Poleksic, Vesna

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orcid::0000-0001-8612-2962
  • Poleksic, Vesna (10)
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Author's Bibliography

Elemental accumulation and histopathology of two age groups of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia)

Nikolić, Dušan; Smederevac-Lalić, Marija; Skorić, Stefan; Poleksic, Vesna; Raskovic, Bozidar

(Biology Centre of the Czech Academy of Science, Institute of Hydrobiology, Na Sádkách 702/7, České Budějovice, Czech Republic, 2022)

TY  - CONF
AU  - Nikolić, Dušan
AU  - Smederevac-Lalić, Marija
AU  - Skorić, Stefan
AU  - Poleksic, Vesna
AU  - Raskovic, Bozidar
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1668
AB  - Distributed throughout all temperate European inland waters, pikeperch (Sander lucioperca L.) is one of the most interesting fish species for angling and in high demand for human consumption. There is also a growing interest in pikeperch aquaculture. Pikeperch as a freshwater predator has high tendency to accumulate metals in its tissues. Eight individuals of 3+ age class and twelve 4+ individuals were caught at Garaši reservoir during summer of 2017. Concentrations of 26 elements (Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn) were determined in gills, liver and muscles. Metal pollution index (MPI) was used to record the total metal content in different tissues. Apart from this, histopathological lesions in gills and liver were also assessed. Correlation tests between fish condition and element concentration as well as between HP scores for gills and liver were conducted. Aim of the present study was to investigate whether there are differences between element concentrations and HP alterations in the age classes. Results showed that the gills were exposed to the highest pressure of metal pollution in both age classes. There were no significant differences in elemental accumulation and histopathological changes between 3+ and 4+ age classes, nor between the elemental accumulation and condition of fish and between HP indices.
PB  - Biology Centre of the Czech Academy of Science, Institute of Hydrobiology, Na Sádkách 702/7, České Budějovice, Czech Republic
C3  - Percis V 2022 - 5th International Percid Fish Symposium
T1  - Elemental accumulation and histopathology of two age groups of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia)
EP  - 76
SP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1668
ER  - 
@conference{
author = "Nikolić, Dušan and Smederevac-Lalić, Marija and Skorić, Stefan and Poleksic, Vesna and Raskovic, Bozidar",
year = "2022",
abstract = "Distributed throughout all temperate European inland waters, pikeperch (Sander lucioperca L.) is one of the most interesting fish species for angling and in high demand for human consumption. There is also a growing interest in pikeperch aquaculture. Pikeperch as a freshwater predator has high tendency to accumulate metals in its tissues. Eight individuals of 3+ age class and twelve 4+ individuals were caught at Garaši reservoir during summer of 2017. Concentrations of 26 elements (Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn) were determined in gills, liver and muscles. Metal pollution index (MPI) was used to record the total metal content in different tissues. Apart from this, histopathological lesions in gills and liver were also assessed. Correlation tests between fish condition and element concentration as well as between HP scores for gills and liver were conducted. Aim of the present study was to investigate whether there are differences between element concentrations and HP alterations in the age classes. Results showed that the gills were exposed to the highest pressure of metal pollution in both age classes. There were no significant differences in elemental accumulation and histopathological changes between 3+ and 4+ age classes, nor between the elemental accumulation and condition of fish and between HP indices.",
publisher = "Biology Centre of the Czech Academy of Science, Institute of Hydrobiology, Na Sádkách 702/7, České Budějovice, Czech Republic",
journal = "Percis V 2022 - 5th International Percid Fish Symposium",
title = "Elemental accumulation and histopathology of two age groups of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia)",
pages = "76-76",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1668"
}
Nikolić, D., Smederevac-Lalić, M., Skorić, S., Poleksic, V.,& Raskovic, B.. (2022). Elemental accumulation and histopathology of two age groups of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia). in Percis V 2022 - 5th International Percid Fish Symposium
Biology Centre of the Czech Academy of Science, Institute of Hydrobiology, Na Sádkách 702/7, České Budějovice, Czech Republic., 76-76.
https://hdl.handle.net/21.15107/rcub_rimsi_1668
Nikolić D, Smederevac-Lalić M, Skorić S, Poleksic V, Raskovic B. Elemental accumulation and histopathology of two age groups of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia). in Percis V 2022 - 5th International Percid Fish Symposium. 2022;:76-76.
https://hdl.handle.net/21.15107/rcub_rimsi_1668 .
Nikolić, Dušan, Smederevac-Lalić, Marija, Skorić, Stefan, Poleksic, Vesna, Raskovic, Bozidar, "Elemental accumulation and histopathology of two age groups of pikeperch (Sander lucioperca) from Garaši reservoir (Serbia)" in Percis V 2022 - 5th International Percid Fish Symposium (2022):76-76,
https://hdl.handle.net/21.15107/rcub_rimsi_1668 .

Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garasi reservoir (Serbia) with human health risk assessment

Nikolić, Dušan; Skorić, Stefan; Poleksic, Vesna; Rasković, Božidar

(Springer Heidelberg, Heidelberg, 2021)

TY  - JOUR
AU  - Nikolić, Dušan
AU  - Skorić, Stefan
AU  - Poleksic, Vesna
AU  - Rasković, Božidar
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1448
AB  - Accumulation of 26 elements (Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn) was analyzed in the gills, liver, and muscle of pikeperch males and females from Garasi reservoir using inductively coupled plasma optical emission spectrometry (ICP-OES). Histopathological (HP) changes in the gills and liver, and human health risk were also analyzed. The gills were most affected by metal pollution in both sexes. The concentrations of Hg in muscle tissue of four males, and Cd in two females and two males exceeded the maximum allowed concentrations. Statistical tests only revealed significant differences regarding the concentrations of Mg, K, and S in the muscle (higher in males) and Al, Ag, and Mn in the liver (higher in females) of individuals between sexes. Low to moderate levels of pathological changes were recorded for the gills and liver in both sexes. Significant differences between sexes were observed for inflammatory index of gills (I-GI) and HP index of gills (I-G), males had higher values compared to females, and for liver necrosis, where females had higher values compared to males. Gills were less affected by HP changes compared to the liver. There was no significant non-cancerogenic and cancerogenic health risk due to the consumption of pikeperch meat from the Garasi reservoir. However, women are under greater risk by consuming the meat of both male and female individuals, probably due to a longer lifetime and lesser body weight compared to the men.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garasi reservoir (Serbia) with human health risk assessment
EP  - 53711
IS  - 38
SP  - 53700
VL  - 28
DO  - 10.1007/s11356-021-14526-w
ER  - 
@article{
author = "Nikolić, Dušan and Skorić, Stefan and Poleksic, Vesna and Rasković, Božidar",
year = "2021",
abstract = "Accumulation of 26 elements (Ag, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Se, Si, Sr, and Zn) was analyzed in the gills, liver, and muscle of pikeperch males and females from Garasi reservoir using inductively coupled plasma optical emission spectrometry (ICP-OES). Histopathological (HP) changes in the gills and liver, and human health risk were also analyzed. The gills were most affected by metal pollution in both sexes. The concentrations of Hg in muscle tissue of four males, and Cd in two females and two males exceeded the maximum allowed concentrations. Statistical tests only revealed significant differences regarding the concentrations of Mg, K, and S in the muscle (higher in males) and Al, Ag, and Mn in the liver (higher in females) of individuals between sexes. Low to moderate levels of pathological changes were recorded for the gills and liver in both sexes. Significant differences between sexes were observed for inflammatory index of gills (I-GI) and HP index of gills (I-G), males had higher values compared to females, and for liver necrosis, where females had higher values compared to males. Gills were less affected by HP changes compared to the liver. There was no significant non-cancerogenic and cancerogenic health risk due to the consumption of pikeperch meat from the Garasi reservoir. However, women are under greater risk by consuming the meat of both male and female individuals, probably due to a longer lifetime and lesser body weight compared to the men.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garasi reservoir (Serbia) with human health risk assessment",
pages = "53711-53700",
number = "38",
volume = "28",
doi = "10.1007/s11356-021-14526-w"
}
Nikolić, D., Skorić, S., Poleksic, V.,& Rasković, B.. (2021). Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garasi reservoir (Serbia) with human health risk assessment. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 28(38), 53700-53711.
https://doi.org/10.1007/s11356-021-14526-w
Nikolić D, Skorić S, Poleksic V, Rasković B. Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garasi reservoir (Serbia) with human health risk assessment. in Environmental Science and Pollution Research. 2021;28(38):53700-53711.
doi:10.1007/s11356-021-14526-w .
Nikolić, Dušan, Skorić, Stefan, Poleksic, Vesna, Rasković, Božidar, "Sex-specific elemental accumulation and histopathology of pikeperch (Sander lucioperca) from Garasi reservoir (Serbia) with human health risk assessment" in Environmental Science and Pollution Research, 28, no. 38 (2021):53700-53711,
https://doi.org/10.1007/s11356-021-14526-w . .
10
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7

Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia

Kostić-Vuković, Jovana; Kolarević, Stoimir; Kracun-Kolarević, Margareta; Višnjić Jeftić, Željka; Rasković, Božidar; Poleksic, Vesna; Gačić, Zoran; Lenhardt, Mirjana; Vukovic-Gacic, Branka

(Springer, Dordrecht, 2021)

