TY  - CONF
AU  - Vitkovac, Aleksandra
AU  - Pajić, Tanja
AU  - Bukumira, Marta
AU  - Stanić, Marina
AU  - Rabasovic, Mihailo
AU  - Todorović, Nataša
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2422
AB  - Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.
PB  - Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023
C3  - 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023
T1  - Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology
EP  - 337
SP  - 334
DO  - 10.46793/ICCBI23.334V
ER  - 

@conference{
author = "Vitkovac, Aleksandra and Pajić, Tanja and Bukumira, Marta and Stanić, Marina and Rabasovic, Mihailo and Todorović, Nataša",
year = "2023",
abstract = "Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.",
publisher = "Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023",
journal = "2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023",
title = "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology",
pages = "337-334",
doi = "10.46793/ICCBI23.334V"
}

Vitkovac, A., Pajić, T., Bukumira, M., Stanić, M., Rabasovic, M.,& Todorović, N.. (2023). Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023
Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023., 334-337.
https://doi.org/10.46793/ICCBI23.334V

Vitkovac A, Pajić T, Bukumira M, Stanić M, Rabasovic M, Todorović N. Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023. 2023;:334-337.
doi:10.46793/ICCBI23.334V .

Vitkovac, Aleksandra, Pajić, Tanja, Bukumira, Marta, Stanić, Marina, Rabasovic, Mihailo, Todorović, Nataša, "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology" in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023 (2023):334-337,
https://doi.org/10.46793/ICCBI23.334V . .

TY  - CONF
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - AQUILANTI, Giuliana
AU  - Cvetic Antic, Tijana
AU  - Todorović, Nataša
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2113
AB  - In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), with the aim of determining the flow and products of its biotransformation. Selenite is reduced to Se0 in the form selenium nanoparticles (SeNPs) and Se-2 in the form of methylated volatile selenides. Low concentrations of Se+4 in the mycelium form RSSeSR type compounds, which could be a step in the process of SeNPs formation, or incorporation of Se into metabolites such as Se-amino acids.
PB  - Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS
EP  - 96
SP  - 93
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2113
ER  - 

@conference{
author = "Žižić, Milan and Stanić, Marina and AQUILANTI, Giuliana and Cvetic Antic, Tijana and Todorović, Nataša and Živić, Miroslav and Zakrzewska, Joanna",
year = "2022",
abstract = "In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), with the aim of determining the flow and products of its biotransformation. Selenite is reduced to Se0 in the form selenium nanoparticles (SeNPs) and Se-2 in the form of methylated volatile selenides. Low concentrations of Se+4 in the mycelium form RSSeSR type compounds, which could be a step in the process of SeNPs formation, or incorporation of Se into metabolites such as Se-amino acids.",
publisher = "Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS",
pages = "96-93",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2113"
}

Žižić, M., Stanić, M., AQUILANTI, G., Cvetic Antic, T., Todorović, N., Živić, M.,& Zakrzewska, J.. (2022). SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia., 1, 93-96.
https://hdl.handle.net/21.15107/rcub_rimsi_2113

Žižić M, Stanić M, AQUILANTI G, Cvetic Antic T, Todorović N, Živić M, Zakrzewska J. SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;1:93-96.
https://hdl.handle.net/21.15107/rcub_rimsi_2113 .

Žižić, Milan, Stanić, Marina, AQUILANTI, Giuliana, Cvetic Antic, Tijana, Todorović, Nataša, Živić, Miroslav, Zakrzewska, Joanna, "SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 1 (2022):93-96,
https://hdl.handle.net/21.15107/rcub_rimsi_2113 .

