TY  - CONF
AU  - Savic, Danijela
AU  - Opačić, Miloš
AU  - Nestorov, Jelena
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Bascarevic, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Zorović, Maja
AU  - Živin, Marko
AU  - Selih, Vid Simon
AU  - Spasic, Snezana
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2841
AB  - More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques, optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals, we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal to pathology of mTLE or they represent epiphenomenon.
PB  - FENS
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - The importance of copper in pathology of mesial temporal lobe epilepsy
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2841
ER  - 

@conference{
author = "Savic, Danijela and Opačić, Miloš and Nestorov, Jelena and Ristic, Aleksandar J and Sokic, Dragoslav and Bascarevic, Vladimir and Raičević, Savo and Savić, Slobodan and Zorović, Maja and Živin, Marko and Selih, Vid Simon and Spasic, Snezana and Spasojević, Ivan",
year = "2019",
abstract = "More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques, optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals, we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal to pathology of mTLE or they represent epiphenomenon.",
publisher = "FENS",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "The importance of copper in pathology of mesial temporal lobe epilepsy",
pages = "46",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2841"
}

Savic, D., Opačić, M., Nestorov, J., Ristic, A. J., Sokic, D., Bascarevic, V., Raičević, S., Savić, S., Zorović, M., Živin, M., Selih, V. S., Spasic, S.,& Spasojević, I.. (2019). The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
FENS., 46.
https://hdl.handle.net/21.15107/rcub_rimsi_2841

Savic D, Opačić M, Nestorov J, Ristic AJ, Sokic D, Bascarevic V, Raičević S, Savić S, Zorović M, Živin M, Selih VS, Spasic S, Spasojević I. The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:46.
https://hdl.handle.net/21.15107/rcub_rimsi_2841 .

Savic, Danijela, Opačić, Miloš, Nestorov, Jelena, Ristic, Aleksandar J, Sokic, Dragoslav, Bascarevic, Vladimir, Raičević, Savo, Savić, Slobodan, Zorović, Maja, Živin, Marko, Selih, Vid Simon, Spasic, Snezana, Spasojević, Ivan, "The importance of copper in pathology of mesial temporal lobe epilepsy" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):46,
https://hdl.handle.net/21.15107/rcub_rimsi_2841 .

TY  - CONF
AU  - Opačić, Miloš
AU  - Nestorov, Jelena
AU  - Tasić, Danica
AU  - Bogdanović Pristov, Jelena
AU  - Savić, Danijela Z
AU  - Bascarevic, Vladimir
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2810
AB  - Skleroza hipokampusa je najčešći neuropatološki nalaz kod epilepsije temporalnog
režnja. Pored morfoloških promena odlikuju ga i promene nivoa različitih proteina u
ćeliji. Imunoblot je nezamenjiva metoda za određivanje nivoa proteina i podrazumeva
normalizaciju u odnosu na endogenu kontrolu (kontrolu jednakog nanošenja uzorka). Za
tu svrhu se koriste proteini za koje se smatra da imaju stabilnu ekspresiju koja najmanje
varira u ispitivanim uslovima, tretmanima i patofiziološkim stanjima. Ipak u nekim
patofiziološkim stanjima se i ekspresija tradicionalno korišćenih endogenih kontrola
menja,
1
što može dovesti do prikupljanja netačnih podataka i pogrešnog tumačenja
rezultata. Cilj ovog rada je bio da se ispita stabilnost najčešće korišćenih endogenih
kontrola i izabere najstabilnija koja će se koristiti za normalizaciju pri poređenju nivoa
ciljnih proteina hipokampusa obolelih od epilepsije i neurološki intaktnih kontrola.
Imunoblotom su detektovani β-aktin, α-tubulin, TATA-vezujući protein i gliceraldehid3-fosfat dehidrogenaza u tkivnim ekstraktima hipokampusa 9 pacijenata i 7 kontrola, i
NormFinder softverom2
određene unutargrupna i međugrupna varijabilnost. Pokazano je
da nivo strukturnih proteina β-aktina i α-tubulina najmanje varira u ispitivanim uslovima
te se mogu koristiti za poređenje nivoa proteina u sklerotičnim i intaktnim
hipokampusima. Rezultati upućuju da je odabir endogenih kontrola važan preduslov za
dobijanje tačnih i pouzdanih podataka imunoblot metodom.
PB  - Društvo biologa Srbije
C3  - Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave
T1  - Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja
SP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2810
ER  - 

