TY  - JOUR
AU  - Zeng, Fanrong
AU  - Shabala, Sergey
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Bonales-Alatorre, Edgar
AU  - Shabala, Lana
AU  - Yu, Min
AU  - Zhang, Guoping
AU  - Živanović, Branka  D.
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1173
AB  - Efforts to breed salt tolerant crops could benefit from investigating previously unexplored traits. One of them is a tissue succulency. In this work, we have undertaken an electrophysiological and biochemical comparison of properties of mesophyll and storage parenchyma leaf tissues of a succulent halophyte species Carpobrotus rosii ("pigface"). We show that storage parenchyma cells of C. rossii act as Na+ sink and possessed both higher Na+ sequestration (298 vs. 215 mM NaCl in mesophyll) and better K+ retention ability. The latter traits was determined by the higher rate of H+-ATPase operation and higher nonenzymatic antioxidant activity in this tissue. Na+ uptake in both tissues was insensitive to either Gd3+ or elevated Ca2+ ruling out involvement of nonselective cation channels as a major path for Na+ entry. Patch-clamp experiments have revealed that Caprobrotus plants were capable to downregulate activity of fast vacuolar channels when exposed to saline environment; this ability was higher in the storage parenchyma cells compared with mesophyll. Also, storage parenchyma cells have constitutively lower number of open slow vacuolar channels, whereas in mesophyll, this suppression was inducible by salt. Taken together, these results provide a mechanistic basis for efficient Na+ sequestration in the succulent leaf tissues.
PB  - Wiley, Hoboken
T2  - Plant Cell and Environment
T1  - Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi
EP  - 2667
IS  - 11
SP  - 2654
VL  - 41
DO  - 10.1111/pce.13391
ER  - 

@article{
author = "Zeng, Fanrong and Shabala, Sergey and Dragišić Maksimović, Jelena and Maksimović, Vuk and Bonales-Alatorre, Edgar and Shabala, Lana and Yu, Min and Zhang, Guoping and Živanović, Branka  D.",
year = "2018",
abstract = "Efforts to breed salt tolerant crops could benefit from investigating previously unexplored traits. One of them is a tissue succulency. In this work, we have undertaken an electrophysiological and biochemical comparison of properties of mesophyll and storage parenchyma leaf tissues of a succulent halophyte species Carpobrotus rosii ("pigface"). We show that storage parenchyma cells of C. rossii act as Na+ sink and possessed both higher Na+ sequestration (298 vs. 215 mM NaCl in mesophyll) and better K+ retention ability. The latter traits was determined by the higher rate of H+-ATPase operation and higher nonenzymatic antioxidant activity in this tissue. Na+ uptake in both tissues was insensitive to either Gd3+ or elevated Ca2+ ruling out involvement of nonselective cation channels as a major path for Na+ entry. Patch-clamp experiments have revealed that Caprobrotus plants were capable to downregulate activity of fast vacuolar channels when exposed to saline environment; this ability was higher in the storage parenchyma cells compared with mesophyll. Also, storage parenchyma cells have constitutively lower number of open slow vacuolar channels, whereas in mesophyll, this suppression was inducible by salt. Taken together, these results provide a mechanistic basis for efficient Na+ sequestration in the succulent leaf tissues.",
publisher = "Wiley, Hoboken",
journal = "Plant Cell and Environment",
title = "Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi",
pages = "2667-2654",
number = "11",
volume = "41",
doi = "10.1111/pce.13391"
}

Zeng, F., Shabala, S., Dragišić Maksimović, J., Maksimović, V., Bonales-Alatorre, E., Shabala, L., Yu, M., Zhang, G.,& Živanović, Branka  D.. (2018). Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi. in Plant Cell and Environment
Wiley, Hoboken., 41(11), 2654-2667.
https://doi.org/10.1111/pce.13391

Zeng F, Shabala S, Dragišić Maksimović J, Maksimović V, Bonales-Alatorre E, Shabala L, Yu M, Zhang G, Živanović, Branka  D.. Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi. in Plant Cell and Environment. 2018;41(11):2654-2667.
doi:10.1111/pce.13391 .