TY  - CONF
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - Rodić, Ivanka
AU  - Cvetic Antic, Tijana
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
AU  - Todorović, Nataša
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2114
AB  - In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), to examine its ability to reduce it to nanoparticles. Red coloration appeared after only a few hours of incubation with 10 mM Se+4 indicating formation of selenium nanoparticles (SeNPs). SEM-EDS confirmed pure selenium NPs with an average diameter of 57 nm, which indicates to potentially very good medical, optical and photoelectric characteristics. Raman spectroscopy showed several structural forms of SeNPs formed in the extracellular space with a monoclinic Se8 chain as the most represented, and the other observed forms were trigonal Se polymer chain, Se8 ring and Se6 chain structures.
PB  - Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS
EP  - 92
SP  - 89
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2114
ER  - 

@conference{
author = "Žižić, Milan and Stanić, Marina and Rodić, Ivanka and Cvetic Antic, Tijana and Živić, Miroslav and Zakrzewska, Joanna and Todorović, Nataša",
year = "2022",
abstract = "In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), to examine its ability to reduce it to nanoparticles. Red coloration appeared after only a few hours of incubation with 10 mM Se+4 indicating formation of selenium nanoparticles (SeNPs). SEM-EDS confirmed pure selenium NPs with an average diameter of 57 nm, which indicates to potentially very good medical, optical and photoelectric characteristics. Raman spectroscopy showed several structural forms of SeNPs formed in the extracellular space with a monoclinic Se8 chain as the most represented, and the other observed forms were trigonal Se polymer chain, Se8 ring and Se6 chain structures.",
publisher = "Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS",
pages = "92-89",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2114"
}

Žižić, M., Stanić, M., Rodić, I., Cvetic Antic, T., Živić, M., Zakrzewska, J.,& Todorović, N.. (2022). PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia., 1, 89-92.
https://hdl.handle.net/21.15107/rcub_rimsi_2114

Žižić M, Stanić M, Rodić I, Cvetic Antic T, Živić M, Zakrzewska J, Todorović N. PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;1:89-92.
https://hdl.handle.net/21.15107/rcub_rimsi_2114 .

Žižić, Milan, Stanić, Marina, Rodić, Ivanka, Cvetic Antic, Tijana, Živić, Miroslav, Zakrzewska, Joanna, Todorović, Nataša, "PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 1 (2022):89-92,
https://hdl.handle.net/21.15107/rcub_rimsi_2114 .

TY  - JOUR
AU  - Avdović, Edina H.
AU  - Petrović, Isidora P.
AU  - Stevanović, Milena J.
AU  - Saso, Luciano
AU  - Dimitrić-Marković, Jasmina
AU  - Filipović, Nenad D.
AU  - Zivić, Miroslav
AU  - Cvetic, Antic, Tijana N.
AU  - Žižić, Milan
AU  - Todorović, Nataša
AU  - Vukic, Milena
AU  - Trifunović, Srecko R.
AU  - Marković, Zoran S.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1457
AB  - Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes
VL  - 2021
DO  - 10.1155/2021/8849568
ER  - 

@article{
author = "Avdović, Edina H. and Petrović, Isidora P. and Stevanović, Milena J. and Saso, Luciano and Dimitrić-Marković, Jasmina and Filipović, Nenad D. and Zivić, Miroslav and Cvetic, Antic, Tijana N. and Žižić, Milan and Todorović, Nataša and Vukic, Milena and Trifunović, Srecko R. and Marković, Zoran S.",
year = "2021",
abstract = "Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes",
volume = "2021",
doi = "10.1155/2021/8849568"
}

Avdović, E. H., Petrović, I. P., Stevanović, M. J., Saso, L., Dimitrić-Marković, J., Filipović, N. D., Zivić, M., Cvetic, A. T. N., Žižić, M., Todorović, N., Vukic, M., Trifunović, S. R.,& Marković, Z. S.. (2021). Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2021.
https://doi.org/10.1155/2021/8849568

Avdović EH, Petrović IP, Stevanović MJ, Saso L, Dimitrić-Marković J, Filipović ND, Zivić M, Cvetic ATN, Žižić M, Todorović N, Vukic M, Trifunović SR, Marković ZS. Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity. 2021;2021.
doi:10.1155/2021/8849568 .

