TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Đorđević Aleksić, Jelena
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2173
AB  - One of the most severe threats to the Danube River course through Serbia is the direct discharge of untreated wastewater. In biomonitoring studies, faecal indicator bacteria are used to reveal the presence of untreated communal wastewater, while biomarker response in indigenous biota serves as a useful indicator of their harmful potential. The objective of this study was to estimate the genotoxic potential of untreated communal wastewater at two sites on the Danube River, Višnjica (Belgrade) and Novi Banovci, by using in situ approach in white bream (Blicca bjoerkna L.). A comet assay was selected to measure DNA damage in erythrocytes, liver, and gill cells, while the micronucleus test was applied to measure chromosomal aberrations in erythrocytes.
Additionally, the accumulation of 22 elements in fish liver and muscle was analysed by ICP-OES meth- od. Simultaneous detection of total coliforms and E.coli in water was performed by enzyme-based Colilert-18 test (IDEXX). Comet assay revealed the highest level of DNA damage in gills at the site Novi Banovci, and in blood at the site Višnjica. The overall frequency of micronucleus at both sites was low. At both localities, the majority of analysed elements showed higher levels in the liver in comparison to muscle. Microbiological indicators confirmed the poor water quality, since at both sites water was categorised as critically to strongly polluted. The results of this study highlighted the importance of in situ biomonitoring approach and the need for more effective management of natural resources and implementation of wastewater treatment systems.
PB  - Serbian Society of Toxicology
C3  - 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
T1  - Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2173
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Sunjog, Karolina and Kolarević, Stoimir and Višnjić Jeftić, Željka and Subotić, Srđan and Đorđević Aleksić, Jelena and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2023",
abstract = "One of the most severe threats to the Danube River course through Serbia is the direct discharge of untreated wastewater. In biomonitoring studies, faecal indicator bacteria are used to reveal the presence of untreated communal wastewater, while biomarker response in indigenous biota serves as a useful indicator of their harmful potential. The objective of this study was to estimate the genotoxic potential of untreated communal wastewater at two sites on the Danube River, Višnjica (Belgrade) and Novi Banovci, by using in situ approach in white bream (Blicca bjoerkna L.). A comet assay was selected to measure DNA damage in erythrocytes, liver, and gill cells, while the micronucleus test was applied to measure chromosomal aberrations in erythrocytes.
Additionally, the accumulation of 22 elements in fish liver and muscle was analysed by ICP-OES meth- od. Simultaneous detection of total coliforms and E.coli in water was performed by enzyme-based Colilert-18 test (IDEXX). Comet assay revealed the highest level of DNA damage in gills at the site Novi Banovci, and in blood at the site Višnjica. The overall frequency of micronucleus at both sites was low. At both localities, the majority of analysed elements showed higher levels in the liver in comparison to muscle. Microbiological indicators confirmed the poor water quality, since at both sites water was categorised as critically to strongly polluted. The results of this study highlighted the importance of in situ biomonitoring approach and the need for more effective management of natural resources and implementation of wastewater treatment systems.",
publisher = "Serbian Society of Toxicology",
journal = "13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia",
title = "Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2173"
}

Kostić-Vuković, J., Sunjog, K., Kolarević, S., Višnjić Jeftić, Ž., Subotić, S., Đorđević Aleksić, J., Lenhardt, M.,& Vuković-Gačić, B.. (2023). Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.. in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
Serbian Society of Toxicology., 72.
https://hdl.handle.net/21.15107/rcub_rimsi_2173

Kostić-Vuković J, Sunjog K, Kolarević S, Višnjić Jeftić Ž, Subotić S, Đorđević Aleksić J, Lenhardt M, Vuković-Gačić B. Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.. in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia. 2023;:72.
https://hdl.handle.net/21.15107/rcub_rimsi_2173 .

Kostić-Vuković, Jovana, Sunjog, Karolina, Kolarević, Stoimir, Višnjić Jeftić, Željka, Subotić, Srđan, Đorđević Aleksić, Jelena, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment." in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia (2023):72,
https://hdl.handle.net/21.15107/rcub_rimsi_2173 .

