TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Gačić, Zoran
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kracun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1014
AB  - The impact of etoposide (ETO), vincristine sulfate (VIN), and cisplatin (CP) on the DNA damage level was studied in vivo and in vitro on hemocytes of freshwater mussels Unio sp. (Unio pictorum/Unio tumidus) using the alkaline comet assay. For in vivo experiments, the mussels were exposed in static system for 72 h. For in vitro experiments, treatment was performed in primary cultures of hemocytes for 22 h. The DNA damage level was analyzed by Comet Assay IV software. Increase of damage was detected for ETO (40 and 100 mu M) in vivo and in vitro and for VIN (0.04 and 0.1 mu M) in vivo. CP did not induce an increase of DNA damage, but post-treatment with hydrogen peroxide indicated existence of DNA crosslinks in specimens treated with 4 mu M CP. Effective concentrations of selected cytostatics are at least one (CP) or several orders of magnitude (ETO, VIN) higher comparing to the concentrations in hospital wastewater.
PB  - Wiley, Hoboken
T2  - Clean-Soil Air Water
T1  - Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus
EP  - 1476
IS  - 11
SP  - 1471
VL  - 44
DO  - 10.1002/clen.201500482
ER  - 

@article{
author = "Kolarević, Stoimir and Gačić, Zoran and Kostić-Vuković, Jovana and Sunjog, Karolina and Kracun-Kolarević, Margareta and Paunović, Momir and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2016",
abstract = "The impact of etoposide (ETO), vincristine sulfate (VIN), and cisplatin (CP) on the DNA damage level was studied in vivo and in vitro on hemocytes of freshwater mussels Unio sp. (Unio pictorum/Unio tumidus) using the alkaline comet assay. For in vivo experiments, the mussels were exposed in static system for 72 h. For in vitro experiments, treatment was performed in primary cultures of hemocytes for 22 h. The DNA damage level was analyzed by Comet Assay IV software. Increase of damage was detected for ETO (40 and 100 mu M) in vivo and in vitro and for VIN (0.04 and 0.1 mu M) in vivo. CP did not induce an increase of DNA damage, but post-treatment with hydrogen peroxide indicated existence of DNA crosslinks in specimens treated with 4 mu M CP. Effective concentrations of selected cytostatics are at least one (CP) or several orders of magnitude (ETO, VIN) higher comparing to the concentrations in hospital wastewater.",
publisher = "Wiley, Hoboken",
journal = "Clean-Soil Air Water",
title = "Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus",
pages = "1476-1471",
number = "11",
volume = "44",
doi = "10.1002/clen.201500482"
}

Kolarević, S., Gačić, Z., Kostić-Vuković, J., Sunjog, K., Kracun-Kolarević, M., Paunović, M., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2016). Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus. in Clean-Soil Air Water
Wiley, Hoboken., 44(11), 1471-1476.
https://doi.org/10.1002/clen.201500482

Kolarević S, Gačić Z, Kostić-Vuković J, Sunjog K, Kracun-Kolarević M, Paunović M, Knezevic-Vukcević J, Vukovic-Gacic B. Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus. in Clean-Soil Air Water. 2016;44(11):1471-1476.
doi:10.1002/clen.201500482 .

Kolarević, Stoimir, Gačić, Zoran, Kostić-Vuković, Jovana, Sunjog, Karolina, Kracun-Kolarević, Margareta, Paunović, Momir, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus" in Clean-Soil Air Water, 44, no. 11 (2016):1471-1476,
https://doi.org/10.1002/clen.201500482 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Milovanović, D.
AU  - Avdović, M.
AU  - Oalde, Mariana
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Nikolić, B.
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/973
AB  - In this study we investigated the influence of preparation of the bacterial inoculum for a microdilution susceptibility test, e.g., the effect of its optical density, on assessment of the minimum inhibitory concentrations (MIC). The approach employed in the majority of microdilution susceptibility studies is use of the same optical density for preparation of inoculums for different bacterial strains. In the present work, this approach was questioned by determining the ratio between the optical density and the number of bacteria in cultures. We also investigated whether the number of bacteria in inoculums can affect assessment of the MIC value for two antibiotics of broad spectra, rifampicin and streptomycin. The study was performed on four Gram-positive and four Gram-negative bacteria (ATCC collection) commonly used to investigate antimicrobial potential. The ratio between the optical density and number of bacteria in cultures was determined for each strain, and a strong linear correlation was detected. However, it was evident that different bacteria have different cell numbers at the same OD600 value. Based on the obtained results, inoculums for selected strains were prepared to obtain final cell numbers of 103, 104, 105 and 106 /well in the microdilution assay. Two different approaches were used in determining the MIC for rifampicin and streptomycin: approximation of MIC with IC90 and the resazurin reduction assay. Our results indicated that the ratio between optical density and cell numbers is not constant and use of the same OD for inoculums for all strains can therefore lead to misinterpretation of the MIC values. We also observed influence of cell numbers in inoculums in determination of MIC values. For both approaches used (approximation of MIC with IC90 and the resazurin reduction assay), the same trend was detected: antibiotics had the highest potency in experiments with the lowest bacteria cell number (103/well). The lowest cell number (103/well) is not recommended, as it can lead to false susceptibility results and to partial reduction of resazurin, which further complicates MIC determination. A final cell number of 104/well can therefore be recommended as optimal.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Optimisation of the microdilution method for detection of minimum inhibitory concentration values in selected bacteria
EP  - 36
IS  - 1
SP  - 29
VL  - 40
DO  - 10.5281/zenodo.48751
ER  - 

@article{
author = "Kolarević, Stoimir and Milovanović, D. and Avdović, M. and Oalde, Mariana and Kostić-Vuković, Jovana and Sunjog, Karolina and Nikolić, B. and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we investigated the influence of preparation of the bacterial inoculum for a microdilution susceptibility test, e.g., the effect of its optical density, on assessment of the minimum inhibitory concentrations (MIC). The approach employed in the majority of microdilution susceptibility studies is use of the same optical density for preparation of inoculums for different bacterial strains. In the present work, this approach was questioned by determining the ratio between the optical density and the number of bacteria in cultures. We also investigated whether the number of bacteria in inoculums can affect assessment of the MIC value for two antibiotics of broad spectra, rifampicin and streptomycin. The study was performed on four Gram-positive and four Gram-negative bacteria (ATCC collection) commonly used to investigate antimicrobial potential. The ratio between the optical density and number of bacteria in cultures was determined for each strain, and a strong linear correlation was detected. However, it was evident that different bacteria have different cell numbers at the same OD600 value. Based on the obtained results, inoculums for selected strains were prepared to obtain final cell numbers of 103, 104, 105 and 106 /well in the microdilution assay. Two different approaches were used in determining the MIC for rifampicin and streptomycin: approximation of MIC with IC90 and the resazurin reduction assay. Our results indicated that the ratio between optical density and cell numbers is not constant and use of the same OD for inoculums for all strains can therefore lead to misinterpretation of the MIC values. We also observed influence of cell numbers in inoculums in determination of MIC values. For both approaches used (approximation of MIC with IC90 and the resazurin reduction assay), the same trend was detected: antibiotics had the highest potency in experiments with the lowest bacteria cell number (103/well). The lowest cell number (103/well) is not recommended, as it can lead to false susceptibility results and to partial reduction of resazurin, which further complicates MIC determination. A final cell number of 104/well can therefore be recommended as optimal.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Optimisation of the microdilution method for detection of minimum inhibitory concentration values in selected bacteria",
pages = "36-29",
number = "1",
volume = "40",
doi = "10.5281/zenodo.48751"
}

