TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kilsey
AU  - Oswald, Petar
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2044
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
EP  - 32
IS  - 1
SP  - 21
VL  - 38
DO  - 10.1093/mutage/geac024
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kilsey and Oswald, Petar and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
pages = "32-21",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024"
}

Jovanović Marić, J., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević Aleksić J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kilsey, Oswald, Petar, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - JOUR
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Oalđe Pavlović, Mariana
AU  - Sladić, Dušan
AU  - Novaković, Irena
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2018
AB  - Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.
PB  - National Library of Serbia
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone
EP  - 1258
IS  - 11
SP  - 1245
VL  - 87
DO  - 10.2298/JSC220304044D
ER  - 

@article{
author = "Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Oalđe Pavlović, Mariana and Sladić, Dušan and Novaković, Irena and Vuković-Gačić, Branka",
year = "2022",
abstract = "Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.",
publisher = "National Library of Serbia",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone",
pages = "1258-1245",
number = "11",
volume = "87",
doi = "10.2298/JSC220304044D"
}

Đorđević Aleksić, J., Kolarević, S., Jovanović Marić, J., Oalđe Pavlović, M., Sladić, D., Novaković, I.,& Vuković-Gačić, B.. (2022). Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society
National Library of Serbia., 87(11), 1245-1258.
https://doi.org/10.2298/JSC220304044D

Đorđević Aleksić J, Kolarević S, Jovanović Marić J, Oalđe Pavlović M, Sladić D, Novaković I, Vuković-Gačić B. Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society. 2022;87(11):1245-1258.
doi:10.2298/JSC220304044D .

Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Oalđe Pavlović, Mariana, Sladić, Dušan, Novaković, Irena, Vuković-Gačić, Branka, "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone" in Journal of the Serbian Chemical Society, 87, no. 11 (2022):1245-1258,
https://doi.org/10.2298/JSC220304044D . .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
AU  - Simonovic, Predrag
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1913
AB  - One of the major limitations in performing ecogenotoxicological studies is the distance between research field and the laboratory. As some of the methods used in ecogenotoxicology require fresh biological material with intact cell viability, transfer of samples to the laboratory within a few hours after sampling is usually required. To overcome this issue, we have introduced cryopreservation in our research as a possible solution. Cryopreservation is a method which includes preservation of intact, living cells at low temperature for a long time. In natural conditions freezing, forming of ice crystals and dehydration could destroy cell structures. To avoid this consequence, specific compounds were introduced, cryoprotective agents, in the method of cryopreservation. The main characteristic of these compounds is their ability to reduce ice crystal formation in cells at any temperature.
We have applied cryopreservation in the evaluation of genotoxic potential along different river streams (the Adige River, the Sava River and the Velika Morava River basin). For this purpose, we focused on the level of DNA damage of cryopreserved fish blood cells (Salmo cenerinus, Salmo marmoratus, Alburnus alburnus) by using the comet assay.
To test whether cryopreservation has the impact on cell viability, or that it induces additional DNA damage, we employed preliminary experiments in 4 Abramis brama and 8 A. alburnus specimens. Namely, from every specimen two blood samples were taken: one for analyzing cells viability and the level of DNA damage of fresh blood, and another for observing cell viability and DNA damage of cryopreserved samples. The viability of cell blood was determined by using acridine orange/ethidium bromide differential staining. For analyzing the level of DNA damage alkaline comet assay was used. Obtained results indicated that cryopreserved blood cells had approximately the same viability and the level of DNA damage as nonpreserved blood samples.
According to our results, cryopreservation is a very useful method in genotoxicology and could have many benefits: blood samples should not be analyzed immediately after sampling; samples could be transported in liquid nitrogen without concern about additional DNA damage.
PB  - University of Agriculture in Krakow
C3  - Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
T1  - Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1913
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Đorđević Aleksić, Jelena and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Kostić-Vuković, Jovana and Sunjog, Karolina and Paunović, Momir and Simonovic, Predrag and Vuković-Gačić, Branka",
year = "2018",
abstract = "One of the major limitations in performing ecogenotoxicological studies is the distance between research field and the laboratory. As some of the methods used in ecogenotoxicology require fresh biological material with intact cell viability, transfer of samples to the laboratory within a few hours after sampling is usually required. To overcome this issue, we have introduced cryopreservation in our research as a possible solution. Cryopreservation is a method which includes preservation of intact, living cells at low temperature for a long time. In natural conditions freezing, forming of ice crystals and dehydration could destroy cell structures. To avoid this consequence, specific compounds were introduced, cryoprotective agents, in the method of cryopreservation. The main characteristic of these compounds is their ability to reduce ice crystal formation in cells at any temperature.
We have applied cryopreservation in the evaluation of genotoxic potential along different river streams (the Adige River, the Sava River and the Velika Morava River basin). For this purpose, we focused on the level of DNA damage of cryopreserved fish blood cells (Salmo cenerinus, Salmo marmoratus, Alburnus alburnus) by using the comet assay.
To test whether cryopreservation has the impact on cell viability, or that it induces additional DNA damage, we employed preliminary experiments in 4 Abramis brama and 8 A. alburnus specimens. Namely, from every specimen two blood samples were taken: one for analyzing cells viability and the level of DNA damage of fresh blood, and another for observing cell viability and DNA damage of cryopreserved samples. The viability of cell blood was determined by using acridine orange/ethidium bromide differential staining. For analyzing the level of DNA damage alkaline comet assay was used. Obtained results indicated that cryopreserved blood cells had approximately the same viability and the level of DNA damage as nonpreserved blood samples.
According to our results, cryopreservation is a very useful method in genotoxicology and could have many benefits: blood samples should not be analyzed immediately after sampling; samples could be transported in liquid nitrogen without concern about additional DNA damage.",
publisher = "University of Agriculture in Krakow",
journal = "Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland",
title = "Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems",
pages = "134",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1913"
}

Jovanović Marić, J., Đorđević Aleksić, J., Kolarević, S., Kračun-Kolarević, M., Kostić-Vuković, J., Sunjog, K., Paunović, M., Simonovic, P.,& Vuković-Gačić, B.. (2018). Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
University of Agriculture in Krakow., 134.
https://hdl.handle.net/21.15107/rcub_rimsi_1913

Jovanović Marić J, Đorđević Aleksić J, Kolarević S, Kračun-Kolarević M, Kostić-Vuković J, Sunjog K, Paunović M, Simonovic P, Vuković-Gačić B. Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland. 2018;:134.
https://hdl.handle.net/21.15107/rcub_rimsi_1913 .

Jovanović Marić, Jovana, Đorđević Aleksić, Jelena, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Kostić-Vuković, Jovana, Sunjog, Karolina, Paunović, Momir, Simonovic, Predrag, Vuković-Gačić, Branka, "Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems" in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland (2018):134,
https://hdl.handle.net/21.15107/rcub_rimsi_1913 .