Avdović, Edina H., Petrović, Isidora P., Stevanović, Milena J., Saso, Luciano, Dimitrić-Marković, Jasmina, Filipović, Nenad D., Zivić, Miroslav, Cvetic, Antic, Tijana N., Žižić, Milan, Todorović, Nataša, Vukic, Milena, Trifunović, Srecko R., Marković, Zoran S., "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes" in Oxidative Medicine and Cellular Longevity, 2021 (2021),
https://doi.org/10.1155/2021/8849568 . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Jovanović, Mirna
AU  - Pajic, Tanja
AU  - Ciric, Ana
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Todorović, Nataša
AU  - Zivić, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1082
AB  - Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 mu M NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33 +/- 5 and 21 +/- 3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.
PB  - Microbiology Soc, London
T2  - Microbiology-Sgm
T1  - Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites
EP  - 372
IS  - 3
SP  - 364
VL  - 163
DO  - 10.1099/mic.0.000429
ER  - 

@article{
author = "Stanić, Marina and Krizak, Strahinja and Jovanović, Mirna and Pajic, Tanja and Ciric, Ana and Žižić, Milan and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Todorović, Nataša and Zivić, Miroslav",
year = "2017",
abstract = "Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 mu M NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33 +/- 5 and 21 +/- 3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.",
publisher = "Microbiology Soc, London",
journal = "Microbiology-Sgm",
title = "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites",
pages = "372-364",
number = "3",
volume = "163",
doi = "10.1099/mic.0.000429"
}

Stanić, M., Krizak, S., Jovanović, M., Pajic, T., Ciric, A., Žižić, M., Zakrzewska, J., Cvetić-Antić, T., Todorović, N.,& Zivić, M.. (2017). Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology-Sgm
Microbiology Soc, London., 163(3), 364-372.
https://doi.org/10.1099/mic.0.000429

Stanić M, Krizak S, Jovanović M, Pajic T, Ciric A, Žižić M, Zakrzewska J, Cvetić-Antić T, Todorović N, Zivić M. Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology-Sgm. 2017;163(3):364-372.
doi:10.1099/mic.0.000429 .

Stanić, Marina, Krizak, Strahinja, Jovanović, Mirna, Pajic, Tanja, Ciric, Ana, Žižić, Milan, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Todorović, Nataša, Zivić, Miroslav, "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites" in Microbiology-Sgm, 163, no. 3 (2017):364-372,
https://doi.org/10.1099/mic.0.000429 . .

TY  - JOUR
AU  - Krizak, Strahinja
AU  - Nikolić, Ljiljana
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Zivić, Miroslav
AU  - Todorović, Nataša
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/869
AB  - We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores
EP  - 173
IS  - 3
SP  - 162
VL  - 166
DO  - 10.1016/j.resmic.2015.02.004
ER  - 

@article{
author = "Krizak, Strahinja and Nikolić, Ljiljana and Stanić, Marina and Žižić, Milan and Zakrzewska, Joanna and Zivić, Miroslav and Todorović, Nataša",
year = "2015",
abstract = "We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores",
pages = "173-162",
number = "3",
volume = "166",
doi = "10.1016/j.resmic.2015.02.004"
}

Krizak, S., Nikolić, L., Stanić, M., Žižić, M., Zakrzewska, J., Zivić, M.,& Todorović, N.. (2015). Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores. in Research in Microbiology
Elsevier, Amsterdam., 166(3), 162-173.
https://doi.org/10.1016/j.resmic.2015.02.004

Krizak S, Nikolić L, Stanić M, Žižić M, Zakrzewska J, Zivić M, Todorović N. Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores. in Research in Microbiology. 2015;166(3):162-173.
doi:10.1016/j.resmic.2015.02.004 .

Krizak, Strahinja, Nikolić, Ljiljana, Stanić, Marina, Žižić, Milan, Zakrzewska, Joanna, Zivić, Miroslav, Todorović, Nataša, "Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores" in Research in Microbiology, 166, no. 3 (2015):162-173,
https://doi.org/10.1016/j.resmic.2015.02.004 . .