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Višnjić Jeftić, Željka
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1501
AB  - This study was conducted on the Danube River locality Visnjica, exposed to the discharge of the largest wastewater collector in Serbia's capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia
IS  - 8
VL  - 193
DO  - 10.1007/s10661-021-09232-6
ER  - 
@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Višnjić Jeftić, Željka and Rasković, Božidar and Poleksic, Vesna and Gačić, Zoran and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2021",
abstract = "This study was conducted on the Danube River locality Visnjica, exposed to the discharge of the largest wastewater collector in Serbia's capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia",
number = "8",
volume = "193",
doi = "10.1007/s10661-021-09232-6"
}
Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Višnjić Jeftić, Ž., Rasković, B., Poleksic, V., Gačić, Z., Lenhardt, M.,& Vukovic-Gacic, B.. (2021). Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment
Springer, Dordrecht., 193(8).
https://doi.org/10.1007/s10661-021-09232-6
Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Višnjić Jeftić Ž, Rasković B, Poleksic V, Gačić Z, Lenhardt M, Vukovic-Gacic B. Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment. 2021;193(8).
doi:10.1007/s10661-021-09232-6 .
Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Višnjić Jeftić, Željka, Rasković, Božidar, Poleksic, Vesna, Gačić, Zoran, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia" in Environmental Monitoring and Assessment, 193, no. 8 (2021),
https://doi.org/10.1007/s10661-021-09232-6 . .
10
1
7

Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia

Rasković, Božidar; Poleksic, Vesna; Skorić, Stefan; Jovicic, Katarina; Spasić, Slađana; Hegediš, Aleksandar; Vasic, Nebojša; Lenhardt, Mirjana

(Academic Press Inc Elsevier Science, San Diego, 2018)

TY  - JOUR
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Skorić, Stefan
AU  - Jovicic, Katarina
AU  - Spasić, Slađana
AU  - Hegediš, Aleksandar
AU  - Vasic, Nebojša
AU  - Lenhardt, Mirjana
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1120
AB  - Chub (Squalius cephalus) specimens were collected in Korenita River seven months after spillover from the waste water of antimony mine tailing pond and compared with chub living in Kruscica reservoir (intended for water supply) and Meduvrsje reservoir (influenced by intense emission of industrial, urban and rural wastewater). Concentrations of 15 elements (Al, As, B, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Si, Sr, Zn) were determined in muscle, liver and gills of chub by inductively coupled plasma optical spectrometry (ICP-OES) and histopathological alterations in liver and gills were assessed. Chub specimens from Korenita River had higher concentrations of As, Ba and Pb in all three investigated tissues as well as higher total histopathological index values than chub from reservoirs. Specimens from Meduvrsje reservoir were characterized by higher values for concentrations of Cu and Si in muscle tissue and higher values for regressive histopathological alterations in gills. Individuals of chub from Kruscica reservoir had the highest concentrations of Fe in liver, Hg in muscle and Sr and Zn in muscle while gills had the lowest value of total histopathological index. The results from the present study showed higher level of histopathological alterations as a result of mine tailing accident. As a result of mixed contamination on the Meduvrsje site, histopathological index values of gills were in line with the index value from Korenita River. Increased values for Fe and Sr in chub tissue from Kruscica reservoir could be explained by geological structure of the site which is characterized by magmatic rock rich in Cu, Fe and Ni as well as dominant carbonate sediment complex of marine origin with increased level of Sr.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Ecotoxicology and Environmental Safety
T1  - Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia
EP  - 247
SP  - 238
VL  - 153
DO  - 10.1016/j.ecoenv.2018.01.058
ER  - 
@article{
author = "Rasković, Božidar and Poleksic, Vesna and Skorić, Stefan and Jovicic, Katarina and Spasić, Slađana and Hegediš, Aleksandar and Vasic, Nebojša and Lenhardt, Mirjana",
year = "2018",
abstract = "Chub (Squalius cephalus) specimens were collected in Korenita River seven months after spillover from the waste water of antimony mine tailing pond and compared with chub living in Kruscica reservoir (intended for water supply) and Meduvrsje reservoir (influenced by intense emission of industrial, urban and rural wastewater). Concentrations of 15 elements (Al, As, B, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Si, Sr, Zn) were determined in muscle, liver and gills of chub by inductively coupled plasma optical spectrometry (ICP-OES) and histopathological alterations in liver and gills were assessed. Chub specimens from Korenita River had higher concentrations of As, Ba and Pb in all three investigated tissues as well as higher total histopathological index values than chub from reservoirs. Specimens from Meduvrsje reservoir were characterized by higher values for concentrations of Cu and Si in muscle tissue and higher values for regressive histopathological alterations in gills. Individuals of chub from Kruscica reservoir had the highest concentrations of Fe in liver, Hg in muscle and Sr and Zn in muscle while gills had the lowest value of total histopathological index. The results from the present study showed higher level of histopathological alterations as a result of mine tailing accident. As a result of mixed contamination on the Meduvrsje site, histopathological index values of gills were in line with the index value from Korenita River. Increased values for Fe and Sr in chub tissue from Kruscica reservoir could be explained by geological structure of the site which is characterized by magmatic rock rich in Cu, Fe and Ni as well as dominant carbonate sediment complex of marine origin with increased level of Sr.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Ecotoxicology and Environmental Safety",
title = "Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia",
pages = "247-238",
volume = "153",
doi = "10.1016/j.ecoenv.2018.01.058"
}
Rasković, B., Poleksic, V., Skorić, S., Jovicic, K., Spasić, S., Hegediš, A., Vasic, N.,& Lenhardt, M.. (2018). Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia. in Ecotoxicology and Environmental Safety
Academic Press Inc Elsevier Science, San Diego., 153, 238-247.
https://doi.org/10.1016/j.ecoenv.2018.01.058
Rasković B, Poleksic V, Skorić S, Jovicic K, Spasić S, Hegediš A, Vasic N, Lenhardt M. Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia. in Ecotoxicology and Environmental Safety. 2018;153:238-247.
doi:10.1016/j.ecoenv.2018.01.058 .
Rasković, Božidar, Poleksic, Vesna, Skorić, Stefan, Jovicic, Katarina, Spasić, Slađana, Hegediš, Aleksandar, Vasic, Nebojša, Lenhardt, Mirjana, "Effects of mine tailing and mixed contamination on metals, trace elements accumulation and histopathology of the chub (Squalius cephalus) tissues: Evidence from three differently contaminated sites in Serbia" in Ecotoxicology and Environmental Safety, 153 (2018):238-247,
https://doi.org/10.1016/j.ecoenv.2018.01.058 . .
1
24
13
22

