TY  - JOUR
AU  - Bosnic, Dragana
AU  - Bosnić, Predrag
AU  - Nikolic, Dragana
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1220
AB  - Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves
IS  - 12
VL  - 8
DO  - 10.3390/plants8120554
ER  - 

@article{
author = "Bosnic, Dragana and Bosnić, Predrag and Nikolic, Dragana and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2019",
abstract = "Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves",
number = "12",
volume = "8",
doi = "10.3390/plants8120554"
}

Bosnic, D., Bosnić, P., Nikolic, D., Nikolic, M.,& Samardžić, J. T.. (2019). Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel
MDPI, Basel., 8(12).
https://doi.org/10.3390/plants8120554

Bosnic D, Bosnić P, Nikolic D, Nikolic M, Samardžić JT. Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel. 2019;8(12).
doi:10.3390/plants8120554 .

Bosnic, Dragana, Bosnić, Predrag, Nikolic, Dragana, Nikolic, Miroslav, Samardžić, Jelena T., "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves" in Plants-Basel, 8, no. 12 (2019),
https://doi.org/10.3390/plants8120554 . .

TY  - JOUR
AU  - Nikolic, Dragana B.
AU  - Nesic, Sofija
AU  - Bosnic, Dragana
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1284
AB  - The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
VL  - 10
DO  - 10.3389/fpls.2019.00416
ER  - 

@article{
author = "Nikolic, Dragana B. and Nesic, Sofija and Bosnic, Dragana and Kostić Kravljanac, Ljiljana and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2019",
abstract = "The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution",
volume = "10",
doi = "10.3389/fpls.2019.00416"
}

Nikolic, D. B., Nesic, S., Bosnic, D., Kostić Kravljanac, L., Nikolic, M.,& Samardžić, J. T.. (2019). Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fpls.2019.00416

Nikolic DB, Nesic S, Bosnic D, Kostić Kravljanac L, Nikolic M, Samardžić JT. Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science. 2019;10.
doi:10.3389/fpls.2019.00416 .

Nikolic, Dragana B., Nesic, Sofija, Bosnic, Dragana, Kostić Kravljanac, Ljiljana, Nikolic, Miroslav, Samardžić, Jelena T., "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution" in Frontiers in Plant Science, 10 (2019),
https://doi.org/10.3389/fpls.2019.00416 . .

TY  - JOUR
AU  - Bosnic, Dragana
AU  - Nikolic, Dragana
AU  - Timotijević, Gordana
AU  - Pavlović, Jelena
AU  - Vaculik, Marek
AU  - Samardžić, Jelena T.
AU  - Nikolic, Miroslav
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1212
AB  - Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity
EP  - 641
IS  - 1-2
SP  - 629
VL  - 441
DO  - 10.1007/s11104-019-04151-5
ER  - 

@article{
author = "Bosnic, Dragana and Nikolic, Dragana and Timotijević, Gordana and Pavlović, Jelena and Vaculik, Marek and Samardžić, Jelena T. and Nikolic, Miroslav",
year = "2019",
abstract = "Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity",
pages = "641-629",
number = "1-2",
volume = "441",
doi = "10.1007/s11104-019-04151-5"
}

Bosnic, D., Nikolic, D., Timotijević, G., Pavlović, J., Vaculik, M., Samardžić, J. T.,& Nikolic, M.. (2019). Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil
Springer, Dordrecht., 441(1-2), 629-641.
https://doi.org/10.1007/s11104-019-04151-5

Bosnic D, Nikolic D, Timotijević G, Pavlović J, Vaculik M, Samardžić JT, Nikolic M. Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil. 2019;441(1-2):629-641.
doi:10.1007/s11104-019-04151-5 .

Bosnic, Dragana, Nikolic, Dragana, Timotijević, Gordana, Pavlović, Jelena, Vaculik, Marek, Samardžić, Jelena T., Nikolic, Miroslav, "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity" in Plant and Soil, 441, no. 1-2 (2019):629-641,
https://doi.org/10.1007/s11104-019-04151-5 . .