@conference{
author = "Opačić, Miloš and Nestorov, Jelena and Tasić, Danica and Bogdanović Pristov, Jelena and Savić, Danijela Z and Bascarevic, Vladimir and Ristic, Aleksandar J and Sokic, Dragoslav and Spasojević, Ivan",
year = "2018",
abstract = "Skleroza hipokampusa je najčešći neuropatološki nalaz kod epilepsije temporalnog
režnja. Pored morfoloških promena odlikuju ga i promene nivoa različitih proteina u
ćeliji. Imunoblot je nezamenjiva metoda za određivanje nivoa proteina i podrazumeva
normalizaciju u odnosu na endogenu kontrolu (kontrolu jednakog nanošenja uzorka). Za
tu svrhu se koriste proteini za koje se smatra da imaju stabilnu ekspresiju koja najmanje
varira u ispitivanim uslovima, tretmanima i patofiziološkim stanjima. Ipak u nekim
patofiziološkim stanjima se i ekspresija tradicionalno korišćenih endogenih kontrola
menja,
1
što može dovesti do prikupljanja netačnih podataka i pogrešnog tumačenja
rezultata. Cilj ovog rada je bio da se ispita stabilnost najčešće korišćenih endogenih
kontrola i izabere najstabilnija koja će se koristiti za normalizaciju pri poređenju nivoa
ciljnih proteina hipokampusa obolelih od epilepsije i neurološki intaktnih kontrola.
Imunoblotom su detektovani β-aktin, α-tubulin, TATA-vezujući protein i gliceraldehid3-fosfat dehidrogenaza u tkivnim ekstraktima hipokampusa 9 pacijenata i 7 kontrola, i
NormFinder softverom2
određene unutargrupna i međugrupna varijabilnost. Pokazano je
da nivo strukturnih proteina β-aktina i α-tubulina najmanje varira u ispitivanim uslovima
te se mogu koristiti za poređenje nivoa proteina u sklerotičnim i intaktnim
hipokampusima. Rezultati upućuju da je odabir endogenih kontrola važan preduslov za
dobijanje tačnih i pouzdanih podataka imunoblot metodom.",
publisher = "Društvo biologa Srbije",
journal = "Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave",
title = "Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja",
pages = "164",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2810"
}

Opačić, M., Nestorov, J., Tasić, D., Bogdanović Pristov, J., Savić, D. Z., Bascarevic, V., Ristic, A. J., Sokic, D.,& Spasojević, I.. (2018). Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja. in Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave
Društvo biologa Srbije., 164.
https://hdl.handle.net/21.15107/rcub_rimsi_2810

Opačić M, Nestorov J, Tasić D, Bogdanović Pristov J, Savić DZ, Bascarevic V, Ristic AJ, Sokic D, Spasojević I. Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja. in Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave. 2018;:164.
https://hdl.handle.net/21.15107/rcub_rimsi_2810 .

Opačić, Miloš, Nestorov, Jelena, Tasić, Danica, Bogdanović Pristov, Jelena, Savić, Danijela Z, Bascarevic, Vladimir, Ristic, Aleksandar J, Sokic, Dragoslav, Spasojević, Ivan, "Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja" in Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave (2018):164,
https://hdl.handle.net/21.15107/rcub_rimsi_2810 .

