TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kilsey
AU  - Oswald, Petar
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2044
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
EP  - 32
IS  - 1
SP  - 21
VL  - 38
DO  - 10.1093/mutage/geac024
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kilsey and Oswald, Petar and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
pages = "32-21",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024"
}

Jovanović Marić, J., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević Aleksić J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kilsey, Oswald, Petar, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Slobodnik, Jaroslav
AU  - Liska, Igor
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/970
AB  - In this study we assessed the level of genotoxic pollution along the Danube River by measuring the level of DNA damage in the haemocytes of freshwater mussels of Unio sp. (Unio pictorum/Unio tumidus) and Sinanodonta woodiana. The comet assay was used for the assessment of DNA damage. The research was performed on 34 out of 68 sites analysed within the Joint Danube Survey 3 the world's biggest river research expedition of its kind in 2013. During research, 2285 river kilometres were covered with an average distance of 68 km between the sites. The complex data set on concentrations of various substances present in water, suspended particulate matter and sediment on investigated sites gave the opportunity to identify the groups of xenobiotics which mostly affect the studied biomarker DNA damage. The highest levels of DNA damage were recorded in the section VI (Panonnian Plain), which is under the impact of untreated wastewater discharges. Both positive and negative influences of the large tributaries on the level of genotoxicity in the Danube River were evident. Significant correlation in response was detected between the studied species of freshwater mussels. The level of DNA damage in mussels correlated with concentrations of compounds from the group of hazardous priority substances (polycyclic aromatic hydrocarbons), persistent organic pollutants (dioxins) and emerging pollutants (Oxazepam, Chloridazon-desphenyl).
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3
EP  - 385
SP  - 377
VL  - 540
DO  - 10.1016/j.scitotenv.2015.06.061
ER  - 

@article{
author = "Kolarević, Stoimir and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Slobodnik, Jaroslav and Liska, Igor and Gačić, Zoran and Paunović, Momir and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we assessed the level of genotoxic pollution along the Danube River by measuring the level of DNA damage in the haemocytes of freshwater mussels of Unio sp. (Unio pictorum/Unio tumidus) and Sinanodonta woodiana. The comet assay was used for the assessment of DNA damage. The research was performed on 34 out of 68 sites analysed within the Joint Danube Survey 3 the world's biggest river research expedition of its kind in 2013. During research, 2285 river kilometres were covered with an average distance of 68 km between the sites. The complex data set on concentrations of various substances present in water, suspended particulate matter and sediment on investigated sites gave the opportunity to identify the groups of xenobiotics which mostly affect the studied biomarker DNA damage. The highest levels of DNA damage were recorded in the section VI (Panonnian Plain), which is under the impact of untreated wastewater discharges. Both positive and negative influences of the large tributaries on the level of genotoxicity in the Danube River were evident. Significant correlation in response was detected between the studied species of freshwater mussels. The level of DNA damage in mussels correlated with concentrations of compounds from the group of hazardous priority substances (polycyclic aromatic hydrocarbons), persistent organic pollutants (dioxins) and emerging pollutants (Oxazepam, Chloridazon-desphenyl).",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3",
pages = "385-377",
volume = "540",
doi = "10.1016/j.scitotenv.2015.06.061"
}

Kolarević, S., Kracun-Kolarević, M., Kostić-Vuković, J., Slobodnik, J., Liska, I., Gačić, Z., Paunović, M., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2016). Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3. in Science of the Total Environment
Elsevier, Amsterdam., 540, 377-385.
https://doi.org/10.1016/j.scitotenv.2015.06.061

Kolarević S, Kracun-Kolarević M, Kostić-Vuković J, Slobodnik J, Liska I, Gačić Z, Paunović M, Knezevic-Vukcević J, Vukovic-Gacic B. Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3. in Science of the Total Environment. 2016;540:377-385.
doi:10.1016/j.scitotenv.2015.06.061 .

Kolarević, Stoimir, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Slobodnik, Jaroslav, Liska, Igor, Gačić, Zoran, Paunović, Momir, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3" in Science of the Total Environment, 540 (2016):377-385,
https://doi.org/10.1016/j.scitotenv.2015.06.061 . .

