TY  - JOUR
AU  - Marković, Sanja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Ilicic, Renata
AU  - Bagi, Ferenc
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1542
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia
IS  - 1
VL  - 106
DO  - 10.1094/PDIS-06-21-1128-PDN
ER  - 

@article{
author = "Marković, Sanja and Milić Komić, Sonja and Jelušić, Aleksandra and Ilicic, Renata and Bagi, Ferenc and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia",
number = "1",
volume = "106",
doi = "10.1094/PDIS-06-21-1128-PDN"
}

Marković, S., Milić Komić, S., Jelušić, A., Ilicic, R., Bagi, F., Stanković, S.,& Popović, T.. (2022). First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 106(1).
https://doi.org/10.1094/PDIS-06-21-1128-PDN

Marković S, Milić Komić S, Jelušić A, Ilicic R, Bagi F, Stanković S, Popović T. First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease. 2022;106(1).
doi:10.1094/PDIS-06-21-1128-PDN .

Marković, Sanja, Milić Komić, Sonja, Jelušić, Aleksandra, Ilicic, Renata, Bagi, Ferenc, Stanković, Slaviša, Popović, Tatjana, "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia" in Plant Disease, 106, no. 1 (2022),
https://doi.org/10.1094/PDIS-06-21-1128-PDN . .

TY  - JOUR
AU  - Ilicic, Renata
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Barac, Goran
AU  - Bagi, Ferenc
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1531
AB  - In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Sancevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes-gapA, gltA, rpoD and gyrB-showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305(T) (=58(T) = LMG28609(T)) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +- - - + (Pseudomonas Group Ia) and GATTa + - + -, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification
EP  - 150
IS  - 1
SP  - 140
VL  - 180
DO  - 10.1111/aab.12717
ER  - 

@article{
author = "Ilicic, Renata and Jelušić, Aleksandra and Marković, Sanja and Barac, Goran and Bagi, Ferenc and Popović, Tatjana",
year = "2022",
abstract = "In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Sancevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes-gapA, gltA, rpoD and gyrB-showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305(T) (=58(T) = LMG28609(T)) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +- - - + (Pseudomonas Group Ia) and GATTa + - + -, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification",
pages = "150-140",
number = "1",
volume = "180",
doi = "10.1111/aab.12717"
}

Ilicic, R., Jelušić, A., Marković, S., Barac, G., Bagi, F.,& Popović, T.. (2022). Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology
Wiley, Hoboken., 180(1), 140-150.
https://doi.org/10.1111/aab.12717

Ilicic R, Jelušić A, Marković S, Barac G, Bagi F, Popović T. Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology. 2022;180(1):140-150.
doi:10.1111/aab.12717 .

Ilicic, Renata, Jelušić, Aleksandra, Marković, Sanja, Barac, Goran, Bagi, Ferenc, Popović, Tatjana, "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification" in Annals of Applied Biology, 180, no. 1 (2022):140-150,
https://doi.org/10.1111/aab.12717 . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Ilicic, Renata
AU  - Veljović-Jovanović, Sonja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1438
AB  - Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013-2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2-22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3-5 were highly intensified in response to R. solanacearum.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers
EP  - 1959
IS  - 8
SP  - 1945
VL  - 70
DO  - 10.1111/ppa.13421
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Ilicic, Renata and Veljović-Jovanović, Sonja and Milić Komić, Sonja and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013-2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2-22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3-5 were highly intensified in response to R. solanacearum.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers",
pages = "1959-1945",
number = "8",
volume = "70",
doi = "10.1111/ppa.13421"
}

Marković, S., Stanković, S., Ilicic, R., Veljović-Jovanović, S., Milić Komić, S., Jelušić, A.,& Popović, T.. (2021). Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology
Wiley, Hoboken., 70(8), 1945-1959.
https://doi.org/10.1111/ppa.13421

Marković S, Stanković S, Ilicic R, Veljović-Jovanović S, Milić Komić S, Jelušić A, Popović T. Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology. 2021;70(8):1945-1959.
doi:10.1111/ppa.13421 .