Kolarević, S., Milovanović, D., Avdović, M., Oalde, M., Kostić-Vuković, J., Sunjog, K., Nikolić, B., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2016). Optimisation of the microdilution method for detection of minimum inhibitory concentration values in selected bacteria. in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 40(1), 29-36.
https://doi.org/10.5281/zenodo.48751

Kolarević S, Milovanović D, Avdović M, Oalde M, Kostić-Vuković J, Sunjog K, Nikolić B, Knezevic-Vukcević J, Vukovic-Gacic B. Optimisation of the microdilution method for detection of minimum inhibitory concentration values in selected bacteria. in Botanica Serbica. 2016;40(1):29-36.
doi:10.5281/zenodo.48751 .

Kolarević, Stoimir, Milovanović, D., Avdović, M., Oalde, Mariana, Kostić-Vuković, Jovana, Sunjog, Karolina, Nikolić, B., Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "Optimisation of the microdilution method for detection of minimum inhibitory concentration values in selected bacteria" in Botanica Serbica, 40, no. 1 (2016):29-36,
https://doi.org/10.5281/zenodo.48751 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Slobodnik, Jaroslav
AU  - Liska, Igor
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/970
AB  - In this study we assessed the level of genotoxic pollution along the Danube River by measuring the level of DNA damage in the haemocytes of freshwater mussels of Unio sp. (Unio pictorum/Unio tumidus) and Sinanodonta woodiana. The comet assay was used for the assessment of DNA damage. The research was performed on 34 out of 68 sites analysed within the Joint Danube Survey 3 the world's biggest river research expedition of its kind in 2013. During research, 2285 river kilometres were covered with an average distance of 68 km between the sites. The complex data set on concentrations of various substances present in water, suspended particulate matter and sediment on investigated sites gave the opportunity to identify the groups of xenobiotics which mostly affect the studied biomarker DNA damage. The highest levels of DNA damage were recorded in the section VI (Panonnian Plain), which is under the impact of untreated wastewater discharges. Both positive and negative influences of the large tributaries on the level of genotoxicity in the Danube River were evident. Significant correlation in response was detected between the studied species of freshwater mussels. The level of DNA damage in mussels correlated with concentrations of compounds from the group of hazardous priority substances (polycyclic aromatic hydrocarbons), persistent organic pollutants (dioxins) and emerging pollutants (Oxazepam, Chloridazon-desphenyl).
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3
EP  - 385
SP  - 377
VL  - 540
DO  - 10.1016/j.scitotenv.2015.06.061
ER  - 

@article{
author = "Kolarević, Stoimir and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Slobodnik, Jaroslav and Liska, Igor and Gačić, Zoran and Paunović, Momir and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we assessed the level of genotoxic pollution along the Danube River by measuring the level of DNA damage in the haemocytes of freshwater mussels of Unio sp. (Unio pictorum/Unio tumidus) and Sinanodonta woodiana. The comet assay was used for the assessment of DNA damage. The research was performed on 34 out of 68 sites analysed within the Joint Danube Survey 3 the world's biggest river research expedition of its kind in 2013. During research, 2285 river kilometres were covered with an average distance of 68 km between the sites. The complex data set on concentrations of various substances present in water, suspended particulate matter and sediment on investigated sites gave the opportunity to identify the groups of xenobiotics which mostly affect the studied biomarker DNA damage. The highest levels of DNA damage were recorded in the section VI (Panonnian Plain), which is under the impact of untreated wastewater discharges. Both positive and negative influences of the large tributaries on the level of genotoxicity in the Danube River were evident. Significant correlation in response was detected between the studied species of freshwater mussels. The level of DNA damage in mussels correlated with concentrations of compounds from the group of hazardous priority substances (polycyclic aromatic hydrocarbons), persistent organic pollutants (dioxins) and emerging pollutants (Oxazepam, Chloridazon-desphenyl).",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3",
pages = "385-377",
volume = "540",
doi = "10.1016/j.scitotenv.2015.06.061"
}

Kolarević, S., Kracun-Kolarević, M., Kostić-Vuković, J., Slobodnik, J., Liska, I., Gačić, Z., Paunović, M., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2016). Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3. in Science of the Total Environment
Elsevier, Amsterdam., 540, 377-385.
https://doi.org/10.1016/j.scitotenv.2015.06.061

Kolarević S, Kracun-Kolarević M, Kostić-Vuković J, Slobodnik J, Liska I, Gačić Z, Paunović M, Knezevic-Vukcević J, Vukovic-Gacic B. Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3. in Science of the Total Environment. 2016;540:377-385.
doi:10.1016/j.scitotenv.2015.06.061 .

Kolarević, Stoimir, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Slobodnik, Jaroslav, Liska, Igor, Gačić, Zoran, Paunović, Momir, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3" in Science of the Total Environment, 540 (2016):377-385,
https://doi.org/10.1016/j.scitotenv.2015.06.061 . .

