TY  - JOUR
AU  - Živanović, Bojana
AU  - Milić Komić, Sonja
AU  - Tosti, Tomislav
AU  - Vidović, Marija
AU  - Prokic, Ljiljana
AU  - Veljović-Jovanović, Sonja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1347
AB  - Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato
IS  - 9
VL  - 9
DO  - 10.3390/plants9091147
ER  - 

@article{
author = "Živanović, Bojana and Milić Komić, Sonja and Tosti, Tomislav and Vidović, Marija and Prokic, Ljiljana and Veljović-Jovanović, Sonja",
year = "2020",
abstract = "Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato",
number = "9",
volume = "9",
doi = "10.3390/plants9091147"
}

Živanović, B., Milić Komić, S., Tosti, T., Vidović, M., Prokic, L.,& Veljović-Jovanović, S.. (2020). Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants-Basel
MDPI, Basel., 9(9).
https://doi.org/10.3390/plants9091147

Živanović B, Milić Komić S, Tosti T, Vidović M, Prokic L, Veljović-Jovanović S. Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants-Basel. 2020;9(9).
doi:10.3390/plants9091147 .

Živanović, Bojana, Milić Komić, Sonja, Tosti, Tomislav, Vidović, Marija, Prokic, Ljiljana, Veljović-Jovanović, Sonja, "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato" in Plants-Basel, 9, no. 9 (2020),
https://doi.org/10.3390/plants9091147 . .

TY  - JOUR
AU  - Sedlarević Zorić, Ana
AU  - Morina, Filis
AU  - Tosevski, Ivo
AU  - Tosti, Tomislav
AU  - Jović, Jelena
AU  - Krstic, Oliver
AU  - Veljović-Jovanović, Sonja
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1253
AB  - Trehalose and its precursor, trehalose 6-phosphate (T6P), are essential regulators of plant response to abiotic and biotic stress. Here we used the specific host-insect interaction between Linaria vulgaris (Plantaginaceae) and stem-galling weevil, Rhinusa pilosa (Mecinini, Curculionidae) with the aim to distinguish carbohydrate allocation patterns in response to herbivory, gall formation (G1, 24 h after oviposition), and gall development (G2, 7 days after oviposition) under controlled conditions. The hypothesis is that herbivory and galling induce distinct responses in both leaves and stems, and that shifts in carbon allocations are regulated by signaling sugars. Systemic response to herbivory was accumulation of T6P and maltose. The main feature of G1 in the stems was accumulation of trehalose, accompanied by increased T6P, turanose and glucose content, oppositely to the leaves. In G2, galls had 3-folds higher weight than controls, with further accumulation of fructose, glucose, turanose, and total water-insoluble carbohydrates (TIC), while the sucrose/hexose ratio decreased. Analysis of fast chlorophyll fluorescence kinetic (OJIP) transients in G2 showed a slight decrease in quantum yield of electron transport flux from Q(A) to Q(B), and towards photosystem I acceptor side, correlated with the decreased content of photosynthetic pigments and hexoses accumulation. Redistribution of photosynthates, and accumulation of T6P were induced in response to herbivory, indicating its signaling role. The results support the hypothesis that R. pilosa can induce plant reprogramming towards the accumulation of beneficial carbohydrates in developing gall by mechanisms which include both T6P and trehalose.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Resource allocation in response to herbivory and gall formation in Linaria vulgaris
EP  - 232
SP  - 224
VL  - 135
DO  - 10.1016/j.plaphy.2018.11.032
ER  - 

@article{
author = "Sedlarević Zorić, Ana and Morina, Filis and Tosevski, Ivo and Tosti, Tomislav and Jović, Jelena and Krstic, Oliver and Veljović-Jovanović, Sonja",
year = "2019",
abstract = "Trehalose and its precursor, trehalose 6-phosphate (T6P), are essential regulators of plant response to abiotic and biotic stress. Here we used the specific host-insect interaction between Linaria vulgaris (Plantaginaceae) and stem-galling weevil, Rhinusa pilosa (Mecinini, Curculionidae) with the aim to distinguish carbohydrate allocation patterns in response to herbivory, gall formation (G1, 24 h after oviposition), and gall development (G2, 7 days after oviposition) under controlled conditions. The hypothesis is that herbivory and galling induce distinct responses in both leaves and stems, and that shifts in carbon allocations are regulated by signaling sugars. Systemic response to herbivory was accumulation of T6P and maltose. The main feature of G1 in the stems was accumulation of trehalose, accompanied by increased T6P, turanose and glucose content, oppositely to the leaves. In G2, galls had 3-folds higher weight than controls, with further accumulation of fructose, glucose, turanose, and total water-insoluble carbohydrates (TIC), while the sucrose/hexose ratio decreased. Analysis of fast chlorophyll fluorescence kinetic (OJIP) transients in G2 showed a slight decrease in quantum yield of electron transport flux from Q(A) to Q(B), and towards photosystem I acceptor side, correlated with the decreased content of photosynthetic pigments and hexoses accumulation. Redistribution of photosynthates, and accumulation of T6P were induced in response to herbivory, indicating its signaling role. The results support the hypothesis that R. pilosa can induce plant reprogramming towards the accumulation of beneficial carbohydrates in developing gall by mechanisms which include both T6P and trehalose.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Resource allocation in response to herbivory and gall formation in Linaria vulgaris",
pages = "232-224",
volume = "135",
doi = "10.1016/j.plaphy.2018.11.032"
}

Sedlarević Zorić, A., Morina, F., Tosevski, I., Tosti, T., Jović, J., Krstic, O.,& Veljović-Jovanović, S.. (2019). Resource allocation in response to herbivory and gall formation in Linaria vulgaris. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 135, 224-232.
https://doi.org/10.1016/j.plaphy.2018.11.032

Sedlarević Zorić A, Morina F, Tosevski I, Tosti T, Jović J, Krstic O, Veljović-Jovanović S. Resource allocation in response to herbivory and gall formation in Linaria vulgaris. in Plant Physiology and Biochemistry. 2019;135:224-232.
doi:10.1016/j.plaphy.2018.11.032 .

