TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1688
AB  - Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly 
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem 
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the 
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated 
municipal and industrial waste waters. 
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver 
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was 
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet 
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to 
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the 
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored 
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay 
on HepG2 cells showed significant difference between values of negative control samples and samples 
of water from effluent site and downstream of wastewater discharge. There was no significant 
difference between negative control and upstream samples of water. 
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem. 
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More 
importantly, because Serbia still doesn’t have waste water treatment plants, this kind of research 
points out the imperative for implementation of this facilities.
PB  - Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany
C3  - 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
T1  - Genotoxicity assessment of Danube River: in situ and in vitro methods.
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1688
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Kostić-Vuković, Jovana and Sunjog, Karolina and Višnjić Jeftić, Željka and Subotić, Srđan and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2021",
abstract = "Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly 
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem 
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the 
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated 
municipal and industrial waste waters. 
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver 
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was 
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet 
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to 
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the 
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored 
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay 
on HepG2 cells showed significant difference between values of negative control samples and samples 
of water from effluent site and downstream of wastewater discharge. There was no significant 
difference between negative control and upstream samples of water. 
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem. 
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More 
importantly, because Serbia still doesn’t have waste water treatment plants, this kind of research 
points out the imperative for implementation of this facilities.",
publisher = "Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany",
journal = "43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin",
title = "Genotoxicity assessment of Danube River: in situ and in vitro methods.",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1688"
}

Đorđević Aleksić, J., Kostić-Vuković, J., Sunjog, K., Višnjić Jeftić, Ž., Subotić, S., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2021). Genotoxicity assessment of Danube River: in situ and in vitro methods.. in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany..
https://hdl.handle.net/21.15107/rcub_rimsi_1688

Đorđević Aleksić J, Kostić-Vuković J, Sunjog K, Višnjić Jeftić Ž, Subotić S, Gačić Z, Lenhardt M, Vuković-Gačić B. Genotoxicity assessment of Danube River: in situ and in vitro methods.. in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin. 2021;.
https://hdl.handle.net/21.15107/rcub_rimsi_1688 .

Đorđević Aleksić, Jelena, Kostić-Vuković, Jovana, Sunjog, Karolina, Višnjić Jeftić, Željka, Subotić, Srđan, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Genotoxicity assessment of Danube River: in situ and in vitro methods." in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin (2021),
https://hdl.handle.net/21.15107/rcub_rimsi_1688 .