TY  - JOUR
AU  - Opačić, Miloš
AU  - Stević, Zorica D
AU  - Baščarević, Vladimir
AU  - Zivić, Miroslav
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1115
AB  - The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 00, 000-000.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Antioxidants & Redox Signaling
T1  - Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?
EP  - 1575
IS  - 17
SP  - 1570
VL  - 28
DO  - 10.1089/ars.2017.7433
ER  - 

@article{
author = "Opačić, Miloš and Stević, Zorica D and Baščarević, Vladimir and Zivić, Miroslav and Spasić, Mihajlo and Spasojević, Ivan",
year = "2018",
abstract = "The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 00, 000-000.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Antioxidants & Redox Signaling",
title = "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?",
pages = "1575-1570",
number = "17",
volume = "28",
doi = "10.1089/ars.2017.7433"
}

Opačić, M., Stević, Z. D., Baščarević, V., Zivić, M., Spasić, M.,& Spasojević, I.. (2018). Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants & Redox Signaling
Mary Ann Liebert, Inc, New Rochelle., 28(17), 1570-1575.
https://doi.org/10.1089/ars.2017.7433

Opačić M, Stević ZD, Baščarević V, Zivić M, Spasić M, Spasojević I. Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants & Redox Signaling. 2018;28(17):1570-1575.
doi:10.1089/ars.2017.7433 .

Opačić, Miloš, Stević, Zorica D, Baščarević, Vladimir, Zivić, Miroslav, Spasić, Mihajlo, Spasojević, Ivan, "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?" in Antioxidants & Redox Signaling, 28, no. 17 (2018):1570-1575,
https://doi.org/10.1089/ars.2017.7433 . .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstic, Miodrag
AU  - Spasic, Snežana
AU  - Miletic, Srdjan
AU  - Stefanovic-Kojic, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1050
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.
PB  - Elsevier Science Bv, Amsterdam
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
EP  - 136
SP  - 132
VL  - 100
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstic, Miodrag and Spasic, Snežana and Miletic, Srdjan and Stefanovic-Kojic, Jovana and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
pages = "136-132",
volume = "100",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstic, M., Spasic, S., Miletic, S., Stefanovic-Kojic, J., Nikolić-Kokić, A., Blagojević, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International
Elsevier Science Bv, Amsterdam., 100, 132-136.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstic M, Spasic S, Miletic S, Stefanovic-Kojic J, Nikolić-Kokić A, Blagojević D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;100:132-136.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstic, Miodrag, Spasic, Snežana, Miletic, Srdjan, Stefanovic-Kojic, Jovana, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International, 100 (2017):132-136,
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Spasojević, Ivan
AU  - Blagojević, Duško
AU  - Stević, Zorica D
AU  - Andjus, Pavle R.
AU  - Spasić, Mihajlo
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/902
AB  - In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The effects of wild-type and mutant sod1 on smooth muscle contraction
EP  - 192
IS  - 1
SP  - 187
VL  - 67
DO  - 10.2298/ABS141006023N
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Spasojević, Ivan and Blagojević, Duško and Stević, Zorica D and Andjus, Pavle R. and Spasić, Mihajlo",
year = "2015",
abstract = "In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The effects of wild-type and mutant sod1 on smooth muscle contraction",
pages = "192-187",
number = "1",
volume = "67",
doi = "10.2298/ABS141006023N"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Spasojević, I., Blagojević, D., Stević, Z. D., Andjus, P. R.,& Spasić, M.. (2015). The effects of wild-type and mutant sod1 on smooth muscle contraction. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(1), 187-192.
https://doi.org/10.2298/ABS141006023N

