TY  - CONF
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1959
AB  - In August 2021, symptoms of soft rot appeared on cabbage 
in Futog (Bačka, Vojvodina) locality known for traditional 
cabbage cultivation in Serbia. Symptoms appeared in the 
form of sunken and soft lesions on outer head leaves, while 
interior tissue was macerated, with cream to black 
discoloration. A strong, specific odor followed the 
breakdown of the affected tissue. From the 1 ha field, a 
total of 5 heads were collected for the isolation of causing 
pathogen. Margins of healthy and dead tissues were 
macerated and plated on Crystal Violet Pectate medium. 
Five creamy-white colonies forming pits in the medium 
were purified. These bacterial isolates were positive for 
pectinolytic activity on cabbage heads and potato slices. 
They were all facultative anaerobic, Gram-negative, 
catalase-positive, and oxidase-negative; unable to produce 
diffusible fluorescent pigments. Multilocus sequence 
typing of cabbage isolates was performed by sequencing 
of four housekeeping genes (proA, dnaX, icdA, mdh). 
Based on the nucleotide BLAST (National Center for 
Biotechnology Information, NCBI) analysis of the 
sequences of genes icdA and mdh, Serbian cabbage isolates 
were 100% identical with Pectobacterium carotovorum
subsp. odoriferum strain BC S7. Based on the dnaX gene, 
identity with the same strain was 99.79% and 99.57% 
based on the proA gene. Serbian cabbage isolates also 
showed 100% identity with other NCBI deposited P. 
odoriferum strains CFBP1878 and ATCC 25272 based on 
the icdA gene, as well as ICMP 11533 based on mdh gene. 
Therefore, this first report of P. odoriferum indicates a 
wider range of Pectobacterium spp. recently described in 
Serbia.
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference   on Plant Pathogenic Bacteria
T1  - First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia
SP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1959
ER  - 

@conference{
author = "Marković, Sanja and Popović, Tatjana and Mitrović, Petar and Iličić, Renata and Jelušić, Aleksandra",
year = "2022",
abstract = "In August 2021, symptoms of soft rot appeared on cabbage 
in Futog (Bačka, Vojvodina) locality known for traditional 
cabbage cultivation in Serbia. Symptoms appeared in the 
form of sunken and soft lesions on outer head leaves, while 
interior tissue was macerated, with cream to black 
discoloration. A strong, specific odor followed the 
breakdown of the affected tissue. From the 1 ha field, a 
total of 5 heads were collected for the isolation of causing 
pathogen. Margins of healthy and dead tissues were 
macerated and plated on Crystal Violet Pectate medium. 
Five creamy-white colonies forming pits in the medium 
were purified. These bacterial isolates were positive for 
pectinolytic activity on cabbage heads and potato slices. 
They were all facultative anaerobic, Gram-negative, 
catalase-positive, and oxidase-negative; unable to produce 
diffusible fluorescent pigments. Multilocus sequence 
typing of cabbage isolates was performed by sequencing 
of four housekeeping genes (proA, dnaX, icdA, mdh). 
Based on the nucleotide BLAST (National Center for 
Biotechnology Information, NCBI) analysis of the 
sequences of genes icdA and mdh, Serbian cabbage isolates 
were 100% identical with Pectobacterium carotovorum
subsp. odoriferum strain BC S7. Based on the dnaX gene, 
identity with the same strain was 99.79% and 99.57% 
based on the proA gene. Serbian cabbage isolates also 
showed 100% identity with other NCBI deposited P. 
odoriferum strains CFBP1878 and ATCC 25272 based on 
the icdA gene, as well as ICMP 11533 based on mdh gene. 
Therefore, this first report of P. odoriferum indicates a 
wider range of Pectobacterium spp. recently described in 
Serbia.",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference   on Plant Pathogenic Bacteria",
title = "First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia",
pages = "123",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1959"
}

Marković, S., Popović, T., Mitrović, P., Iličić, R.,& Jelušić, A.. (2022). First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini., 123.
https://hdl.handle.net/21.15107/rcub_rimsi_1959

Marković S, Popović T, Mitrović P, Iličić R, Jelušić A. First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria. 2022;:123.
https://hdl.handle.net/21.15107/rcub_rimsi_1959 .

Marković, Sanja, Popović, Tatjana, Mitrović, Petar, Iličić, Renata, Jelušić, Aleksandra, "First report of Pectobacterium odoriferum causing  bacterial soft rot of cabbage in Serbia" in 14th International Conference   on Plant Pathogenic Bacteria (2022):123,
https://hdl.handle.net/21.15107/rcub_rimsi_1959 .