nanoparticles has great importance, some of them can be risky to human health and the environment. Cerium oxide nanoparticles CeO2 have been extensively investigated due to the excellent oxygen storage capacities on the basis of the redox transition between Ce3+ and Ce4+ and formation of oxygen vacancies on their surface. The effect of CeO2 on individual organisms and the ecosystem in general are not sufficiently explored. In this research we used CeO2, naked and coated with three different carbohydrates (glucose, pullulan or levan), to study their effect in three different model systems. We analyzed bioluminescence in gram-negative bacterium Vibrio fischeri, and acute toxicity in crustacean Daphnia magna and zebrafish Danio rerio. In all experiments the concentration of CeO2 nanoparticles was 200 mg/L. For all used types of nanoparticles, we observed  bioluminescence inhibition of around 20%. The mortality rate of treated D. magna was 6.7% for glucose coated CeO2, 9.2% for naked and pullulan coated CeO2 and 18.2% for levan coated CeO2. Despite the adherence of nanoparticles aggregates to the outer surface of the chorion, no acute toxicity was observed for zebrafish embryos during the first 72 hours post fertilization. We also haven’t observed increased level of abnormalities among treated embryos during the first 72 hours post fertilization. In this study, we haven’t observed any toxic effect of CeO2 nanoparticles, but the extent of the nanoparticles’ uptake by the organism remains to be investigated. The effect of chronic exposure to CeO2 should also be analyzed.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Sixth Conference
T1  - In vivo toxicity of naked and coated CeO2 nanoparticles
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1840
ER  - 

@conference{
author = "Milenković, Ivana and Radotić, Ksenija and Despotović, Jovana and Lončarević, Branka and Lješević, Marija and Nikolić, Aleksandra and Beškoski, Vladimir",
year = "2016",
abstract = "The use of nanomaterials in various commercial products and industrial processes has increased. Although the application of nanoparticles has great importance, some of them can be risky to human health and the environment. Cerium oxide nanoparticles CeO2 have been extensively investigated due to the excellent oxygen storage capacities on the basis of the redox transition between Ce3+ and Ce4+ and formation of oxygen vacancies on their surface. The effect of CeO2 on individual organisms and the ecosystem in general are not sufficiently explored. In this research we used CeO2, naked and coated with three different carbohydrates (glucose, pullulan or levan), to study their effect in three different model systems. We analyzed bioluminescence in gram-negative bacterium Vibrio fischeri, and acute toxicity in crustacean Daphnia magna and zebrafish Danio rerio. In all experiments the concentration of CeO2 nanoparticles was 200 mg/L. For all used types of nanoparticles, we observed  bioluminescence inhibition of around 20%. The mortality rate of treated D. magna was 6.7% for glucose coated CeO2, 9.2% for naked and pullulan coated CeO2 and 18.2% for levan coated CeO2. Despite the adherence of nanoparticles aggregates to the outer surface of the chorion, no acute toxicity was observed for zebrafish embryos during the first 72 hours post fertilization. We also haven’t observed increased level of abnormalities among treated embryos during the first 72 hours post fertilization. In this study, we haven’t observed any toxic effect of CeO2 nanoparticles, but the extent of the nanoparticles’ uptake by the organism remains to be investigated. The effect of chronic exposure to CeO2 should also be analyzed.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Sixth Conference",
title = "In vivo toxicity of naked and coated CeO2 nanoparticles",
pages = "137",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1840"
}

Milenković, I., Radotić, K., Despotović, J., Lončarević, B., Lješević, M., Nikolić, A.,& Beškoski, V.. (2016). In vivo toxicity of naked and coated CeO2 nanoparticles. in Serbian Biochemical Society Sixth Conference
Faculty of Chemistry, Serbian Biochemical Society., 137.
https://hdl.handle.net/21.15107/rcub_rimsi_1840

Milenković I, Radotić K, Despotović J, Lončarević B, Lješević M, Nikolić A, Beškoski V. In vivo toxicity of naked and coated CeO2 nanoparticles. in Serbian Biochemical Society Sixth Conference. 2016;:137.
https://hdl.handle.net/21.15107/rcub_rimsi_1840 .

Milenković, Ivana, Radotić, Ksenija, Despotović, Jovana, Lončarević, Branka, Lješević, Marija, Nikolić, Aleksandra, Beškoski, Vladimir, "In vivo toxicity of naked and coated CeO2 nanoparticles" in Serbian Biochemical Society Sixth Conference (2016):137,
https://hdl.handle.net/21.15107/rcub_rimsi_1840 .