Nikolić-Kokić A, Oreščanin-Dušić Z, Spasojević I, Blagojević D, Stević ZD, Andjus PR, Spasić M. The effects of wild-type and mutant sod1 on smooth muscle contraction. in Archives of Biological Sciences. 2015;67(1):187-192.
doi:10.2298/ABS141006023N .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Spasojević, Ivan, Blagojević, Duško, Stević, Zorica D, Andjus, Pavle R., Spasić, Mihajlo, "The effects of wild-type and mutant sod1 on smooth muscle contraction" in Archives of Biological Sciences, 67, no. 1 (2015):187-192,
https://doi.org/10.2298/ABS141006023N . .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Bogdanović Pristov, Jelena
AU  - Mutavdžić, Dragosav
AU  - Savic, Aleksandar
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1875
AB  - Iron is essential for plant growth, metabolism of mitochondria and chloroplasts, and a wide assortment of enzymes. However, it is also involved in Fenton reaction that generates hydroxyl radical (HO•), the most reactive species in plants. The aim of our study was to examine the effects of amino acids on HO• production. The rank order according to antioxidative activity (AA) of amino acids in Fenton system was: Trp > Phe, Leu > Ile > His > Arg > Val > Lys, Tyr, Pro > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp. Sulfur-containing amino acids generated different secondary reactive products, which were discriminated by EPR spin-trapping spectroscopy. AA showed positive correlation with hydrophobicity and negative correlation with polarity. HO•provoked oxidation of amino acids was strongly positively affected by hydrophobic hydration. Group contribution method showed that the reactivity of amino acids with HO• is defined by the properties of side-chains (the contribution of NH3+CHCOO− group and antioxidative activity of Gly were next to zero). Our results might shed a new light on the role of iron in the regulation of free amino acids pool and on protein oxidation in plants. According to our findings, native proteins (with exposed polar residues) might not be particularly susceptible to oxidation by HO•. But once a protein is damaged and partially denatured it exposes hydrophobic side-chains and might become a likely target for HO•, thus potentially acquiring an antioxidative role. Further research on change of redox properties of proteins with denaturation is warranted.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
C3  - 2nd International Conference on Plant Biology • 21st Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015
T1  - The relationship of physicochemical properties and structure to the antioxidative activity of free amino acids in the aqueous Fenton system
SP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1875
ER  - 

@conference{
author = "Milić Komić, Sonja and Bogdanović Pristov, Jelena and Mutavdžić, Dragosav and Savic, Aleksandar and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Iron is essential for plant growth, metabolism of mitochondria and chloroplasts, and a wide assortment of enzymes. However, it is also involved in Fenton reaction that generates hydroxyl radical (HO•), the most reactive species in plants. The aim of our study was to examine the effects of amino acids on HO• production. The rank order according to antioxidative activity (AA) of amino acids in Fenton system was: Trp > Phe, Leu > Ile > His > Arg > Val > Lys, Tyr, Pro > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp. Sulfur-containing amino acids generated different secondary reactive products, which were discriminated by EPR spin-trapping spectroscopy. AA showed positive correlation with hydrophobicity and negative correlation with polarity. HO•provoked oxidation of amino acids was strongly positively affected by hydrophobic hydration. Group contribution method showed that the reactivity of amino acids with HO• is defined by the properties of side-chains (the contribution of NH3+CHCOO− group and antioxidative activity of Gly were next to zero). Our results might shed a new light on the role of iron in the regulation of free amino acids pool and on protein oxidation in plants. According to our findings, native proteins (with exposed polar residues) might not be particularly susceptible to oxidation by HO•. But once a protein is damaged and partially denatured it exposes hydrophobic side-chains and might become a likely target for HO•, thus potentially acquiring an antioxidative role. Further research on change of redox properties of proteins with denaturation is warranted.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade",
journal = "2nd International Conference on Plant Biology • 21st Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015",
title = "The relationship of physicochemical properties and structure to the antioxidative activity of free amino acids in the aqueous Fenton system",
pages = "106",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1875"
}

Milić Komić, S., Bogdanović Pristov, J., Mutavdžić, D., Savic, A., Spasić, M.,& Spasojević, I.. (2015). The relationship of physicochemical properties and structure to the antioxidative activity of free amino acids in the aqueous Fenton system. in 2nd International Conference on Plant Biology • 21st Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015
Serbian Plant Physiology Society., 106.
https://hdl.handle.net/21.15107/rcub_rimsi_1875

Milić Komić S, Bogdanović Pristov J, Mutavdžić D, Savic A, Spasić M, Spasojević I. The relationship of physicochemical properties and structure to the antioxidative activity of free amino acids in the aqueous Fenton system. in 2nd International Conference on Plant Biology • 21st Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015. 2015;:106.
https://hdl.handle.net/21.15107/rcub_rimsi_1875 .

