TY  - CONF
AU  - Lužajić, Tijana
AU  - Milošević, Ivan
AU  - Danilović Luković, Jelena
AU  - Marković, Danica
AU  - Milošević, Sandra
AU  - Kovačević Filipović, Milica
AU  - Radovanovic, Anita
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2734
AB  - Thyroid hormones (TH) play a pivotal role in the development of mammalian skin, and
are necessary for both the initiation and maintenance of hair growth. Adult hypothyroid rats
showed impaired epidermal proliferation, hair growth and wound healing. Stem cells (SCs)
located at the bulges of the hair follicles are responsible for hair cycling, and contribute to
the regeneration of the new epidermis after wounding. Therefore a reduction in the number or
function of bulge stem cells could be a cause of disturbed skin reepithelization and hair follicle
maintenance. We hypothesized that a decrease in TH during prenatal development would affect
the bulge stem cell population.
Subclinical hypothyroidism was induced with propylthiouracil through drinking water
in a dose of 1.5 mg/l in pregnant Albino Oxford rats, from the first day of gravidity and during
lactation. The control group was untreated. Five, seven-day-old pups were euthanized and
skin samples were taken from the dorsal part of their bodies. The number of hair follicles was
estimated on an area of 1mm2 of dermis. For the immunohistochemical study, proliferating cell
nuclear antigen (PCNA) (Santa Cruz Biotechnology) and NANOG (Thermo Scientific) antibodies
were used. The number of PCNA and NANOG positive cells was estimated on the area of 1mm2
of hair follicle.
The number of hair follicles was reduced and the expression of PCNA was decreased
in the cells of the inner and outer sheath of hair follicles in the hypothyroid pups compared to the
controls. They also had an increased number of NANOG positive bulge cells which we presume
are multipotent SCs.
Decreased expression of PCNA demonstrates low proliferation and increased
expression of NANOG, which may be explained in two ways: 1) the inhibited differentiation of
putative SCs and/or 2) the reduction of mobilization of these cells to the epidermis or sebaceous
gland. Our results confirm that the lack of TH influences the formation of hair follicles in early
infantile rat pups.
PB  - Faculty of Veterinary Medicine, University of Zagreb
C3  - 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb
T1  - Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population
EP  - 152
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2734
ER  - 

@conference{
author = "Lužajić, Tijana and Milošević, Ivan and Danilović Luković, Jelena and Marković, Danica and Milošević, Sandra and Kovačević Filipović, Milica and Radovanovic, Anita",
year = "2017",
abstract = "Thyroid hormones (TH) play a pivotal role in the development of mammalian skin, and
are necessary for both the initiation and maintenance of hair growth. Adult hypothyroid rats
showed impaired epidermal proliferation, hair growth and wound healing. Stem cells (SCs)
located at the bulges of the hair follicles are responsible for hair cycling, and contribute to
the regeneration of the new epidermis after wounding. Therefore a reduction in the number or
function of bulge stem cells could be a cause of disturbed skin reepithelization and hair follicle
maintenance. We hypothesized that a decrease in TH during prenatal development would affect
the bulge stem cell population.
Subclinical hypothyroidism was induced with propylthiouracil through drinking water
in a dose of 1.5 mg/l in pregnant Albino Oxford rats, from the first day of gravidity and during
lactation. The control group was untreated. Five, seven-day-old pups were euthanized and
skin samples were taken from the dorsal part of their bodies. The number of hair follicles was
estimated on an area of 1mm2 of dermis. For the immunohistochemical study, proliferating cell
nuclear antigen (PCNA) (Santa Cruz Biotechnology) and NANOG (Thermo Scientific) antibodies
were used. The number of PCNA and NANOG positive cells was estimated on the area of 1mm2
of hair follicle.
The number of hair follicles was reduced and the expression of PCNA was decreased
in the cells of the inner and outer sheath of hair follicles in the hypothyroid pups compared to the
controls. They also had an increased number of NANOG positive bulge cells which we presume
are multipotent SCs.
Decreased expression of PCNA demonstrates low proliferation and increased
expression of NANOG, which may be explained in two ways: 1) the inhibited differentiation of
putative SCs and/or 2) the reduction of mobilization of these cells to the epidermis or sebaceous
gland. Our results confirm that the lack of TH influences the formation of hair follicles in early
infantile rat pups.",
publisher = "Faculty of Veterinary Medicine, University of Zagreb",
journal = "7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb",
title = "Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population",
pages = "152-152",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2734"
}

Lužajić, T., Milošević, I., Danilović Luković, J., Marković, D., Milošević, S., Kovačević Filipović, M.,& Radovanovic, A.. (2017). Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb
Faculty of Veterinary Medicine, University of Zagreb., 152-152.
https://hdl.handle.net/21.15107/rcub_rimsi_2734

Lužajić T, Milošević I, Danilović Luković J, Marković D, Milošević S, Kovačević Filipović M, Radovanovic A. Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb. 2017;:152-152.
https://hdl.handle.net/21.15107/rcub_rimsi_2734 .

Lužajić, Tijana, Milošević, Ivan, Danilović Luković, Jelena, Marković, Danica, Milošević, Sandra, Kovačević Filipović, Milica, Radovanovic, Anita, "Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population" in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb (2017):152-152,
https://hdl.handle.net/21.15107/rcub_rimsi_2734 .

