TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Postic, Dobrivoj
AU  - Aleksic, Goran
AU  - Veljović-Jovanović, Sonja
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1208
AB  - Bacterial leaf spot caused by the plant pathogenic bacterium Pseudomonas syringae pv. coriandricola (Psc) was observed on carrot, parsnip, and parsley grown on a vegetable farm in the Vojvodina Province of Serbia. Nonfluorescent bacterial colonies were isolated from diseased leaves and characterized using different molecular techniques. Repetitive element PCR fingerprinting with five oligonucleotide primers (BOX, ERIC, GTG(5), REP, and SERE) and the randomly amplified polymorphic DNA-PCR with the M13 primer revealed identical fingerprint patterns for all tested strains. Multilocus sequence analysis of four housekeeping genes (gapA, gltA, gyrB, and rpoD) showed a high degree (99.8 to 100%) of homology with sequences of Psc strains deposited in the Plant Associated Microbes Database and NCBI database. The tested strains caused bacterial leaf spot symptoms on all three host plants. Host-strain specificity was not found in cross-pathogenicity tests, but the plant response (peroxidase induction and chlorophyll bleaching) was more pronounced in carrot and parsley than in parsnip.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip
EP  - 3082
IS  - 12
SP  - 3072
VL  - 103
DO  - 10.1094/PDIS-03-19-0674-RE
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Dimkić, Ivica and Stanković, Slaviša and Postic, Dobrivoj and Aleksic, Goran and Veljović-Jovanović, Sonja",
year = "2019",
abstract = "Bacterial leaf spot caused by the plant pathogenic bacterium Pseudomonas syringae pv. coriandricola (Psc) was observed on carrot, parsnip, and parsley grown on a vegetable farm in the Vojvodina Province of Serbia. Nonfluorescent bacterial colonies were isolated from diseased leaves and characterized using different molecular techniques. Repetitive element PCR fingerprinting with five oligonucleotide primers (BOX, ERIC, GTG(5), REP, and SERE) and the randomly amplified polymorphic DNA-PCR with the M13 primer revealed identical fingerprint patterns for all tested strains. Multilocus sequence analysis of four housekeeping genes (gapA, gltA, gyrB, and rpoD) showed a high degree (99.8 to 100%) of homology with sequences of Psc strains deposited in the Plant Associated Microbes Database and NCBI database. The tested strains caused bacterial leaf spot symptoms on all three host plants. Host-strain specificity was not found in cross-pathogenicity tests, but the plant response (peroxidase induction and chlorophyll bleaching) was more pronounced in carrot and parsley than in parsnip.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip",
pages = "3082-3072",
number = "12",
volume = "103",
doi = "10.1094/PDIS-03-19-0674-RE"
}

Popović, T., Jelušić, A., Dimkić, I., Stanković, S., Postic, D., Aleksic, G.,& Veljović-Jovanović, S.. (2019). Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(12), 3072-3082.
https://doi.org/10.1094/PDIS-03-19-0674-RE

Popović T, Jelušić A, Dimkić I, Stanković S, Postic D, Aleksic G, Veljović-Jovanović S. Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip. in Plant Disease. 2019;103(12):3072-3082.
doi:10.1094/PDIS-03-19-0674-RE .