Milić Komić, Sonja, Bogdanović Pristov, Jelena, Mutavdžić, Dragosav, Savic, Aleksandar, Spasić, Mihajlo, Spasojević, Ivan, "The relationship of physicochemical properties and structure to the antioxidative activity of free amino acids in the aqueous Fenton system" in 2nd International Conference on Plant Biology • 21st Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015 (2015):106,
https://hdl.handle.net/21.15107/rcub_rimsi_1875 .

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Bogdanović Pristov, Jelena
AU  - Mutavdžić, Dragosav
AU  - Savić, Aleksandar G
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/853
AB  - Herein we compared antioxidative activities (AA) of 25 free L-amino acids (FAA) against Fenton system-mediated hydroxyl radical (HO center dot) production in aqueous solution, and examined the relation between AA and a set of physicochemical properties. The tank order according -to AA was: Tip > notleucine > Phe, Lett > Ile > His >3,4-dihydrOxyphenylalanine, Arg > Val > Lys, Tyr, Pro > hydtoxyproline > alpha-aminobutyric acid > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp. Sulfur-containing FAA generated different secondary reactive products, which were discriminated by the means of electron paramagnetic resonance spin-trapping spectroscopy. AA showed a general positive correlation with hydrophobicity. However, when taken separately, uncharged FAA exhibited strong positive correlation of AA with hydrophobicity whereas charged FAA showed negative or no significant correlation depending on the scale applied. A general strong negative correlation was found between AA and polarity. Steric parameters and hydration numbers correlated positively with AA of rtoripolar side-chain FAA. In addition) a decrease of temperature which promotes hydrophobic hydration resulted in increased AA. This implies that HO-provoked oxidation of FAA is strongly affected by hydrophobic hydration. Our findings are important for the understanding of oxidation processes in natural and waste waters.
PB  - Amer Chemical Soc, Washington
T2  - Environmental Science & Technology
T1  - The Relationship of Physicochemical Properties to the Antioxidative Activity of Free Amino Acids in Fenton System
EP  - 4254
IS  - 7
SP  - 4245
VL  - 49
DO  - 10.1021/es5053396
ER  - 

@article{
author = "Milić Komić, Sonja and Bogdanović Pristov, Jelena and Mutavdžić, Dragosav and Savić, Aleksandar G and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Herein we compared antioxidative activities (AA) of 25 free L-amino acids (FAA) against Fenton system-mediated hydroxyl radical (HO center dot) production in aqueous solution, and examined the relation between AA and a set of physicochemical properties. The tank order according -to AA was: Tip > notleucine > Phe, Lett > Ile > His >3,4-dihydrOxyphenylalanine, Arg > Val > Lys, Tyr, Pro > hydtoxyproline > alpha-aminobutyric acid > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp. Sulfur-containing FAA generated different secondary reactive products, which were discriminated by the means of electron paramagnetic resonance spin-trapping spectroscopy. AA showed a general positive correlation with hydrophobicity. However, when taken separately, uncharged FAA exhibited strong positive correlation of AA with hydrophobicity whereas charged FAA showed negative or no significant correlation depending on the scale applied. A general strong negative correlation was found between AA and polarity. Steric parameters and hydration numbers correlated positively with AA of rtoripolar side-chain FAA. In addition) a decrease of temperature which promotes hydrophobic hydration resulted in increased AA. This implies that HO-provoked oxidation of FAA is strongly affected by hydrophobic hydration. Our findings are important for the understanding of oxidation processes in natural and waste waters.",
publisher = "Amer Chemical Soc, Washington",
journal = "Environmental Science & Technology",
title = "The Relationship of Physicochemical Properties to the Antioxidative Activity of Free Amino Acids in Fenton System",
pages = "4254-4245",
number = "7",
volume = "49",
doi = "10.1021/es5053396"
}

