TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Bijelic, Dunja
AU  - Milicevic, Katarina
AU  - Momcilovic, Miljana
AU  - Bogdanović Pristov, Jelena
AU  - Petkovic, Branka
AU  - Andjus, Pavle
AU  - Miljkovic, Djordje
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2006
AB  - Aims: Multiple sclerosis (MS) is an in ammatory autoimmune disorder of the central nervous system (CNS). Complex interactions between inltrating immune cells (IIC) and resident glial cells of the CNS cause myelin loss and neuronal dysfunction in MS. Here we aim to understand how naïve astrocytes functionally respond to the IIC invasion of the CNS. 
Methods: We measured calcium activity of naïve astrocytes in culture upon application of IIC. An experimental autoimmune encephalomyelitis (EAE) MS rat model was used to isolate IIC from the spinal cord of animals at the symptomatic stage. Naïve astrocytes were isolated from the spinal cord of WT rats. 
Results: We show that IIC and not the lymph node immune cells evoke vigorous increase in the astrocyte calcium activity. This IIC-induced calcium response depends on an autocrine activation of the purinergic P2X7 receptors on the naïve astrocytes.We also show that IIC induce ATP release from astrocytes by a mechanism that involves gap junctions and/or hemichannels activation and not the vesicular pathway. Our data indicate that ATP release and subsequent increase in the astrocytic calcium activity mainly depends on the cell-cell contact between naïve astrocytes and IIC. 
Conclusions: These results show that naïve astrocytes functionally respond to the IIC by augmented release of ATP. An increase in ATP release would alter astrocyte-neuron communication and a ect neuronal function in MS.
PB  - Serbian Neuroscience Society, Belgrade
C3  - Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract
T1  - ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY
SP  - 295
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2006
ER  - 

@conference{
author = "Nikolic, Ljiljana and Bijelic, Dunja and Milicevic, Katarina and Momcilovic, Miljana and Bogdanović Pristov, Jelena and Petkovic, Branka and Andjus, Pavle and Miljkovic, Djordje",
year = "2019",
abstract = "Aims: Multiple sclerosis (MS) is an in ammatory autoimmune disorder of the central nervous system (CNS). Complex interactions between inltrating immune cells (IIC) and resident glial cells of the CNS cause myelin loss and neuronal dysfunction in MS. Here we aim to understand how naïve astrocytes functionally respond to the IIC invasion of the CNS. 
Methods: We measured calcium activity of naïve astrocytes in culture upon application of IIC. An experimental autoimmune encephalomyelitis (EAE) MS rat model was used to isolate IIC from the spinal cord of animals at the symptomatic stage. Naïve astrocytes were isolated from the spinal cord of WT rats. 
Results: We show that IIC and not the lymph node immune cells evoke vigorous increase in the astrocyte calcium activity. This IIC-induced calcium response depends on an autocrine activation of the purinergic P2X7 receptors on the naïve astrocytes.We also show that IIC induce ATP release from astrocytes by a mechanism that involves gap junctions and/or hemichannels activation and not the vesicular pathway. Our data indicate that ATP release and subsequent increase in the astrocytic calcium activity mainly depends on the cell-cell contact between naïve astrocytes and IIC. 
Conclusions: These results show that naïve astrocytes functionally respond to the IIC by augmented release of ATP. An increase in ATP release would alter astrocyte-neuron communication and a ect neuronal function in MS.",
publisher = "Serbian Neuroscience Society, Belgrade",
journal = "Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract",
title = "ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY",
pages = "295",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2006"
}

Nikolic, L., Bijelic, D., Milicevic, K., Momcilovic, M., Bogdanović Pristov, J., Petkovic, B., Andjus, P.,& Miljkovic, D.. (2019). ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY. in Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract
Serbian Neuroscience Society, Belgrade., 295.
https://hdl.handle.net/21.15107/rcub_rimsi_2006

Nikolic L, Bijelic D, Milicevic K, Momcilovic M, Bogdanović Pristov J, Petkovic B, Andjus P, Miljkovic D. ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY. in Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract. 2019;:295.
https://hdl.handle.net/21.15107/rcub_rimsi_2006 .

Nikolic, Ljiljana, Bijelic, Dunja, Milicevic, Katarina, Momcilovic, Miljana, Bogdanović Pristov, Jelena, Petkovic, Branka, Andjus, Pavle, Miljkovic, Djordje, "ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY" in Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract (2019):295,
https://hdl.handle.net/21.15107/rcub_rimsi_2006 .