TY  - JOUR
AU  - Savić, J.
AU  - Stević, N.
AU  - Maksimović, Vuk
AU  - Samardžić, Jelena T.
AU  - Nikolic, D.B.
AU  - Nikolic, M.
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1179
AB  - Aluminium (Al) toxicity in acid soils is a global problem. Here, we investigated Al tolerance in high yielding winter wheat (Triticum aestivum L.) cultivars bred in Serbia. The common relative mot length (RRL) test for Al tolerance, and both physiological (malate efflux) and molecular (Aluminium-Activated Malate Transporter 1 [TaALMT1] expression) approaches were used for this characterization. Both moderately Al-tolerant cvs. Ljiljana and Arabeska showed significantly higher malate efflux rate from the root tips in comparison to moderately Al-sensitive cv. Pobeda and followed the RRL pattern. Irrespectively of Al supply, moderately Al-tolerant cultivars showed significantly higher relative TaALMT1 expression than the Al-sensitive ones. A considerably high level of Al tolerance was found in cv. Ljiljana, which showed the highest Al-induced malate efflux along with the highest constitutive expression level of TaALMT1 transcripts. Our results also demonstrate that Al tolerance is based on a constitutive trait of high TaALMT1 expression and malate efflux in wheat roots, resulting in a decrease in root length reduction.
PB  - Sociedad Chilena de la Ciencia del Suelo
T2  - Journal of Soil Science and Plant Nutrition
T1  - Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance
EP  - 99
IS  - 1
SP  - 90
VL  - 18
DO  - 10.4067/S0718-95162018005000402
ER  - 

@article{
author = "Savić, J. and Stević, N. and Maksimović, Vuk and Samardžić, Jelena T. and Nikolic, D.B. and Nikolic, M.",
year = "2018",
abstract = "Aluminium (Al) toxicity in acid soils is a global problem. Here, we investigated Al tolerance in high yielding winter wheat (Triticum aestivum L.) cultivars bred in Serbia. The common relative mot length (RRL) test for Al tolerance, and both physiological (malate efflux) and molecular (Aluminium-Activated Malate Transporter 1 [TaALMT1] expression) approaches were used for this characterization. Both moderately Al-tolerant cvs. Ljiljana and Arabeska showed significantly higher malate efflux rate from the root tips in comparison to moderately Al-sensitive cv. Pobeda and followed the RRL pattern. Irrespectively of Al supply, moderately Al-tolerant cultivars showed significantly higher relative TaALMT1 expression than the Al-sensitive ones. A considerably high level of Al tolerance was found in cv. Ljiljana, which showed the highest Al-induced malate efflux along with the highest constitutive expression level of TaALMT1 transcripts. Our results also demonstrate that Al tolerance is based on a constitutive trait of high TaALMT1 expression and malate efflux in wheat roots, resulting in a decrease in root length reduction.",
publisher = "Sociedad Chilena de la Ciencia del Suelo",
journal = "Journal of Soil Science and Plant Nutrition",
title = "Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance",
pages = "99-90",
number = "1",
volume = "18",
doi = "10.4067/S0718-95162018005000402"
}

Savić, J., Stević, N., Maksimović, V., Samardžić, J. T., Nikolic, D.B.,& Nikolic, M.. (2018). Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance. in Journal of Soil Science and Plant Nutrition
Sociedad Chilena de la Ciencia del Suelo., 18(1), 90-99.
https://doi.org/10.4067/S0718-95162018005000402

Savić J, Stević N, Maksimović V, Samardžić JT, Nikolic D, Nikolic M. Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance. in Journal of Soil Science and Plant Nutrition. 2018;18(1):90-99.
doi:10.4067/S0718-95162018005000402 .

Savić, J., Stević, N., Maksimović, Vuk, Samardžić, Jelena T., Nikolic, D.B., Nikolic, M., "Root malate efflux and expression of TaALMT1 in Serbian winter wheat cultivars differing in Al tolerance" in Journal of Soil Science and Plant Nutrition, 18, no. 1 (2018):90-99,
https://doi.org/10.4067/S0718-95162018005000402 . .