Milić Komić, S., Bogdanović Pristov, J., Mutavdžić, D., Savić, A. G., Spasić, M.,& Spasojević, I.. (2015). The Relationship of Physicochemical Properties to the Antioxidative Activity of Free Amino Acids in Fenton System. in Environmental Science & Technology
Amer Chemical Soc, Washington., 49(7), 4245-4254.
https://doi.org/10.1021/es5053396

Milić Komić S, Bogdanović Pristov J, Mutavdžić D, Savić AG, Spasić M, Spasojević I. The Relationship of Physicochemical Properties to the Antioxidative Activity of Free Amino Acids in Fenton System. in Environmental Science & Technology. 2015;49(7):4245-4254.
doi:10.1021/es5053396 .

Milić Komić, Sonja, Bogdanović Pristov, Jelena, Mutavdžić, Dragosav, Savić, Aleksandar G, Spasić, Mihajlo, Spasojević, Ivan, "The Relationship of Physicochemical Properties to the Antioxidative Activity of Free Amino Acids in Fenton System" in Environmental Science & Technology, 49, no. 7 (2015):4245-4254,
https://doi.org/10.1021/es5053396 . .

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijusković, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasic, Snežana
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/846
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
EP  - 23
SP  - 19
VL  - 51
DO  - 10.1016/j.niox.2015.09.008
ER  - 

@article{
author = "Bolic, Bojana and Mijusković, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasic, Snežana and Blagojević, Duško and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study",
pages = "23-19",
volume = "51",
doi = "10.1016/j.niox.2015.09.008"
}