Marković, Sanja, Stanković, Slaviša, Ilicic, Renata, Veljović-Jovanović, Sonja, Milić Komić, Sonja, Jelušić, Aleksandra, Popović, Tatjana, "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers" in Plant Pathology, 70, no. 8 (2021):1945-1959,
https://doi.org/10.1111/ppa.13421 . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Ilicic, Renata
AU  - Kosovac, Andrea
AU  - Postic, Dobrivoj
AU  - Popović, Tatjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1494
AB  - Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Backa region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out of 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent of potato blackleg in Serbia for the first time (29 isolates). Dickeya dianthicola was a less frequently causative bacterium, which was also noticed for the first time (nine isolates). A total of 38 isolates were characterized based on their phenotypic and genetic features, including a pathogenicity test on potato. The repetitive element PCR (rep-PCR) using BOX, REP, and ERIC primer pairs differentiated five genetic profiles among 38 tested isolates. Multilocus sequence analysis (MLSA) of four housekeeping genes, acnA, gapA, icdA, and mdh, revealed the presence of three so far unknown P. c. subsp. brasiliensis multilocus genotypes and confirmed clustering into two main genetic clades as determined in other studies. MLSA also revealed the presence of a new genotype of D. dianthicola in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia
EP  - 1090
IS  - 4
SP  - 1080
VL  - 105
DO  - 10.1094/PDIS-05-20-1076-RE
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Ilicic, Renata and Kosovac, Andrea and Postic, Dobrivoj and Popović, Tatjana",
year = "2021",
abstract = "Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Backa region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out of 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent of potato blackleg in Serbia for the first time (29 isolates). Dickeya dianthicola was a less frequently causative bacterium, which was also noticed for the first time (nine isolates). A total of 38 isolates were characterized based on their phenotypic and genetic features, including a pathogenicity test on potato. The repetitive element PCR (rep-PCR) using BOX, REP, and ERIC primer pairs differentiated five genetic profiles among 38 tested isolates. Multilocus sequence analysis (MLSA) of four housekeeping genes, acnA, gapA, icdA, and mdh, revealed the presence of three so far unknown P. c. subsp. brasiliensis multilocus genotypes and confirmed clustering into two main genetic clades as determined in other studies. MLSA also revealed the presence of a new genotype of D. dianthicola in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia",
pages = "1090-1080",
number = "4",
volume = "105",
doi = "10.1094/PDIS-05-20-1076-RE"
}

Marković, S., Stanković, S., Jelušić, A., Ilicic, R., Kosovac, A., Postic, D.,& Popović, T.. (2021). Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 105(4), 1080-1090.
https://doi.org/10.1094/PDIS-05-20-1076-RE

Marković S, Stanković S, Jelušić A, Ilicic R, Kosovac A, Postic D, Popović T. Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia. in Plant Disease. 2021;105(4):1080-1090.
doi:10.1094/PDIS-05-20-1076-RE .

Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Ilicic, Renata, Kosovac, Andrea, Postic, Dobrivoj, Popović, Tatjana, "Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in Some Potato Fields in Serbia" in Plant Disease, 105, no. 4 (2021):1080-1090,
https://doi.org/10.1094/PDIS-05-20-1076-RE . .

TY  - JOUR
AU  - Ilicic, Renata
AU  - Popović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Bagi, Ferenc
AU  - Vlajic, Slobodan
AU  - Stanković, Slaviša
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1419
AB  - Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia
EP  - 262
IS  - 1
SP  - 247
VL  - 53
DO  - 10.2298/GENSR2101247I
ER  - 

@article{
author = "Ilicic, Renata and Popović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Bagi, Ferenc and Vlajic, Slobodan and Stanković, Slaviša",
year = "2021",
abstract = "Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia",
pages = "262-247",
number = "1",
volume = "53",
doi = "10.2298/GENSR2101247I"
}

Ilicic, R., Popović, T., Marković, S., Jelušić, A., Bagi, F., Vlajic, S.,& Stanković, S.. (2021). Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 53(1), 247-262.
https://doi.org/10.2298/GENSR2101247I

Ilicic R, Popović T, Marković S, Jelušić A, Bagi F, Vlajic S, Stanković S. Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika-Belgrade. 2021;53(1):247-262.
doi:10.2298/GENSR2101247I .