TY  - JOUR
AU  - Aborgiba, Mustafa
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Elbahi, Samia
AU  - Knezevic-Vukcević, Jelena
AU  - Lenhardt, Mirjana
AU  - Paunović, Momir
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/936
AB  - Extreme hydrological events, such as water scarcity and flooding, can modify the effect of other stressors present in aquatic environment, which could result in the significant changes in the ecosystem functioning. Presence and interaction of various stressors (genotoxic pollutants) in the environment can influence the integrity of DNA molecules in aquatic organisms which can be negatively reflected on the individual, population and community levels. Therefore, in this study we have investigated the impact of flooding, in terms of genotoxicity, on organisms belonging to different trophic levels. The study was carried out on the site situated in the lower stretch of the Sava River which faced devastating effects of severe flooding in May 2014. The flooding occurred during our field experiment and this event provided a unique opportunity to assess its influence to the environment. The in situ effects of this specific situation were monitored by measuring physical, chemical and microbiological parameters of water, and by comparing the level of DNA damage in coelomocytes and haemocytes of freshwater worms Branchiura sowerbyi, haemocytes of freshwater mussels Unto tumidus and blood cells of freshwater fish Abramis bjoerkna/Abramis sapa, by means of the comet assay. Our study indicated that the flooding had a significant impact on water quality by decreasing the amount and discharge rate of urban wastewaters but simultaneously introducing contaminants from the nearby fly ash disposal field into river by runoff, which had diverse effects on the level of DNA damage in the studied organisms. This indicates that the assessment of genotoxic pollution in situ is strongly affected by the choice of the bioindicator organism.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River
EP  - 367
SP  - 358
VL  - 540
DO  - 10.1016/j.scitotenv.2015.03.120
ER  - 

@article{
author = "Aborgiba, Mustafa and Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Elbahi, Samia and Knezevic-Vukcević, Jelena and Lenhardt, Mirjana and Paunović, Momir and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2016",
abstract = "Extreme hydrological events, such as water scarcity and flooding, can modify the effect of other stressors present in aquatic environment, which could result in the significant changes in the ecosystem functioning. Presence and interaction of various stressors (genotoxic pollutants) in the environment can influence the integrity of DNA molecules in aquatic organisms which can be negatively reflected on the individual, population and community levels. Therefore, in this study we have investigated the impact of flooding, in terms of genotoxicity, on organisms belonging to different trophic levels. The study was carried out on the site situated in the lower stretch of the Sava River which faced devastating effects of severe flooding in May 2014. The flooding occurred during our field experiment and this event provided a unique opportunity to assess its influence to the environment. The in situ effects of this specific situation were monitored by measuring physical, chemical and microbiological parameters of water, and by comparing the level of DNA damage in coelomocytes and haemocytes of freshwater worms Branchiura sowerbyi, haemocytes of freshwater mussels Unto tumidus and blood cells of freshwater fish Abramis bjoerkna/Abramis sapa, by means of the comet assay. Our study indicated that the flooding had a significant impact on water quality by decreasing the amount and discharge rate of urban wastewaters but simultaneously introducing contaminants from the nearby fly ash disposal field into river by runoff, which had diverse effects on the level of DNA damage in the studied organisms. This indicates that the assessment of genotoxic pollution in situ is strongly affected by the choice of the bioindicator organism.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River",
pages = "367-358",
volume = "540",
doi = "10.1016/j.scitotenv.2015.03.120"
}

Aborgiba, M., Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Elbahi, S., Knezevic-Vukcević, J., Lenhardt, M., Paunović, M., Gačić, Z.,& Vukovic-Gacic, B.. (2016). Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River. in Science of the Total Environment
Elsevier, Amsterdam., 540, 358-367.
https://doi.org/10.1016/j.scitotenv.2015.03.120

Aborgiba M, Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Elbahi S, Knezevic-Vukcević J, Lenhardt M, Paunović M, Gačić Z, Vukovic-Gacic B. Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River. in Science of the Total Environment. 2016;540:358-367.
doi:10.1016/j.scitotenv.2015.03.120 .

Aborgiba, Mustafa, Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Elbahi, Samia, Knezevic-Vukcević, Jelena, Lenhardt, Mirjana, Paunović, Momir, Gačić, Zoran, Vukovic-Gacic, Branka, "Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River" in Science of the Total Environment, 540 (2016):358-367,
https://doi.org/10.1016/j.scitotenv.2015.03.120 . .

TY  - CHAP
AU  - Kapetanović, D.
AU  - Vardić, Smrzlić, I.
AU  - Valić, D.
AU  - Teskeredžić, E.
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Tomović, Jelena M
AU  - Kracun-Kolarević, Margareta
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/929
AB  - Data on the microbiological quality of the freshwater systems under the anthropogenic influence, such as the Sava River, are of the major importance for the water resource management. Furthermore, analyses of the microbial quality of fish meat provide information of the fish as a valuable food resource from the investigated river basin. The health status of the fish, including dynamics of infection and biodiversity of endoparasites, is important bioindicator of changes in the ecosystem structure and function. For the ecosystem-based approach to the Sava River management, investigations of microbiological quality of the Sava River water and the meat of the European chub as the bioindicator organism, as well as dynamics of infection/biodiversity of intestinal parasites Acanthocephala, were performed. The survey comprised the data collected in periods 2005, 2006 and 2012. Microbiological investigation of water was performed in 2006 and 2012, while microbiological analyses of fish meat and ichthyo-parasitological investigation took place during 2005–2006. A high number of heterotrophic bacteria were recorded during 2006 survey, confirmed by the distinctly higher values of the three faecal indicators (total coliform, E. coli and enterococci), and indicated poor water quality downstream of the cities Zagreb and Velika Gorica, as a result of the municipal sewage outlets. The results from 2012 survey indicated the existence of moderate to critical faecal and organic pollution in all samples. Accumulation of the bacteria in the European chub meat was mainly uniform along the watercourse within standards and limitations for the human consumption. Sampling sites downstream cities of Zagreb and Velika Gorica were characterised with the lower prevalence and abundance of two common species of the chub intestinal acanthocephalan parasites, Pomphorhynchus laevis and Acanthocephalus anguillae. Poor microbiological quality of the water and lower distribution of chub intestinal parasites were related to the anthropogenic influence, downstream of the urban areas.
PB  - Springer Verlag
T2  - Handbook of Environmental Chemistry
T1  - Microbial Characterisation of the Sava River
EP  - 228
SP  - 201
VL  - 31
DO  - 10.1007/978-3-662-44034-6_9
ER  - 

