Dimkić, Ivica

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  • Dimkić, Ivica (1)
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IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP. AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA

Jelušić, Aleksandra; Berić, Tanja; Dimkić, Ivica; Mitrović, Petar; Stanković, Slaviša; Marković, Sanja; Popović, Tatjana

(Federation of European Microbiological Societies in association with the Serbian Society of Microbiology, 2020)

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Popović, Tatjana
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1950
AB  - Background:
Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium 
Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as
a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression.
Objectives:
With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, 
this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like
isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants.
Methods:
Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free 
supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape 
Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the 
sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced.
Results:
Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. 
cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. 
megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology
T1  - IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1950
ER  - 
@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Popović, Tatjana",
year = "2020",
abstract = "Background:
Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium 
Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as
a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression.
Objectives:
With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, 
this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like
isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants.
Methods:
Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free 
supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape 
Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the 
sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced.
Results:
Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. 
cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. 
megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology",
title = "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA",
pages = "133",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1950"
}
Jelušić, A., Berić, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Popović, T.. (2020). IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 133.
https://hdl.handle.net/21.15107/rcub_rimsi_1950
Jelušić A, Berić T, Dimkić I, Mitrović P, Stanković S, Marković S, Popović T. IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology. 2020;:133.
https://hdl.handle.net/21.15107/rcub_rimsi_1950 .
Jelušić, Aleksandra, Berić, Tanja, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Popović, Tatjana, "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP.  AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA" in FEMS Online Conference on Microbiology (2020):133,
https://hdl.handle.net/21.15107/rcub_rimsi_1950 .