Popović, Tatjana, Jelušić, Aleksandra, Dimkić, Ivica, Stanković, Slaviša, Postic, Dobrivoj, Aleksic, Goran, Veljović-Jovanović, Sonja, "Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip" in Plant Disease, 103, no. 12 (2019):3072-3082,
https://doi.org/10.1094/PDIS-03-19-0674-RE . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Blagojević, Jovana
AU  - Aleksic, Goran
AU  - Jelušić, Aleksandra
AU  - Krnjajić, Slobodan
AU  - Milovanović, Predrag
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1136
AB  - Unusual blight-like symptoms appeared on highbush blueberry plants in Serbia during August 2015 and infected plants showed browning and reddening of leaves, drying of foliage and brown discolouration of internal vascular stem tissues. The objective of this study was to isolate and confirm a causal agent of the disease. Five diseased blueberry plants (2-year-old), with visible brown discolouration in the wood, were collected for isolation on potato dextrose agar (PDA). Morphological analysis of the selected fungal isolates showed the presence of abundant black, round to oblong, or irregularly shaped microsclerotia immersed in the PDA. Dark, globose pycnidia formed on water agar with an initially hyaline, granular content and single-celled conidia, indicating the presence of plant pathogenic fungus Macrophomina phaseolina associated with symptomatic plant tissues. Pathogenicity was confirmed on potted blueberry plants based on the initial symptoms of leaves turning yellowish to brown at the leaf edges, followed by the defoliation of leaves of the inoculated stems. Discolouration of vascular tissues was also observed on transverse sections of inoculated stems. The pathogen M. phaseolina was confirmed using molecular analysis of the ITS1-5.8S-ITS2 region of rDNA and a part of the TEF-1 gene region. This is the first report of M. phaseolina causing a blight disease on highbush blueberry in Serbia. The study should help in elucidating disease symptomatology and provide information on the risk which this fungus could pose in blueberry production.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Canadian Journal of Plant Pathology
T1  - A blight disease on highbush blueberry associated with Macrophomina phaseolina in Serbia
EP  - 127
IS  - 1
SP  - 121
VL  - 40
DO  - 10.1080/07060661.2017.1415977
ER  - 

@article{
author = "Popović, Tatjana and Blagojević, Jovana and Aleksic, Goran and Jelušić, Aleksandra and Krnjajić, Slobodan and Milovanović, Predrag",
year = "2018",
abstract = "Unusual blight-like symptoms appeared on highbush blueberry plants in Serbia during August 2015 and infected plants showed browning and reddening of leaves, drying of foliage and brown discolouration of internal vascular stem tissues. The objective of this study was to isolate and confirm a causal agent of the disease. Five diseased blueberry plants (2-year-old), with visible brown discolouration in the wood, were collected for isolation on potato dextrose agar (PDA). Morphological analysis of the selected fungal isolates showed the presence of abundant black, round to oblong, or irregularly shaped microsclerotia immersed in the PDA. Dark, globose pycnidia formed on water agar with an initially hyaline, granular content and single-celled conidia, indicating the presence of plant pathogenic fungus Macrophomina phaseolina associated with symptomatic plant tissues. Pathogenicity was confirmed on potted blueberry plants based on the initial symptoms of leaves turning yellowish to brown at the leaf edges, followed by the defoliation of leaves of the inoculated stems. Discolouration of vascular tissues was also observed on transverse sections of inoculated stems. The pathogen M. phaseolina was confirmed using molecular analysis of the ITS1-5.8S-ITS2 region of rDNA and a part of the TEF-1 gene region. This is the first report of M. phaseolina causing a blight disease on highbush blueberry in Serbia. The study should help in elucidating disease symptomatology and provide information on the risk which this fungus could pose in blueberry production.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Canadian Journal of Plant Pathology",
title = "A blight disease on highbush blueberry associated with Macrophomina phaseolina in Serbia",
pages = "127-121",
number = "1",
volume = "40",
doi = "10.1080/07060661.2017.1415977"
}

Popović, T., Blagojević, J., Aleksic, G., Jelušić, A., Krnjajić, S.,& Milovanović, P.. (2018). A blight disease on highbush blueberry associated with Macrophomina phaseolina in Serbia. in Canadian Journal of Plant Pathology
Taylor & Francis Ltd, Abingdon., 40(1), 121-127.
https://doi.org/10.1080/07060661.2017.1415977

Popović T, Blagojević J, Aleksic G, Jelušić A, Krnjajić S, Milovanović P. A blight disease on highbush blueberry associated with Macrophomina phaseolina in Serbia. in Canadian Journal of Plant Pathology. 2018;40(1):121-127.
doi:10.1080/07060661.2017.1415977 .

Popović, Tatjana, Blagojević, Jovana, Aleksic, Goran, Jelušić, Aleksandra, Krnjajić, Slobodan, Milovanović, Predrag, "A blight disease on highbush blueberry associated with Macrophomina phaseolina in Serbia" in Canadian Journal of Plant Pathology, 40, no. 1 (2018):121-127,
https://doi.org/10.1080/07060661.2017.1415977 . .