Zeng, Fanrong, Shabala, Sergey, Dragišić Maksimović, Jelena, Maksimović, Vuk, Bonales-Alatorre, Edgar, Shabala, Lana, Yu, Min, Zhang, Guoping, Živanović, Branka  D., "Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi" in Plant Cell and Environment, 41, no. 11 (2018):2654-2667,
https://doi.org/10.1111/pce.13391 . .

TY  - JOUR
AU  - Ismail, Hebatollah
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Shabala, Lana
AU  - Živanović, Branka  D.
AU  - Tian, Yu
AU  - Jacobsen, Sven-Erik
AU  - Shabala, Sergey
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/971
AB  - The causal relationship between salinity and oxidative stress tolerance is well established, but specific downstream targets and the role of specific antioxidant compounds in controlling cellular ionic homeostasis remains elusive. In this work, we have compared antioxidant profiles of leaves of two quinoa genotypes contrasting in their salt tolerance, with the aim of understanding the role of enzymatic and non-enzymatic antioxidants in salinity stress tolerance. Only changes in superoxide dismutase activity were correlated with plant adaptive responses to salinity. Proline accumulation played no major role in either osmotic adjustment or in the tissue tolerance mechanism. Among other nonenzymatic antioxidants, rutin levels were increased by over 25 fold in quinoa leaves. Exogenous application of rutin to glycophyte bean leaves improved tissue tolerance and reduced detrimental effects of salinity on leaf photochemistry. Electrophysiological experiments revealed that these beneficial effects were attributed to improved potassium retention and increased rate of Na+ pumping from the cell. The lack of correlation between rutin-induced changes in K+ and H+ fluxes suggest that rutin accumulation in the cytosol scavenges hydroxyl radical formed in response to salinity treatment thus preventing K+ leak via one of ROS-activated K+ efflux pathways, rather than controlling K+ flux via voltage-gated K+-permeable channels.
PB  - Csiro Publishing, Clayton
T2  - Functional Plant Biology
T1  - Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans
EP  - 86
IS  - 1
SP  - 75
VL  - 43
DO  - 10.1071/FP15312
ER  - 

@article{
author = "Ismail, Hebatollah and Dragišić Maksimović, Jelena and Maksimović, Vuk and Shabala, Lana and Živanović, Branka  D. and Tian, Yu and Jacobsen, Sven-Erik and Shabala, Sergey",
year = "2016",
abstract = "The causal relationship between salinity and oxidative stress tolerance is well established, but specific downstream targets and the role of specific antioxidant compounds in controlling cellular ionic homeostasis remains elusive. In this work, we have compared antioxidant profiles of leaves of two quinoa genotypes contrasting in their salt tolerance, with the aim of understanding the role of enzymatic and non-enzymatic antioxidants in salinity stress tolerance. Only changes in superoxide dismutase activity were correlated with plant adaptive responses to salinity. Proline accumulation played no major role in either osmotic adjustment or in the tissue tolerance mechanism. Among other nonenzymatic antioxidants, rutin levels were increased by over 25 fold in quinoa leaves. Exogenous application of rutin to glycophyte bean leaves improved tissue tolerance and reduced detrimental effects of salinity on leaf photochemistry. Electrophysiological experiments revealed that these beneficial effects were attributed to improved potassium retention and increased rate of Na+ pumping from the cell. The lack of correlation between rutin-induced changes in K+ and H+ fluxes suggest that rutin accumulation in the cytosol scavenges hydroxyl radical formed in response to salinity treatment thus preventing K+ leak via one of ROS-activated K+ efflux pathways, rather than controlling K+ flux via voltage-gated K+-permeable channels.",
publisher = "Csiro Publishing, Clayton",
journal = "Functional Plant Biology",
title = "Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans",
pages = "86-75",
number = "1",
volume = "43",
doi = "10.1071/FP15312"
}

Ismail, H., Dragišić Maksimović, J., Maksimović, V., Shabala, L., Živanović, Branka  D., Tian, Y., Jacobsen, S.,& Shabala, S.. (2016). Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans. in Functional Plant Biology
Csiro Publishing, Clayton., 43(1), 75-86.
https://doi.org/10.1071/FP15312

Ismail H, Dragišić Maksimović J, Maksimović V, Shabala L, Živanović, Branka  D., Tian Y, Jacobsen S, Shabala S. Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans. in Functional Plant Biology. 2016;43(1):75-86.
doi:10.1071/FP15312 .