TY  - CONF
AU  - Spasić, Slađana
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Lenhardt, Mirjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1663
AB  - Fish erythrocytes released from erythropoietic sites are immature. During circulation they increase their own surface while the cell body and nuclei begin to become more elliptical and less spherical during maturaration. The relative abundance of different developmental stages represents an erythron profile which could be a more sensitive indicator of contamination than classic hematological indices. In this study, we analyzed blood smears of the Common Nase (Chodrostoma nasus) with the main goal to identify the determinants of the developmental stage of erythrocytes. Based on parameters developmental stages, erythrocytes are categorized into immature, intermediate or mature. In this investigation we have used four classification methods: the Two Step Cluster analysis, the K-Means Cluster analysis and Neural Neworks - Multilayer Perceptron and an Ordinal Regression Model. Our findings clearly justify that Multilayers Perceptron and ORL models are appropriate to classify the developmental stage of fish erythrocytes.
PB  - Belarusian State University of lnformatics and Radioelectronics
C3  - 14th International Conference on Pattern Recognition and Information Processing – PRIP2019, Minsk, Belarus
T1  - Determination of the development stage of erythrocytes in the common nase (Chondrostoma nasus) using different classification methods.
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1663
ER  - 

@conference{
author = "Spasić, Slađana and Subotić, Srđan and Višnjić Jeftić, Željka and Lenhardt, Mirjana",
year = "2019",
abstract = "Fish erythrocytes released from erythropoietic sites are immature. During circulation they increase their own surface while the cell body and nuclei begin to become more elliptical and less spherical during maturaration. The relative abundance of different developmental stages represents an erythron profile which could be a more sensitive indicator of contamination than classic hematological indices. In this study, we analyzed blood smears of the Common Nase (Chodrostoma nasus) with the main goal to identify the determinants of the developmental stage of erythrocytes. Based on parameters developmental stages, erythrocytes are categorized into immature, intermediate or mature. In this investigation we have used four classification methods: the Two Step Cluster analysis, the K-Means Cluster analysis and Neural Neworks - Multilayer Perceptron and an Ordinal Regression Model. Our findings clearly justify that Multilayers Perceptron and ORL models are appropriate to classify the developmental stage of fish erythrocytes.",
publisher = "Belarusian State University of lnformatics and Radioelectronics",
journal = "14th International Conference on Pattern Recognition and Information Processing – PRIP2019, Minsk, Belarus",
title = "Determination of the development stage of erythrocytes in the common nase (Chondrostoma nasus) using different classification methods.",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1663"
}

Spasić, S., Subotić, S., Višnjić Jeftić, Ž.,& Lenhardt, M.. (2019). Determination of the development stage of erythrocytes in the common nase (Chondrostoma nasus) using different classification methods.. in 14th International Conference on Pattern Recognition and Information Processing – PRIP2019, Minsk, Belarus
Belarusian State University of lnformatics and Radioelectronics..
https://hdl.handle.net/21.15107/rcub_rimsi_1663

Spasić S, Subotić S, Višnjić Jeftić Ž, Lenhardt M. Determination of the development stage of erythrocytes in the common nase (Chondrostoma nasus) using different classification methods.. in 14th International Conference on Pattern Recognition and Information Processing – PRIP2019, Minsk, Belarus. 2019;.
https://hdl.handle.net/21.15107/rcub_rimsi_1663 .

Spasić, Slađana, Subotić, Srđan, Višnjić Jeftić, Željka, Lenhardt, Mirjana, "Determination of the development stage of erythrocytes in the common nase (Chondrostoma nasus) using different classification methods." in 14th International Conference on Pattern Recognition and Information Processing – PRIP2019, Minsk, Belarus (2019),
https://hdl.handle.net/21.15107/rcub_rimsi_1663 .