TY  - JOUR
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolić, Nina
AU  - Bosnic, Dragana
AU  - Samardžić, Jelena T.
AU  - Nikolic, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1067
AB  - Although silicon (Si) is known to improve plant growth under low phosphorus (P) conditions, the in planta mechanisms responsible for this effect are still unknown. Here, we investigated the role of Si on P uptake along with the expression of Pi transporters in wheat (Triticum aestivum L.) grown in low P acid soil in comparison with P fertilization and liming. A combined approach was performed including analyses of rhizosphere soil, tissue P content, the expression of the root Pi transporter genes (TaPHT1.1 and TaPHT1.2), and the root exudation of citrate and malate. Supply of Si in a form of Na2SiO3 increased shoot P concentration to an adequate level in the range of P-fertilized plants. Silicon ameliorated low soil pH and high Al3+ comparable to the effect of liming. The in planta effect of Si on up-regulating the expression of TaPHT1.1 and TaPHT1.2 was several fold higher and consequently P uptake doubled compared to both P fertilization and liming. In addition, Si directly stimulated root Pi acquisition by prominently increasing both malate and citrate exudation rate. Application of Si increased root exudation of organic acids that mobilize Pi in the rhizosphere and up-regulated Pi transporters in wheat roots.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions
EP  - 455
IS  - 1-2
SP  - 447
VL  - 419
DO  - 10.1007/s11104-017-3364-0
ER  - 

@article{
author = "Kostić Kravljanac, Ljiljana and Nikolić, Nina and Bosnic, Dragana and Samardžić, Jelena T. and Nikolic, Miroslav",
year = "2017",
abstract = "Although silicon (Si) is known to improve plant growth under low phosphorus (P) conditions, the in planta mechanisms responsible for this effect are still unknown. Here, we investigated the role of Si on P uptake along with the expression of Pi transporters in wheat (Triticum aestivum L.) grown in low P acid soil in comparison with P fertilization and liming. A combined approach was performed including analyses of rhizosphere soil, tissue P content, the expression of the root Pi transporter genes (TaPHT1.1 and TaPHT1.2), and the root exudation of citrate and malate. Supply of Si in a form of Na2SiO3 increased shoot P concentration to an adequate level in the range of P-fertilized plants. Silicon ameliorated low soil pH and high Al3+ comparable to the effect of liming. The in planta effect of Si on up-regulating the expression of TaPHT1.1 and TaPHT1.2 was several fold higher and consequently P uptake doubled compared to both P fertilization and liming. In addition, Si directly stimulated root Pi acquisition by prominently increasing both malate and citrate exudation rate. Application of Si increased root exudation of organic acids that mobilize Pi in the rhizosphere and up-regulated Pi transporters in wheat roots.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions",
pages = "455-447",
number = "1-2",
volume = "419",
doi = "10.1007/s11104-017-3364-0"
}

Kostić Kravljanac, L., Nikolić, N., Bosnic, D., Samardžić, J. T.,& Nikolic, M.. (2017). Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions. in Plant and Soil
Springer, Dordrecht., 419(1-2), 447-455.
https://doi.org/10.1007/s11104-017-3364-0

Kostić Kravljanac L, Nikolić N, Bosnic D, Samardžić JT, Nikolic M. Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions. in Plant and Soil. 2017;419(1-2):447-455.
doi:10.1007/s11104-017-3364-0 .

Kostić Kravljanac, Ljiljana, Nikolić, Nina, Bosnic, Dragana, Samardžić, Jelena T., Nikolic, Miroslav, "Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions" in Plant and Soil, 419, no. 1-2 (2017):447-455,
https://doi.org/10.1007/s11104-017-3364-0 . .

TY  - JOUR
AU  - Pavlović, Jelena
AU  - Samardžić, Jelena T.
AU  - Kostić Kravljanac, Ljiljana
AU  - Laursen, Kristian H.
AU  - Natic, Maja
AU  - Timotijević, Gordana
AU  - Schjoerring, Jan K.
AU  - Nikolic, Miroslav
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1002
AB  - Background and Aims Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe deficiency. Previous work has shown that silicon (Si) can alleviate Fe deficiency by enhancing acquisition and root to shoot translocation of Fe. Here the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe-limiting conditions was investigated. Methods Iron (Fe-57 or naturally occurring isotopes) was measured in leaves at different positions on plants hydroponically growing with or without Si supply. In parallel, the concentration of the Fe chelator nicotianamine (NA) along with the expression of nicotianamine synthase (NAS) involved in its biosynthesis and the expression of yellow stripe-like (YSL) transcripts mediating Fe-NA transport were also determined. Key Results In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. In contrast, Si-treated plants showed an almost even Fe distribution among leaves with four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This Si-stimulated Fe export was paralleled by an increased NA accumulation and expression of the YSL1 transporter for phloem loading/unloading of the Fe-NA complex. Conclusions The results suggest that Si enhances remobilization of Fe from older to younger leaves by a more efficient NA-mediated Fe transport via the phloem. In addition, from this and previous work, a model is proposed of how Si acts to improve Fe homeostasis under Fe deficiency in cucumber.
PB  - Oxford Univ Press, Oxford
T2  - Annals of Botany
T1  - Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions
EP  - 280
IS  - 2
SP  - 271
VL  - 118
DO  - 10.1093/aob/mcw105
ER  - 