Ismail, Hebatollah, Dragišić Maksimović, Jelena, Maksimović, Vuk, Shabala, Lana, Živanović, Branka  D., Tian, Yu, Jacobsen, Sven-Erik, Shabala, Sergey, "Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans" in Functional Plant Biology, 43, no. 1 (2016):75-86,
https://doi.org/10.1071/FP15312 . .

TY  - JOUR
AU  - Živanović, Branka  D.
AU  - Shabala, Lana
AU  - Elzenga, Theo J. M.
AU  - Shabala, Sergey
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/867
AB  - Blue light signalling pathway in broad bean leaf epidermal cells includes key membrane transporters: plasma- and endomembrane channels and pumps of H (+) , Ca (2+) and K (+) ions, and plasma membrane redox system. Blue light signalling pathway in epidermal tissue isolated from the abaxial side of fully developed Vicia faba leaves was dissected by measuring the effect of inhibitors of second messengers on net K+, Ca2+ and H+ fluxes using non-invasive ion-selective microelectrodes (the MIFE system). Switching the blue light on-off caused transient changes of the ion fluxes. The effects of seven groups of inhibitors were tested in this study: CaM antagonists, ATPase inhibitors, Ca2+ anatagonists or chelators, agents affecting IP3 formation, redox system inhibitors, inhibitors of endomembrane Ca2+ transport systems and an inhibitor of plasma membrane Ca2+-permeable channels. Most of the inhibitors had a significant effect on steady-state (basal) net fluxes, as well as on the magnitude of the transient ion flux responses to blue light fluctuations. The data presented in this study suggest that redox signalling and, specifically, plasma membrane NADPH oxidase and coupled Ca2+ and K+ fluxes play an essential role in blue light signal transduction.
PB  - Springer, New York
T2  - Planta
T1  - Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach
EP  - 827
IS  - 4
SP  - 813
VL  - 242
DO  - 10.1007/s00425-015-2316-2
ER  - 

@article{
author = "Živanović, Branka  D. and Shabala, Lana and Elzenga, Theo J. M. and Shabala, Sergey",
year = "2015",
abstract = "Blue light signalling pathway in broad bean leaf epidermal cells includes key membrane transporters: plasma- and endomembrane channels and pumps of H (+) , Ca (2+) and K (+) ions, and plasma membrane redox system. Blue light signalling pathway in epidermal tissue isolated from the abaxial side of fully developed Vicia faba leaves was dissected by measuring the effect of inhibitors of second messengers on net K+, Ca2+ and H+ fluxes using non-invasive ion-selective microelectrodes (the MIFE system). Switching the blue light on-off caused transient changes of the ion fluxes. The effects of seven groups of inhibitors were tested in this study: CaM antagonists, ATPase inhibitors, Ca2+ anatagonists or chelators, agents affecting IP3 formation, redox system inhibitors, inhibitors of endomembrane Ca2+ transport systems and an inhibitor of plasma membrane Ca2+-permeable channels. Most of the inhibitors had a significant effect on steady-state (basal) net fluxes, as well as on the magnitude of the transient ion flux responses to blue light fluctuations. The data presented in this study suggest that redox signalling and, specifically, plasma membrane NADPH oxidase and coupled Ca2+ and K+ fluxes play an essential role in blue light signal transduction.",
publisher = "Springer, New York",
journal = "Planta",
title = "Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach",
pages = "827-813",
number = "4",
volume = "242",
doi = "10.1007/s00425-015-2316-2"
}

Živanović, Branka  D., Shabala, L., Elzenga, T. J. M.,& Shabala, S.. (2015). Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach. in Planta
Springer, New York., 242(4), 813-827.
https://doi.org/10.1007/s00425-015-2316-2

Živanović, Branka  D., Shabala L, Elzenga TJM, Shabala S. Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach. in Planta. 2015;242(4):813-827.
doi:10.1007/s00425-015-2316-2 .

