TY  - JOUR
AU  - Pejin, Boris
AU  - Stosic-Grujicic, Stanislava
AU  - Bogdanović, Gordana
AU  - Hegediš, Aleksandar
AU  - Karaman, I.
AU  - Stojanović, I.
AU  - Nikolić, Ivan
AU  - Kojic, V.
AU  - Horvatović, Mladen
AU  - Radotić, Ksenija
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/619
AB  - The immunomodulatory and anticarcinogenic activity of the freshwater bryozoan Hyalinella punctata methanolic extract (MEx) was evaluated in vitro on selected biosystems for the first time. Murine lymphocytes and macrophages were used for testing of MEx effects on cell proliferation and nitric oxide (NO) production, respectively, while human cancer cell lines were utilized for measuring its activity against cancer cells. The results suggest a strong and tissue-specific immunomodifying activity (IC50 values for inhibition of proliferation of lymph node and spleen-derived lymphocytes were 4.1 mu g/ml and 9.7 mu g/ml, respectively) and moderate anticancer activity of MEx (IC50 value for the MCF-7 cell line was 24.13 mu g/ml). Down-regulation of macrophage NO production was also obtained. The potential use of H. punctata-derived natural products for the treatment of human chronic inflammatory diseases and cancer is worthy of further investigation.
PB  - Inst Materials Physics
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - In vitro evaluation of the immunomodulatory and anticarcinogenic activity of the freshwater bryozoan hyalinella punctata methanolic extract
EP  - 195
IS  - 1
SP  - 187
VL  - 8
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_619
ER  - 

@article{
author = "Pejin, Boris and Stosic-Grujicic, Stanislava and Bogdanović, Gordana and Hegediš, Aleksandar and Karaman, I. and Stojanović, I. and Nikolić, Ivan and Kojic, V. and Horvatović, Mladen and Radotić, Ksenija",
year = "2012",
abstract = "The immunomodulatory and anticarcinogenic activity of the freshwater bryozoan Hyalinella punctata methanolic extract (MEx) was evaluated in vitro on selected biosystems for the first time. Murine lymphocytes and macrophages were used for testing of MEx effects on cell proliferation and nitric oxide (NO) production, respectively, while human cancer cell lines were utilized for measuring its activity against cancer cells. The results suggest a strong and tissue-specific immunomodifying activity (IC50 values for inhibition of proliferation of lymph node and spleen-derived lymphocytes were 4.1 mu g/ml and 9.7 mu g/ml, respectively) and moderate anticancer activity of MEx (IC50 value for the MCF-7 cell line was 24.13 mu g/ml). Down-regulation of macrophage NO production was also obtained. The potential use of H. punctata-derived natural products for the treatment of human chronic inflammatory diseases and cancer is worthy of further investigation.",
publisher = "Inst Materials Physics",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "In vitro evaluation of the immunomodulatory and anticarcinogenic activity of the freshwater bryozoan hyalinella punctata methanolic extract",
pages = "195-187",
number = "1",
volume = "8",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_619"
}

Pejin, B., Stosic-Grujicic, S., Bogdanović, G., Hegediš, A., Karaman, I., Stojanović, I., Nikolić, I., Kojic, V., Horvatović, M.,& Radotić, K.. (2012). In vitro evaluation of the immunomodulatory and anticarcinogenic activity of the freshwater bryozoan hyalinella punctata methanolic extract. in Digest Journal of Nanomaterials and Biostructures
Inst Materials Physics., 8(1), 187-195.
https://hdl.handle.net/21.15107/rcub_rimsi_619

Pejin B, Stosic-Grujicic S, Bogdanović G, Hegediš A, Karaman I, Stojanović I, Nikolić I, Kojic V, Horvatović M, Radotić K. In vitro evaluation of the immunomodulatory and anticarcinogenic activity of the freshwater bryozoan hyalinella punctata methanolic extract. in Digest Journal of Nanomaterials and Biostructures. 2012;8(1):187-195.
https://hdl.handle.net/21.15107/rcub_rimsi_619 .

Pejin, Boris, Stosic-Grujicic, Stanislava, Bogdanović, Gordana, Hegediš, Aleksandar, Karaman, I., Stojanović, I., Nikolić, Ivan, Kojic, V., Horvatović, Mladen, Radotić, Ksenija, "In vitro evaluation of the immunomodulatory and anticarcinogenic activity of the freshwater bryozoan hyalinella punctata methanolic extract" in Digest Journal of Nanomaterials and Biostructures, 8, no. 1 (2012):187-195,
https://hdl.handle.net/21.15107/rcub_rimsi_619 .

TY  - JOUR
AU  - Harhaji, Lj.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, I.
AU  - Momcilović, Miljana
AU  - Maksimović, Vuk
AU  - Tufegdžić, Srđan J.
AU  - Marjanović, Žaklina
AU  - Mostarica-Stojković, M.
AU  - Vučinić, Željko
AU  - Stosic-Grujicic, Stanislava
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/262
AB  - Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study
EP  - 1833
IS  - 5
SP  - 1825
VL  - 46
DO  - 10.1016/j.fct.2008.01.027
ER  - 

@article{
author = "Harhaji, Lj. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, I. and Momcilović, Miljana and Maksimović, Vuk and Tufegdžić, Srđan J. and Marjanović, Žaklina and Mostarica-Stojković, M. and Vučinić, Željko and Stosic-Grujicic, Stanislava",
year = "2008",
abstract = "Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study",
pages = "1833-1825",
number = "5",
volume = "46",
doi = "10.1016/j.fct.2008.01.027"
}

Harhaji, Lj., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I., Momcilović, M., Maksimović, V., Tufegdžić, S. J., Marjanović, Ž., Mostarica-Stojković, M., Vučinić, Ž.,& Stosic-Grujicic, S.. (2008). Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 46(5), 1825-1833.
https://doi.org/10.1016/j.fct.2008.01.027

Harhaji L, Mijatović S, Maksimović-Ivanić D, Stojanović I, Momcilović M, Maksimović V, Tufegdžić SJ, Marjanović Ž, Mostarica-Stojković M, Vučinić Ž, Stosic-Grujicic S. Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology. 2008;46(5):1825-1833.
doi:10.1016/j.fct.2008.01.027 .

Harhaji, Lj., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, I., Momcilović, Miljana, Maksimović, Vuk, Tufegdžić, Srđan J., Marjanović, Žaklina, Mostarica-Stojković, M., Vučinić, Željko, Stosic-Grujicic, Stanislava, "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study" in Food and Chemical Toxicology, 46, no. 5 (2008):1825-1833,
https://doi.org/10.1016/j.fct.2008.01.027 . .