@conference{
author = "Popović, Tatjana and Jelušić, Aleksandra and Aćimović, Radivoje and Marković, Sanja and Iličić, Renata",
year = "2022",
abstract = "The vegetable soybean “edamame” (Glicine max L.) is a
nutritious legume having pods and seeds that can be
harvested and consumed while they are still fresh and
premature. Edamame is rich in micronutrients and
vitamins and is therefore used as food and for medicinal
purposes. In Serbia, edamame production started for the
first time in 2019 on a 10 ha field in Bečej locality (Bačka,
Vojvodina). Later, in 2021, its production was expanded
to 30 ha, but in summer was followed with symptoms of
bacterial leaf spot. The symptoms appeared on leaves in
the form of water-soaked spots surrounded by a chlorotic
halo that enlarged and coalesced into necrotic lesions.
Disease incidence was from 15-20%. Isolation of the
causal pathogen was performed by sowing of suspension
of the macerated margins of spots/lesions from ten
collected symptomatic leaves on nutrient agar
supplemented with 5% sucrose. Ten representative,
purified isolates were whitish, circular, smooth, shiny,
levan-positive, strictly aerobic, gram-negative; positive for
green-fluorescent pigment and tobacco hypersensitive
response, and negative for oxidase, arginine dihydrolase,
and potato soft rot (LOPAT group Ia). Pathogenicity of the
isolates was confirmed on soybean cotyledons by under
pressure atomizing a bacterial suspension (107-8 CFU mL 1
). Sequencing of genes gapA, gyrB, and rpoD showed
100% homology of the obtained isolates with
Pseudomonas savastanoi pv. glycinea pathotype strain
LMG 5066 and strains BR1, KN166, KN28, KN44, LN10,
MOC601, R4a (gapA and gyrB), and M301765 (gapA and
rpoD) originated from soybean, all from the Plant
Associated and Environmental Microbes Database
(PAMDB).",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference on Plant Pathogenic Bacteria",
title = "Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1958"
}