Pekarik, Ladislav

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  • Pekarik, Ladislav (3)
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Author's Bibliography

Sturgeons in large rivers: detecting the near‑extinct needles in a haystack via eDNA metabarcoding from water samples

Meulenbroek, Paul; Hein, Thomas; Friedrich, Thomas; Valentini, Alice; Erős, Tibor; Schabuss, Michael; Zornig, Horst; Lenhardt, Mirjana; Pekarik, Ladislav; Jean, Pauline; Dejean, Tony; Pont, Didier

(Springer, 2022)

TY  - JOUR
AU  - Meulenbroek, Paul
AU  - Hein, Thomas
AU  - Friedrich, Thomas
AU  - Valentini, Alice
AU  - Erős, Tibor
AU  - Schabuss, Michael
AU  - Zornig, Horst
AU  - Lenhardt, Mirjana
AU  - Pekarik, Ladislav
AU  - Jean, Pauline
AU  - Dejean, Tony
AU  - Pont, Didier
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1557
AB  - Sturgeon populations are declining worldwide and are the target of extensive conservation
efforts. Addressed in several pieces of legislation, sturgeons have received considerable
attention as flagship or umbrella species. Despite the need for a better understanding
of the distribution and population status, the use of traditional sampling methods failed
in the past, thereby hampering reliable assessments, a prerequisite for conservation. Here,
we describe the development and application of an environmental DNA (eDNA) metabarcoding
approach for detecting rare sturgeons in large rivers. Exemplarily, we developed
a reference database for five native Danube sturgeons (Acipenser stellatus, Acipenser
gueldenstaedtii, Acipenser ruthenus, Acipenser nudiventris, and Huso huso) and two nonnative
species (Acipenser baerii and Acipenser transmontanus), assessed these ex situ, and
used eDNA as a detection tool along the entire length of the Danube (Europe, ~ 2850 km)
and major tributaries. In ex situ analyses, all assays yielded positive amplifications for the
assessed sturgeon species. In the Danube, the presence of A. ruthenus was confirmed at 14
of 29 sites (48.3%), and in 2 of 18 tributary sites (11.1%), providing the first comprehensive
large-scale biogeographical snapshot of this species. Relative number of reads assigned to
A. ruthenus varied between 0 and 2.5%, with sites registering positive detections being
clustered in 3 sections of the Danube. Our findings enabled us to confirm the advantages of
eDNA monitoring over traditional sampling methods for comprehensive whole-river snapshot
studies of sturgeons conducted on a large geographical scale, and therefore we consider
it to be a promising approach for application in conservation measures, fisheries management,
scientific studies, and adaptive management plans for sturgeons on a global scale.
PB  - Springer
T2  - Biodiversity and Conservation
T1  - Sturgeons in large rivers: detecting the near‑extinct needles in a haystack via eDNA metabarcoding from water samples
DO  - 10.1007/s10531-022-02459-w
ER  - 
@article{
author = "Meulenbroek, Paul and Hein, Thomas and Friedrich, Thomas and Valentini, Alice and Erős, Tibor and Schabuss, Michael and Zornig, Horst and Lenhardt, Mirjana and Pekarik, Ladislav and Jean, Pauline and Dejean, Tony and Pont, Didier",
year = "2022",
abstract = "Sturgeon populations are declining worldwide and are the target of extensive conservation
efforts. Addressed in several pieces of legislation, sturgeons have received considerable
attention as flagship or umbrella species. Despite the need for a better understanding
of the distribution and population status, the use of traditional sampling methods failed
in the past, thereby hampering reliable assessments, a prerequisite for conservation. Here,
we describe the development and application of an environmental DNA (eDNA) metabarcoding
approach for detecting rare sturgeons in large rivers. Exemplarily, we developed
a reference database for five native Danube sturgeons (Acipenser stellatus, Acipenser
gueldenstaedtii, Acipenser ruthenus, Acipenser nudiventris, and Huso huso) and two nonnative
species (Acipenser baerii and Acipenser transmontanus), assessed these ex situ, and
used eDNA as a detection tool along the entire length of the Danube (Europe, ~ 2850 km)
and major tributaries. In ex situ analyses, all assays yielded positive amplifications for the
assessed sturgeon species. In the Danube, the presence of A. ruthenus was confirmed at 14
of 29 sites (48.3%), and in 2 of 18 tributary sites (11.1%), providing the first comprehensive
large-scale biogeographical snapshot of this species. Relative number of reads assigned to
A. ruthenus varied between 0 and 2.5%, with sites registering positive detections being
clustered in 3 sections of the Danube. Our findings enabled us to confirm the advantages of
eDNA monitoring over traditional sampling methods for comprehensive whole-river snapshot
studies of sturgeons conducted on a large geographical scale, and therefore we consider
it to be a promising approach for application in conservation measures, fisheries management,
scientific studies, and adaptive management plans for sturgeons on a global scale.",
publisher = "Springer",
journal = "Biodiversity and Conservation",
title = "Sturgeons in large rivers: detecting the near‑extinct needles in a haystack via eDNA metabarcoding from water samples",
doi = "10.1007/s10531-022-02459-w"
}
Meulenbroek, P., Hein, T., Friedrich, T., Valentini, A., Erős, T., Schabuss, M., Zornig, H., Lenhardt, M., Pekarik, L., Jean, P., Dejean, T.,& Pont, D.. (2022). Sturgeons in large rivers: detecting the near‑extinct needles in a haystack via eDNA metabarcoding from water samples. in Biodiversity and Conservation
Springer..
https://doi.org/10.1007/s10531-022-02459-w
Meulenbroek P, Hein T, Friedrich T, Valentini A, Erős T, Schabuss M, Zornig H, Lenhardt M, Pekarik L, Jean P, Dejean T, Pont D. Sturgeons in large rivers: detecting the near‑extinct needles in a haystack via eDNA metabarcoding from water samples. in Biodiversity and Conservation. 2022;.
doi:10.1007/s10531-022-02459-w .
Meulenbroek, Paul, Hein, Thomas, Friedrich, Thomas, Valentini, Alice, Erős, Tibor, Schabuss, Michael, Zornig, Horst, Lenhardt, Mirjana, Pekarik, Ladislav, Jean, Pauline, Dejean, Tony, Pont, Didier, "Sturgeons in large rivers: detecting the near‑extinct needles in a haystack via eDNA metabarcoding from water samples" in Biodiversity and Conservation (2022),
https://doi.org/10.1007/s10531-022-02459-w . .
9
5

