TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Ducic, Tanja
AU  - Žižić, Milan
AU  - Dimitrijević, Milena
AU  - Danilović Luković, Jelena
AU  - Milenković, Milica R.
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1354
AB  - Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.
PB  - Portland Press Ltd, London
T2  - Biochemical Journal
T1  - Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana
EP  - 3741
IS  - 19
SP  - 3729
VL  - 477
DO  - 10.1042/BCJ20200600
ER  - 

@article{
author = "Vojvodić, Snežana and Stanić, Marina and Zechmann, Bernd and Ducic, Tanja and Žižić, Milan and Dimitrijević, Milena and Danilović Luković, Jelena and Milenković, Milica R. and Pittman, Jon K. and Spasojević, Ivan",
year = "2020",
abstract = "Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.",
publisher = "Portland Press Ltd, London",
journal = "Biochemical Journal",
title = "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana",
pages = "3741-3729",
number = "19",
volume = "477",
doi = "10.1042/BCJ20200600"
}

Vojvodić, S., Stanić, M., Zechmann, B., Ducic, T., Žižić, M., Dimitrijević, M., Danilović Luković, J., Milenković, M. R., Pittman, J. K.,& Spasojević, I.. (2020). Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal
Portland Press Ltd, London., 477(19), 3729-3741.
https://doi.org/10.1042/BCJ20200600

Vojvodić S, Stanić M, Zechmann B, Ducic T, Žižić M, Dimitrijević M, Danilović Luković J, Milenković MR, Pittman JK, Spasojević I. Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal. 2020;477(19):3729-3741.
doi:10.1042/BCJ20200600 .

Vojvodić, Snežana, Stanić, Marina, Zechmann, Bernd, Ducic, Tanja, Žižić, Milan, Dimitrijević, Milena, Danilović Luković, Jelena, Milenković, Milica R., Pittman, Jon K., Spasojević, Ivan, "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana" in Biochemical Journal, 477, no. 19 (2020):3729-3741,
https://doi.org/10.1042/BCJ20200600 . .

TY  - JOUR
AU  - Marjanović, Žaklina
AU  - Grebenc, T.
AU  - Marković, M.
AU  - Glišić, A.
AU  - Milenković, Milica R.
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/391
AB  - Marjanovic, Z(1), Grebenc, T(2), Markovic, M(3), Glisic, A(4), and Milenkovic, M(5) (2010) Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula Sydowia 62 (1) 67-87 Little is known on the diversity and distribution of truffles at the Balkan Peninsula A first detailed study of hypogeous fungi in Serbia has been started at 1992 To extend the knowledge on European truffles and their natural habitats, data on their diversity in Serbia, and to some extent in Montenegro and FYRO Macedonia are summarised within this paper Twelve species of the genus Tuber, including five varieties of T rufum Pico, are recorded and their habitats briefly described Four species are reported for the Balkan Peninsula for the first time In addition, parts of ribosomal DNA (ITS regions) of 46 specimens are sequenced and analysed to confirm the morphological determinations ITS sequences of specimens morphologically identified as Tuber fulgens Quel are reported for the first time A phylogeny based on ITS sequences of the Balkan truffles and additional 29 records from the GenBank is calculated The results are discussed towards expanding the information on distribution as well as ecological and molecular diversification of Tuber spp in Europe
T2  - Sydowia
T1  - Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula
EP  - 87
IS  - 1
SP  - 67
VL  - 62
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_391
ER  - 

@article{
author = "Marjanović, Žaklina and Grebenc, T. and Marković, M. and Glišić, A. and Milenković, Milica R.",
year = "2010",
abstract = "Marjanovic, Z(1), Grebenc, T(2), Markovic, M(3), Glisic, A(4), and Milenkovic, M(5) (2010) Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula Sydowia 62 (1) 67-87 Little is known on the diversity and distribution of truffles at the Balkan Peninsula A first detailed study of hypogeous fungi in Serbia has been started at 1992 To extend the knowledge on European truffles and their natural habitats, data on their diversity in Serbia, and to some extent in Montenegro and FYRO Macedonia are summarised within this paper Twelve species of the genus Tuber, including five varieties of T rufum Pico, are recorded and their habitats briefly described Four species are reported for the Balkan Peninsula for the first time In addition, parts of ribosomal DNA (ITS regions) of 46 specimens are sequenced and analysed to confirm the morphological determinations ITS sequences of specimens morphologically identified as Tuber fulgens Quel are reported for the first time A phylogeny based on ITS sequences of the Balkan truffles and additional 29 records from the GenBank is calculated The results are discussed towards expanding the information on distribution as well as ecological and molecular diversification of Tuber spp in Europe",
journal = "Sydowia",
title = "Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula",
pages = "87-67",
number = "1",
volume = "62",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_391"
}

Marjanović, Ž., Grebenc, T., Marković, M., Glišić, A.,& Milenković, M. R.. (2010). Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula. in Sydowia, 62(1), 67-87.
https://hdl.handle.net/21.15107/rcub_rimsi_391

Marjanović Ž, Grebenc T, Marković M, Glišić A, Milenković MR. Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula. in Sydowia. 2010;62(1):67-87.
https://hdl.handle.net/21.15107/rcub_rimsi_391 .

Marjanović, Žaklina, Grebenc, T., Marković, M., Glišić, A., Milenković, Milica R., "Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula" in Sydowia, 62, no. 1 (2010):67-87,
https://hdl.handle.net/21.15107/rcub_rimsi_391 .