@inbook{
author = "Kapetanović, D. and Vardić, Smrzlić, I. and Valić, D. and Teskeredžić, E. and Kolarević, Stoimir and Sunjog, Karolina and Tomović, Jelena M and Kracun-Kolarević, Margareta and Knezevic-Vukcević, Jelena and Paunović, Momir and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2015",
abstract = "Data on the microbiological quality of the freshwater systems under the anthropogenic influence, such as the Sava River, are of the major importance for the water resource management. Furthermore, analyses of the microbial quality of fish meat provide information of the fish as a valuable food resource from the investigated river basin. The health status of the fish, including dynamics of infection and biodiversity of endoparasites, is important bioindicator of changes in the ecosystem structure and function. For the ecosystem-based approach to the Sava River management, investigations of microbiological quality of the Sava River water and the meat of the European chub as the bioindicator organism, as well as dynamics of infection/biodiversity of intestinal parasites Acanthocephala, were performed. The survey comprised the data collected in periods 2005, 2006 and 2012. Microbiological investigation of water was performed in 2006 and 2012, while microbiological analyses of fish meat and ichthyo-parasitological investigation took place during 2005–2006. A high number of heterotrophic bacteria were recorded during 2006 survey, confirmed by the distinctly higher values of the three faecal indicators (total coliform, E. coli and enterococci), and indicated poor water quality downstream of the cities Zagreb and Velika Gorica, as a result of the municipal sewage outlets. The results from 2012 survey indicated the existence of moderate to critical faecal and organic pollution in all samples. Accumulation of the bacteria in the European chub meat was mainly uniform along the watercourse within standards and limitations for the human consumption. Sampling sites downstream cities of Zagreb and Velika Gorica were characterised with the lower prevalence and abundance of two common species of the chub intestinal acanthocephalan parasites, Pomphorhynchus laevis and Acanthocephalus anguillae. Poor microbiological quality of the water and lower distribution of chub intestinal parasites were related to the anthropogenic influence, downstream of the urban areas.",
publisher = "Springer Verlag",
journal = "Handbook of Environmental Chemistry",
booktitle = "Microbial Characterisation of the Sava River",
pages = "228-201",
volume = "31",
doi = "10.1007/978-3-662-44034-6_9"
}

Kapetanović, D., Vardić, S. I., Valić, D., Teskeredžić, E., Kolarević, S., Sunjog, K., Tomović, J. M., Kracun-Kolarević, M., Knezevic-Vukcević, J., Paunović, M., Gačić, Z.,& Vukovic-Gacic, B.. (2015). Microbial Characterisation of the Sava River. in Handbook of Environmental Chemistry
Springer Verlag., 31, 201-228.
https://doi.org/10.1007/978-3-662-44034-6_9

Kapetanović D, Vardić SI, Valić D, Teskeredžić E, Kolarević S, Sunjog K, Tomović JM, Kracun-Kolarević M, Knezevic-Vukcević J, Paunović M, Gačić Z, Vukovic-Gacic B. Microbial Characterisation of the Sava River. in Handbook of Environmental Chemistry. 2015;31:201-228.
doi:10.1007/978-3-662-44034-6_9 .

Kapetanović, D., Vardić, Smrzlić, I., Valić, D., Teskeredžić, E., Kolarević, Stoimir, Sunjog, Karolina, Tomović, Jelena M, Kracun-Kolarević, Margareta, Knezevic-Vukcević, Jelena, Paunović, Momir, Gačić, Zoran, Vukovic-Gacic, Branka, "Microbial Characterisation of the Sava River" in Handbook of Environmental Chemistry, 31 (2015):201-228,
https://doi.org/10.1007/978-3-662-44034-6_9 . .

TY  - CHAP
AU  - Vukovic-Gacic, Branka
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Tomović, Jelena M
AU  - Kracun-Kolarević, Margareta
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Gačić, Zoran
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/928
AB  - Genotoxicity monitoring of the lower stretch of the Sava River was performed by the combined approach of in situ assessment of genotoxicity and active biomonitoring of two species of mussels from the Unionidae family, Unio pictorum and Unio tumidus. Genotoxic response was studied using comet assay on hemocytes. For active biomonitoring, the mussels were acclimated to controlled laboratory conditions for 10 days and then exposed at two sites in the Sava River in the area of the city of Belgrade. Hemolymph of exposed specimens of each species was taken after 7, 14, and 30 days of exposure. For in situ assessment, the mussels were collected from five sites in the lower flow of the Sava River. The mussels were sampled immediately after the acclimation served as controls in both types of monitoring procedures. The results of our studies indicated the presence of genotoxic pollution at all studied sites at the Sava River. The level of DNA damage varied at different sites depending on the source and level of pollution. The response to genotoxic pollution was evident at the site in the urban area of Belgrade city, as well as at the sites far from the large urban settlements, suggesting that the lower flow of the Sava River is under pollution pressure.
PB  - Springer Verlag
T2  - Handbook of Environmental Chemistry
T1  - Genotoxicological Studies of Lower Stretch of the Sava River
EP  - 452
SP  - 437
VL  - 31
DO  - 10.1007/978-3-662-44034-6_16
ER  - 

@inbook{
author = "Vukovic-Gacic, Branka and Kolarević, Stoimir and Sunjog, Karolina and Tomović, Jelena M and Kracun-Kolarević, Margareta and Knezevic-Vukcević, Jelena and Paunović, Momir and Gačić, Zoran",
year = "2015",
abstract = "Genotoxicity monitoring of the lower stretch of the Sava River was performed by the combined approach of in situ assessment of genotoxicity and active biomonitoring of two species of mussels from the Unionidae family, Unio pictorum and Unio tumidus. Genotoxic response was studied using comet assay on hemocytes. For active biomonitoring, the mussels were acclimated to controlled laboratory conditions for 10 days and then exposed at two sites in the Sava River in the area of the city of Belgrade. Hemolymph of exposed specimens of each species was taken after 7, 14, and 30 days of exposure. For in situ assessment, the mussels were collected from five sites in the lower flow of the Sava River. The mussels were sampled immediately after the acclimation served as controls in both types of monitoring procedures. The results of our studies indicated the presence of genotoxic pollution at all studied sites at the Sava River. The level of DNA damage varied at different sites depending on the source and level of pollution. The response to genotoxic pollution was evident at the site in the urban area of Belgrade city, as well as at the sites far from the large urban settlements, suggesting that the lower flow of the Sava River is under pollution pressure.",
publisher = "Springer Verlag",
journal = "Handbook of Environmental Chemistry",
booktitle = "Genotoxicological Studies of Lower Stretch of the Sava River",
pages = "452-437",
volume = "31",
doi = "10.1007/978-3-662-44034-6_16"
}

Vukovic-Gacic, B., Kolarević, S., Sunjog, K., Tomović, J. M., Kracun-Kolarević, M., Knezevic-Vukcević, J., Paunović, M.,& Gačić, Z.. (2015). Genotoxicological Studies of Lower Stretch of the Sava River. in Handbook of Environmental Chemistry
Springer Verlag., 31, 437-452.
https://doi.org/10.1007/978-3-662-44034-6_16

Vukovic-Gacic B, Kolarević S, Sunjog K, Tomović JM, Kracun-Kolarević M, Knezevic-Vukcević J, Paunović M, Gačić Z. Genotoxicological Studies of Lower Stretch of the Sava River. in Handbook of Environmental Chemistry. 2015;31:437-452.
doi:10.1007/978-3-662-44034-6_16 .