TY  - JOUR
AU  - Ristic, Aleksandar J
AU  - Savić, Danijela Z
AU  - Sokic, Dragoslav
AU  - Bogdanović Pristov, Jelena
AU  - Nestorov, Jelena
AU  - Baščarević, Vladimir
AU  - Raicević, Savo
AU  - Savić, Slobodan
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/898
AB  - ObjectiveTo examine antioxidative system in hippocampi of patients with mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). MethodsActivity and levels of antioxidative enzymescatalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), manganese superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS patients (mean age 37.7[standard deviation] 6.6years) who underwent amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from five neurologically intact controls (mean age 34.49.0years). Subfield and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was analyzed in detail using immunohistochemical staining. ResultsSclerotic hippocampi showed drastically increased activity of hydrogen peroxide-removing enzymes, CAT (p lt 0.001), GPx (p lt 0.001), and GR (p lt 0.001), and significantly higher protein levels of CAT (p=0.006), GPx (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT immunofluorescence was located mainly in neurons in both controls and HS. Control hippocampi showed GPx staining in blood vessels and CA neurons. In HS, GPx-rich loci, representing bundles of astrocytes, emerged in different hippocampal regions, whereas the number of GPx-positive vessels was drastically decreased. Neurons with abnormal morphology and strong MnSOD immunofluorescence were present in all neuronal layers in HS. Small autofluorescent deposits, most likely lipofuscin, were observed, along with astrogliosis, in CA1 in HS. SignificanceAntioxidative system is upregulated in HS. This documents, for the first time, that epileptogenic hippocampi are exposed to oxidative stress. Our findings provide a basis for understanding the potential involvement of redox alterations in the pathology of epilepsy, and may open new pharmacologic perspectives for mTLE-HS treatment.
PB  - Wiley, Hoboken
T2  - Epilepsia
T1  - Hippocampal antioxidative system in mesial temporal lobe epilepsy
EP  - 799
IS  - 5
SP  - 789
VL  - 56
DO  - 10.1111/epi.12981
ER  - 

@article{
author = "Ristic, Aleksandar J and Savić, Danijela Z and Sokic, Dragoslav and Bogdanović Pristov, Jelena and Nestorov, Jelena and Baščarević, Vladimir and Raicević, Savo and Savić, Slobodan and Spasojević, Ivan",
year = "2015",
abstract = "ObjectiveTo examine antioxidative system in hippocampi of patients with mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). MethodsActivity and levels of antioxidative enzymescatalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), manganese superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS patients (mean age 37.7[standard deviation] 6.6years) who underwent amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from five neurologically intact controls (mean age 34.49.0years). Subfield and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was analyzed in detail using immunohistochemical staining. ResultsSclerotic hippocampi showed drastically increased activity of hydrogen peroxide-removing enzymes, CAT (p lt 0.001), GPx (p lt 0.001), and GR (p lt 0.001), and significantly higher protein levels of CAT (p=0.006), GPx (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT immunofluorescence was located mainly in neurons in both controls and HS. Control hippocampi showed GPx staining in blood vessels and CA neurons. In HS, GPx-rich loci, representing bundles of astrocytes, emerged in different hippocampal regions, whereas the number of GPx-positive vessels was drastically decreased. Neurons with abnormal morphology and strong MnSOD immunofluorescence were present in all neuronal layers in HS. Small autofluorescent deposits, most likely lipofuscin, were observed, along with astrogliosis, in CA1 in HS. SignificanceAntioxidative system is upregulated in HS. This documents, for the first time, that epileptogenic hippocampi are exposed to oxidative stress. Our findings provide a basis for understanding the potential involvement of redox alterations in the pathology of epilepsy, and may open new pharmacologic perspectives for mTLE-HS treatment.",
publisher = "Wiley, Hoboken",
journal = "Epilepsia",
title = "Hippocampal antioxidative system in mesial temporal lobe epilepsy",
pages = "799-789",
number = "5",
volume = "56",
doi = "10.1111/epi.12981"
}

Ristic, A. J., Savić, D. Z., Sokic, D., Bogdanović Pristov, J., Nestorov, J., Baščarević, V., Raicević, S., Savić, S.,& Spasojević, I.. (2015). Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia
Wiley, Hoboken., 56(5), 789-799.
https://doi.org/10.1111/epi.12981

Ristic AJ, Savić DZ, Sokic D, Bogdanović Pristov J, Nestorov J, Baščarević V, Raicević S, Savić S, Spasojević I. Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia. 2015;56(5):789-799.
doi:10.1111/epi.12981 .

Ristic, Aleksandar J, Savić, Danijela Z, Sokic, Dragoslav, Bogdanović Pristov, Jelena, Nestorov, Jelena, Baščarević, Vladimir, Raicević, Savo, Savić, Slobodan, Spasojević, Ivan, "Hippocampal antioxidative system in mesial temporal lobe epilepsy" in Epilepsia, 56, no. 5 (2015):789-799,
https://doi.org/10.1111/epi.12981 . .