TY  - JOUR
AU  - Deutschmann, Bjorn
AU  - Kolarević, Stoimir
AU  - Brack, Werner
AU  - Kaisarević, Sonja
AU  - Kostić-Vuković, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Liska, Igor
AU  - Paunović, Momir
AU  - Seiler, Thomas-Benjamin
AU  - Shao, Ying
AU  - Sipos, Sandor
AU  - Slobodnik, Jaroslav
AU  - Teodorović, Ivana
AU  - Vukovic-Gacic, Branka
AU  - Hollert, Henner
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/957
AB  - The Joint Danube Survey 3 (JDS3; the biggest river expedition in 2013) had offered the unique opportunity for a large-scale monitoring approach for biomarker response in feral fish collected along a Danube stretch from Kehlheim (DE) to Sulina (RO). The advantage of genotoxicity as a marker for pollution exposure in fish is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was in the focus of the biomarker investigations in fish during the expedition. Blood samples of common bleak (Alburnus alburnus) for the investigation of the micronucleus frequency and comet tail intensity of fragmented DNA material in erythrocytes were collected at 18 and 12 sampling sites, respectively. For 9 sampling sites same samples were used to compare the in-situ data for the comparable genotoxic endpoint in the micronucleus (MN) and comet assay (CM). The data of both.in-situ assays showed a significant correlation, indicating the strength and comparability of the data sets. Significant variation in DNA damage in-fish along the longitudinal profile of the Danube was demonstrated for both assays compared to reference sites. The results suggest that DNA damage in erythrocytes of fish was mainly affected by wastewater of highly populated regions. No linkage between the results and the general health/dietary status of the fish were revealed, whereas correlation with some genotoxicity drivers in the water phase, suspended particulate matter and sediments could be demonstrated.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay
EP  - 1449
SP  - 1441
VL  - 573
DO  - 10.1016/j.scitotenv.2016.07.175
ER  - 

@article{
author = "Deutschmann, Bjorn and Kolarević, Stoimir and Brack, Werner and Kaisarević, Sonja and Kostić-Vuković, Jovana and Kracun-Kolarević, Margareta and Liska, Igor and Paunović, Momir and Seiler, Thomas-Benjamin and Shao, Ying and Sipos, Sandor and Slobodnik, Jaroslav and Teodorović, Ivana and Vukovic-Gacic, Branka and Hollert, Henner",
year = "2016",
abstract = "The Joint Danube Survey 3 (JDS3; the biggest river expedition in 2013) had offered the unique opportunity for a large-scale monitoring approach for biomarker response in feral fish collected along a Danube stretch from Kehlheim (DE) to Sulina (RO). The advantage of genotoxicity as a marker for pollution exposure in fish is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was in the focus of the biomarker investigations in fish during the expedition. Blood samples of common bleak (Alburnus alburnus) for the investigation of the micronucleus frequency and comet tail intensity of fragmented DNA material in erythrocytes were collected at 18 and 12 sampling sites, respectively. For 9 sampling sites same samples were used to compare the in-situ data for the comparable genotoxic endpoint in the micronucleus (MN) and comet assay (CM). The data of both.in-situ assays showed a significant correlation, indicating the strength and comparability of the data sets. Significant variation in DNA damage in-fish along the longitudinal profile of the Danube was demonstrated for both assays compared to reference sites. The results suggest that DNA damage in erythrocytes of fish was mainly affected by wastewater of highly populated regions. No linkage between the results and the general health/dietary status of the fish were revealed, whereas correlation with some genotoxicity drivers in the water phase, suspended particulate matter and sediments could be demonstrated.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay",
pages = "1449-1441",
volume = "573",
doi = "10.1016/j.scitotenv.2016.07.175"
}

Deutschmann, B., Kolarević, S., Brack, W., Kaisarević, S., Kostić-Vuković, J., Kracun-Kolarević, M., Liska, I., Paunović, M., Seiler, T., Shao, Y., Sipos, S., Slobodnik, J., Teodorović, I., Vukovic-Gacic, B.,& Hollert, H.. (2016). Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay. in Science of the Total Environment
Elsevier, Amsterdam., 573, 1441-1449.
https://doi.org/10.1016/j.scitotenv.2016.07.175

Deutschmann B, Kolarević S, Brack W, Kaisarević S, Kostić-Vuković J, Kracun-Kolarević M, Liska I, Paunović M, Seiler T, Shao Y, Sipos S, Slobodnik J, Teodorović I, Vukovic-Gacic B, Hollert H. Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay. in Science of the Total Environment. 2016;573:1441-1449.
doi:10.1016/j.scitotenv.2016.07.175 .

Deutschmann, Bjorn, Kolarević, Stoimir, Brack, Werner, Kaisarević, Sonja, Kostić-Vuković, Jovana, Kracun-Kolarević, Margareta, Liska, Igor, Paunović, Momir, Seiler, Thomas-Benjamin, Shao, Ying, Sipos, Sandor, Slobodnik, Jaroslav, Teodorović, Ivana, Vukovic-Gacic, Branka, Hollert, Henner, "Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay" in Science of the Total Environment, 573 (2016):1441-1449,
https://doi.org/10.1016/j.scitotenv.2016.07.175 . .