Vukovic-Gacic, Branka, Kolarević, Stoimir, Sunjog, Karolina, Tomović, Jelena M, Kracun-Kolarević, Margareta, Knezevic-Vukcević, Jelena, Paunović, Momir, Gačić, Zoran, "Genotoxicological Studies of Lower Stretch of the Sava River" in Handbook of Environmental Chemistry, 31 (2015):437-452,
https://doi.org/10.1007/978-3-662-44034-6_16 . .

TY  - JOUR
AU  - Gačić, Zoran
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kracun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/822
AB  - The impact of in vivo and in vitro exposure to anticancer drug 5-Fluorouracil (5-FU) on the level of DNA damage was evaluated using comet assay on haemocytes of freshwater mussels Unio pictorum and Unio tumidus. For the in vivo experiment, the groups of 5 mussels per concentration were exposed for 72 h to 5-FU (0.04, 0.4, 4, 40 and 100 mu M) with 0.4 mu M being the lowest concentration to induce significant DNA damage. For the in vitro experiment, the primary cultures of haemocytes were treated with 0.04, 0.4, 4 and 40 mu M 5-FU for 22 h and the treatment with CdCl2 was used as a positive control. In contrast to in vivo exposure, 5-FU did not induce significant increase of DNA damage in vitro, possibly because of the absence of haemocytes proliferation in primary cultures.
PB  - Elsevier Sci Ltd, Oxford
T2  - Environmental Pollution
T1  - The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus
EP  - 150
SP  - 145
VL  - 191
DO  - 10.1016/j.envpol.2014.04.024
ER  - 

@article{
author = "Gačić, Zoran and Kolarević, Stoimir and Sunjog, Karolina and Kracun-Kolarević, Margareta and Paunović, Momir and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2014",
abstract = "The impact of in vivo and in vitro exposure to anticancer drug 5-Fluorouracil (5-FU) on the level of DNA damage was evaluated using comet assay on haemocytes of freshwater mussels Unio pictorum and Unio tumidus. For the in vivo experiment, the groups of 5 mussels per concentration were exposed for 72 h to 5-FU (0.04, 0.4, 4, 40 and 100 mu M) with 0.4 mu M being the lowest concentration to induce significant DNA damage. For the in vitro experiment, the primary cultures of haemocytes were treated with 0.04, 0.4, 4 and 40 mu M 5-FU for 22 h and the treatment with CdCl2 was used as a positive control. In contrast to in vivo exposure, 5-FU did not induce significant increase of DNA damage in vitro, possibly because of the absence of haemocytes proliferation in primary cultures.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Environmental Pollution",
title = "The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus",
pages = "150-145",
volume = "191",
doi = "10.1016/j.envpol.2014.04.024"
}

Gačić, Z., Kolarević, S., Sunjog, K., Kracun-Kolarević, M., Paunović, M., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2014). The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus. in Environmental Pollution
Elsevier Sci Ltd, Oxford., 191, 145-150.
https://doi.org/10.1016/j.envpol.2014.04.024

Gačić Z, Kolarević S, Sunjog K, Kracun-Kolarević M, Paunović M, Knezevic-Vukcević J, Vukovic-Gacic B. The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus. in Environmental Pollution. 2014;191:145-150.
doi:10.1016/j.envpol.2014.04.024 .

Gačić, Zoran, Kolarević, Stoimir, Sunjog, Karolina, Kracun-Kolarević, Margareta, Paunović, Momir, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus" in Environmental Pollution, 191 (2014):145-150,
https://doi.org/10.1016/j.envpol.2014.04.024 . .

TY  - JOUR
AU  - Vukovic-Gacic, Branka
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Tomović, Jelena M
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Gačić, Zoran
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/820
AB  - Genotoxic response of freshwater mussels U. tumidus and U. pictorum to environmental stress was studied using comet assay on hemocytes and gill cells. The mussels were acclimated to controlled laboratory conditions for 10 days, and then exposed at 4 sites in the Sava and Danube rivers in the area of the city of Belgrade. Samples of each species were taken after 7, 14, and 30 days of exposure. The mussels sampled immediately after acclimation served as controls. Genotoxic response in both species was induced earlier at sites receiving untreated wastewaters from the city's main collectors (7 days), than at the site receiving only domestic wastewaters from small settlements located upstream from the city (30 days). There was a correlation between the comet tail intensity values in tissues of exposed mussels and the concentrations of zinc, copper, iron, and arsenic at the exposure sites. The genotoxic responses in both tissues of U. pictorum and in hemocytes of U. tumidus were similar, while U. tumidus gill cells failed to exhibit significant genotoxic response at two sites. These findings, together with higher mortality of U. tumidus at the most polluted sites, promote U. pictorum as a model for genotoxicity monitoring in freshwater environments.
PB  - Springer, Dordrecht
T2  - Hydrobiologia
T1  - Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress
EP  - 231
IS  - 1
SP  - 221
VL  - 735
DO  - 10.1007/s10750-013-1513-x
ER  - 

@article{
author = "Vukovic-Gacic, Branka and Kolarević, Stoimir and Sunjog, Karolina and Tomović, Jelena M and Knezevic-Vukcević, Jelena and Paunović, Momir and Gačić, Zoran",
year = "2014",
abstract = "Genotoxic response of freshwater mussels U. tumidus and U. pictorum to environmental stress was studied using comet assay on hemocytes and gill cells. The mussels were acclimated to controlled laboratory conditions for 10 days, and then exposed at 4 sites in the Sava and Danube rivers in the area of the city of Belgrade. Samples of each species were taken after 7, 14, and 30 days of exposure. The mussels sampled immediately after acclimation served as controls. Genotoxic response in both species was induced earlier at sites receiving untreated wastewaters from the city's main collectors (7 days), than at the site receiving only domestic wastewaters from small settlements located upstream from the city (30 days). There was a correlation between the comet tail intensity values in tissues of exposed mussels and the concentrations of zinc, copper, iron, and arsenic at the exposure sites. The genotoxic responses in both tissues of U. pictorum and in hemocytes of U. tumidus were similar, while U. tumidus gill cells failed to exhibit significant genotoxic response at two sites. These findings, together with higher mortality of U. tumidus at the most polluted sites, promote U. pictorum as a model for genotoxicity monitoring in freshwater environments.",
publisher = "Springer, Dordrecht",
journal = "Hydrobiologia",
title = "Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress",
pages = "231-221",
number = "1",
volume = "735",
doi = "10.1007/s10750-013-1513-x"
}

Vukovic-Gacic, B., Kolarević, S., Sunjog, K., Tomović, J. M., Knezevic-Vukcević, J., Paunović, M.,& Gačić, Z.. (2014). Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress. in Hydrobiologia
Springer, Dordrecht., 735(1), 221-231.
https://doi.org/10.1007/s10750-013-1513-x

Vukovic-Gacic B, Kolarević S, Sunjog K, Tomović JM, Knezevic-Vukcević J, Paunović M, Gačić Z. Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress. in Hydrobiologia. 2014;735(1):221-231.
doi:10.1007/s10750-013-1513-x .