TY  - JOUR
AU  - Nikolić, Ljiljana M.
AU  - Todorović, Nataša
AU  - Zakrzewska, Joanna
AU  - Stanić, Marina
AU  - Raus, Snežana Z
AU  - Kalauzi, Aleksandar
AU  - Janac, Branka B
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/539
AB  - The spontaneously active Br neuron from the brain-subesophageal ganglion complex of the garden snail Helix pomatia rhythmically generates regular bursts of action potentials with quiescent intervals accompanied by slow oscillations of membrane potential. We examined the involvement of the Na+/K+ pump in modulating its bursting activity by applying a static magnetic field. Whole snail brains and Br neuron were exposed to the 10-mT static magnetic field for 15 min. Biochemical data showed that Na+/K+-ATPase activity increased almost twofold after exposure of snail brains to the static magnetic field. Similarly, P-31 NMR data revealed a trend of increasing ATP consumption and increase in intracellular pH mediated by the Na+/H+ exchanger in snail brains exposed to the static magnetic field. Importantly, current clamp recordings from the Br neuron confirmed the increase in activity of the Na+/K+ pump after exposure to the static magnetic field, as the magnitude of ouabain's effect measured on the membrane resting potential, action potential, and interspike interval duration was higher in neurons exposed to the magnetic field. Metabolic pathways through which the magnetic field influenced the Na+/K+ pump could involve phosphorylation and dephosphorylation, as blocking these processes abolished the effect of the static magnetic field.
PB  - Springer, New York
T2  - Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology
T1  - Involvement of Na+/K(+)pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field
EP  - 540
IS  - 7
SP  - 525
VL  - 198
DO  - 10.1007/s00359-012-0727-0
ER  - 

@article{
author = "Nikolić, Ljiljana M. and Todorović, Nataša and Zakrzewska, Joanna and Stanić, Marina and Raus, Snežana Z and Kalauzi, Aleksandar and Janac, Branka B",
year = "2012",
abstract = "The spontaneously active Br neuron from the brain-subesophageal ganglion complex of the garden snail Helix pomatia rhythmically generates regular bursts of action potentials with quiescent intervals accompanied by slow oscillations of membrane potential. We examined the involvement of the Na+/K+ pump in modulating its bursting activity by applying a static magnetic field. Whole snail brains and Br neuron were exposed to the 10-mT static magnetic field for 15 min. Biochemical data showed that Na+/K+-ATPase activity increased almost twofold after exposure of snail brains to the static magnetic field. Similarly, P-31 NMR data revealed a trend of increasing ATP consumption and increase in intracellular pH mediated by the Na+/H+ exchanger in snail brains exposed to the static magnetic field. Importantly, current clamp recordings from the Br neuron confirmed the increase in activity of the Na+/K+ pump after exposure to the static magnetic field, as the magnitude of ouabain's effect measured on the membrane resting potential, action potential, and interspike interval duration was higher in neurons exposed to the magnetic field. Metabolic pathways through which the magnetic field influenced the Na+/K+ pump could involve phosphorylation and dephosphorylation, as blocking these processes abolished the effect of the static magnetic field.",
publisher = "Springer, New York",
journal = "Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology",
title = "Involvement of Na+/K(+)pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field",
pages = "540-525",
number = "7",
volume = "198",
doi = "10.1007/s00359-012-0727-0"
}

Nikolić, L. M., Todorović, N., Zakrzewska, J., Stanić, M., Raus, S. Z., Kalauzi, A.,& Janac, B. B.. (2012). Involvement of Na+/K(+)pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field. in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology
Springer, New York., 198(7), 525-540.
https://doi.org/10.1007/s00359-012-0727-0

Nikolić LM, Todorović N, Zakrzewska J, Stanić M, Raus SZ, Kalauzi A, Janac BB. Involvement of Na+/K(+)pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field. in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology. 2012;198(7):525-540.
doi:10.1007/s00359-012-0727-0 .

Nikolić, Ljiljana M., Todorović, Nataša, Zakrzewska, Joanna, Stanić, Marina, Raus, Snežana Z, Kalauzi, Aleksandar, Janac, Branka B, "Involvement of Na+/K(+)pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field" in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology, 198, no. 7 (2012):525-540,
https://doi.org/10.1007/s00359-012-0727-0 . .