Influence of the diel period and different sampling methods on catch of gobiids at four locations in the inshore part of the Danube River

Lenhardt, Mirjana; Pekarik, Ladislav; Skorić, Stefan; Smederevac-Lalić, Marija; Hegediš, Aleksandar; Jaćimović, Milica; Đikanović, Vesna

(Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences (IBER-BAS); East and South European Network for Invasive Alien Species (ESENIAS), Institute of Biodiversity and Ecosystem Research Bulgarian Academy of Sciences, 2017)

TY  - CONF
AU  - Lenhardt, Mirjana
AU  - Pekarik, Ladislav
AU  - Skorić, Stefan
AU  - Smederevac-Lalić, Marija
AU  - Hegediš, Aleksandar
AU  - Jaćimović, Milica
AU  - Đikanović, Vesna
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1727
AB  - Some recent data show that five gobiid species have spread outside their native range and
expanded their distribution area in Serbia along the rivers Danube, Tisza, Sava,and Velika
Morava. These are: monkey goby Neogobius fluviatilis, racer goby Neogobius
gymnotrachelus, bighead goby Neogobius kessleri,round goby Neogobius melanostomus,
and tubenose goby Proterorhinus semilunaris.
The aim of this study was to compare the efficiency of two different methods for
fish sampling, as well as to assess influence of the diel period on catch of gobiids in the
Serbian stretch of the Danube River. During October 2012 and September 2013, the
sampling was performed by electrofishing and beach seining in four riverside locations of
the Danube River,as follows: at NoviSad (river km 1257), Belgrade(river km 1173),Tekija
(river km 956),and Prahovo (river km 862),with the water depth being of up to120cm. At
each location,the sampling was performed at 17:30,18:30,19:30 and 20:30.
A total of 551 gobies were caught. The highest number of specimens (238) was registered at Tekija,followed by the sites at Belgrade(192) and Prahovo (109), and only 12 specimens caught at NoviSad.The catch of the round goby and monkey goby was more efficient by beach seining than by electrofishing, while the catch of the tubenose goby was more efficient by electrofishing. Both methods had similar efficiency in terms of the catch of the bighead goby and racer goby.The species diversity and the number of caught specimens were the highest at 18:30.All five species were recorded at the sites of Belgrade,Tekija and Prahovo,while only the monkey goby and racer goby were caught at NoviSad.
The methods used in this study showed good efficiency in catch of gobiids, especially at
dusk. Despite the difficulty in using these methods in certain habitats, we assume that they
could be highly relevant in the regular monitoring of gobiids along the inshore parts of the
rivers.
PB  - Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences (IBER-BAS); East and South European Network for Invasive Alien Species (ESENIAS), Institute of Biodiversity and Ecosystem Research Bulgarian Academy of Sciences
C3  - 7th ESENIAS Workshop with Scientific Conference
T1  - Influence of the diel period and different sampling methods on catch of gobiids at four locations in the inshore part of the Danube River
SP  - 157
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1727
ER  - 
@conference{
author = "Lenhardt, Mirjana and Pekarik, Ladislav and Skorić, Stefan and Smederevac-Lalić, Marija and Hegediš, Aleksandar and Jaćimović, Milica and Đikanović, Vesna",
year = "2017",
abstract = "Some recent data show that five gobiid species have spread outside their native range and
expanded their distribution area in Serbia along the rivers Danube, Tisza, Sava,and Velika