Vukovic-Gacic, Branka, Kolarević, Stoimir, Sunjog, Karolina, Tomović, Jelena M, Knezevic-Vukcević, Jelena, Paunović, Momir, Gačić, Zoran, "Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress" in Hydrobiologia, 735, no. 1 (2014):221-231,
https://doi.org/10.1007/s10750-013-1513-x . .

TY  - JOUR
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Heberger, K
AU  - Gačić, Zoran
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
AU  - Lenhardt, Mirjana
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/730
AB  - The genotoxic potential of waters in six rivers and reservoirs from Serbia was monitored in different tissues of chub (Squalius cephalus L. 1758) with the alkaline comet assay. The comet assay, or single-cell gel electrophoresis, has a wide application as a simple and sensitive method for evaluating DNA damage in fish exposed to various xenobiotics in the aquatic environment. Three types of cells, erythrocytes, gill cells, and liver cells, were used for assessing DNA damage. Images of randomly selected cells were analyzed with a Leica fluorescence microscope and image analysis by software (Comet Assay IV Image analysis system, PI, UK). Three parameters (tail length-l, tail intensity-i, and Olive tail moment-m) were analyzed on 1,700 nuclei per cell type. The procedure for sum of ranking differences (SRD) was implemented to compare different types of cells and different parameters for estimation of DNA damage. Regarding our nine different estimations of genotoxicity: tail length, intensity, and moment in erythrocytes (rel, rei, rem), liver cells (rll, rli, rlm), and gill cells (rgl, rgi, rgm), the SRD procedure has shown that the Olive tail moment and tail intensity are (almost) equally good parameters; the SRD value was lower for the tail moment and tail intensity than for tail length in the case of all types of cells. The least reliable parameter was rel; close to the borderline case were rei, rll, and rgl (similar to 5 % probability of random ranking).
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Comparison of comet assay parameters for estimation of genotoxicity by sum of ranking differences
EP  - 4885
IS  - 14
SP  - 4879
VL  - 405
DO  - 10.1007/s00216-013-6909-y
ER  - 

@article{
author = "Sunjog, Karolina and Kolarević, Stoimir and Heberger, K and Gačić, Zoran and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka and Lenhardt, Mirjana",
year = "2013",
abstract = "The genotoxic potential of waters in six rivers and reservoirs from Serbia was monitored in different tissues of chub (Squalius cephalus L. 1758) with the alkaline comet assay. The comet assay, or single-cell gel electrophoresis, has a wide application as a simple and sensitive method for evaluating DNA damage in fish exposed to various xenobiotics in the aquatic environment. Three types of cells, erythrocytes, gill cells, and liver cells, were used for assessing DNA damage. Images of randomly selected cells were analyzed with a Leica fluorescence microscope and image analysis by software (Comet Assay IV Image analysis system, PI, UK). Three parameters (tail length-l, tail intensity-i, and Olive tail moment-m) were analyzed on 1,700 nuclei per cell type. The procedure for sum of ranking differences (SRD) was implemented to compare different types of cells and different parameters for estimation of DNA damage. Regarding our nine different estimations of genotoxicity: tail length, intensity, and moment in erythrocytes (rel, rei, rem), liver cells (rll, rli, rlm), and gill cells (rgl, rgi, rgm), the SRD procedure has shown that the Olive tail moment and tail intensity are (almost) equally good parameters; the SRD value was lower for the tail moment and tail intensity than for tail length in the case of all types of cells. The least reliable parameter was rel; close to the borderline case were rei, rll, and rgl (similar to 5 % probability of random ranking).",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Comparison of comet assay parameters for estimation of genotoxicity by sum of ranking differences",
pages = "4885-4879",
number = "14",
volume = "405",
doi = "10.1007/s00216-013-6909-y"
}

Sunjog, K., Kolarević, S., Heberger, K., Gačić, Z., Knezevic-Vukcević, J., Vukovic-Gacic, B.,& Lenhardt, M.. (2013). Comparison of comet assay parameters for estimation of genotoxicity by sum of ranking differences. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 405(14), 4879-4885.
https://doi.org/10.1007/s00216-013-6909-y

Sunjog K, Kolarević S, Heberger K, Gačić Z, Knezevic-Vukcević J, Vukovic-Gacic B, Lenhardt M. Comparison of comet assay parameters for estimation of genotoxicity by sum of ranking differences. in Analytical and Bioanalytical Chemistry. 2013;405(14):4879-4885.
doi:10.1007/s00216-013-6909-y .

Sunjog, Karolina, Kolarević, Stoimir, Heberger, K, Gačić, Zoran, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, Lenhardt, Mirjana, "Comparison of comet assay parameters for estimation of genotoxicity by sum of ranking differences" in Analytical and Bioanalytical Chemistry, 405, no. 14 (2013):4879-4885,
https://doi.org/10.1007/s00216-013-6909-y . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Kracun, Margareta
AU  - Vasiljević, Bozica
AU  - Tomović, Jelena M
AU  - Vukovic-Gacic, Branka
AU  - Gačić, Zoran
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/703
AB  - This study was undertaken to investigate the potential of the freshwater mussel Sinanodonta woodiana for detection of genotoxic pollution of the environment. Study was performed at two sites in the Velika Morava River, from May 2010 to February 2011. The alkaline comet assay on haemocytes was used, and the olive tail moment (OTM) was chosen as a measure of DNA damage. The specimens held on acclimation under controlled laboratory conditions for 10 d were used as a control. Chemical analysis revealed the presence of phosphates and increased concentrations of zinc, copper and nickel at both sites during the entire sampling period. The values of OTM in mussels collected from the environment, significantly correlated with the concentration of zinc (r = 0.6248), temperature (r = 0.7006) and dissolved oxygen (r = 0.7738). Seasonal variations in genotoxic response were observed, with the highest OTM values obtained during summer months. Preliminary results of the in vitro study indicated the effect of water temperature on genotoxic response to zinc and cadmium in S. woodiana suggesting that the presence of genotoxic pollutants during months with lower temperature could be under-estimated. Obtained results indicate that S. woodiana could be a valuable tool for active biomonitoring of aquatic environments and emphasizes the importance of seasonal genotoxic monitoring with this organism.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay
EP  - 251
IS  - 2
SP  - 243
VL  - 93
DO  - 10.1016/j.chemosphere.2013.04.073
ER  - 