@article{
author = "Pavlović, Jelena and Samardžić, Jelena T. and Kostić Kravljanac, Ljiljana and Laursen, Kristian H. and Natic, Maja and Timotijević, Gordana and Schjoerring, Jan K. and Nikolic, Miroslav",
year = "2016",
abstract = "Background and Aims Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe deficiency. Previous work has shown that silicon (Si) can alleviate Fe deficiency by enhancing acquisition and root to shoot translocation of Fe. Here the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe-limiting conditions was investigated. Methods Iron (Fe-57 or naturally occurring isotopes) was measured in leaves at different positions on plants hydroponically growing with or without Si supply. In parallel, the concentration of the Fe chelator nicotianamine (NA) along with the expression of nicotianamine synthase (NAS) involved in its biosynthesis and the expression of yellow stripe-like (YSL) transcripts mediating Fe-NA transport were also determined. Key Results In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. In contrast, Si-treated plants showed an almost even Fe distribution among leaves with four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This Si-stimulated Fe export was paralleled by an increased NA accumulation and expression of the YSL1 transporter for phloem loading/unloading of the Fe-NA complex. Conclusions The results suggest that Si enhances remobilization of Fe from older to younger leaves by a more efficient NA-mediated Fe transport via the phloem. In addition, from this and previous work, a model is proposed of how Si acts to improve Fe homeostasis under Fe deficiency in cucumber.",
publisher = "Oxford Univ Press, Oxford",
journal = "Annals of Botany",
title = "Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions",
pages = "280-271",
number = "2",
volume = "118",
doi = "10.1093/aob/mcw105"
}

Pavlović, J., Samardžić, J. T., Kostić Kravljanac, L., Laursen, K. H., Natic, M., Timotijević, G., Schjoerring, J. K.,& Nikolic, M.. (2016). Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions. in Annals of Botany
Oxford Univ Press, Oxford., 118(2), 271-280.
https://doi.org/10.1093/aob/mcw105

Pavlović J, Samardžić JT, Kostić Kravljanac L, Laursen KH, Natic M, Timotijević G, Schjoerring JK, Nikolic M. Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions. in Annals of Botany. 2016;118(2):271-280.
doi:10.1093/aob/mcw105 .

Pavlović, Jelena, Samardžić, Jelena T., Kostić Kravljanac, Ljiljana, Laursen, Kristian H., Natic, Maja, Timotijević, Gordana, Schjoerring, Jan K., Nikolic, Miroslav, "Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions" in Annals of Botany, 118, no. 2 (2016):271-280,
https://doi.org/10.1093/aob/mcw105 . .

TY  - JOUR
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolić, Nina
AU  - Samardžić, Jelena T.
AU  - Milisavljević, Mira
AU  - Maksimović, Vuk
AU  - Cakmak, Dragan
AU  - Manojlović, Dragan
AU  - Nikolic, Miroslav
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/838
AB  - We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).
PB  - Springer, New York
T2  - Biology and Fertility of Soils
T1  - Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat
EP  - 298
IS  - 3
SP  - 289
VL  - 51
DO  - 10.1007/s00374-014-0975-y
ER  - 

@article{
author = "Kostić Kravljanac, Ljiljana and Nikolić, Nina and Samardžić, Jelena T. and Milisavljević, Mira and Maksimović, Vuk and Cakmak, Dragan and Manojlović, Dragan and Nikolic, Miroslav",
year = "2015",
abstract = "We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).",
publisher = "Springer, New York",
journal = "Biology and Fertility of Soils",
title = "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat",
pages = "298-289",
number = "3",
volume = "51",
doi = "10.1007/s00374-014-0975-y"
}

Kostić Kravljanac, L., Nikolić, N., Samardžić, J. T., Milisavljević, M., Maksimović, V., Cakmak, D., Manojlović, D.,& Nikolic, M.. (2015). Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat. in Biology and Fertility of Soils
Springer, New York., 51(3), 289-298.
https://doi.org/10.1007/s00374-014-0975-y

Kostić Kravljanac L, Nikolić N, Samardžić JT, Milisavljević M, Maksimović V, Cakmak D, Manojlović D, Nikolic M. Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat. in Biology and Fertility of Soils. 2015;51(3):289-298.
doi:10.1007/s00374-014-0975-y .