Morava. These are: monkey goby Neogobius fluviatilis, racer goby Neogobius
gymnotrachelus, bighead goby Neogobius kessleri,round goby Neogobius melanostomus,
and tubenose goby Proterorhinus semilunaris.
The aim of this study was to compare the efficiency of two different methods for
fish sampling, as well as to assess influence of the diel period on catch of gobiids in the
Serbian stretch of the Danube River. During October 2012 and September 2013, the
sampling was performed by electrofishing and beach seining in four riverside locations of
the Danube River,as follows: at NoviSad (river km 1257), Belgrade(river km 1173),Tekija
(river km 956),and Prahovo (river km 862),with the water depth being of up to120cm. At
each location,the sampling was performed at 17:30,18:30,19:30 and 20:30.
A total of 551 gobies were caught. The highest number of specimens (238) was registered at Tekija,followed by the sites at Belgrade(192) and Prahovo (109), and only 12 specimens caught at NoviSad.The catch of the round goby and monkey goby was more efficient by beach seining than by electrofishing, while the catch of the tubenose goby was more efficient by electrofishing. Both methods had similar efficiency in terms of the catch of the bighead goby and racer goby.The species diversity and the number of caught specimens were the highest at 18:30.All five species were recorded at the sites of Belgrade,Tekija and Prahovo,while only the monkey goby and racer goby were caught at NoviSad.
The methods used in this study showed good efficiency in catch of gobiids, especially at
dusk. Despite the difficulty in using these methods in certain habitats, we assume that they
could be highly relevant in the regular monitoring of gobiids along the inshore parts of the
rivers.",
publisher = "Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences (IBER-BAS); East and South European Network for Invasive Alien Species (ESENIAS), Institute of Biodiversity and Ecosystem Research Bulgarian Academy of Sciences",
journal = "7th ESENIAS Workshop with Scientific Conference",
title = "Influence of the diel period and different sampling methods on catch of gobiids at four locations in the inshore part of the Danube River",
pages = "157",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1727"
}
Lenhardt, M., Pekarik, L., Skorić, S., Smederevac-Lalić, M., Hegediš, A., Jaćimović, M.,& Đikanović, V.. (2017). Influence of the diel period and different sampling methods on catch of gobiids at four locations in the inshore part of the Danube River. in 7th ESENIAS Workshop with Scientific Conference
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences (IBER-BAS); East and South European Network for Invasive Alien Species (ESENIAS), Institute of Biodiversity and Ecosystem Research Bulgarian Academy of Sciences., 157.
https://hdl.handle.net/21.15107/rcub_rimsi_1727
Lenhardt M, Pekarik L, Skorić S, Smederevac-Lalić M, Hegediš A, Jaćimović M, Đikanović V. Influence of the diel period and different sampling methods on catch of gobiids at four locations in the inshore part of the Danube River. in 7th ESENIAS Workshop with Scientific Conference. 2017;:157.
https://hdl.handle.net/21.15107/rcub_rimsi_1727 .
Lenhardt, Mirjana, Pekarik, Ladislav, Skorić, Stefan, Smederevac-Lalić, Marija, Hegediš, Aleksandar, Jaćimović, Milica, Đikanović, Vesna, "Influence of the diel period and different sampling methods on catch of gobiids at four locations in the inshore part of the Danube River" in 7th ESENIAS Workshop with Scientific Conference (2017):157,
https://hdl.handle.net/21.15107/rcub_rimsi_1727 .