TY  - CONF
AU  - Milošević, Ivan
AU  - Danilović Luković, Jelena
AU  - Lužajić, Tijana
AU  - Milošević, Sandra
AU  - Radovanovic, Anita
AU  - Sourice-Petit, Sophie
AU  - Guicheux, Jerome
AU  - Kovačević Filipović, Milica
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2695
AB  - The effects of maternal hypothyroidism on the development and function of different
organs of their offspring are well known. It is unclear how its subclinical form affects some
of these processes, including endochondral ossification during prenatal and early postnatal
development. Our previous studies have demonstrated that hypothyroid rat pups had reduced
growth plate thickness.
The aim of the study was to assess bone turnover by defining the expression of tartrateresistant
acid phosphatase (TRAP), alkaline phosphatase tissue nonspecific isozyme (ALPL)
and mRNA for dentin matrix acidic phosphoprotein (DMP1) in the tibia of pups from subclinical
hypothyroid dams in early postnatal development. Subclinical hypothyroidism (SHT) was
induced with propylthiouracil through drinking water (1.5 mg/l) in pregnant Albino Oxford dams
from the first day of gravidity and during lactation. The control group was untreated. Five, sevenday-
old pups from each experimental group were euthanized. All histological examinations were
performed on paraffin sections of the proximal tibial growth plate. Histochemistry was used to
assess the expression of TRAP, immunohistochemistry for ALPL and in situ hybridization for
DMP1. The area of the TRAP positive signal, optical density of ALPL immunostaining, and the
number of DMP1 positive cells were evaluated using the Image J. Expression of TRAP and ALPL
in the proximal tibial growth plate were lower in the hypothyroid pups, and the number of cells
expressing mRNA for DMP1 was decreased.
TRAP is a marker of osteoclast activity. Osteoclasts are multinucleated cells which are
necessary for bone and mineralized cartilage resorption. In osteoblasts ALPL and DMP1 are
expressed and they have an important role in the process of endochondral ossification. As we
have shown that TRAP, ALPL and DMP1 are decreased, we may conclude that maternal SHT in
rats leads to compromised cartilage to bone transition, and delayed osteogenic differentiation
in the early infantile period.
PB  - Faculty of Veterinary Medicine, University of Zagreb, Croatia
C3  - 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017
T1  - Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns
EP  - 151
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2695
ER  - 

@conference{
author = "Milošević, Ivan and Danilović Luković, Jelena and Lužajić, Tijana and Milošević, Sandra and Radovanovic, Anita and Sourice-Petit, Sophie and Guicheux, Jerome and Kovačević Filipović, Milica",
year = "2017",
abstract = "The effects of maternal hypothyroidism on the development and function of different
organs of their offspring are well known. It is unclear how its subclinical form affects some
of these processes, including endochondral ossification during prenatal and early postnatal
development. Our previous studies have demonstrated that hypothyroid rat pups had reduced
growth plate thickness.
The aim of the study was to assess bone turnover by defining the expression of tartrateresistant
acid phosphatase (TRAP), alkaline phosphatase tissue nonspecific isozyme (ALPL)
and mRNA for dentin matrix acidic phosphoprotein (DMP1) in the tibia of pups from subclinical
hypothyroid dams in early postnatal development. Subclinical hypothyroidism (SHT) was
induced with propylthiouracil through drinking water (1.5 mg/l) in pregnant Albino Oxford dams
from the first day of gravidity and during lactation. The control group was untreated. Five, sevenday-
old pups from each experimental group were euthanized. All histological examinations were
performed on paraffin sections of the proximal tibial growth plate. Histochemistry was used to
assess the expression of TRAP, immunohistochemistry for ALPL and in situ hybridization for
DMP1. The area of the TRAP positive signal, optical density of ALPL immunostaining, and the
number of DMP1 positive cells were evaluated using the Image J. Expression of TRAP and ALPL
in the proximal tibial growth plate were lower in the hypothyroid pups, and the number of cells
expressing mRNA for DMP1 was decreased.
TRAP is a marker of osteoclast activity. Osteoclasts are multinucleated cells which are
necessary for bone and mineralized cartilage resorption. In osteoblasts ALPL and DMP1 are
expressed and they have an important role in the process of endochondral ossification. As we
have shown that TRAP, ALPL and DMP1 are decreased, we may conclude that maternal SHT in
rats leads to compromised cartilage to bone transition, and delayed osteogenic differentiation
in the early infantile period.",
publisher = "Faculty of Veterinary Medicine, University of Zagreb, Croatia",
journal = "7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017",
title = "Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns",
pages = "151-151",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2695"
}

Milošević, I., Danilović Luković, J., Lužajić, T., Milošević, S., Radovanovic, A., Sourice-Petit, S., Guicheux, J.,& Kovačević Filipović, M.. (2017). Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns. in 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017
Faculty of Veterinary Medicine, University of Zagreb, Croatia., 151-151.
https://hdl.handle.net/21.15107/rcub_rimsi_2695

Milošević I, Danilović Luković J, Lužajić T, Milošević S, Radovanovic A, Sourice-Petit S, Guicheux J, Kovačević Filipović M. Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns. in 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017. 2017;:151-151.
https://hdl.handle.net/21.15107/rcub_rimsi_2695 .

Milošević, Ivan, Danilović Luković, Jelena, Lužajić, Tijana, Milošević, Sandra, Radovanovic, Anita, Sourice-Petit, Sophie, Guicheux, Jerome, Kovačević Filipović, Milica, "Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns" in 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017 (2017):151-151,
https://hdl.handle.net/21.15107/rcub_rimsi_2695 .