TY  - JOUR
AU  - Marković, Sanja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Ilicic, Renata
AU  - Bagi, Ferenc
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1542
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia
IS  - 1
VL  - 106
DO  - 10.1094/PDIS-06-21-1128-PDN
ER  - 

@article{
author = "Marković, Sanja and Milić Komić, Sonja and Jelušić, Aleksandra and Ilicic, Renata and Bagi, Ferenc and Stanković, Slaviša and Popović, Tatjana",
year = "2022",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia",
number = "1",
volume = "106",
doi = "10.1094/PDIS-06-21-1128-PDN"
}

Marković, S., Milić Komić, S., Jelušić, A., Ilicic, R., Bagi, F., Stanković, S.,& Popović, T.. (2022). First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 106(1).
https://doi.org/10.1094/PDIS-06-21-1128-PDN

Marković S, Milić Komić S, Jelušić A, Ilicic R, Bagi F, Stanković S, Popović T. First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia. in Plant Disease. 2022;106(1).
doi:10.1094/PDIS-06-21-1128-PDN .

Marković, Sanja, Milić Komić, Sonja, Jelušić, Aleksandra, Ilicic, Renata, Bagi, Ferenc, Stanković, Slaviša, Popović, Tatjana, "First Report of Pectobacterium versatile Causing Blackleg of Potato in Serbia" in Plant Disease, 106, no. 1 (2022),
https://doi.org/10.1094/PDIS-06-21-1128-PDN . .

TY  - JOUR
AU  - Ilicic, Renata
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Barac, Goran
AU  - Bagi, Ferenc
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1531
AB  - In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Sancevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes-gapA, gltA, rpoD and gyrB-showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305(T) (=58(T) = LMG28609(T)) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +- - - + (Pseudomonas Group Ia) and GATTa + - + -, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification
EP  - 150
IS  - 1
SP  - 140
VL  - 180
DO  - 10.1111/aab.12717
ER  - 

@article{
author = "Ilicic, Renata and Jelušić, Aleksandra and Marković, Sanja and Barac, Goran and Bagi, Ferenc and Popović, Tatjana",
year = "2022",
abstract = "In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Sancevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes-gapA, gltA, rpoD and gyrB-showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305(T) (=58(T) = LMG28609(T)) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +- - - + (Pseudomonas Group Ia) and GATTa + - + -, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification",
pages = "150-140",
number = "1",
volume = "180",
doi = "10.1111/aab.12717"
}

Ilicic, R., Jelušić, A., Marković, S., Barac, G., Bagi, F.,& Popović, T.. (2022). Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology
Wiley, Hoboken., 180(1), 140-150.
https://doi.org/10.1111/aab.12717

Ilicic R, Jelušić A, Marković S, Barac G, Bagi F, Popović T. Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology. 2022;180(1):140-150.
doi:10.1111/aab.12717 .

Ilicic, Renata, Jelušić, Aleksandra, Marković, Sanja, Barac, Goran, Bagi, Ferenc, Popović, Tatjana, "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification" in Annals of Applied Biology, 180, no. 1 (2022):140-150,
https://doi.org/10.1111/aab.12717 . .

TY  - JOUR
AU  - Ilicic, Renata
AU  - Popović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Bagi, Ferenc
AU  - Vlajic, Slobodan
AU  - Stanković, Slaviša
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1419
AB  - Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia
EP  - 262
IS  - 1
SP  - 247
VL  - 53
DO  - 10.2298/GENSR2101247I
ER  - 

@article{
author = "Ilicic, Renata and Popović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Bagi, Ferenc and Vlajic, Slobodan and Stanković, Slaviša",
year = "2021",
abstract = "Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia",
pages = "262-247",
number = "1",
volume = "53",
doi = "10.2298/GENSR2101247I"
}

Ilicic, R., Popović, T., Marković, S., Jelušić, A., Bagi, F., Vlajic, S.,& Stanković, S.. (2021). Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 53(1), 247-262.
https://doi.org/10.2298/GENSR2101247I

Ilicic R, Popović T, Marković S, Jelušić A, Bagi F, Vlajic S, Stanković S. Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika-Belgrade. 2021;53(1):247-262.
doi:10.2298/GENSR2101247I .

Ilicic, Renata, Popović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Bagi, Ferenc, Vlajic, Slobodan, Stanković, Slaviša, "Genetic diversity of pseudomonas syringae pv. Syringae isolated from sweet cherry in southern and northern regions in Serbia" in Genetika-Belgrade, 53, no. 1 (2021):247-262,
https://doi.org/10.2298/GENSR2101247I . .