TY  - JOUR
AU  - Janković, Tamara
AU  - Goč, Sanja
AU  - Mitic, Ninoslav
AU  - Danilović Luković, Jelena
AU  - Janković, Miroslava
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2454
AB  - Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs)
whose composition is, additionally, influenced by different physiological conditions. Aiming at the
molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction
factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference
could upgrade standardization of EVs isolated by different methods and intended for use in
biomedicine.
Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic
(sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
Prostasomes recovered in LAC-separated fractions were characterized according to the distribution
of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin
CD63, and total protein/glycoprotein composition.
Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing
in GGT activity and surface glycans were separated on the WGA column. Additional populations having
distinct profiles of total glycoproteins and which can be tracked down by ALP activity were
enriched on the Con A column. WGA-separated populations were similar in sPro-N and sPro-O,
whereas Con A-separated ones were strikingly different.
Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context
of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic
and oligozoospermic men.
PB  - Upsala Medical Society
T2  - Upsala Journal of Medical Sciences
T1  - Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men
EP  - 18
IS  - 1
SP  - 10
VL  - 125
DO  - 10.1080/03009734.2019.1690603
ER  - 

@article{
author = "Janković, Tamara and Goč, Sanja and Mitic, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava",
year = "2019",
abstract = "Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs)
whose composition is, additionally, influenced by different physiological conditions. Aiming at the
molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction
factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference
could upgrade standardization of EVs isolated by different methods and intended for use in
biomedicine.
Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic
(sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
Prostasomes recovered in LAC-separated fractions were characterized according to the distribution
of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin
CD63, and total protein/glycoprotein composition.
Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing
in GGT activity and surface glycans were separated on the WGA column. Additional populations having
distinct profiles of total glycoproteins and which can be tracked down by ALP activity were
enriched on the Con A column. WGA-separated populations were similar in sPro-N and sPro-O,
whereas Con A-separated ones were strikingly different.
Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context
of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic
and oligozoospermic men.",
publisher = "Upsala Medical Society",
journal = "Upsala Journal of Medical Sciences",
title = "Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men",
pages = "18-10",
number = "1",
volume = "125",
doi = "10.1080/03009734.2019.1690603"
}

Janković, T., Goč, S., Mitic, N., Danilović Luković, J.,& Janković, M.. (2019). Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men. in Upsala Journal of Medical Sciences
Upsala Medical Society., 125(1), 10-18.
https://doi.org/10.1080/03009734.2019.1690603

Janković T, Goč S, Mitic N, Danilović Luković J, Janković M. Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men. in Upsala Journal of Medical Sciences. 2019;125(1):10-18.
doi:10.1080/03009734.2019.1690603 .

Janković, Tamara, Goč, Sanja, Mitic, Ninoslav, Danilović Luković, Jelena, Janković, Miroslava, "Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men" in Upsala Journal of Medical Sciences, 125, no. 1 (2019):10-18,
https://doi.org/10.1080/03009734.2019.1690603 . .