TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kilsey
AU  - Oswald, Petar
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2044
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
EP  - 32
IS  - 1
SP  - 21
VL  - 38
DO  - 10.1093/mutage/geac024
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kilsey and Oswald, Petar and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
pages = "32-21",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024"
}

Jovanović Marić, J., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević Aleksić J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kilsey, Oswald, Petar, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Lozo, Jelena
AU  - Nikolić, Ivan
AU  - Berić, Tanja
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2040
AB  - Crna trulež kupusnjača, prouzrokovana fitopatogenom bakterijom Xanthomonas campestris pv. campestris (Xcc) jedna je od najvažnijih i destruktivnih bolesti koja u celom svetu, a poslednjih godina i kod nas izaziva velike ekonomske štete. Ova bakterija napada sve gajene predstavnike porodice Brassicaceae. Infekciju vrši putem lisnih hidatoda i ozleda i multiplicira se kroz vaskularno tkivo prouzrokujući lezije u obliku slova V. Cilj istraživanja ovog rada je ispitivanje genetičkog diverziteta 122 izolata Xcc, poreklom sa šest različitih domaćina (brokoli, kupus, karfiol, raštan, kelj i keleraba), koji su pokazivali simptome crne truleži. Uzorci sa obolelih biljaka su prikupljeni na nekoliko različitih lokaliteta na teritoriji Srbije u 2014. godini. Svi testirani izolati su preliminarno identifikovani kao Xcc na osnovu morfoloških i odgajivačkih odlika, patogenosti i molekularnih metoda PCR primenom specifičnog para prajmera XCF/XCR i sekvencioniranja gyrB gena. Genetička karakterizacija je obuhvatila primenu dve molekularne metode: elektroforezu u pulsirajućem polju (PFGE) i umnožavanje ponavljajućih elemenata (rep-PCR) korišćenjem prajmera komplementarnih određenim ponavljajućim ekstragenskim (REP-PCR), intergenskim (ERIC-PCR) i BOX elementima (BOX-PCR). ERIC- i REP-PCR fingerprinting tehnike razdvojile su sve Xcc izolate u četiri grupe, za razliku od BOX-PCR i PFGE tehnika, koji su dali iste paterne u slučaju svih testiranih izolata. Značajna korelacija između domaćina sa kog su izolati dobijeni kao i geografske distribucije poređenjem sa razlikama u dobijenim DNK fingerprinting paternima nije pronađena. Rezultati dobijeni u ovom radu pokazuju genetičku heterogenost Xcc populacije, što je u korelaciji sa prethodnim istraživanjima genetičkog diverziteta Xcc izolata, i iako verovarno klonalna u Srbiji, na šta ukazuju rezultati PFGE analize, dodatna analiza je neophodna.
AB  - Black rot caused by phytopathogenic bacteria Xanthomonas campestris pv. campestris (Xcc) is one of the most important and destructive bacterial diseases which causes enormous economic damages on crucifer crops worldwide, and lately in our country as well. This serious and destructive vascular disease is present in all cultivated Brassicaceae crops. The infection mainly expends through the leaf hydathodes and injuries, systematically moving through the vascular tissue, which all leads to the formation of necrotic V-shaped lesions. The aim of this study was to gain insight into the genetic diversity of one hundred and twenty two Xcc isolates obtained from six different crucifers (broccoli, cabbage, cauliflower, collard greens, kale and kohlrabi) with black rot symptoms. Samples were collected from different regions in Serbia in 2014. All tested isolates were preliminary identified as Xcc based on morphological and cultural characteristics, pathogenicity, PCR with specific primer set XCF/XCR and gyrB gene sequencing. Study of genetic diversity included two molecular typing methods: pulsed-field gel electrophoresis (PFGE) and repetitive sequence-based PCR (rep-PCR) of repetitive extragenic palindromic (REP-PCR), enterobacterial repetitive intergenic consensus (ERIC-PCR) and BOX elements (BOX-PCR). ERIC- and REP-PCR fingerprinting techniques divided all Xcc isolates into four groups, unlike BOX-PCR and PFGE which yielded same banding patterns for each of the tested isolates. Based on the obtained banding patterns, significant correlation between hosts from which the isolates were obtained, as well as between their geographic distributions was not observed. The results obtained in this work indicate that Xcc population is genetically diverse, which is in correlation with previous findings of genetic diversity within Xcc strains, although it is probably clonal in Serbia, according to PFGE, but additional analysis is required.
PB  - UDRUŽENJE MIKROBIOLOGA SRBIJE, Nemanjina 6, Beograd
C3  - 12th Congress of Serbian microbiologists with intenational participation “MICROMED 2018 REGIO”, Belgrade, Serbia
T1  - Genetic characterization of Xanthomonas campestris pv. campestris isolates from different hosts using PFGE and Rep-pcr techniques
T1  - Genetička karakterizacija Xanthomonas campestris pv. campestris poreklom sa različitih domaćina upotrebom PFGE i rep-pcr metoda
EP  - 204
SP  - 203
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2040
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Jelušić, Aleksandra and Mitrović, Petar and Stanković, Slaviša and Lozo, Jelena and Nikolić, Ivan and Berić, Tanja",
year = "2018",
abstract = "Crna trulež kupusnjača, prouzrokovana fitopatogenom bakterijom Xanthomonas campestris pv. campestris (Xcc) jedna je od najvažnijih i destruktivnih bolesti koja u celom svetu, a poslednjih godina i kod nas izaziva velike ekonomske štete. Ova bakterija napada sve gajene predstavnike porodice Brassicaceae. Infekciju vrši putem lisnih hidatoda i ozleda i multiplicira se kroz vaskularno tkivo prouzrokujući lezije u obliku slova V. Cilj istraživanja ovog rada je ispitivanje genetičkog diverziteta 122 izolata Xcc, poreklom sa šest različitih domaćina (brokoli, kupus, karfiol, raštan, kelj i keleraba), koji su pokazivali simptome crne truleži. Uzorci sa obolelih biljaka su prikupljeni na nekoliko različitih lokaliteta na teritoriji Srbije u 2014. godini. Svi testirani izolati su preliminarno identifikovani kao Xcc na osnovu morfoloških i odgajivačkih odlika, patogenosti i molekularnih metoda PCR primenom specifičnog para prajmera XCF/XCR i sekvencioniranja gyrB gena. Genetička karakterizacija je obuhvatila primenu dve molekularne metode: elektroforezu u pulsirajućem polju (PFGE) i umnožavanje ponavljajućih elemenata (rep-PCR) korišćenjem prajmera komplementarnih određenim ponavljajućim ekstragenskim (REP-PCR), intergenskim (ERIC-PCR) i BOX elementima (BOX-PCR). ERIC- i REP-PCR fingerprinting tehnike razdvojile su sve Xcc izolate u četiri grupe, za razliku od BOX-PCR i PFGE tehnika, koji su dali iste paterne u slučaju svih testiranih izolata. Značajna korelacija između domaćina sa kog su izolati dobijeni kao i geografske distribucije poređenjem sa razlikama u dobijenim DNK fingerprinting paternima nije pronađena. Rezultati dobijeni u ovom radu pokazuju genetičku heterogenost Xcc populacije, što je u korelaciji sa prethodnim istraživanjima genetičkog diverziteta Xcc izolata, i iako verovarno klonalna u Srbiji, na šta ukazuju rezultati PFGE analize, dodatna analiza je neophodna., Black rot caused by phytopathogenic bacteria Xanthomonas campestris pv. campestris (Xcc) is one of the most important and destructive bacterial diseases which causes enormous economic damages on crucifer crops worldwide, and lately in our country as well. This serious and destructive vascular disease is present in all cultivated Brassicaceae crops. The infection mainly expends through the leaf hydathodes and injuries, systematically moving through the vascular tissue, which all leads to the formation of necrotic V-shaped lesions. The aim of this study was to gain insight into the genetic diversity of one hundred and twenty two Xcc isolates obtained from six different crucifers (broccoli, cabbage, cauliflower, collard greens, kale and kohlrabi) with black rot symptoms. Samples were collected from different regions in Serbia in 2014. All tested isolates were preliminary identified as Xcc based on morphological and cultural characteristics, pathogenicity, PCR with specific primer set XCF/XCR and gyrB gene sequencing. Study of genetic diversity included two molecular typing methods: pulsed-field gel electrophoresis (PFGE) and repetitive sequence-based PCR (rep-PCR) of repetitive extragenic palindromic (REP-PCR), enterobacterial repetitive intergenic consensus (ERIC-PCR) and BOX elements (BOX-PCR). ERIC- and REP-PCR fingerprinting techniques divided all Xcc isolates into four groups, unlike BOX-PCR and PFGE which yielded same banding patterns for each of the tested isolates. Based on the obtained banding patterns, significant correlation between hosts from which the isolates were obtained, as well as between their geographic distributions was not observed. The results obtained in this work indicate that Xcc population is genetically diverse, which is in correlation with previous findings of genetic diversity within Xcc strains, although it is probably clonal in Serbia, according to PFGE, but additional analysis is required.",
publisher = "UDRUŽENJE MIKROBIOLOGA SRBIJE, Nemanjina 6, Beograd",
journal = "12th Congress of Serbian microbiologists with intenational participation “MICROMED 2018 REGIO”, Belgrade, Serbia",
title = "Genetic characterization of Xanthomonas campestris pv. campestris isolates from different hosts using PFGE and Rep-pcr techniques, Genetička karakterizacija Xanthomonas campestris pv. campestris poreklom sa različitih domaćina upotrebom PFGE i rep-pcr metoda",
pages = "204-203",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2040"
}

