TY  - JOUR
AU  - Vasiljević, Zorka Z
AU  - Vunduk, Jovana
AU  - Bartolić, Dragana
AU  - Miskovic, Goran
AU  - Ognjanović, Miloš
AU  - Tadic, Nenad
AU  - Nikolić, Maria Vesna
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3209
AB  - Emission  of greenhouse gases and infectious diseases caused by improper agro-waste disposal has gained significant attention in recent years. To overcome these hurdles, agro-waste can be valorized into valuable bioactive compounds that act as reducing or stabilizing agents in the synthesis of nanomaterials. Herein, we report a simple circular approach using Citrus reticulata Blanco (C. reticulata) waste (peel powder/aqueous extract) as green reducing and capping/stabilizing agents and Zn nitrate/acetate precursors to synthesize ZnO nanoparticles (NPs) with efficient antimicrobial and photocatalytic activities. The obtained NPs crystallized in a hexagonal wurtzite structure and differed clearly in their morphology. UV-vis analysis of the nanoparticles showed a characteristic broad absorption band between 330 and 414 nm, belonging to ZnO NPs. Fourier transform infrared (FTIR) spectroscopy of ZnO NPs exhibited a Zn–O band close to 450 cm−1. The band gap values were in the range of 2.84-3.14 eV depending on the precursor and agent used. The crystallite size obtained from size-strain plots from measured XRD patterns was between 7 and 26 nm, with the strain between 16 and 4%.  The high crystalline nature of obtained ZnO NPs was confirmed by clear ring diffraction patterns and d-spacing values of the observed lattice fringes. ZnNPeelMan_400 and ZnNExtrMan have shown good stability as the Zeta potential was found to be around -20 mV and reduced particle aggregation. Photoluminescence analysis revealed different defects belonging to oxygen vacancies (VO+ and VO++) and zinc interstitial (Zni) sites. The presence of oxygen vacancies on the surface of ZnAcExtrMan_400 and ZnAcPeelMan_400 increased antimicrobial activity, specifically against gram-negative bacteria Escherichia coli (E.coli) and Salmonella enteritidis (S. enteritidis). ZnNextrMan with a minimal inhibitory concentration of 0.156 mg/ml was more effective against gram-positive bacteria, Staphylococcus aureus (S. aureus) revealing a high influence of particle size and shape on antimicrobial activity. In addition, the photocatalytic activity of the ZnO NPs was examined by assessing the degradation of acid green dye in an aqueous solution under UV light irradiation. ZnAcPeelMan_400 exhibited excellent photocatalytic activity (94%) within 90 minutes after irradiation compared to other obtained ZnO NPs.
PB  - American Chemical Society
T2  - ACS Applied Bio Materials
T1  - An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity
EP  - 3032
SP  - 3014
VL  - 7
DO  - 10.1021/acsabm.4c00079
ER  - 