The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons

Kostić-Vuković, Jovana; Kolarević, Stoimir; Kracun-Kolarević, Margareta; Aborgiba, Mustafa; Gačić, Zoran; Paunović, Momir; Višnjić Jeftić, Željka; Rasković, Božidar; Poleksic, Vesna; Lenhardt, Mirjana; Vukovic-Gacic, Branka

(Elsevier Science Bv, Amsterdam, 2017)

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Višnjić Jeftić, Željka
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1060
AB  - Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons
EP  - 1681
SP  - 1670
VL  - 601
DO  - 10.1016/j.scitotenv.2017.05.273
ER  - 
@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Aborgiba, Mustafa and Gačić, Zoran and Paunović, Momir and Višnjić Jeftić, Željka and Rasković, Božidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2017",
abstract = "Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons",
pages = "1681-1670",
volume = "601",
doi = "10.1016/j.scitotenv.2017.05.273"
}
Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Aborgiba, M., Gačić, Z., Paunović, M., Višnjić Jeftić, Ž., Rasković, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2017). The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 601, 1670-1681.
https://doi.org/10.1016/j.scitotenv.2017.05.273
Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Aborgiba M, Gačić Z, Paunović M, Višnjić Jeftić Ž, Rasković B, Poleksic V, Lenhardt M, Vukovic-Gacic B. The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment. 2017;601:1670-1681.
doi:10.1016/j.scitotenv.2017.05.273 .
Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Aborgiba, Mustafa, Gačić, Zoran, Paunović, Momir, Višnjić Jeftić, Željka, Rasković, Božidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons" in Science of the Total Environment, 601 (2017):1670-1681,
https://doi.org/10.1016/j.scitotenv.2017.05.273 . .
3
46
26
48

Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution

Rasković, Božidar; Poleksic, Vesna; Višnjić Jeftić, Željka; Skorić, Stefan; Gačić, Zoran; Đikanović, Vesna; Jarić, Ivan; Lenhardt, Mirjana

(Wiley, Hoboken, 2015)