TY  - CONF
AU  - Krpo Ćetković, Jasmina
AU  - Prica, Milijana
AU  - Subotić, Srđan
AU  - Nikčević, Miroslav
AU  - Mićković, Branislav
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2440
PB  - Geomorphologia Slovaca et Bohemica
C3  - Geomorphologia Slovaca et Bohemica
T1  - Lenght-weight relationship and condition of three goby species in the Danube River near Slankamen (Serbia)
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2440
ER  - 

@conference{
author = "Krpo Ćetković, Jasmina and Prica, Milijana and Subotić, Srđan and Nikčević, Miroslav and Mićković, Branislav",
year = "2018",
publisher = "Geomorphologia Slovaca et Bohemica",
journal = "Geomorphologia Slovaca et Bohemica",
title = "Lenght-weight relationship and condition of three goby species in the Danube River near Slankamen (Serbia)",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2440"
}

Krpo Ćetković, J., Prica, M., Subotić, S., Nikčević, M.,& Mićković, B.. (2018). Lenght-weight relationship and condition of three goby species in the Danube River near Slankamen (Serbia). in Geomorphologia Slovaca et Bohemica
Geomorphologia Slovaca et Bohemica..
https://hdl.handle.net/21.15107/rcub_rimsi_2440

Krpo Ćetković J, Prica M, Subotić S, Nikčević M, Mićković B. Lenght-weight relationship and condition of three goby species in the Danube River near Slankamen (Serbia). in Geomorphologia Slovaca et Bohemica. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2440 .

Krpo Ćetković, Jasmina, Prica, Milijana, Subotić, Srđan, Nikčević, Miroslav, Mićković, Branislav, "Lenght-weight relationship and condition of three goby species in the Danube River near Slankamen (Serbia)" in Geomorphologia Slovaca et Bohemica (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2440 .

TY  - CONF
AU  - Lenhardt, Mirjana
AU  - Spasić, Slađana
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Hegediš, Aleksandar
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2387
PB  - German National Committee for the International Hydrological Programme of UNESCO and for the Hydrology and Water Resources Programm of WMO Bavarian Environment Agency Deggendorf Institute of Technology, Nemačka
C3  - XXVIConference of the Danube Countries on Hydrological Forecasting and Hydrological Bases of Water Management
T1  - Analysis of 18 elements in liver and gills of three Danubian predatory fish species
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2387
ER  - 

@conference{
author = "Lenhardt, Mirjana and Spasić, Slađana and Višnjić Jeftić, Željka and Subotić, Srđan and Hegediš, Aleksandar",
year = "2014",
publisher = "German National Committee for the International Hydrological Programme of UNESCO and for the Hydrology and Water Resources Programm of WMO Bavarian Environment Agency Deggendorf Institute of Technology, Nemačka",
journal = "XXVIConference of the Danube Countries on Hydrological Forecasting and Hydrological Bases of Water Management",
title = "Analysis of 18 elements in liver and gills of three Danubian predatory fish species",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2387"
}

Lenhardt, M., Spasić, S., Višnjić Jeftić, Ž., Subotić, S.,& Hegediš, A.. (2014). Analysis of 18 elements in liver and gills of three Danubian predatory fish species. in XXVIConference of the Danube Countries on Hydrological Forecasting and Hydrological Bases of Water Management
German National Committee for the International Hydrological Programme of UNESCO and for the Hydrology and Water Resources Programm of WMO Bavarian Environment Agency Deggendorf Institute of Technology, Nemačka..
https://hdl.handle.net/21.15107/rcub_rimsi_2387

Lenhardt M, Spasić S, Višnjić Jeftić Ž, Subotić S, Hegediš A. Analysis of 18 elements in liver and gills of three Danubian predatory fish species. in XXVIConference of the Danube Countries on Hydrological Forecasting and Hydrological Bases of Water Management. 2014;.
https://hdl.handle.net/21.15107/rcub_rimsi_2387 .

Lenhardt, Mirjana, Spasić, Slađana, Višnjić Jeftić, Željka, Subotić, Srđan, Hegediš, Aleksandar, "Analysis of 18 elements in liver and gills of three Danubian predatory fish species" in XXVIConference of the Danube Countries on Hydrological Forecasting and Hydrological Bases of Water Management (2014),
https://hdl.handle.net/21.15107/rcub_rimsi_2387 .