Popović Milovanović, T., Jelušić, A., Mitrović, P., Stanković, S., Lozo, J., Nikolić, I.,& Berić, T.. (2018). Genetic characterization of Xanthomonas campestris pv. campestris isolates from different hosts using PFGE and Rep-pcr techniques. in 12th Congress of Serbian microbiologists with intenational participation “MICROMED 2018 REGIO”, Belgrade, Serbia
UDRUŽENJE MIKROBIOLOGA SRBIJE, Nemanjina 6, Beograd., 203-204.
https://hdl.handle.net/21.15107/rcub_rimsi_2040

Popović Milovanović T, Jelušić A, Mitrović P, Stanković S, Lozo J, Nikolić I, Berić T. Genetic characterization of Xanthomonas campestris pv. campestris isolates from different hosts using PFGE and Rep-pcr techniques. in 12th Congress of Serbian microbiologists with intenational participation “MICROMED 2018 REGIO”, Belgrade, Serbia. 2018;:203-204.
https://hdl.handle.net/21.15107/rcub_rimsi_2040 .

Popović Milovanović, Tatjana, Jelušić, Aleksandra, Mitrović, Petar, Stanković, Slaviša, Lozo, Jelena, Nikolić, Ivan, Berić, Tanja, "Genetic characterization of Xanthomonas campestris pv. campestris isolates from different hosts using PFGE and Rep-pcr techniques" in 12th Congress of Serbian microbiologists with intenational participation “MICROMED 2018 REGIO”, Belgrade, Serbia (2018):203-204,
https://hdl.handle.net/21.15107/rcub_rimsi_2040 .