@article{
author = "Kolarević, Stoimir and Knezevic-Vukcević, Jelena and Paunović, Momir and Kracun, Margareta and Vasiljević, Bozica and Tomović, Jelena M and Vukovic-Gacic, Branka and Gačić, Zoran",
year = "2013",
abstract = "This study was undertaken to investigate the potential of the freshwater mussel Sinanodonta woodiana for detection of genotoxic pollution of the environment. Study was performed at two sites in the Velika Morava River, from May 2010 to February 2011. The alkaline comet assay on haemocytes was used, and the olive tail moment (OTM) was chosen as a measure of DNA damage. The specimens held on acclimation under controlled laboratory conditions for 10 d were used as a control. Chemical analysis revealed the presence of phosphates and increased concentrations of zinc, copper and nickel at both sites during the entire sampling period. The values of OTM in mussels collected from the environment, significantly correlated with the concentration of zinc (r = 0.6248), temperature (r = 0.7006) and dissolved oxygen (r = 0.7738). Seasonal variations in genotoxic response were observed, with the highest OTM values obtained during summer months. Preliminary results of the in vitro study indicated the effect of water temperature on genotoxic response to zinc and cadmium in S. woodiana suggesting that the presence of genotoxic pollutants during months with lower temperature could be under-estimated. Obtained results indicate that S. woodiana could be a valuable tool for active biomonitoring of aquatic environments and emphasizes the importance of seasonal genotoxic monitoring with this organism.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay",
pages = "251-243",
number = "2",
volume = "93",
doi = "10.1016/j.chemosphere.2013.04.073"
}

Kolarević, S., Knezevic-Vukcević, J., Paunović, M., Kracun, M., Vasiljević, B., Tomović, J. M., Vukovic-Gacic, B.,& Gačić, Z.. (2013). Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay. in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 93(2), 243-251.
https://doi.org/10.1016/j.chemosphere.2013.04.073

Kolarević S, Knezevic-Vukcević J, Paunović M, Kracun M, Vasiljević B, Tomović JM, Vukovic-Gacic B, Gačić Z. Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay. in Chemosphere. 2013;93(2):243-251.
doi:10.1016/j.chemosphere.2013.04.073 .

Kolarević, Stoimir, Knezevic-Vukcević, Jelena, Paunović, Momir, Kracun, Margareta, Vasiljević, Bozica, Tomović, Jelena M, Vukovic-Gacic, Branka, Gačić, Zoran, "Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay" in Chemosphere, 93, no. 2 (2013):243-251,
https://doi.org/10.1016/j.chemosphere.2013.04.073 . .

TY  - JOUR
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Kolarević, Stoimir
AU  - Višnjić Jeftić, Željka
AU  - Jarić, Ivan
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
AU  - Lenhardt, Mirjana
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/617
AB  - The aim of this study was to analyze 16 trace elements (Al, As, B, Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Mo, Ni, Pb, Sr, and Zn) in different barbel (Barbus barbus) tissues and to detect the presence of genotoxic effects in erythrocytes with the alkaline comet assay. Barbel specimens were collected in the Danube river near Belgrade, Serbia, where the discharge of untreated communal and industrial wastewaters is likely to produce negative effects on fish residing in this area. The highest concentrations of Sr, Mn, Fe, Ba, B, and Al were found in gills, Mo and Cu in liver, and As and Zn in gonads. Concentrations of Zn and Fe were above maximum acceptable concentrations (MACs) in a number of gonad, gill, and liver samples. Three-year-old barbel specimens had higher tail moment and Zn concentrations in gills (1.71 and 51.20 mu g/g dw, resp.) than 5-year-old specimens (0.85 and 42.51 mu g/g dw, resp.). Results indicate that the younger barbel specimens might be more suitable for the monitoring of environmental pollution.
PB  - Hindawi Ltd, London
T2  - Scientific World Journal
T1  - Heavy Metal Accumulation and the Genotoxicity in Barbel (Barbus barbus) as Indicators of the Danube River Pollution
DO  - 10.1100/2012/351074
ER  - 

@article{
author = "Sunjog, Karolina and Gačić, Zoran and Kolarević, Stoimir and Višnjić Jeftić, Željka and Jarić, Ivan and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka and Lenhardt, Mirjana",
year = "2012",
abstract = "The aim of this study was to analyze 16 trace elements (Al, As, B, Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Mo, Ni, Pb, Sr, and Zn) in different barbel (Barbus barbus) tissues and to detect the presence of genotoxic effects in erythrocytes with the alkaline comet assay. Barbel specimens were collected in the Danube river near Belgrade, Serbia, where the discharge of untreated communal and industrial wastewaters is likely to produce negative effects on fish residing in this area. The highest concentrations of Sr, Mn, Fe, Ba, B, and Al were found in gills, Mo and Cu in liver, and As and Zn in gonads. Concentrations of Zn and Fe were above maximum acceptable concentrations (MACs) in a number of gonad, gill, and liver samples. Three-year-old barbel specimens had higher tail moment and Zn concentrations in gills (1.71 and 51.20 mu g/g dw, resp.) than 5-year-old specimens (0.85 and 42.51 mu g/g dw, resp.). Results indicate that the younger barbel specimens might be more suitable for the monitoring of environmental pollution.",
publisher = "Hindawi Ltd, London",
journal = "Scientific World Journal",
title = "Heavy Metal Accumulation and the Genotoxicity in Barbel (Barbus barbus) as Indicators of the Danube River Pollution",
doi = "10.1100/2012/351074"
}

Sunjog, K., Gačić, Z., Kolarević, S., Višnjić Jeftić, Ž., Jarić, I., Knezevic-Vukcević, J., Vukovic-Gacic, B.,& Lenhardt, M.. (2012). Heavy Metal Accumulation and the Genotoxicity in Barbel (Barbus barbus) as Indicators of the Danube River Pollution. in Scientific World Journal
Hindawi Ltd, London..
https://doi.org/10.1100/2012/351074

Sunjog K, Gačić Z, Kolarević S, Višnjić Jeftić Ž, Jarić I, Knezevic-Vukcević J, Vukovic-Gacic B, Lenhardt M. Heavy Metal Accumulation and the Genotoxicity in Barbel (Barbus barbus) as Indicators of the Danube River Pollution. in Scientific World Journal. 2012;.
doi:10.1100/2012/351074 .