The influence of diel period on fish assemblage surveys by electro-fishing and beach seining at three locations in the inshore part of the Danube River

Lenhardt, Mirjana; Pekarik, Ladislav; Spasić, Slađana; Skorić, Stefan; Smederevac-Lalić, Marija; Hegediš, Aleksandar; Pucar, Milica; Đikanović, Vesna

(2016)

TY  - CONF
AU  - Lenhardt, Mirjana
AU  - Pekarik, Ladislav
AU  - Spasić, Slađana
AU  - Skorić, Stefan
AU  - Smederevac-Lalić, Marija
AU  - Hegediš, Aleksandar
AU  - Pucar, Milica
AU  - Đikanović, Vesna
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2400
C3  - The 41st International Association for Danube Research (IAD) Conference, Sibiu, Romania
T1  - The influence of diel period on fish assemblage surveys by electro-fishing and beach seining at three locations in the inshore part of the Danube River
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2400
ER  - 
@conference{
author = "Lenhardt, Mirjana and Pekarik, Ladislav and Spasić, Slađana and Skorić, Stefan and Smederevac-Lalić, Marija and Hegediš, Aleksandar and Pucar, Milica and Đikanović, Vesna",
year = "2016",
journal = "The 41st International Association for Danube Research (IAD) Conference, Sibiu, Romania",
title = "The influence of diel period on fish assemblage surveys by electro-fishing and beach seining at three locations in the inshore part of the Danube River",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2400"
}
Lenhardt, M., Pekarik, L., Spasić, S., Skorić, S., Smederevac-Lalić, M., Hegediš, A., Pucar, M.,& Đikanović, V.. (2016). The influence of diel period on fish assemblage surveys by electro-fishing and beach seining at three locations in the inshore part of the Danube River. in The 41st International Association for Danube Research (IAD) Conference, Sibiu, Romania.
https://hdl.handle.net/21.15107/rcub_rimsi_2400
Lenhardt M, Pekarik L, Spasić S, Skorić S, Smederevac-Lalić M, Hegediš A, Pucar M, Đikanović V. The influence of diel period on fish assemblage surveys by electro-fishing and beach seining at three locations in the inshore part of the Danube River. in The 41st International Association for Danube Research (IAD) Conference, Sibiu, Romania. 2016;.
https://hdl.handle.net/21.15107/rcub_rimsi_2400 .
Lenhardt, Mirjana, Pekarik, Ladislav, Spasić, Slađana, Skorić, Stefan, Smederevac-Lalić, Marija, Hegediš, Aleksandar, Pucar, Milica, Đikanović, Vesna, "The influence of diel period on fish assemblage surveys by electro-fishing and beach seining at three locations in the inshore part of the Danube River" in The 41st International Association for Danube Research (IAD) Conference, Sibiu, Romania (2016),
https://hdl.handle.net/21.15107/rcub_rimsi_2400 .