Bolic, B., Mijusković, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasic, S., Blagojević, D., Spasić, M.,& Spasojević, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijusković A, Popovic-Bijelic A, Nikolić-Kokić A, Spasic S, Blagojević D, Spasić M, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijusković, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasic, Snežana, Blagojević, Duško, Spasić, Mihajlo, Spasojević, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Spasojević, Ivan
AU  - Slavić, Marija
AU  - Mijusković, Ana
AU  - Paskulin, Roman
AU  - Miljević, Cedo
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško P.
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/889
AB  - Ethnopharmacological relevance: Ibogaine is a naturally occurring alkaloid with psychotropic and metabotropic effects, derived from the bark of the root of the West African Tabernanthe iboga plant. The tribes of Kongo basin have been using iboga as a stimulant, for medicinal purposes, and in rite of passage ceremonies, for centuries. Besides, it has been found that this drug has anti-addictive effects. Aim of the study: Previous studies have demonstrated that ibogaine changed the quantity of ATP and energy related enzymes as well as the activity of antioxidant enzymes in cells thus altering redox equilibrium in a time manner. In this work, the mechanism of its action was further studied by measuring the effects of ibogaine in human erythrocytes in vitro on ATP liberation, membrane fluidity and antioxidant enzymes activity. Materials and methods: Heparinized human blood samples were incubated with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was separated by centrifugation and the levels of ATP and uric acid were measured 10 mm after the addition of ibogaine using standard kits. The activity of copper zinc superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were measured in erythrocytes after incubation period. The stability of SOD1 activity was further tested through in vitro incubation with H2O2 and scanning of its electrophoretic profiles. Membrane fluidity was determined using an electron paramagnetic resonance spin-labelling method. Results: Results showed that ibogaine treatment of erythrocytes in vitro increased ATP concentration in the blood plasma without changes in neither erythrocytes membrane fluidity nor uric acid concentration. lbogaine also increased SOD1 activity in erythrocytes at both doses applied here. Treatment with 20 mu M also elevated GR activity after in vitro incubation at 37 degrees C. Electrophoretic profiles revealed that incubation with ibogaine mitigates H2O2 mediated suppression of SOD1 activity. Conclusion: Some of the effects of ibogaine seem to be mediated through its influence on energy metabolism, redox active processes and the effects of discrete fluctuations of individual reactive oxygen species on different levels of enzyme activities. Overall, ibogaine acts as a pro-antioxidant by increasing activity of antioxidative enzymes and as an adaptagene in oxidative distress.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes
EP  - 70
SP  - 64
VL  - 164
DO  - 10.1016/j.jep.2015.01.037
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Spasojević, Ivan and Slavić, Marija and Mijusković, Ana and Paskulin, Roman and Miljević, Cedo and Spasić, Mihajlo and Blagojević, Duško P.",
year = "2015",
abstract = "Ethnopharmacological relevance: Ibogaine is a naturally occurring alkaloid with psychotropic and metabotropic effects, derived from the bark of the root of the West African Tabernanthe iboga plant. The tribes of Kongo basin have been using iboga as a stimulant, for medicinal purposes, and in rite of passage ceremonies, for centuries. Besides, it has been found that this drug has anti-addictive effects. Aim of the study: Previous studies have demonstrated that ibogaine changed the quantity of ATP and energy related enzymes as well as the activity of antioxidant enzymes in cells thus altering redox equilibrium in a time manner. In this work, the mechanism of its action was further studied by measuring the effects of ibogaine in human erythrocytes in vitro on ATP liberation, membrane fluidity and antioxidant enzymes activity. Materials and methods: Heparinized human blood samples were incubated with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was separated by centrifugation and the levels of ATP and uric acid were measured 10 mm after the addition of ibogaine using standard kits. The activity of copper zinc superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were measured in erythrocytes after incubation period. The stability of SOD1 activity was further tested through in vitro incubation with H2O2 and scanning of its electrophoretic profiles. Membrane fluidity was determined using an electron paramagnetic resonance spin-labelling method. Results: Results showed that ibogaine treatment of erythrocytes in vitro increased ATP concentration in the blood plasma without changes in neither erythrocytes membrane fluidity nor uric acid concentration. lbogaine also increased SOD1 activity in erythrocytes at both doses applied here. Treatment with 20 mu M also elevated GR activity after in vitro incubation at 37 degrees C. Electrophoretic profiles revealed that incubation with ibogaine mitigates H2O2 mediated suppression of SOD1 activity. Conclusion: Some of the effects of ibogaine seem to be mediated through its influence on energy metabolism, redox active processes and the effects of discrete fluctuations of individual reactive oxygen species on different levels of enzyme activities. Overall, ibogaine acts as a pro-antioxidant by increasing activity of antioxidative enzymes and as an adaptagene in oxidative distress.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes",
pages = "70-64",
volume = "164",
doi = "10.1016/j.jep.2015.01.037"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Spasojević, I., Slavić, M., Mijusković, A., Paskulin, R., Miljević, C., Spasić, M.,& Blagojević, D. P.. (2015). Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 164, 64-70.
https://doi.org/10.1016/j.jep.2015.01.037

Nikolić-Kokić A, Oreščanin-Dušić Z, Spasojević I, Slavić M, Mijusković A, Paskulin R, Miljević C, Spasić M, Blagojević DP. Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes. in Journal of Ethnopharmacology. 2015;164:64-70.
doi:10.1016/j.jep.2015.01.037 .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Spasojević, Ivan, Slavić, Marija, Mijusković, Ana, Paskulin, Roman, Miljević, Cedo, Spasić, Mihajlo, Blagojević, Duško P., "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes" in Journal of Ethnopharmacology, 164 (2015):64-70,
https://doi.org/10.1016/j.jep.2015.01.037 . .

