TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Milošković, Aleksandra
AU  - Radojković, Nataša
AU  - Simić, Vera
AU  - Dojčinović, Biljana
AU  - Kračun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Timilijić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2150
AB  - Abstract:
Introduction. The SOS/umuC assay is used for assessment of water genotoxicity. Salmonella typhimurium TA1535/pSK1002 is used in this test, but the results can be extrapolated on higher eukaryotic organisms with the introduction of enzymatic S9 fraction in experimental procedure. The test is standardised for the determination of the genotoxic potential of water and wastewater (ISO/DIS 13829, 2000). Aim. Sensitivity of the assay was challenged by using parallel in situ and in vitro approach in evaluation of the genotoxic potential in the basins of significant tributaries of the Danube River: the Sava River and the Velika Morava River. Materials and methods. Within in vitro testing, native water samples were analysed by SOS/umuC test. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet and micronucleus assays. The concentration of heavy metals in fish tissue and the data of the physico-chemical parameters measured in water were used as a measure of the pollution pressure at the sites. Results. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests. None of 20 investigated samples showed genotoxic potential in SOS/umuC assay while in situ analyses indicated variation of genotoxic potential among the investigated sites. Conclusions. The results of our study point towards low sensitivity of the SOS/umuC test when processing un-concentrated (native) water samples; the results of in vitro tests should be taken with precaution when making predictions on the status of the ecosystem.
PB  - Balkan Society for Microbiology
C3  - 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017
T1  - Application of sos/umuc assay in eco/genotoxicology
EP  - 385
SP  - 385
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2150
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Milošković, Aleksandra and Radojković, Nataša and Simić, Vera and Dojčinović, Biljana and Kračun-Kolarević, Margareta and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Timilijić, Jovana and Đorđević Aleksić, Jelena and Gačić, Zoran and Vuković-Gačić, Branka",
year = "2017",
abstract = "Abstract:
Introduction. The SOS/umuC assay is used for assessment of water genotoxicity. Salmonella typhimurium TA1535/pSK1002 is used in this test, but the results can be extrapolated on higher eukaryotic organisms with the introduction of enzymatic S9 fraction in experimental procedure. The test is standardised for the determination of the genotoxic potential of water and wastewater (ISO/DIS 13829, 2000). Aim. Sensitivity of the assay was challenged by using parallel in situ and in vitro approach in evaluation of the genotoxic potential in the basins of significant tributaries of the Danube River: the Sava River and the Velika Morava River. Materials and methods. Within in vitro testing, native water samples were analysed by SOS/umuC test. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet and micronucleus assays. The concentration of heavy metals in fish tissue and the data of the physico-chemical parameters measured in water were used as a measure of the pollution pressure at the sites. Results. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests. None of 20 investigated samples showed genotoxic potential in SOS/umuC assay while in situ analyses indicated variation of genotoxic potential among the investigated sites. Conclusions. The results of our study point towards low sensitivity of the SOS/umuC test when processing un-concentrated (native) water samples; the results of in vitro tests should be taken with precaution when making predictions on the status of the ecosystem.",
publisher = "Balkan Society for Microbiology",
journal = "10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017",
title = "Application of sos/umuc assay in eco/genotoxicology",
pages = "385-385",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2150"
}

Jovanović Marić, J., Kolarević, S., Milošković, A., Radojković, N., Simić, V., Dojčinović, B., Kračun-Kolarević, M., Paunović, M., Kostić-Vuković, J., Sunjog, K., Timilijić, J., Đorđević Aleksić, J., Gačić, Z.,& Vuković-Gačić, B.. (2017). Application of sos/umuc assay in eco/genotoxicology. in 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017
Balkan Society for Microbiology., 385-385.
https://hdl.handle.net/21.15107/rcub_rimsi_2150

Jovanović Marić J, Kolarević S, Milošković A, Radojković N, Simić V, Dojčinović B, Kračun-Kolarević M, Paunović M, Kostić-Vuković J, Sunjog K, Timilijić J, Đorđević Aleksić J, Gačić Z, Vuković-Gačić B. Application of sos/umuc assay in eco/genotoxicology. in 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017. 2017;:385-385.
https://hdl.handle.net/21.15107/rcub_rimsi_2150 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Milošković, Aleksandra, Radojković, Nataša, Simić, Vera, Dojčinović, Biljana, Kračun-Kolarević, Margareta, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Timilijić, Jovana, Đorđević Aleksić, Jelena, Gačić, Zoran, Vuković-Gačić, Branka, "Application of sos/umuc assay in eco/genotoxicology" in 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017 (2017):385-385,
https://hdl.handle.net/21.15107/rcub_rimsi_2150 .