TY  - JOUR
AU  - Stajić, Mirjana
AU  - Kukavica, Biljana
AU  - Vukojevic, Jelena
AU  - Ćilerdžić, Jasmina
AU  - Veljovic Jovanovic, Sonja
AU  - Duletić-Laušević, Sonja
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3131
AB  - The purpose of this study was to resolve the question of whether various nitrogen sources and concentrations affect characteristics of selected G. lucidum ligninolytic enzymes participating in wheat straw fermentation. This is the first study reporting the presence of versatile peroxidase activity in crude extract of G. lucidum culture, as well as isoforms profile of Mn-oxidizing peroxidases. NH4NO3 was the optimum nitrogen source for laccase and Mn-dependent peroxidase activity, while peptone was the optimum one for versatile peroxidase activity. Four bands with laccase activity were obtained by native PAGE and IEF separations from medium enriched with inorganic nitrogen source, and only two bands from medium containing organic source. Medium composition was not shown to affect isoenzyme patterns of Mn-oxidizing peroxidases. Four isoforms of Mn-dependent peroxidase and three of versatile peroxidase were obtained on native PAGE. By IEF separation, five isoforms of Mn-dependent peroxidase and only two of versatile peroxidase were observed. The results demonstrated that G. lucidum has potential for mineralization and transformation of various agricultural residues and should take more significant participation in large-scale biotechnological processes.
PB  - NC State University
T2  - BioResources
T1  - WHEAT STRAW CONVERSION BY ENZYMATIC SYSTEM OF GANODERMA LUCIDUM
EP  - 2373
IS  - 5
SP  - 2362
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3131
ER  - 

@article{
author = "Stajić, Mirjana and Kukavica, Biljana and Vukojevic, Jelena and Ćilerdžić, Jasmina and Veljovic Jovanovic, Sonja and Duletić-Laušević, Sonja",
year = "2010",
abstract = "The purpose of this study was to resolve the question of whether various nitrogen sources and concentrations affect characteristics of selected G. lucidum ligninolytic enzymes participating in wheat straw fermentation. This is the first study reporting the presence of versatile peroxidase activity in crude extract of G. lucidum culture, as well as isoforms profile of Mn-oxidizing peroxidases. NH4NO3 was the optimum nitrogen source for laccase and Mn-dependent peroxidase activity, while peptone was the optimum one for versatile peroxidase activity. Four bands with laccase activity were obtained by native PAGE and IEF separations from medium enriched with inorganic nitrogen source, and only two bands from medium containing organic source. Medium composition was not shown to affect isoenzyme patterns of Mn-oxidizing peroxidases. Four isoforms of Mn-dependent peroxidase and three of versatile peroxidase were obtained on native PAGE. By IEF separation, five isoforms of Mn-dependent peroxidase and only two of versatile peroxidase were observed. The results demonstrated that G. lucidum has potential for mineralization and transformation of various agricultural residues and should take more significant participation in large-scale biotechnological processes.",
publisher = "NC State University",
journal = "BioResources",
title = "WHEAT STRAW CONVERSION BY ENZYMATIC SYSTEM OF GANODERMA LUCIDUM",
pages = "2373-2362",
number = "5",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3131"
}

Stajić, M., Kukavica, B., Vukojevic, J., Ćilerdžić, J., Veljovic Jovanovic, S.,& Duletić-Laušević, S.. (2010). WHEAT STRAW CONVERSION BY ENZYMATIC SYSTEM OF GANODERMA LUCIDUM. in BioResources
NC State University., 4(5), 2362-2373.
https://hdl.handle.net/21.15107/rcub_rimsi_3131

Stajić M, Kukavica B, Vukojevic J, Ćilerdžić J, Veljovic Jovanovic S, Duletić-Laušević S. WHEAT STRAW CONVERSION BY ENZYMATIC SYSTEM OF GANODERMA LUCIDUM. in BioResources. 2010;4(5):2362-2373.
https://hdl.handle.net/21.15107/rcub_rimsi_3131 .

Stajić, Mirjana, Kukavica, Biljana, Vukojevic, Jelena, Ćilerdžić, Jasmina, Veljovic Jovanovic, Sonja, Duletić-Laušević, Sonja, "WHEAT STRAW CONVERSION BY ENZYMATIC SYSTEM OF GANODERMA LUCIDUM" in BioResources, 4, no. 5 (2010):2362-2373,
https://hdl.handle.net/21.15107/rcub_rimsi_3131 .