TY  - GEN
AU  - Mijusković, Ana
AU  - Oreščanin-Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavić, Marija
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
AU  - Blagojević, Duško P
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/774
AB  - H2S was shown as an uterine relaxant. However, the signaling pathways, including ion channels regulated by H2S mediating relaxation in uterus are still unknown. The effects on contractility in response to sodium sulphide Na2S were examined on myometrial strips from virgin Wistar rats. Our results showed that Na2S induces concentration-dependent relaxations affecting amplitude as well as frequency of contractions. Activation of potassium channels, and in particular of KATP, was one of the primary mechanisms proposed, responsible for the relaxing effects of H2S. Sodium sulphide (20–200 × 10−6 M) inhibits myometrial contractility through a K-channel-independent mechanism. An inhibitor of 4,4 – inhibitor of Cl-/HCO3- exchanger and/or Cl− channel, DIDS, caused a significant rightward shift of the Na2S concentration–response curve. We performed experiments aimed at different Ca2+ concentrations, using spontaneous, calcium and KCl (15 mM and 75 mM) induced contractions, as well as pharmacological inhibitors of calcium channels and modulators, showing that Na2S induced relaxation is dependent on the precontractile agent used. Taken together, our results suggests that decreased frequency induced by Na2S could be a consequence of a alterated pacemaker cells which might be related for Ca2+ events originates from sarco endoplasmatic reticulum and/or mitochondria.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Sodium sulphide relaxation of rat uterus is related to calcium signaling
EP  - S37
SP  - S36
VL  - 39
DO  - 10.1016/j.niox.2014.03.119
ER  - 

@misc{
author = "Mijusković, Ana and Oreščanin-Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavić, Marija and Spasić, Mihajlo and Spasojević, Ivan and Blagojević, Duško P",
year = "2014",
abstract = "H2S was shown as an uterine relaxant. However, the signaling pathways, including ion channels regulated by H2S mediating relaxation in uterus are still unknown. The effects on contractility in response to sodium sulphide Na2S were examined on myometrial strips from virgin Wistar rats. Our results showed that Na2S induces concentration-dependent relaxations affecting amplitude as well as frequency of contractions. Activation of potassium channels, and in particular of KATP, was one of the primary mechanisms proposed, responsible for the relaxing effects of H2S. Sodium sulphide (20–200 × 10−6 M) inhibits myometrial contractility through a K-channel-independent mechanism. An inhibitor of 4,4 – inhibitor of Cl-/HCO3- exchanger and/or Cl− channel, DIDS, caused a significant rightward shift of the Na2S concentration–response curve. We performed experiments aimed at different Ca2+ concentrations, using spontaneous, calcium and KCl (15 mM and 75 mM) induced contractions, as well as pharmacological inhibitors of calcium channels and modulators, showing that Na2S induced relaxation is dependent on the precontractile agent used. Taken together, our results suggests that decreased frequency induced by Na2S could be a consequence of a alterated pacemaker cells which might be related for Ca2+ events originates from sarco endoplasmatic reticulum and/or mitochondria.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Sodium sulphide relaxation of rat uterus is related to calcium signaling",
pages = "S37-S36",
volume = "39",
doi = "10.1016/j.niox.2014.03.119"
}

Mijusković, A., Oreščanin-Dušić, Z., Nikolić-Kokić, A., Slavić, M., Spasić, M., Spasojević, I.,& Blagojević, D. P.. (2014). Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 39, S36-S37.
https://doi.org/10.1016/j.niox.2014.03.119

Mijusković A, Oreščanin-Dušić Z, Nikolić-Kokić A, Slavić M, Spasić M, Spasojević I, Blagojević DP. Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry. 2014;39:S36-S37.
doi:10.1016/j.niox.2014.03.119 .

Mijusković, Ana, Oreščanin-Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavić, Marija, Spasić, Mihajlo, Spasojević, Ivan, Blagojević, Duško P, "Sodium sulphide relaxation of rat uterus is related to calcium signaling" in Nitric Oxide-Biology and Chemistry, 39 (2014):S36-S37,
https://doi.org/10.1016/j.niox.2014.03.119 . .