Sedlarević Zorić, Ana, Morina, Filis, Tosevski, Ivo, Tosti, Tomislav, Jović, Jelena, Krstic, Oliver, Veljović-Jovanović, Sonja, "Resource allocation in response to herbivory and gall formation in Linaria vulgaris" in Plant Physiology and Biochemistry, 135 (2019):224-232,
https://doi.org/10.1016/j.plaphy.2018.11.032 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Milić Komić, Sonja
AU  - Albert, Andreas
AU  - Zechmann, Bernd
AU  - Tosti, Tomislav
AU  - Winkler, Jana Barbro
AU  - Veljović-Jovanović, Sonja
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/858
AB  - We studied the specific effects of high photosynthetically active radiation (PAR, 400-700 nm) and ecologically relevant UV-B radiation (0.90 W m(-2)) on antioxidative and phenolic metabolism by exploiting the green-white leaf variegation of Pelargonium zonale plants. This is a suitable model system for examining "source-sink" interactions within the same leaf. High PAR intensity (1350 mu mol m(-2) s(-1)) and UV-B radiation induced different responses in green and white leaf sectors. High PAR intensity had a greater influence on green tissue, triggering the accumulation of phenylpropanoids and flavonoids with strong antioxidative function. Induced phenolics, together with ascorbate, ascorbate peroxidase (APX, EC 1.11.1.11) and catalase (CAT, EC 1.11.1.6) provided efficient defense against potential oxidative pressure. UV-B-induced up-regulation of non-phenolic H2O2 scavengers in green leaf sectors was greater than high PAR-induced changes, indicating a UV-B role in antioxidative defense under light excess; on the contrary, minimal effects were observed in white tissue. However, UV-B radiation had greater influence on phenolics in white leaf sections compared to green ones, inducing accumulation of phenolic glycosides whose function was UV-B screening rather than antioxidative. By stimulation of starch and sucrose breakdown and carbon allocation in the form of soluble sugars from "source" (green) tissue to "sink" (white) tissue, UV-B radiation compensated the absence of photosynthetic activity and phenylpropanoid and flavonoid biosynthesis in white sectors.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity
EP  - 55
SP  - 44
VL  - 93
DO  - 10.1016/j.plaphy.2015.01.008
ER  - 

@article{
author = "Vidović, Marija and Morina, Filis and Milić Komić, Sonja and Albert, Andreas and Zechmann, Bernd and Tosti, Tomislav and Winkler, Jana Barbro and Veljović-Jovanović, Sonja",
year = "2015",
abstract = "We studied the specific effects of high photosynthetically active radiation (PAR, 400-700 nm) and ecologically relevant UV-B radiation (0.90 W m(-2)) on antioxidative and phenolic metabolism by exploiting the green-white leaf variegation of Pelargonium zonale plants. This is a suitable model system for examining "source-sink" interactions within the same leaf. High PAR intensity (1350 mu mol m(-2) s(-1)) and UV-B radiation induced different responses in green and white leaf sectors. High PAR intensity had a greater influence on green tissue, triggering the accumulation of phenylpropanoids and flavonoids with strong antioxidative function. Induced phenolics, together with ascorbate, ascorbate peroxidase (APX, EC 1.11.1.11) and catalase (CAT, EC 1.11.1.6) provided efficient defense against potential oxidative pressure. UV-B-induced up-regulation of non-phenolic H2O2 scavengers in green leaf sectors was greater than high PAR-induced changes, indicating a UV-B role in antioxidative defense under light excess; on the contrary, minimal effects were observed in white tissue. However, UV-B radiation had greater influence on phenolics in white leaf sections compared to green ones, inducing accumulation of phenolic glycosides whose function was UV-B screening rather than antioxidative. By stimulation of starch and sucrose breakdown and carbon allocation in the form of soluble sugars from "source" (green) tissue to "sink" (white) tissue, UV-B radiation compensated the absence of photosynthetic activity and phenylpropanoid and flavonoid biosynthesis in white sectors.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity",
pages = "55-44",
volume = "93",
doi = "10.1016/j.plaphy.2015.01.008"
}

Vidović, M., Morina, F., Milić Komić, S., Albert, A., Zechmann, B., Tosti, T., Winkler, J. B.,& Veljović-Jovanović, S.. (2015). Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 93, 44-55.
https://doi.org/10.1016/j.plaphy.2015.01.008

Vidović M, Morina F, Milić Komić S, Albert A, Zechmann B, Tosti T, Winkler JB, Veljović-Jovanović S. Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity. in Plant Physiology and Biochemistry. 2015;93:44-55.
doi:10.1016/j.plaphy.2015.01.008 .

Vidović, Marija, Morina, Filis, Milić Komić, Sonja, Albert, Andreas, Zechmann, Bernd, Tosti, Tomislav, Winkler, Jana Barbro, Veljović-Jovanović, Sonja, "Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity" in Plant Physiology and Biochemistry, 93 (2015):44-55,
https://doi.org/10.1016/j.plaphy.2015.01.008 . .