TY  - JOUR
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Višnjić Jeftić, Željka
AU  - Skorić, Stefan
AU  - Gačić, Zoran
AU  - Đikanović, Vesna
AU  - Jarić, Ivan
AU  - Lenhardt, Mirjana
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/864
AB  - The bottom feeding fish species have a good potential to be used for assessments of pollution, as they are under pressure from pollutants from both water and sediments. In this study, the level of similarity of histopathological responses to pollution in gills and liver between barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube River was assessed, and compared with elemental concentrations in their gills, liver, and muscle. Results indicate that the detected metal concentrations were likely cause of different tissue responses in gills and liver of the two investigated fish species. Statistical analysis indicated a clear differentiation of the two species based on elemental concentrations and the level of histopathological changes in gills and liver. Metal concentrations exceeded maximum acceptable concentrations in a number of analyzed specimens, which indicates the importance of this type of monitoring. Results indicate that barbel is a better indicator for specific, rather narrow sites, whereas sterlet is a better indicator of larger (longer) water current segments. Obtained information could be of importance for both scientists and fishery and water management authorities working on the development of water monitoring programs.
PB  - Wiley, Hoboken
T2  - Environmental Toxicology
T1  - Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution
EP  - 1161
IS  - 10
SP  - 1153
VL  - 30
DO  - 10.1002/tox.21988
ER  - 
@article{
author = "Rasković, Božidar and Poleksic, Vesna and Višnjić Jeftić, Željka and Skorić, Stefan and Gačić, Zoran and Đikanović, Vesna and Jarić, Ivan and Lenhardt, Mirjana",
year = "2015",
abstract = "The bottom feeding fish species have a good potential to be used for assessments of pollution, as they are under pressure from pollutants from both water and sediments. In this study, the level of similarity of histopathological responses to pollution in gills and liver between barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube River was assessed, and compared with elemental concentrations in their gills, liver, and muscle. Results indicate that the detected metal concentrations were likely cause of different tissue responses in gills and liver of the two investigated fish species. Statistical analysis indicated a clear differentiation of the two species based on elemental concentrations and the level of histopathological changes in gills and liver. Metal concentrations exceeded maximum acceptable concentrations in a number of analyzed specimens, which indicates the importance of this type of monitoring. Results indicate that barbel is a better indicator for specific, rather narrow sites, whereas sterlet is a better indicator of larger (longer) water current segments. Obtained information could be of importance for both scientists and fishery and water management authorities working on the development of water monitoring programs.",
publisher = "Wiley, Hoboken",
journal = "Environmental Toxicology",
title = "Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution",
pages = "1161-1153",
number = "10",
volume = "30",
doi = "10.1002/tox.21988"
}
Rasković, B., Poleksic, V., Višnjić Jeftić, Ž., Skorić, S., Gačić, Z., Đikanović, V., Jarić, I.,& Lenhardt, M.. (2015). Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution. in Environmental Toxicology
Wiley, Hoboken., 30(10), 1153-1161.
https://doi.org/10.1002/tox.21988
Rasković B, Poleksic V, Višnjić Jeftić Ž, Skorić S, Gačić Z, Đikanović V, Jarić I, Lenhardt M. Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution. in Environmental Toxicology. 2015;30(10):1153-1161.
doi:10.1002/tox.21988 .
Rasković, Božidar, Poleksic, Vesna, Višnjić Jeftić, Željka, Skorić, Stefan, Gačić, Zoran, Đikanović, Vesna, Jarić, Ivan, Lenhardt, Mirjana, "Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution" in Environmental Toxicology, 30, no. 10 (2015):1153-1161,
https://doi.org/10.1002/tox.21988 . .
34
22
30

Integrated use of different fish related parameters to assess the status of water bodies

Lenhardt, Mirjana; Poleksic, Vesna; Vukovic-Gacic, Branka; Rasković, Božidar; Sunjog, Karolina; Kolarević, Stoimir; Jarić, Ivan; Gačić, Zoran

(University of Ljubljana - Veterinary Faculty, 2015)

TY  - JOUR
AU  - Lenhardt, Mirjana
AU  - Poleksic, Vesna
AU  - Vukovic-Gacic, Branka
AU  - Rasković, Božidar
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Jarić, Ivan
AU  - Gačić, Zoran
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/908
AB  - Freshwater bodies receive high levels of different toxicants. Since fish are typically situated at the top of aquatic food chains, they have a good potential to be used as indicators of water pollution. Assessment of the presence of pollutants and their toxicity can be efficiently performed by the combined use of analytical chemistry, bioassays and applied mathematics. In this study, we present the general approach of the integrated use of different fish related parameters to assess the status and pollution levels of waterbodies. We discuss our previous experiences within the field of analytical toxicology, toxicological pathology and toxicity testing, as well as biomathematical and statistical methods that are able to provide for integration of results acquired by each of the specific methods. We discuss advantages and shortcomings of each of the methods, and present necessary future steps in the method development. Since the industrial and domestic wastewaters in Serbia are still not processed before being released into watercourses, they pose a serious risk for aquatic ecosystems and public health. Consequently, described pollution indicators and genotoxicity parameters represent an essential tool for efficient monitoring of aquatic ecosystems. Methodological approach presented here might be of interest for scientists and managers dealing with the ecotoxicological research and monitoring of freshwater ecosystems.
PB  - University of Ljubljana - Veterinary Faculty
T2  - Slovenian Veterinary Research
T1  - Integrated use of different fish related parameters to assess the status of water bodies
EP  - 13
IS  - 1
SP  - 5
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3781
ER  - 
@article{
author = "Lenhardt, Mirjana and Poleksic, Vesna and Vukovic-Gacic, Branka and Rasković, Božidar and Sunjog, Karolina and Kolarević, Stoimir and Jarić, Ivan and Gačić, Zoran",
year = "2015",
abstract = "Freshwater bodies receive high levels of different toxicants. Since fish are typically situated at the top of aquatic food chains, they have a good potential to be used as indicators of water pollution. Assessment of the presence of pollutants and their toxicity can be efficiently performed by the combined use of analytical chemistry, bioassays and applied mathematics. In this study, we present the general approach of the integrated use of different fish related parameters to assess the status and pollution levels of waterbodies. We discuss our previous experiences within the field of analytical toxicology, toxicological pathology and toxicity testing, as well as biomathematical and statistical methods that are able to provide for integration of results acquired by each of the specific methods. We discuss advantages and shortcomings of each of the methods, and present necessary future steps in the method development. Since the industrial and domestic wastewaters in Serbia are still not processed before being released into watercourses, they pose a serious risk for aquatic ecosystems and public health. Consequently, described pollution indicators and genotoxicity parameters represent an essential tool for efficient monitoring of aquatic ecosystems. Methodological approach presented here might be of interest for scientists and managers dealing with the ecotoxicological research and monitoring of freshwater ecosystems.",
publisher = "University of Ljubljana - Veterinary Faculty",
journal = "Slovenian Veterinary Research",
title = "Integrated use of different fish related parameters to assess the status of water bodies",
pages = "13-5",
number = "1",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3781"
}
Lenhardt, M., Poleksic, V., Vukovic-Gacic, B., Rasković, B., Sunjog, K., Kolarević, S., Jarić, I.,& Gačić, Z.. (2015). Integrated use of different fish related parameters to assess the status of water bodies. in Slovenian Veterinary Research
University of Ljubljana - Veterinary Faculty., 52(1), 5-13.
https://hdl.handle.net/21.15107/rcub_agrospace_3781
Lenhardt M, Poleksic V, Vukovic-Gacic B, Rasković B, Sunjog K, Kolarević S, Jarić I, Gačić Z. Integrated use of different fish related parameters to assess the status of water bodies. in Slovenian Veterinary Research. 2015;52(1):5-13.
https://hdl.handle.net/21.15107/rcub_agrospace_3781 .
Lenhardt, Mirjana, Poleksic, Vesna, Vukovic-Gacic, Branka, Rasković, Božidar, Sunjog, Karolina, Kolarević, Stoimir, Jarić, Ivan, Gačić, Zoran, "Integrated use of different fish related parameters to assess the status of water bodies" in Slovenian Veterinary Research, 52, no. 1 (2015):5-13,
https://hdl.handle.net/21.15107/rcub_agrospace_3781 .
8
8

Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices

Skorić, Stefan; Rasković, Božidar; Poleksic, Vesna; Gačić, Zoran; Lenhardt, Mirjana

(Springer, Dordrecht, 2012)

TY  - JOUR
AU  - Skorić, Stefan
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/546
AB  - Cormorants cause damage to fish in aquaculture not only by consuming large number of fish but also by wounding them. Quantification of the actual damage in fish induced by wounding is important for reliable estimations of the overall loss in aquaculture caused by cormorants. In this study, specimens of mirror and scaly carp ( L.) injured by attacks of great cormorant ( L.) were collected from the "Eka" fish farm in Serbia. Surfaces of superficial and open subepithelial wounds (expressed as a percent of the total body surface), estimated by computer-assisted image analysis, had mean values 10.66 +/- A 1.86 and 0.85 +/- A 0.90, respectively. Histological evaluation of wounds was performed by two scoring systems: system proposed by Bernet et al. (J Fish Dis 22:25-34, 1999) and modified system described by Wahli et al. (Aquaculture 225:371-386, 2003). The most frequent histopathological changes were disturbed epidermal structure and surface cell morphology and increased number of club cells (on more than 75% of specimens); increased number of melanocytes and their distribution in different layers in dermis; and enlarged hypodermis (more than 80% of samples) and myolysis (50% of samples). Comparison of wounds' surfaces (surface of superficial injures) and histopathological changes, estimated by two scoring systems, revealed significant positive correlation. Bernet's scoring system showed higher correlation coefficients, when compared with the modified Wahli's scoring system.
PB  - Springer, Dordrecht
T2  - Aquaculture International
T1  - Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices
EP  - 535
IS  - 3
SP  - 525
VL  - 20
DO  - 10.1007/s10499-011-9483-3
ER  - 
@article{
author = "Skorić, Stefan and Rasković, Božidar and Poleksic, Vesna and Gačić, Zoran and Lenhardt, Mirjana",
year = "2012",
abstract = "Cormorants cause damage to fish in aquaculture not only by consuming large number of fish but also by wounding them. Quantification of the actual damage in fish induced by wounding is important for reliable estimations of the overall loss in aquaculture caused by cormorants. In this study, specimens of mirror and scaly carp ( L.) injured by attacks of great cormorant ( L.) were collected from the "Eka" fish farm in Serbia. Surfaces of superficial and open subepithelial wounds (expressed as a percent of the total body surface), estimated by computer-assisted image analysis, had mean values 10.66 +/- A 1.86 and 0.85 +/- A 0.90, respectively. Histological evaluation of wounds was performed by two scoring systems: system proposed by Bernet et al. (J Fish Dis 22:25-34, 1999) and modified system described by Wahli et al. (Aquaculture 225:371-386, 2003). The most frequent histopathological changes were disturbed epidermal structure and surface cell morphology and increased number of club cells (on more than 75% of specimens); increased number of melanocytes and their distribution in different layers in dermis; and enlarged hypodermis (more than 80% of samples) and myolysis (50% of samples). Comparison of wounds' surfaces (surface of superficial injures) and histopathological changes, estimated by two scoring systems, revealed significant positive correlation. Bernet's scoring system showed higher correlation coefficients, when compared with the modified Wahli's scoring system.",
publisher = "Springer, Dordrecht",
journal = "Aquaculture International",
title = "Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices",
pages = "535-525",
number = "3",
volume = "20",
doi = "10.1007/s10499-011-9483-3"
}
Skorić, S., Rasković, B., Poleksic, V., Gačić, Z.,& Lenhardt, M.. (2012). Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices. in Aquaculture International
Springer, Dordrecht., 20(3), 525-535.
https://doi.org/10.1007/s10499-011-9483-3
Skorić S, Rasković B, Poleksic V, Gačić Z, Lenhardt M. Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices. in Aquaculture International. 2012;20(3):525-535.
doi:10.1007/s10499-011-9483-3 .
Skorić, Stefan, Rasković, Božidar, Poleksic, Vesna, Gačić, Zoran, Lenhardt, Mirjana, "Scoring of the extent and intensity of carp (Cyprinus Carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices" in Aquaculture International, 20, no. 3 (2012):525-535,
https://doi.org/10.1007/s10499-011-9483-3 . .
3
2
3