Živanović, Branka  D., Shabala, Lana, Elzenga, Theo J. M., Shabala, Sergey, "Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach" in Planta, 242, no. 4 (2015):813-827,
https://doi.org/10.1007/s00425-015-2316-2 . .

TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Zhang, Jingyi
AU  - Zeng, Fanrong
AU  - Živanović, Branka  D.
AU  - Shabala, Lana
AU  - Zhou, Meixue
AU  - Shabala, Sergey
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/672
AB  - A causal relationship between salinity and oxidative stress tolerance and a suitability of using root antioxidant activity as a biochemical marker for salinity tolerance in barley was investigated. Net ion fluxes were measured from the mature zone of excised roots of two barley varieties contrasting in their salinity tolerance using non-invasive MIFE technique in response to acute and prolonged salinity treatment. These changes were correlated with activity of major antioxidant enzymes; ascorbate peroxidase, catalase, and superoxide dismutase. It was found that genotypic difference in salinity tolerance was largely independent of root integrity, and observed not only for short-term but also long-term NaCl exposures. Higher K+ retention ability (and, hence, salinity tolerance) positively correlated with oxidative stress tolerance. At the same time, antioxidant activities were constitutively higher in a sensitive but not tolerant variety, and no correlation was found between SOD activity and salinity tolerance index during large-scale screening. Although salinity tolerance in barley correlates with its oxidative stress tolerance, higher antioxidant activity at one particular time does not correlate with salinity tolerance and, as such, cannot be used as a biochemical marker in barley screening programs.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Linking oxidative and salinity stress tolerance in barley: can root antioxidant enzyme activity be used as a measure of stress tolerance?
EP  - 155
IS  - 1-2
SP  - 141
VL  - 365
DO  - 10.1007/s11104-012-1366-5
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Zhang, Jingyi and Zeng, Fanrong and Živanović, Branka  D. and Shabala, Lana and Zhou, Meixue and Shabala, Sergey",
year = "2013",
abstract = "A causal relationship between salinity and oxidative stress tolerance and a suitability of using root antioxidant activity as a biochemical marker for salinity tolerance in barley was investigated. Net ion fluxes were measured from the mature zone of excised roots of two barley varieties contrasting in their salinity tolerance using non-invasive MIFE technique in response to acute and prolonged salinity treatment. These changes were correlated with activity of major antioxidant enzymes; ascorbate peroxidase, catalase, and superoxide dismutase. It was found that genotypic difference in salinity tolerance was largely independent of root integrity, and observed not only for short-term but also long-term NaCl exposures. Higher K+ retention ability (and, hence, salinity tolerance) positively correlated with oxidative stress tolerance. At the same time, antioxidant activities were constitutively higher in a sensitive but not tolerant variety, and no correlation was found between SOD activity and salinity tolerance index during large-scale screening. Although salinity tolerance in barley correlates with its oxidative stress tolerance, higher antioxidant activity at one particular time does not correlate with salinity tolerance and, as such, cannot be used as a biochemical marker in barley screening programs.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Linking oxidative and salinity stress tolerance in barley: can root antioxidant enzyme activity be used as a measure of stress tolerance?",
pages = "155-141",
number = "1-2",
volume = "365",
doi = "10.1007/s11104-012-1366-5"
}

Dragišić Maksimović, J., Zhang, J., Zeng, F., Živanović, Branka  D., Shabala, L., Zhou, M.,& Shabala, S.. (2013). Linking oxidative and salinity stress tolerance in barley: can root antioxidant enzyme activity be used as a measure of stress tolerance?. in Plant and Soil
Springer, Dordrecht., 365(1-2), 141-155.
https://doi.org/10.1007/s11104-012-1366-5

Dragišić Maksimović J, Zhang J, Zeng F, Živanović, Branka  D., Shabala L, Zhou M, Shabala S. Linking oxidative and salinity stress tolerance in barley: can root antioxidant enzyme activity be used as a measure of stress tolerance?. in Plant and Soil. 2013;365(1-2):141-155.
doi:10.1007/s11104-012-1366-5 .