Kostić Kravljanac, Ljiljana, Nikolić, Nina, Samardžić, Jelena T., Milisavljević, Mira, Maksimović, Vuk, Cakmak, Dragan, Manojlović, Dragan, Nikolic, Miroslav, "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat" in Biology and Fertility of Soils, 51, no. 3 (2015):289-298,
https://doi.org/10.1007/s00374-014-0975-y . .

TY  - JOUR
AU  - Timotijević, Gordana
AU  - Milisavljević, Mira
AU  - Nikolic, Dragana B.
AU  - Milovanović, Bosko M.
AU  - Nikolic, Dragana S.
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/848
AB  - MicroRNAs (miRNAs) belong to the class of small non-coding RNAs which have important roles throughout development as well as in plant response to diverse environmental stresses. Some of plant miRNAs are essential for regulation and maintenance of nutritive homeostasis when nutrients are in excess or shortage comparing to optimal concentration for certain plant species. Better understanding of miRNAs functions implies development of efficient technology for profiling their gene expression. We set out to establish validate the methodology for miRNA gene expression analysis in cucumber grown under suboptimal mineral nutrient regimes, including iron deficiency. Reverse transcription by "stem-loop" primers in combination with Real time PCR method is one of potential approaches for quantification of miRNA gene expression. In this paper we presented a method for "stem loop" primer design specific for miR398, as well as reaction optimization and determination of Real time PCR efficiency. Proving the accuracy of this method was imperative as "stem loop" RT which consider separate transcription of target and endogenous control. The method was verified by comparison of the obtained data with results of miR398 expression achieved using a commercial kit based on simultaneous conversion of all RNAs in cDNAs.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis
EP  - 416
IS  - 2
SP  - 405
VL  - 47
DO  - 10.2298/GENSR1502405T
ER  - 

@article{
author = "Timotijević, Gordana and Milisavljević, Mira and Nikolic, Dragana B. and Milovanović, Bosko M. and Nikolic, Dragana S. and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2015",
abstract = "MicroRNAs (miRNAs) belong to the class of small non-coding RNAs which have important roles throughout development as well as in plant response to diverse environmental stresses. Some of plant miRNAs are essential for regulation and maintenance of nutritive homeostasis when nutrients are in excess or shortage comparing to optimal concentration for certain plant species. Better understanding of miRNAs functions implies development of efficient technology for profiling their gene expression. We set out to establish validate the methodology for miRNA gene expression analysis in cucumber grown under suboptimal mineral nutrient regimes, including iron deficiency. Reverse transcription by "stem-loop" primers in combination with Real time PCR method is one of potential approaches for quantification of miRNA gene expression. In this paper we presented a method for "stem loop" primer design specific for miR398, as well as reaction optimization and determination of Real time PCR efficiency. Proving the accuracy of this method was imperative as "stem loop" RT which consider separate transcription of target and endogenous control. The method was verified by comparison of the obtained data with results of miR398 expression achieved using a commercial kit based on simultaneous conversion of all RNAs in cDNAs.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis",
pages = "416-405",
number = "2",
volume = "47",
doi = "10.2298/GENSR1502405T"
}

Timotijević, G., Milisavljević, M., Nikolic, D. B., Milovanović, B. M., Nikolic, D. S., Nikolic, M.,& Samardžić, J. T.. (2015). Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(2), 405-416.
https://doi.org/10.2298/GENSR1502405T

Timotijević G, Milisavljević M, Nikolic DB, Milovanović BM, Nikolic DS, Nikolic M, Samardžić JT. Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis. in Genetika-Belgrade. 2015;47(2):405-416.
doi:10.2298/GENSR1502405T .

Timotijević, Gordana, Milisavljević, Mira, Nikolic, Dragana B., Milovanović, Bosko M., Nikolic, Dragana S., Nikolic, Miroslav, Samardžić, Jelena T., "Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis" in Genetika-Belgrade, 47, no. 2 (2015):405-416,
https://doi.org/10.2298/GENSR1502405T . .

