TY  - JOUR
AU  - Chatzopoulou, Fani
AU  - Sanmartin, Maite
AU  - Mellidou, Ifigeneia
AU  - Pateraki, Irini
AU  - Koukounaras, Athanasios
AU  - Tanou, Georgia
AU  - Kalamaki, Mary S.
AU  - Veljović-Jovanović, Sonja
AU  - Cvetić-Antić, Tijana
AU  - Kostas, Stefanos
AU  - Tsouvaltzis, Pavlos
AU  - Grumet, Rebecca
AU  - Kanellis, Angelos K.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1327
AB  - Ascorbate oxidase (AO, EC 1.10.3.3) is a copper-containing enzyme localized at the apoplast, where it catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHA) via monodehydroascorbic acid (MDHA) intermediate. Despite it has been extensively studied, no biological roles have been definitively ascribed. To understand the role of AO in plant metabolism, fruit growth and physiology, we suppressed AO expression in melon (Cucumis melo L.) fruit. Reduction of AO activity increased AA content in melon fruit, which is the result of repression of AA oxidation and simultaneous induction of certain biosynthetic and recycling genes. As a consequence, ascorbate redox state was altered in the apoplast. Interestingly, transgenic melon fruit displayed increased ethylene production rate coincided with elevated levels of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO, EC 1.14.17.4) activity and gene expression, which might contribute to earlier ripening. Moreover, AO suppressed transgenic melon fruit exhibited a dramatic arrest in fruit growth, due to a simultaneous decrease in fruit cell size and in plasmalemma (PM) ATPase activity. All the above, support for the first time, the in vivo AO participation in the rapid fruit growth of Cucurbitaceae and further suggest an alternative route for AA increase in ripening fruit.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit
EP  - 303
SP  - 291
VL  - 156
DO  - 10.1016/j.plaphy.2020.08.040
ER  - 

@article{
author = "Chatzopoulou, Fani and Sanmartin, Maite and Mellidou, Ifigeneia and Pateraki, Irini and Koukounaras, Athanasios and Tanou, Georgia and Kalamaki, Mary S. and Veljović-Jovanović, Sonja and Cvetić-Antić, Tijana and Kostas, Stefanos and Tsouvaltzis, Pavlos and Grumet, Rebecca and Kanellis, Angelos K.",
year = "2020",
abstract = "Ascorbate oxidase (AO, EC 1.10.3.3) is a copper-containing enzyme localized at the apoplast, where it catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHA) via monodehydroascorbic acid (MDHA) intermediate. Despite it has been extensively studied, no biological roles have been definitively ascribed. To understand the role of AO in plant metabolism, fruit growth and physiology, we suppressed AO expression in melon (Cucumis melo L.) fruit. Reduction of AO activity increased AA content in melon fruit, which is the result of repression of AA oxidation and simultaneous induction of certain biosynthetic and recycling genes. As a consequence, ascorbate redox state was altered in the apoplast. Interestingly, transgenic melon fruit displayed increased ethylene production rate coincided with elevated levels of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO, EC 1.14.17.4) activity and gene expression, which might contribute to earlier ripening. Moreover, AO suppressed transgenic melon fruit exhibited a dramatic arrest in fruit growth, due to a simultaneous decrease in fruit cell size and in plasmalemma (PM) ATPase activity. All the above, support for the first time, the in vivo AO participation in the rapid fruit growth of Cucurbitaceae and further suggest an alternative route for AA increase in ripening fruit.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit",
pages = "303-291",
volume = "156",
doi = "10.1016/j.plaphy.2020.08.040"
}

Chatzopoulou, F., Sanmartin, M., Mellidou, I., Pateraki, I., Koukounaras, A., Tanou, G., Kalamaki, M. S., Veljović-Jovanović, S., Cvetić-Antić, T., Kostas, S., Tsouvaltzis, P., Grumet, R.,& Kanellis, A. K.. (2020). Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 156, 291-303.
https://doi.org/10.1016/j.plaphy.2020.08.040

Chatzopoulou F, Sanmartin M, Mellidou I, Pateraki I, Koukounaras A, Tanou G, Kalamaki MS, Veljović-Jovanović S, Cvetić-Antić T, Kostas S, Tsouvaltzis P, Grumet R, Kanellis AK. Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit. in Plant Physiology and Biochemistry. 2020;156:291-303.
doi:10.1016/j.plaphy.2020.08.040 .

Chatzopoulou, Fani, Sanmartin, Maite, Mellidou, Ifigeneia, Pateraki, Irini, Koukounaras, Athanasios, Tanou, Georgia, Kalamaki, Mary S., Veljović-Jovanović, Sonja, Cvetić-Antić, Tijana, Kostas, Stefanos, Tsouvaltzis, Pavlos, Grumet, Rebecca, Kanellis, Angelos K., "Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit" in Plant Physiology and Biochemistry, 156 (2020):291-303,
https://doi.org/10.1016/j.plaphy.2020.08.040 . .