Dragišić Maksimović, Jelena, Zhang, Jingyi, Zeng, Fanrong, Živanović, Branka  D., Shabala, Lana, Zhou, Meixue, Shabala, Sergey, "Linking oxidative and salinity stress tolerance in barley: can root antioxidant enzyme activity be used as a measure of stress tolerance?" in Plant and Soil, 365, no. 1-2 (2013):141-155,
https://doi.org/10.1007/s11104-012-1366-5 . .

TY  - JOUR
AU  - Živanović, Branka  D.
AU  - Cuin, Tracey Ann
AU  - Shabala, Sergey
PY  - 2007
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/232
AB  - Two types of segments (intact leaf tissue and isolated mesophyll tissue respectively) were isolated from basal (still growing) and tip (non-growing) maize leaf regions. The leaf segments were exposed to different light qualities (blue or red light) and quantities, and net fluxes of K+, Ca2+ and H+ were measured non-invasively using ion-selective vibrating microelectrodes (the MIFF technique). A clear dose dependency of all ion flux responses on both red (RL) and blue (BL) light fluence rate was found. We provide evidence that light-induced K+ flux kinetics are different between growing and non-growing tissues and attribute this difference to the direct involvement of RL-induced K+ flux in turgor-driven leaf expansion growth controlled by the epidermis, as well as to the charge-balancing role of K+ in the leaf mesophyll. Generally, BL was much more efficient in stimulating K+ uptake in the growing basal region compared with RL. We also show a much stronger influence of RL on Ca2+ fluxes in the basal region compared with BL, which argues in favor of the importance of RL in Ca2+ signaling during leaf growth.
PB  - Oxford Univ Press, Oxford
T2  - Plant and Cell Physiology
T1  - Spectral and dose dependence of light-induced ion flux responses from maize leaves and their involvement in leaf expansion growth
EP  - 605
IS  - 4
SP  - 598
VL  - 48
DO  - 10.1093/pcp/pcm032
ER  - 

@article{
author = "Živanović, Branka  D. and Cuin, Tracey Ann and Shabala, Sergey",
year = "2007",
abstract = "Two types of segments (intact leaf tissue and isolated mesophyll tissue respectively) were isolated from basal (still growing) and tip (non-growing) maize leaf regions. The leaf segments were exposed to different light qualities (blue or red light) and quantities, and net fluxes of K+, Ca2+ and H+ were measured non-invasively using ion-selective vibrating microelectrodes (the MIFF technique). A clear dose dependency of all ion flux responses on both red (RL) and blue (BL) light fluence rate was found. We provide evidence that light-induced K+ flux kinetics are different between growing and non-growing tissues and attribute this difference to the direct involvement of RL-induced K+ flux in turgor-driven leaf expansion growth controlled by the epidermis, as well as to the charge-balancing role of K+ in the leaf mesophyll. Generally, BL was much more efficient in stimulating K+ uptake in the growing basal region compared with RL. We also show a much stronger influence of RL on Ca2+ fluxes in the basal region compared with BL, which argues in favor of the importance of RL in Ca2+ signaling during leaf growth.",
publisher = "Oxford Univ Press, Oxford",
journal = "Plant and Cell Physiology",
title = "Spectral and dose dependence of light-induced ion flux responses from maize leaves and their involvement in leaf expansion growth",
pages = "605-598",
number = "4",
volume = "48",
doi = "10.1093/pcp/pcm032"
}

Živanović, Branka  D., Cuin, T. A.,& Shabala, S.. (2007). Spectral and dose dependence of light-induced ion flux responses from maize leaves and their involvement in leaf expansion growth. in Plant and Cell Physiology
Oxford Univ Press, Oxford., 48(4), 598-605.
https://doi.org/10.1093/pcp/pcm032

Živanović, Branka  D., Cuin TA, Shabala S. Spectral and dose dependence of light-induced ion flux responses from maize leaves and their involvement in leaf expansion growth. in Plant and Cell Physiology. 2007;48(4):598-605.
doi:10.1093/pcp/pcm032 .

