TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1935
AB  - Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or 
endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere. 
Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight 
symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae
vegetables.
Objectives: Metagenomic approach was used to reveal differences in microbial communities 
colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial
blight symptoms and one healthy variety, all collected from five different localities in Vojvodina 
(Serbia) in 2018. 
Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA
sequences from five tested samples, and to gain insight into their alpha and beta diversity. 
Comparative taxonomic analysis of the obtained sequencing metadata was processed 
bioinformaticaly.
Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria, 
Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes. 
Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike 
healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the 
most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were 
present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in 
healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then 
1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae) 
in healthy oilseed rape variety.
PB  - Federation of European Microbiological Societies
C3  - 8th Congress of  European Microbiologists
T1  - Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape
SP  - 899
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1935
ER  - 

@conference{
author = "Jelušić, Aleksandra and Dimkić, Ivica and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana",
year = "2019",
abstract = "Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or 
endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere. 
Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight 
symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae
vegetables.
Objectives: Metagenomic approach was used to reveal differences in microbial communities 
colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial
blight symptoms and one healthy variety, all collected from five different localities in Vojvodina 
(Serbia) in 2018. 
Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA
sequences from five tested samples, and to gain insight into their alpha and beta diversity. 
Comparative taxonomic analysis of the obtained sequencing metadata was processed 
bioinformaticaly.
Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria, 
Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes. 
Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike 
healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the 
most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were 
present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in 
healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then 
1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae) 
in healthy oilseed rape variety.",
publisher = "Federation of European Microbiological Societies",
journal = "8th Congress of  European Microbiologists",
title = "Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape",
pages = "899",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1935"
}

Jelušić, A., Dimkić, I., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape. in 8th Congress of  European Microbiologists
Federation of European Microbiological Societies., 899.
https://hdl.handle.net/21.15107/rcub_rimsi_1935

Jelušić A, Dimkić I, Berić T, Mitrović P, Marković S, Stanković S, Popović T. Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape. in 8th Congress of  European Microbiologists. 2019;:899.
https://hdl.handle.net/21.15107/rcub_rimsi_1935 .

Jelušić, Aleksandra, Dimkić, Ivica, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape" in 8th Congress of  European Microbiologists (2019):899,
https://hdl.handle.net/21.15107/rcub_rimsi_1935 .