TY  - JOUR
AU  - Pavlović, Jelena
AU  - Samardžić, Jelena T.
AU  - Maksimović, Vuk
AU  - Timotijević, Gordana
AU  - Stević, Nenad M
AU  - Laursen, Kristian H
AU  - Hansen, Thomas H
AU  - Husted, Soren
AU  - Schjoerring, Jan K
AU  - Liang, Yongchao
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/665
AB  - Root responses to lack of iron (Fe) have mainly been studied in nutrient solution experiments devoid of silicon (Si). Here we investigated how Si ameliorates Fe deficiency in cucumber (Cucumis sativus) with focus on the storage and utilization of Fe in the root apoplast. A combined approach was performed including analyses of apoplastic Fe, reduction-based Fe acquisition and Fe-mobilizing compounds in roots along with the expression of related genes. Si-treated plants accumulated higher concentrations of root apoplastic Fe, which rapidly decreased when Fe was withheld from the nutrient solution. Under Fe-deficient conditions, Si also increased the accumulation of Fe-mobilizing compounds in roots. Si supply stimulated root activity of Fe acquisition at the early stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in Fe acquisition. However, when the period of Fe deprivation was extended, these reactions further decreased as a consequence of Si-induced enhancement of the Fe status of the plants. This work provides new evidence for the beneficial role of Si in plant nutrition and clearly indicates that Si-mediated alleviation of Fe deficiency includes an increase of the apoplastic Fe pool in roots and an enhancement of Fe acquisition.
PB  - Wiley, Hoboken
T2  - New Phytologist
T1  - Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast
EP  - 1107
IS  - 4
SP  - 1096
VL  - 198
DO  - 10.1111/nph.12213
ER  - 

@article{
author = "Pavlović, Jelena and Samardžić, Jelena T. and Maksimović, Vuk and Timotijević, Gordana and Stević, Nenad M and Laursen, Kristian H and Hansen, Thomas H and Husted, Soren and Schjoerring, Jan K and Liang, Yongchao and Nikolic, Miroslav",
year = "2013",
abstract = "Root responses to lack of iron (Fe) have mainly been studied in nutrient solution experiments devoid of silicon (Si). Here we investigated how Si ameliorates Fe deficiency in cucumber (Cucumis sativus) with focus on the storage and utilization of Fe in the root apoplast. A combined approach was performed including analyses of apoplastic Fe, reduction-based Fe acquisition and Fe-mobilizing compounds in roots along with the expression of related genes. Si-treated plants accumulated higher concentrations of root apoplastic Fe, which rapidly decreased when Fe was withheld from the nutrient solution. Under Fe-deficient conditions, Si also increased the accumulation of Fe-mobilizing compounds in roots. Si supply stimulated root activity of Fe acquisition at the early stage of Fe deficiency stress through regulation of gene expression levels of proteins involved in Fe acquisition. However, when the period of Fe deprivation was extended, these reactions further decreased as a consequence of Si-induced enhancement of the Fe status of the plants. This work provides new evidence for the beneficial role of Si in plant nutrition and clearly indicates that Si-mediated alleviation of Fe deficiency includes an increase of the apoplastic Fe pool in roots and an enhancement of Fe acquisition.",
publisher = "Wiley, Hoboken",
journal = "New Phytologist",
title = "Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast",
pages = "1107-1096",
number = "4",
volume = "198",
doi = "10.1111/nph.12213"
}

Pavlović, J., Samardžić, J. T., Maksimović, V., Timotijević, G., Stević, N. M., Laursen, K. H., Hansen, T. H., Husted, S., Schjoerring, J. K., Liang, Y.,& Nikolic, M.. (2013). Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast. in New Phytologist
Wiley, Hoboken., 198(4), 1096-1107.
https://doi.org/10.1111/nph.12213

Pavlović J, Samardžić JT, Maksimović V, Timotijević G, Stević NM, Laursen KH, Hansen TH, Husted S, Schjoerring JK, Liang Y, Nikolic M. Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast. in New Phytologist. 2013;198(4):1096-1107.
doi:10.1111/nph.12213 .

Pavlović, Jelena, Samardžić, Jelena T., Maksimović, Vuk, Timotijević, Gordana, Stević, Nenad M, Laursen, Kristian H, Hansen, Thomas H, Husted, Soren, Schjoerring, Jan K, Liang, Yongchao, Nikolic, Miroslav, "Silicon alleviates iron deficiency in cucumber by promoting mobilization of iron in the root apoplast" in New Phytologist, 198, no. 4 (2013):1096-1107,
https://doi.org/10.1111/nph.12213 . .