TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1951
AB  - BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown 
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among 
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause 
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic 
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids 
(Cheers F1 and Hippo F1) grown in Futog in 2021. 
METHODS 
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and 
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed 
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed 
with concatenated sequences.
RESULTS 
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a 
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head 
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as 
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present 
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were 
separated from P. carotovorum isolates, clustered into four groups.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology
T1  - PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)
SP  - 915
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1951
ER  - 

@conference{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
abstract = "BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown 
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among 
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause 
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic 
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids 
(Cheers F1 and Hippo F1) grown in Futog in 2021. 
METHODS 
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and 
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed 
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed 
with concatenated sequences.
RESULTS 
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a 
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head 
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as 
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present 
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were 
separated from P. carotovorum isolates, clustered into four groups.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology",
title = "PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)",
pages = "915",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1951"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Stanković, S.,& Popović, T.. (2022). PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 915.
https://hdl.handle.net/21.15107/rcub_rimsi_1951

Jelušić A, Mitrović P, Marković S, Iličić R, Stanković S, Popović T. PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:915.
https://hdl.handle.net/21.15107/rcub_rimsi_1951 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Stanković, Slaviša, Popović, Tatjana, "PATHOGENIC BACTERIAL POPULATION CAUSING SOFT  ROT ON CABBAGE: CASE STUDY IN FUTOG (SERBIA)" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology (2022):915,
https://hdl.handle.net/21.15107/rcub_rimsi_1951 .