TY  - JOUR
AU  - Oalde, Mariana
AU  - Kolarević, Stoimir
AU  - Živković, Jelena
AU  - Alimpic-Aradski, Ana
AU  - Jovanović-Marić, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Marin, Petar D.
AU  - Savikin, Katarina
AU  - Vukovic-Gacic, Branka
AU  - Duletic-Lausević, Sonja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1454
AB  - This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g(-1)), naringin (up to 5.07 mg g(-1)) and luteolin-7-O-glucoside (up to 3.54 mg g(-1)). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro
EP  - 3245
IS  - 7
SP  - 3233
VL  - 12
DO  - 10.1039/d1fo00447f
ER  - 

@article{
author = "Oalde, Mariana and Kolarević, Stoimir and Živković, Jelena and Alimpic-Aradski, Ana and Jovanović-Marić, Jovana and Kracun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Marin, Petar D. and Savikin, Katarina and Vukovic-Gacic, Branka and Duletic-Lausević, Sonja",
year = "2021",
abstract = "This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g(-1)), naringin (up to 5.07 mg g(-1)) and luteolin-7-O-glucoside (up to 3.54 mg g(-1)). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro",
pages = "3245-3233",
number = "7",
volume = "12",
doi = "10.1039/d1fo00447f"
}

Oalde, M., Kolarević, S., Živković, J., Alimpic-Aradski, A., Jovanović-Marić, J., Kracun-Kolarević, M., Đorđević Aleksić, J., Marin, P. D., Savikin, K., Vukovic-Gacic, B.,& Duletic-Lausević, S.. (2021). A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function
Royal Soc Chemistry, Cambridge., 12(7), 3233-3245.
https://doi.org/10.1039/d1fo00447f

Oalde M, Kolarević S, Živković J, Alimpic-Aradski A, Jovanović-Marić J, Kracun-Kolarević M, Đorđević Aleksić J, Marin PD, Savikin K, Vukovic-Gacic B, Duletic-Lausević S. A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function. 2021;12(7):3233-3245.
doi:10.1039/d1fo00447f .

Oalde, Mariana, Kolarević, Stoimir, Živković, Jelena, Alimpic-Aradski, Ana, Jovanović-Marić, Jovana, Kracun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Marin, Petar D., Savikin, Katarina, Vukovic-Gacic, Branka, Duletic-Lausević, Sonja, "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro" in Food & Function, 12, no. 7 (2021):3233-3245,
https://doi.org/10.1039/d1fo00447f . .

TY  - JOUR
AU  - Pavlović, Mariana Oalde
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Jovanović-Marić, Jovana
AU  - Lunic, Tanja
AU  - Mandic, Marija
AU  - Kracun-Kolarević, Margareta
AU  - Živković, Jelena
AU  - Alimpic-Aradski, Ana
AU  - Marin, Petar D.
AU  - Savikin, Katarina
AU  - Vukovic-Gacic, Branka
AU  - Bozić-Nedeljković, Biljana
AU  - Duletic-Lausević, Sonja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1502
AB  - This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha x piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
IS  - 11
VL  - 10
DO  - 10.3390/plants10112306
ER  - 

@article{
author = "Pavlović, Mariana Oalde and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Jovanović-Marić, Jovana and Lunic, Tanja and Mandic, Marija and Kracun-Kolarević, Margareta and Živković, Jelena and Alimpic-Aradski, Ana and Marin, Petar D. and Savikin, Katarina and Vukovic-Gacic, Branka and Bozić-Nedeljković, Biljana and Duletic-Lausević, Sonja",
year = "2021",
abstract = "This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha x piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species",
number = "11",
volume = "10",
doi = "10.3390/plants10112306"
}

Pavlović, M. O., Kolarević, S., Đorđević Aleksić, J., Jovanović-Marić, J., Lunic, T., Mandic, M., Kracun-Kolarević, M., Živković, J., Alimpic-Aradski, A., Marin, P. D., Savikin, K., Vukovic-Gacic, B., Bozić-Nedeljković, B.,& Duletic-Lausević, S.. (2021). A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants-Basel
MDPI, Basel., 10(11).
https://doi.org/10.3390/plants10112306

Pavlović MO, Kolarević S, Đorđević Aleksić J, Jovanović-Marić J, Lunic T, Mandic M, Kracun-Kolarević M, Živković J, Alimpic-Aradski A, Marin PD, Savikin K, Vukovic-Gacic B, Bozić-Nedeljković B, Duletic-Lausević S. A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants-Basel. 2021;10(11).
doi:10.3390/plants10112306 .

Pavlović, Mariana Oalde, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Jovanović-Marić, Jovana, Lunic, Tanja, Mandic, Marija, Kracun-Kolarević, Margareta, Živković, Jelena, Alimpic-Aradski, Ana, Marin, Petar D., Savikin, Katarina, Vukovic-Gacic, Branka, Bozić-Nedeljković, Biljana, Duletic-Lausević, Sonja, "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species" in Plants-Basel, 10, no. 11 (2021),
https://doi.org/10.3390/plants10112306 . .