TY  - CONF
AU  - Marković, Sanja
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1931
AB  - Background: Pectolytic Dickeya and Pectobacterium species that cause blackleg, aerial stem rot and 
soft rot are commercially important pathogens in potato production. Blackleg symptom caused by 
these bacteria has been recorded in potato production field in Bačka region (Vojvodina, Serbia) in 
2018. 
Objectives: Metagenomic approach was used to reveal microbial communities colonizing the 
diseased potato tubers with the aim of better understanding of pathogens distribution.
Methods: Single colonies of potential pathogenic bacteria were isolated on nutrient agar and 
checked for pectolytic activity on potato slices. Ten selected bacterial isolates were identified to the 
species level after PCR amplification with gapA and mdh primers. The 16S rRNA sequences from 
diseased potato tubers samples were obtained using Next generation sequencing (NGS) Illumina 
platform. The obtained sequencing data was processed bioinformaticaly.
Results: Mixed infection of the diseased potato plants with Dickeya dianthocola and Pectobacterium 
carotovorum pv. brasiliensis was revealed after pathogen identification with gapA and mdh genes. All 
isolates caused soft rot symptoms on potato slices. Metagenomic analysis revealed that present 
bacteria belong to phylum Bacteroidetes (Bacteroides, Empedobacter, Dysgonomonas, Myroides, and 
ud-Porphyromonadaceae), Firmicutes (Vagococcus, Lactococcus, Clostridium XIVa, Enterococcus, and 
ud-Acidaminococcaceae) and Proteobacteria (Acinetobacter, ud-Pseudomonadaceae, ud Enterobacteriaceae, Wohlfahrtiimonas, ud-Neisseriaceae, Enterobacter, Providencia, Arcobacter, and 
Kerstersia) as the most common representives. Genus Pectobacterium was present in 0.5%.
PB  - Federation of European Microbiological Societies
C3  - 8th Congress of  European Microbiologists
T1  - Metagenomic analysis of microbial communities associated with diseased potato tubers
SP  - 881
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1931
ER  - 

@conference{
author = "Marković, Sanja and Dimkić, Ivica and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Popović, Tatjana",
year = "2019",
abstract = "Background: Pectolytic Dickeya and Pectobacterium species that cause blackleg, aerial stem rot and 
soft rot are commercially important pathogens in potato production. Blackleg symptom caused by 
these bacteria has been recorded in potato production field in Bačka region (Vojvodina, Serbia) in 
2018. 
Objectives: Metagenomic approach was used to reveal microbial communities colonizing the 
diseased potato tubers with the aim of better understanding of pathogens distribution.
Methods: Single colonies of potential pathogenic bacteria were isolated on nutrient agar and 
checked for pectolytic activity on potato slices. Ten selected bacterial isolates were identified to the 
species level after PCR amplification with gapA and mdh primers. The 16S rRNA sequences from 
diseased potato tubers samples were obtained using Next generation sequencing (NGS) Illumina 
platform. The obtained sequencing data was processed bioinformaticaly.
Results: Mixed infection of the diseased potato plants with Dickeya dianthocola and Pectobacterium 
carotovorum pv. brasiliensis was revealed after pathogen identification with gapA and mdh genes. All 
isolates caused soft rot symptoms on potato slices. Metagenomic analysis revealed that present 
bacteria belong to phylum Bacteroidetes (Bacteroides, Empedobacter, Dysgonomonas, Myroides, and 
ud-Porphyromonadaceae), Firmicutes (Vagococcus, Lactococcus, Clostridium XIVa, Enterococcus, and 
ud-Acidaminococcaceae) and Proteobacteria (Acinetobacter, ud-Pseudomonadaceae, ud Enterobacteriaceae, Wohlfahrtiimonas, ud-Neisseriaceae, Enterobacter, Providencia, Arcobacter, and 
Kerstersia) as the most common representives. Genus Pectobacterium was present in 0.5%.",
publisher = "Federation of European Microbiological Societies",
journal = "8th Congress of  European Microbiologists",
title = "Metagenomic analysis of microbial communities associated with diseased potato tubers",
pages = "881",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1931"
}

Marković, S., Dimkić, I., Stanković, S., Jelušić, A., Iličić, R.,& Popović, T.. (2019). Metagenomic analysis of microbial communities associated with diseased potato tubers. in 8th Congress of  European Microbiologists
Federation of European Microbiological Societies., 881.
https://hdl.handle.net/21.15107/rcub_rimsi_1931

Marković S, Dimkić I, Stanković S, Jelušić A, Iličić R, Popović T. Metagenomic analysis of microbial communities associated with diseased potato tubers. in 8th Congress of  European Microbiologists. 2019;:881.
https://hdl.handle.net/21.15107/rcub_rimsi_1931 .

Marković, Sanja, Dimkić, Ivica, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Popović, Tatjana, "Metagenomic analysis of microbial communities associated with diseased potato tubers" in 8th Congress of  European Microbiologists (2019):881,
https://hdl.handle.net/21.15107/rcub_rimsi_1931 .