Ilicic, Renata, Popović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Bagi, Ferenc, Vlajic, Slobodan, Stanković, Slaviša, "Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia" in Genetika-Belgrade, 53, no. 1 (2021):247-262,
https://doi.org/10.2298/GENSR2101247I . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Živković, Ljiljana
AU  - Živković, Natalija
AU  - Ilicic, Renata
AU  - Stanisavljević, Rade
AU  - Stanković, Slaviša
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1350
AB  - Fire blight disease, caused by plant pathogenic bacteriaErwinia amylovorarepresents a global threat for pome fruit industry, affecting yield and causing serious economic damage. During six year period (2011-2016), a total of 45 isolates typical forE. amylovorawere isolated from four different host plants (apple, pear, quince and medlar) in 21 localities within four Serbian regions (Southeast, Central, West, and North). Isolates were primarily identified using biochemical and serological tests, polymerase chain reaction (PCR) with species-specific primer pair (A/B) and their pathogenicity. Polyclonal antibodies used in DAS ELISA and IF were found to be specific and sensitive enough for rapidE. amylovoradetection. In virulence test performed on immature pear fruitlets, higher variability (CV) was found between regions than among hosts from which isolates originated. Slighter virulence of isolates originated from North region of Serbia (Vojvodina) was found in comparison to the isolates from Central Serbia, which were the most variable. Principal components analysis (PCA) segregated quince as a host among the most prevalent isolates in group of highly virulent, but failed to identify difference related to geographic origin of Serbian isolates. The same results enabled multivariate statistical analysis. Different DNA fingerprinting techniques such as repetitive element sequence based PCR (rep-) with BOX, ERIC, REP, GTG(5)and SERE primers, then Random Amplified Polymorphic DNA (RAPD) with M13-PCR primer revealed genetic homogeneity among all SerbianE. amylovoraisolates. ERIC, SERE and M13 primers generated the most complex and visually perceptible patters. Restriction fragment length polymorphism analysis (RFLP) ofgyrAandrecAgenes, digested with three restriction endonucleasesBstUI,BgIII andBsmI andrpoSgene digested withBstUI gave the unuque, characteristic patterns for all Serbian isolates.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates
EP  - 872
IS  - 4
SP  - 857
VL  - 157
DO  - 10.1007/s10658-020-02046-1
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Živković, Ljiljana and Živković, Natalija and Ilicic, Renata and Stanisavljević, Rade and Stanković, Slaviša",
year = "2020",
abstract = "Fire blight disease, caused by plant pathogenic bacteriaErwinia amylovorarepresents a global threat for pome fruit industry, affecting yield and causing serious economic damage. During six year period (2011-2016), a total of 45 isolates typical forE. amylovorawere isolated from four different host plants (apple, pear, quince and medlar) in 21 localities within four Serbian regions (Southeast, Central, West, and North). Isolates were primarily identified using biochemical and serological tests, polymerase chain reaction (PCR) with species-specific primer pair (A/B) and their pathogenicity. Polyclonal antibodies used in DAS ELISA and IF were found to be specific and sensitive enough for rapidE. amylovoradetection. In virulence test performed on immature pear fruitlets, higher variability (CV) was found between regions than among hosts from which isolates originated. Slighter virulence of isolates originated from North region of Serbia (Vojvodina) was found in comparison to the isolates from Central Serbia, which were the most variable. Principal components analysis (PCA) segregated quince as a host among the most prevalent isolates in group of highly virulent, but failed to identify difference related to geographic origin of Serbian isolates. The same results enabled multivariate statistical analysis. Different DNA fingerprinting techniques such as repetitive element sequence based PCR (rep-) with BOX, ERIC, REP, GTG(5)and SERE primers, then Random Amplified Polymorphic DNA (RAPD) with M13-PCR primer revealed genetic homogeneity among all SerbianE. amylovoraisolates. ERIC, SERE and M13 primers generated the most complex and visually perceptible patters. Restriction fragment length polymorphism analysis (RFLP) ofgyrAandrecAgenes, digested with three restriction endonucleasesBstUI,BgIII andBsmI andrpoSgene digested withBstUI gave the unuque, characteristic patterns for all Serbian isolates.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates",
pages = "872-857",
number = "4",
volume = "157",
doi = "10.1007/s10658-020-02046-1"
}

Popović, T., Jelušić, A., Živković, L., Živković, N., Ilicic, R., Stanisavljević, R.,& Stanković, S.. (2020). Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates. in European Journal of Plant Pathology
Springer, Dordrecht., 157(4), 857-872.
https://doi.org/10.1007/s10658-020-02046-1

Popović T, Jelušić A, Živković L, Živković N, Ilicic R, Stanisavljević R, Stanković S. Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates. in European Journal of Plant Pathology. 2020;157(4):857-872.
doi:10.1007/s10658-020-02046-1 .

Popović, Tatjana, Jelušić, Aleksandra, Živković, Ljiljana, Živković, Natalija, Ilicic, Renata, Stanisavljević, Rade, Stanković, Slaviša, "Identification, genetic characterization and virulence of SerbianErwinia amylovoraisolates" in European Journal of Plant Pathology, 157, no. 4 (2020):857-872,
https://doi.org/10.1007/s10658-020-02046-1 . .