@article{
author = "Vasiljević, Zorka Z and Vunduk, Jovana and Bartolić, Dragana and Miskovic, Goran and Ognjanović, Miloš and Tadic, Nenad and Nikolić, Maria Vesna",
year = "2024",
abstract = "Emission  of greenhouse gases and infectious diseases caused by improper agro-waste disposal has gained significant attention in recent years. To overcome these hurdles, agro-waste can be valorized into valuable bioactive compounds that act as reducing or stabilizing agents in the synthesis of nanomaterials. Herein, we report a simple circular approach using Citrus reticulata Blanco (C. reticulata) waste (peel powder/aqueous extract) as green reducing and capping/stabilizing agents and Zn nitrate/acetate precursors to synthesize ZnO nanoparticles (NPs) with efficient antimicrobial and photocatalytic activities. The obtained NPs crystallized in a hexagonal wurtzite structure and differed clearly in their morphology. UV-vis analysis of the nanoparticles showed a characteristic broad absorption band between 330 and 414 nm, belonging to ZnO NPs. Fourier transform infrared (FTIR) spectroscopy of ZnO NPs exhibited a Zn–O band close to 450 cm−1. The band gap values were in the range of 2.84-3.14 eV depending on the precursor and agent used. The crystallite size obtained from size-strain plots from measured XRD patterns was between 7 and 26 nm, with the strain between 16 and 4%.  The high crystalline nature of obtained ZnO NPs was confirmed by clear ring diffraction patterns and d-spacing values of the observed lattice fringes. ZnNPeelMan_400 and ZnNExtrMan have shown good stability as the Zeta potential was found to be around -20 mV and reduced particle aggregation. Photoluminescence analysis revealed different defects belonging to oxygen vacancies (VO+ and VO++) and zinc interstitial (Zni) sites. The presence of oxygen vacancies on the surface of ZnAcExtrMan_400 and ZnAcPeelMan_400 increased antimicrobial activity, specifically against gram-negative bacteria Escherichia coli (E.coli) and Salmonella enteritidis (S. enteritidis). ZnNextrMan with a minimal inhibitory concentration of 0.156 mg/ml was more effective against gram-positive bacteria, Staphylococcus aureus (S. aureus) revealing a high influence of particle size and shape on antimicrobial activity. In addition, the photocatalytic activity of the ZnO NPs was examined by assessing the degradation of acid green dye in an aqueous solution under UV light irradiation. ZnAcPeelMan_400 exhibited excellent photocatalytic activity (94%) within 90 minutes after irradiation compared to other obtained ZnO NPs.",
publisher = "American Chemical Society",
journal = "ACS Applied Bio Materials",
title = "An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity",
pages = "3032-3014",
volume = "7",
doi = "10.1021/acsabm.4c00079"
}

Vasiljević, Z. Z., Vunduk, J., Bartolić, D., Miskovic, G., Ognjanović, M., Tadic, N.,& Nikolić, M. V.. (2024). An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity. in ACS Applied Bio Materials
American Chemical Society., 7, 3014-3032.
https://doi.org/10.1021/acsabm.4c00079

Vasiljević ZZ, Vunduk J, Bartolić D, Miskovic G, Ognjanović M, Tadic N, Nikolić MV. An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity. in ACS Applied Bio Materials. 2024;7:3014-3032.
doi:10.1021/acsabm.4c00079 .

Vasiljević, Zorka Z, Vunduk, Jovana, Bartolić, Dragana, Miskovic, Goran, Ognjanović, Miloš, Tadic, Nenad, Nikolić, Maria Vesna, "An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity" in ACS Applied Bio Materials, 7 (2024):3014-3032,
https://doi.org/10.1021/acsabm.4c00079 . .

TY  - CONF
AU  - Vasiljević, Zorka Z
AU  - Vunduk, Jovana
AU  - Dojčinović, Milena
AU  - Bartolić, Dragana
AU  - Ognjanović, Miloš
AU  - Tadic, Nenad
AU  - Mišković, Goran
AU  - Nikolić, Maria Vesna
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2132
AB  - Green synthesis is a more sustainable option using renewable biomass such as plants as
reducing or stabilizing agents compared to toxic chemical compounds. These biological
substances also behave as capping agents, which control the size and shape of the
nanoparticles. In this work, ZnO nanoparticles (NPs) have been prepared via a simple, low
cost and ecofriendly method using citrus fruit peel and extracts as biological reducing agents.
Zinc nitrate and zinc acetate were used as a source of zinc ions.XRD analysis revealed the
formation of a ZnO wurtzite phase without impurities. Synthesized ZnO NPs with an average
electronic band gap ∼3 eV were obtained and found to have round-like, hexagonal-like or
needle-like structures depending on precursor type. EDS analysis showed a homogeoneous
distribution in Zn and O elements, attributed to single-phase ZnO constituents. Antibacterial
and antioxidant activities of synthesized NPs were evaluated. Obtained results showed that
ZnO synthesized from nitrate precursors are more effective in inhibiting growth of
Salmonella and Staphylococcus Aureus. Antioxidant activity of ZnO NPs determined using
CUPRAC and ABTS assays showed higher activity of ZnO obtained using nitrate precursors.
The maximum scavenging activity of 90% was observed at the concentration of 10 mg/ml
PB  - Serbian Ceramic Society
C3  - Serbian Ceramic Society Conference - ADVANCED CERAMICS AND APPLICATION XI Program and the Book of Abstracts
T1  - Biosynthesis of ZnO nanoparticles using agro-waste with antibacterial and antioxidant activity
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2132
ER  - 