Sunjog, Karolina, Gačić, Zoran, Kolarević, Stoimir, Višnjić Jeftić, Željka, Jarić, Ivan, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, Lenhardt, Mirjana, "Heavy Metal Accumulation and the Genotoxicity in Barbel (Barbus barbus) as Indicators of the Danube River Pollution" in Scientific World Journal (2012),
https://doi.org/10.1100/2012/351074 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Vasiljević, Bozica
AU  - Kracun, Margareta
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/526
AB  - In this study, we investigated the level of sanitary pollution and organic contamination of the Velika Morava River, the largest river in central Serbia. Samples of water for microbiological analysis were collected at 5 sites, monthly, from April 2010 to February 2011. Sanitary analysis, i.e. enumeration of total and fecal coliforms and intestinal enterococci, indicated moderate to critical fecal contamination, while organic load assessment (oligotroph to heterotroph ratio, index of phosphatase activity) revealed the category of moderately polluted water. We also investigated seasonal variations of these groups of bacteria and the factors that could contribute to these differences. Our results showed that the microbiological quality of the water in the Velika Morava River during different seasons is affected by numerous factors such as unequal loading of wastewaters, solar irradiation, and relations of flow/dilution and rainfall/runoff.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Seasonal variations of microbiological parameters of water quality of the Velika Morava river Serbia
EP  - 1027
IS  - 3
SP  - 1017
VL  - 64
DO  - 10.2298/ABS1203017K
ER  - 

@article{
author = "Kolarević, Stoimir and Knezevic-Vukcević, Jelena and Paunović, Momir and Vasiljević, Bozica and Kracun, Margareta and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2012",
abstract = "In this study, we investigated the level of sanitary pollution and organic contamination of the Velika Morava River, the largest river in central Serbia. Samples of water for microbiological analysis were collected at 5 sites, monthly, from April 2010 to February 2011. Sanitary analysis, i.e. enumeration of total and fecal coliforms and intestinal enterococci, indicated moderate to critical fecal contamination, while organic load assessment (oligotroph to heterotroph ratio, index of phosphatase activity) revealed the category of moderately polluted water. We also investigated seasonal variations of these groups of bacteria and the factors that could contribute to these differences. Our results showed that the microbiological quality of the water in the Velika Morava River during different seasons is affected by numerous factors such as unequal loading of wastewaters, solar irradiation, and relations of flow/dilution and rainfall/runoff.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Seasonal variations of microbiological parameters of water quality of the Velika Morava river Serbia",
pages = "1027-1017",
number = "3",
volume = "64",
doi = "10.2298/ABS1203017K"
}

Kolarević, S., Knezevic-Vukcević, J., Paunović, M., Vasiljević, B., Kracun, M., Gačić, Z.,& Vukovic-Gacic, B.. (2012). Seasonal variations of microbiological parameters of water quality of the Velika Morava river Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(3), 1017-1027.
https://doi.org/10.2298/ABS1203017K

Kolarević S, Knezevic-Vukcević J, Paunović M, Vasiljević B, Kracun M, Gačić Z, Vukovic-Gacic B. Seasonal variations of microbiological parameters of water quality of the Velika Morava river Serbia. in Archives of Biological Sciences. 2012;64(3):1017-1027.
doi:10.2298/ABS1203017K .

Kolarević, Stoimir, Knezevic-Vukcević, Jelena, Paunović, Momir, Vasiljević, Bozica, Kracun, Margareta, Gačić, Zoran, Vukovic-Gacic, Branka, "Seasonal variations of microbiological parameters of water quality of the Velika Morava river Serbia" in Archives of Biological Sciences, 64, no. 3 (2012):1017-1027,
https://doi.org/10.2298/ABS1203017K . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Tomović, Jelena M
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/483
AB  - The aim of this work was to examine the impact of urban wastewaters on the water quality of the Danube River in Serbia. Samples of water and sediments for microbiological analysis and genotoxicity monitoring were collected from 6 sites during spring and/or autumn 2010. Sanitary analysis, i.e. enumeration of total and fecal coliforms and intestinal enterococci, indicated moderate to critical fecal contamination, while organic load assessment (oligotroph to heterotroph ratio, index of phosphatase activity) revealed the category of moderately polluted water. Mercury-resistant bacteria were detected in all water samples, with high numbers at locations positioned downstream of Belgrade. There was no correlation of the microbiological parameters of the sediment and water samples. Genotoxicity monitoring, performed by the comet assay on hemocytes of mussels Sinanodonta woodiana, indicated a significant increase of DNA damage in mussels collected from the studied sites compared with the control group.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The anthropogenic impact on water quality of the river danube in Serbia: microbiological analysis and genotoxicity monitoring
EP  - 1217
IS  - 4
SP  - 1209
VL  - 63
DO  - 10.2298/ABS1104209K
ER  - 

@article{
author = "Kolarević, Stoimir and Knezevic-Vukcević, Jelena and Paunović, Momir and Tomović, Jelena M and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2011",
abstract = "The aim of this work was to examine the impact of urban wastewaters on the water quality of the Danube River in Serbia. Samples of water and sediments for microbiological analysis and genotoxicity monitoring were collected from 6 sites during spring and/or autumn 2010. Sanitary analysis, i.e. enumeration of total and fecal coliforms and intestinal enterococci, indicated moderate to critical fecal contamination, while organic load assessment (oligotroph to heterotroph ratio, index of phosphatase activity) revealed the category of moderately polluted water. Mercury-resistant bacteria were detected in all water samples, with high numbers at locations positioned downstream of Belgrade. There was no correlation of the microbiological parameters of the sediment and water samples. Genotoxicity monitoring, performed by the comet assay on hemocytes of mussels Sinanodonta woodiana, indicated a significant increase of DNA damage in mussels collected from the studied sites compared with the control group.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The anthropogenic impact on water quality of the river danube in Serbia: microbiological analysis and genotoxicity monitoring",
pages = "1217-1209",
number = "4",
volume = "63",
doi = "10.2298/ABS1104209K"
}

Kolarević, S., Knezevic-Vukcević, J., Paunović, M., Tomović, J. M., Gačić, Z.,& Vukovic-Gacic, B.. (2011). The anthropogenic impact on water quality of the river danube in Serbia: microbiological analysis and genotoxicity monitoring. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(4), 1209-1217.
https://doi.org/10.2298/ABS1104209K

Kolarević S, Knezevic-Vukcević J, Paunović M, Tomović JM, Gačić Z, Vukovic-Gacic B. The anthropogenic impact on water quality of the river danube in Serbia: microbiological analysis and genotoxicity monitoring. in Archives of Biological Sciences. 2011;63(4):1209-1217.
doi:10.2298/ABS1104209K .

Kolarević, Stoimir, Knezevic-Vukcević, Jelena, Paunović, Momir, Tomović, Jelena M, Gačić, Zoran, Vukovic-Gacic, Branka, "The anthropogenic impact on water quality of the river danube in Serbia: microbiological analysis and genotoxicity monitoring" in Archives of Biological Sciences, 63, no. 4 (2011):1209-1217,
https://doi.org/10.2298/ABS1104209K . .