Živanović, Branka  D., Cuin, Tracey Ann, Shabala, Sergey, "Spectral and dose dependence of light-induced ion flux responses from maize leaves and their involvement in leaf expansion growth" in Plant and Cell Physiology, 48, no. 4 (2007):598-605,
https://doi.org/10.1093/pcp/pcm032 . .

TY  - JOUR
AU  - Živanović, Branka  D.
AU  - Pang, J
AU  - Shabala, Sergey
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/129
AB  - Net fluxes of H+, K+ and Ca2+ ions from maize (Zea mays L.) isolated leaf segments were measured non-invasively using ion-selective vibrating microelectrodes (the MIFE technique). Leaf segments were isolated from the blade base, containing actively elongating cells (basal segments), and from non-growing tip regions (tip segments). Ion fluxes were measured in response to bright white light (2600 mumol m(-2) s(-1)) from either the leaf segments or the underlying mesophyll (after stripping the epidermis). Fluxes measured from the mesophyll showed no significant difference between basal and tip regions. In leaf segments (epidermis attached), light-induced flux kinetics of all ions measured (H+, Ca2+ and K+) were strikingly different between the two regions. It appears that epidermal K+ fluxes are required to drive leaf expansion growth, whereas in the mesophyll light-induced K+ flux changes are likely to play a charge balancing role. Light-stimulated Ca2+ influx was not directly attributable either to leaf photosynthetic performance or to leaf expansion growth. It is concluded that light-induced ion flux changes are associated with both leaf growth and photosynthesis.
PB  - Wiley, Hoboken
T2  - Plant Cell and Environment
T1  - Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis
EP  - 352
IS  - 3
SP  - 340
VL  - 28
DO  - 10.1111/j.1365-3040.2005.01270.x
ER  - 

@article{
author = "Živanović, Branka  D. and Pang, J and Shabala, Sergey",
year = "2005",
abstract = "Net fluxes of H+, K+ and Ca2+ ions from maize (Zea mays L.) isolated leaf segments were measured non-invasively using ion-selective vibrating microelectrodes (the MIFE technique). Leaf segments were isolated from the blade base, containing actively elongating cells (basal segments), and from non-growing tip regions (tip segments). Ion fluxes were measured in response to bright white light (2600 mumol m(-2) s(-1)) from either the leaf segments or the underlying mesophyll (after stripping the epidermis). Fluxes measured from the mesophyll showed no significant difference between basal and tip regions. In leaf segments (epidermis attached), light-induced flux kinetics of all ions measured (H+, Ca2+ and K+) were strikingly different between the two regions. It appears that epidermal K+ fluxes are required to drive leaf expansion growth, whereas in the mesophyll light-induced K+ flux changes are likely to play a charge balancing role. Light-stimulated Ca2+ influx was not directly attributable either to leaf photosynthetic performance or to leaf expansion growth. It is concluded that light-induced ion flux changes are associated with both leaf growth and photosynthesis.",
publisher = "Wiley, Hoboken",
journal = "Plant Cell and Environment",
title = "Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis",
pages = "352-340",
number = "3",
volume = "28",
doi = "10.1111/j.1365-3040.2005.01270.x"
}

Živanović, Branka  D., Pang, J.,& Shabala, S.. (2005). Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis. in Plant Cell and Environment
Wiley, Hoboken., 28(3), 340-352.
https://doi.org/10.1111/j.1365-3040.2005.01270.x

Živanović, Branka  D., Pang J, Shabala S. Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis. in Plant Cell and Environment. 2005;28(3):340-352.
doi:10.1111/j.1365-3040.2005.01270.x .

Živanović, Branka  D., Pang, J, Shabala, Sergey, "Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis" in Plant Cell and Environment, 28, no. 3 (2005):340-352,
https://doi.org/10.1111/j.1365-3040.2005.01270.x . .