Ecological status of Serbian rivers based on an ichthyological assessment

Lenhardt, Mirjana; Gačić, Zoran; Vukovic-Gacic, Branka; Poleksic, Vesna; Višnjić Jeftić, Željka; Kolarević, Stoimir; Jarić, Ivan

(Vasile Goldis Western University, Arad, 2011)

TY  - JOUR
AU  - Lenhardt, Mirjana
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
AU  - Poleksic, Vesna
AU  - Višnjić Jeftić, Željka
AU  - Kolarević, Stoimir
AU  - Jarić, Ivan
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/445
AB  - Anthropogenic influences on fish populations in river ecosystems can be studied at the individual level, as well as through the assessment of fish fauna composition. This study presents research that was focused at the individual level, such as the analysis of genotoxic effects (Comet assay), assessment of the heavy metal accumulation in fish liver, gills, intestine and muscle, histopathological analysis of liver, gills and skin, presence of parasites and the level of parasite infestation, hepatosomatic index and the condition factor. At the level of fish fauna composition, monitoring of the changes caused by dam construction was conducted through the use of modified index of biotic integrity (IBI). The aim of this work was to analyze all above listed parameters as potential bioindicators that can be utilized to assess the extent of disturbance of river systems in Serbia by anthropogenic impacts.
PB  - Vasile Goldis Western University, Arad
T2  - Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii
T1  - Ecological status of Serbian rivers based on an ichthyological assessment
EP  - 860
IS  - 4
SP  - 855
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2414
ER  - 
@article{
author = "Lenhardt, Mirjana and Gačić, Zoran and Vukovic-Gacic, Branka and Poleksic, Vesna and Višnjić Jeftić, Željka and Kolarević, Stoimir and Jarić, Ivan",
year = "2011",
abstract = "Anthropogenic influences on fish populations in river ecosystems can be studied at the individual level, as well as through the assessment of fish fauna composition. This study presents research that was focused at the individual level, such as the analysis of genotoxic effects (Comet assay), assessment of the heavy metal accumulation in fish liver, gills, intestine and muscle, histopathological analysis of liver, gills and skin, presence of parasites and the level of parasite infestation, hepatosomatic index and the condition factor. At the level of fish fauna composition, monitoring of the changes caused by dam construction was conducted through the use of modified index of biotic integrity (IBI). The aim of this work was to analyze all above listed parameters as potential bioindicators that can be utilized to assess the extent of disturbance of river systems in Serbia by anthropogenic impacts.",
publisher = "Vasile Goldis Western University, Arad",
journal = "Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii",
title = "Ecological status of Serbian rivers based on an ichthyological assessment",
pages = "860-855",
number = "4",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2414"
}
Lenhardt, M., Gačić, Z., Vukovic-Gacic, B., Poleksic, V., Višnjić Jeftić, Ž., Kolarević, S.,& Jarić, I.. (2011). Ecological status of Serbian rivers based on an ichthyological assessment. in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii
Vasile Goldis Western University, Arad., 21(4), 855-860.
https://hdl.handle.net/21.15107/rcub_agrospace_2414
Lenhardt M, Gačić Z, Vukovic-Gacic B, Poleksic V, Višnjić Jeftić Ž, Kolarević S, Jarić I. Ecological status of Serbian rivers based on an ichthyological assessment. in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii. 2011;21(4):855-860.
https://hdl.handle.net/21.15107/rcub_agrospace_2414 .
Lenhardt, Mirjana, Gačić, Zoran, Vukovic-Gacic, Branka, Poleksic, Vesna, Višnjić Jeftić, Željka, Kolarević, Stoimir, Jarić, Ivan, "Ecological status of Serbian rivers based on an ichthyological assessment" in Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii, 21, no. 4 (2011):855-860,
https://hdl.handle.net/21.15107/rcub_agrospace_2414 .
4

Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758)

Poleksic, Vesna; Lenhardt, Mirjana; Jarić, Ivan; Đorđević, Dragana S; Gačić, Zoran; Cvijanović, Gorčin; Rasković, Božidar

(Setac Press, Pensacola, 2010)

TY  - JOUR
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Jarić, Ivan
AU  - Đorđević, Dragana S
AU  - Gačić, Zoran
AU  - Cvijanović, Gorčin
AU  - Rasković, Božidar
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/388
AB  - The sterlet (Acipenser ruthenus L.) is a bottom-feeding fish species with a direct exposure to contaminants from water and sediments. Although heavy metal pollution is believed to be one of the main threats to the sterlet population in the Danube River basin, there is a lack of knowledge of the exact impact of heavy metals on their survival. In the present study, effects of heavy metal pollution on sterlet in the Danube basin were assessed as well as the utility of different sterlet organs and tissues as indicators of heavy metal contamination. The sterlet were sampled at three different sites in the Danube basin, in Hungary and Serbia, isolated from each other by dams. Heavy metal analysis included measurement of Cd, As, Pb, Cr, Hg, Cu, Ni, Fe, Mn, and Zn concentrations in sterlet gills, muscle, liver, and intestine, and histopathological analyses comprised assessment and scoring of the extent and intensity of alterations in skin, gills, and liver tissue. Analysis revealed a significant presence of sublethal histopathological changes that were most pronounced in the liver and skin and increased accumulation of heavy metals, with the highest concentrations in the liver. Canonical discriminant analysis showed significant differentiation among the three studied localities, suggesting that the heavy metal concentrations in sterlet populations were site specific. The present study concludes that the accumulation of heavy metals is a response to the presence of these pollutants in the environment, and, together with other pollutants, it affects the vital organs of natural sterlet populations.
PB  - Setac Press, Pensacola
T2  - Environmental Toxicology and Chemistry
T1  - Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758)
EP  - 521
IS  - 3
SP  - 515
VL  - 29
DO  - 10.1002/etc.82
ER  - 
@article{
author = "Poleksic, Vesna and Lenhardt, Mirjana and Jarić, Ivan and Đorđević, Dragana S and Gačić, Zoran and Cvijanović, Gorčin and Rasković, Božidar",
year = "2010",
abstract = "The sterlet (Acipenser ruthenus L.) is a bottom-feeding fish species with a direct exposure to contaminants from water and sediments. Although heavy metal pollution is believed to be one of the main threats to the sterlet population in the Danube River basin, there is a lack of knowledge of the exact impact of heavy metals on their survival. In the present study, effects of heavy metal pollution on sterlet in the Danube basin were assessed as well as the utility of different sterlet organs and tissues as indicators of heavy metal contamination. The sterlet were sampled at three different sites in the Danube basin, in Hungary and Serbia, isolated from each other by dams. Heavy metal analysis included measurement of Cd, As, Pb, Cr, Hg, Cu, Ni, Fe, Mn, and Zn concentrations in sterlet gills, muscle, liver, and intestine, and histopathological analyses comprised assessment and scoring of the extent and intensity of alterations in skin, gills, and liver tissue. Analysis revealed a significant presence of sublethal histopathological changes that were most pronounced in the liver and skin and increased accumulation of heavy metals, with the highest concentrations in the liver. Canonical discriminant analysis showed significant differentiation among the three studied localities, suggesting that the heavy metal concentrations in sterlet populations were site specific. The present study concludes that the accumulation of heavy metals is a response to the presence of these pollutants in the environment, and, together with other pollutants, it affects the vital organs of natural sterlet populations.",
publisher = "Setac Press, Pensacola",
journal = "Environmental Toxicology and Chemistry",
title = "Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758)",
pages = "521-515",
number = "3",
volume = "29",
doi = "10.1002/etc.82"
}
Poleksic, V., Lenhardt, M., Jarić, I., Đorđević, D. S., Gačić, Z., Cvijanović, G.,& Rasković, B.. (2010). Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758). in Environmental Toxicology and Chemistry
Setac Press, Pensacola., 29(3), 515-521.
https://doi.org/10.1002/etc.82
Poleksic V, Lenhardt M, Jarić I, Đorđević DS, Gačić Z, Cvijanović G, Rasković B. Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758). in Environmental Toxicology and Chemistry. 2010;29(3):515-521.
doi:10.1002/etc.82 .
Poleksic, Vesna, Lenhardt, Mirjana, Jarić, Ivan, Đorđević, Dragana S, Gačić, Zoran, Cvijanović, Gorčin, Rasković, Božidar, "Liver, gills, and skin histopathology and heavy metal content of the Danube sterlet (acipenser ruthenus linnaeus, 1758)" in Environmental Toxicology and Chemistry, 29, no. 3 (2010):515-521,
https://doi.org/10.1002/etc.82 . .
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103