TY  - CONF
AU  - Rodić, Ivanka
AU  - Lukičić, Jovana
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Živić, Miroslav
AU  - Cvetic Antic, Tijana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2409
AB  - Селенити могу бити веома токсични услед оксидативног стреса изазваног интеракцијама са унутарћелијским тиолима. Микробиолошка редукција селенита у елементарни селен, који је најмање токсичан облик селена, је од пресудног значаја за смањење биорасположивости овог елемента. Недавно је потврђено да гљива Phycomyces blakesleeanus има способност редукције селенита до елементарног селена. У овом раду је утврђено да селенит има инхибиторан ефекат на раст мицелије P. blakesleeanus при концентрацијама вишим од 500 μМ. Мицелије су усвајале око 30% селенита који се налазио у медијуму, а плато усвајања је достигнут на медијуму са 1 mM селенитом. Садржај глутатиона и активности ензима мерени су у мицелијама које су гајене у медијуму са 100 μM селенитом који није показивао токсичност. Већ након 3 ч третмана селенитом повећан је садржај оксидованог глутатиона код третираних мицелија у односу на контролу. Уочене су старосно зависне промене у активности ензима и концентрацији глутатиона које су се јављале код третираних мицелија и у контроли: смањење активности каталазе, смањење садржаја укупног глутатиона и повећање активности глутатион редуктазе 24 ч након преношења на свеж медијум са или без селенита. Активност каталазе, глутатион-редуктазе и -трансферазе се статистички значајно повећала у односу на контролу почев од 24 ч третмана селенитом, док је активност глутатион пероксидазе показала двофазни одговор, са повећањем активности 1 ч и 48 ч након преношења на медијум са селенитом
PB  - Srpsko biološko društvo, Beograd, 2022
C3  - Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
T1  - Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite
EP  - 233
SP  - 233
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2409
ER  - 

@conference{
author = "Rodić, Ivanka and Lukičić, Jovana and Stanić, Marina and Žižić, Milan and Zakrzewska, Joanna and Živić, Miroslav and Cvetic Antic, Tijana",
year = "2022",
abstract = "Селенити могу бити веома токсични услед оксидативног стреса изазваног интеракцијама са унутарћелијским тиолима. Микробиолошка редукција селенита у елементарни селен, који је најмање токсичан облик селена, је од пресудног значаја за смањење биорасположивости овог елемента. Недавно је потврђено да гљива Phycomyces blakesleeanus има способност редукције селенита до елементарног селена. У овом раду је утврђено да селенит има инхибиторан ефекат на раст мицелије P. blakesleeanus при концентрацијама вишим од 500 μМ. Мицелије су усвајале око 30% селенита који се налазио у медијуму, а плато усвајања је достигнут на медијуму са 1 mM селенитом. Садржај глутатиона и активности ензима мерени су у мицелијама које су гајене у медијуму са 100 μM селенитом који није показивао токсичност. Већ након 3 ч третмана селенитом повећан је садржај оксидованог глутатиона код третираних мицелија у односу на контролу. Уочене су старосно зависне промене у активности ензима и концентрацији глутатиона које су се јављале код третираних мицелија и у контроли: смањење активности каталазе, смањење садржаја укупног глутатиона и повећање активности глутатион редуктазе 24 ч након преношења на свеж медијум са или без селенита. Активност каталазе, глутатион-редуктазе и -трансферазе се статистички значајно повећала у односу на контролу почев од 24 ч третмана селенитом, док је активност глутатион пероксидазе показала двофазни одговор, са повећањем активности 1 ч и 48 ч након преношења на медијум са селенитом",
publisher = "Srpsko biološko društvo, Beograd, 2022",
journal = "Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.",
title = "Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite",
pages = "233-233",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2409"
}

Rodić, I., Lukičić, J., Stanić, M., Žižić, M., Zakrzewska, J., Živić, M.,& Cvetic Antic, T.. (2022). Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
Srpsko biološko društvo, Beograd, 2022., 233-233.
https://hdl.handle.net/21.15107/rcub_rimsi_2409

Rodić I, Lukičić J, Stanić M, Žižić M, Zakrzewska J, Živić M, Cvetic Antic T. Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.. 2022;:233-233.
https://hdl.handle.net/21.15107/rcub_rimsi_2409 .

Rodić, Ivanka, Lukičić, Jovana, Stanić, Marina, Žižić, Milan, Zakrzewska, Joanna, Živić, Miroslav, Cvetic Antic, Tijana, "Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite" in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022. (2022):233-233,
https://hdl.handle.net/21.15107/rcub_rimsi_2409 .

TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić Antič, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2385
AB  - Phycomyces blakesleeanus is a strict aerobic filamentous fungus often used as a model system in studies of physiology, genetics, environmental sensing, and metabolism. As all other aerobic organisms, this fungus faces the toxic effects of oxygen-reactive species, but data about its antioxidative defense systems are scarce. The aim of this research was to examine the activities of three antioxidant enzymes during different phases of growth. The fungus was grown in two ways, in Petri dishes, and on a shaker in Erlenmeyer flasks. The activities of superoxide dismutase
(SOD) and peroxidase (POD) were determined spectrophotometrically, while the activity of catalase (CAT) was determined polarographically with a Clark-type oxygen electrode. The highest activities of SOD were noticed in mycelia grown in Erlenmeyer flasks in the stationary phase of growth. In mycelia grown in Petri dishes, the highest activities of POD (0,014U/mg protein) and CAT (20,63 U/mg protein) were noticed in the early exponential phase. The activities of these two enzymes decreased with mycelial growth. In mycelia grown in Erlenmeyer flasks, POD and CAT
showed similar behavior, but differences in activities between exponential and stationary phases were smaller and the highest activities were noticed in the mid-exponential to stationary phase of growth (0,011U/mg protein for POD and 17,21U/mg protein for CAT). High activities of these two enzymes indicated increased production of H2O2 and pointed out the importance of this phase for mycelia grown in this way.
C3  - FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.
T1  - Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus
EP  - 858
SP  - 858
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2385
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetić Antič, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2022",
abstract = "Phycomyces blakesleeanus is a strict aerobic filamentous fungus often used as a model system in studies of physiology, genetics, environmental sensing, and metabolism. As all other aerobic organisms, this fungus faces the toxic effects of oxygen-reactive species, but data about its antioxidative defense systems are scarce. The aim of this research was to examine the activities of three antioxidant enzymes during different phases of growth. The fungus was grown in two ways, in Petri dishes, and on a shaker in Erlenmeyer flasks. The activities of superoxide dismutase
(SOD) and peroxidase (POD) were determined spectrophotometrically, while the activity of catalase (CAT) was determined polarographically with a Clark-type oxygen electrode. The highest activities of SOD were noticed in mycelia grown in Erlenmeyer flasks in the stationary phase of growth. In mycelia grown in Petri dishes, the highest activities of POD (0,014U/mg protein) and CAT (20,63 U/mg protein) were noticed in the early exponential phase. The activities of these two enzymes decreased with mycelial growth. In mycelia grown in Erlenmeyer flasks, POD and CAT
showed similar behavior, but differences in activities between exponential and stationary phases were smaller and the highest activities were noticed in the mid-exponential to stationary phase of growth (0,011U/mg protein for POD and 17,21U/mg protein for CAT). High activities of these two enzymes indicated increased production of H2O2 and pointed out the importance of this phase for mycelia grown in this way.",
journal = "FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.",
title = "Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus",
pages = "858-858",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2385"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetić Antič, T., Živić, M.,& Stanić, M.. (2022). Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia., 858-858.
https://hdl.handle.net/21.15107/rcub_rimsi_2385

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetić Antič T, Živić M, Stanić M. Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.. 2022;:858-858.
https://hdl.handle.net/21.15107/rcub_rimsi_2385 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetić Antič, Tijana, Živić, Miroslav, Stanić, Marina, "Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus" in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia. (2022):858-858,
https://hdl.handle.net/21.15107/rcub_rimsi_2385 .

TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetic Antic, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2418
AB  - Vanadium enters mycelium of fungus P. blakesleeanus in both +5 and +4 oxidation states. Mycelium in three stages of growth (20h, 36h and 56h) was treated with three concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. The decrease in viability of mycelium was noticed only at 36h for all applied concentrations. Glutathione has a role in reduction of V+5 to a less toxic form V+4 , so we assayed the changes in total glutathione. Mycelium in early exponential phase of growth (20 h), after 1h or 5h treatment, showed concentration dependent decrease in glutathione, with the largest decrease of 40±4% after 1h and 22±9% after 5h treatment induced by 10 mM V+5. In stationary phase of growth (56h), changes in glutathione concentration were noticed only after 1h of treatment, and the largest decrease (36±6%) was induced by 1 mM V+5. Activities of glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GPx), have also been examined. Increase in the activities of GPx and GST was noticed in 56h old mycelia treated for 5h with 5 mM and 10 mM V+5, but the only statistically significant increase (54±20%) was noticed in GST activity after 5h of 10 mM V+5 treatment.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia
T1  - Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus
EP  - 118
SP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2418
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetic Antic, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2019",
abstract = "Vanadium enters mycelium of fungus P. blakesleeanus in both +5 and +4 oxidation states. Mycelium in three stages of growth (20h, 36h and 56h) was treated with three concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. The decrease in viability of mycelium was noticed only at 36h for all applied concentrations. Glutathione has a role in reduction of V+5 to a less toxic form V+4 , so we assayed the changes in total glutathione. Mycelium in early exponential phase of growth (20 h), after 1h or 5h treatment, showed concentration dependent decrease in glutathione, with the largest decrease of 40±4% after 1h and 22±9% after 5h treatment induced by 10 mM V+5. In stationary phase of growth (56h), changes in glutathione concentration were noticed only after 1h of treatment, and the largest decrease (36±6%) was induced by 1 mM V+5. Activities of glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GPx), have also been examined. Increase in the activities of GPx and GST was noticed in 56h old mycelia treated for 5h with 5 mM and 10 mM V+5, but the only statistically significant increase (54±20%) was noticed in GST activity after 5h of 10 mM V+5 treatment.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia",
title = "Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus",
pages = "118-118",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2418"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetic Antic, T., Živić, M.,& Stanić, M.. (2019). Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus. in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 118-118.
https://hdl.handle.net/21.15107/rcub_rimsi_2418

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetic Antic T, Živić M, Stanić M. Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus. in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia. 2019;:118-118.
https://hdl.handle.net/21.15107/rcub_rimsi_2418 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetic Antic, Tijana, Živić, Miroslav, Stanić, Marina, "Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus" in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia (2019):118-118,
https://hdl.handle.net/21.15107/rcub_rimsi_2418 .