@conference{
author = "Vasiljević, Zorka Z and Vunduk, Jovana and Dojčinović, Milena and Bartolić, Dragana and Ognjanović, Miloš and Tadic, Nenad and Mišković, Goran and Nikolić, Maria Vesna",
year = "2023",
abstract = "Green synthesis is a more sustainable option using renewable biomass such as plants as
reducing or stabilizing agents compared to toxic chemical compounds. These biological
substances also behave as capping agents, which control the size and shape of the
nanoparticles. In this work, ZnO nanoparticles (NPs) have been prepared via a simple, low
cost and ecofriendly method using citrus fruit peel and extracts as biological reducing agents.
Zinc nitrate and zinc acetate were used as a source of zinc ions.XRD analysis revealed the
formation of a ZnO wurtzite phase without impurities. Synthesized ZnO NPs with an average
electronic band gap ∼3 eV were obtained and found to have round-like, hexagonal-like or
needle-like structures depending on precursor type. EDS analysis showed a homogeoneous
distribution in Zn and O elements, attributed to single-phase ZnO constituents. Antibacterial
and antioxidant activities of synthesized NPs were evaluated. Obtained results showed that
ZnO synthesized from nitrate precursors are more effective in inhibiting growth of
Salmonella and Staphylococcus Aureus. Antioxidant activity of ZnO NPs determined using
CUPRAC and ABTS assays showed higher activity of ZnO obtained using nitrate precursors.
The maximum scavenging activity of 90% was observed at the concentration of 10 mg/ml",
publisher = "Serbian Ceramic Society",
journal = "Serbian Ceramic Society Conference - ADVANCED CERAMICS AND APPLICATION XI Program and the Book of Abstracts",
title = "Biosynthesis of ZnO nanoparticles using agro-waste with antibacterial and antioxidant activity",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2132"
}

Vasiljević, Z. Z., Vunduk, J., Dojčinović, M., Bartolić, D., Ognjanović, M., Tadic, N., Mišković, G.,& Nikolić, M. V.. (2023). Biosynthesis of ZnO nanoparticles using agro-waste with antibacterial and antioxidant activity. in Serbian Ceramic Society Conference - ADVANCED CERAMICS AND APPLICATION XI Program and the Book of Abstracts
Serbian Ceramic Society., 43.
https://hdl.handle.net/21.15107/rcub_rimsi_2132

Vasiljević ZZ, Vunduk J, Dojčinović M, Bartolić D, Ognjanović M, Tadic N, Mišković G, Nikolić MV. Biosynthesis of ZnO nanoparticles using agro-waste with antibacterial and antioxidant activity. in Serbian Ceramic Society Conference - ADVANCED CERAMICS AND APPLICATION XI Program and the Book of Abstracts. 2023;:43.
https://hdl.handle.net/21.15107/rcub_rimsi_2132 .

Vasiljević, Zorka Z, Vunduk, Jovana, Dojčinović, Milena, Bartolić, Dragana, Ognjanović, Miloš, Tadic, Nenad, Mišković, Goran, Nikolić, Maria Vesna, "Biosynthesis of ZnO nanoparticles using agro-waste with antibacterial and antioxidant activity" in Serbian Ceramic Society Conference - ADVANCED CERAMICS AND APPLICATION XI Program and the Book of Abstracts (2023):43,
https://hdl.handle.net/21.15107/rcub_rimsi_2132 .