TY  - JOUR
AU  - Mijusković, Ana
AU  - Oreščanin-Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavić, Marija
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
AU  - Blagojević, Duško P
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/787
AB  - Background: Our aim was to investigate the effect of methanethiol (CH3SH) on contractility of rat uterus and activities of redox-active enzymes, and to compare them with the effect of sodium sulphide (Na2S), a hydrogen sulphide (H2S/HS-) donor. Methods: Uteri were isolated from virgin Wistar rats, divided into six groups, controls (untreated uteri allowed to contract spontaneously and in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced). Underlying antioxidative enzyme activities (superoxide dismutase - SOD, glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or Na2S-treated uteri were compared to controls. Results: Our experiments showed that CH3SH and Na2S provoked reversible relaxation of both spontaneous and Ca2+ induced uterine contractions. The dose-response curves differed in shape, and CH3SH curve was shifted to higher concentration compared to H2S/HS-. The effects of Na2S fitted sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked increased SOD activity and decreased GR activity. However, Na2S (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+ stimulated uteri, while the activity of GSHPx was increased in both types of active uteri. Conclusion: Our results imply that CH3SH may have a constructive role in the control of muscle function and metabolism. Observed differences between CH3SH and H2S/HS- could be attributed to a larger moiety that is present in CH3SH compared to H2S, but they are more likely to be a consequence of the specific actions of HS-, in relation to its negative charge.
PB  - Polish Acad Sciences Inst Pharmacology, Krakow
T2  - Pharmacological Reports
T1  - Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity
EP  - 379
IS  - 3
SP  - 373
VL  - 66
DO  - 10.1016/j.pharep.2013.12.012
ER  - 

@article{
author = "Mijusković, Ana and Oreščanin-Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavić, Marija and Spasić, Mihajlo and Spasojević, Ivan and Blagojević, Duško P",
year = "2014",
abstract = "Background: Our aim was to investigate the effect of methanethiol (CH3SH) on contractility of rat uterus and activities of redox-active enzymes, and to compare them with the effect of sodium sulphide (Na2S), a hydrogen sulphide (H2S/HS-) donor. Methods: Uteri were isolated from virgin Wistar rats, divided into six groups, controls (untreated uteri allowed to contract spontaneously and in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced). Underlying antioxidative enzyme activities (superoxide dismutase - SOD, glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or Na2S-treated uteri were compared to controls. Results: Our experiments showed that CH3SH and Na2S provoked reversible relaxation of both spontaneous and Ca2+ induced uterine contractions. The dose-response curves differed in shape, and CH3SH curve was shifted to higher concentration compared to H2S/HS-. The effects of Na2S fitted sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked increased SOD activity and decreased GR activity. However, Na2S (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+ stimulated uteri, while the activity of GSHPx was increased in both types of active uteri. Conclusion: Our results imply that CH3SH may have a constructive role in the control of muscle function and metabolism. Observed differences between CH3SH and H2S/HS- could be attributed to a larger moiety that is present in CH3SH compared to H2S, but they are more likely to be a consequence of the specific actions of HS-, in relation to its negative charge.",
publisher = "Polish Acad Sciences Inst Pharmacology, Krakow",
journal = "Pharmacological Reports",
title = "Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity",
pages = "379-373",
number = "3",
volume = "66",
doi = "10.1016/j.pharep.2013.12.012"
}

Mijusković, A., Oreščanin-Dušić, Z., Nikolić-Kokić, A., Slavić, M., Spasić, M., Spasojević, I.,& Blagojević, D. P.. (2014). Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity. in Pharmacological Reports
Polish Acad Sciences Inst Pharmacology, Krakow., 66(3), 373-379.
https://doi.org/10.1016/j.pharep.2013.12.012

Mijusković A, Oreščanin-Dušić Z, Nikolić-Kokić A, Slavić M, Spasić M, Spasojević I, Blagojević DP. Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity. in Pharmacological Reports. 2014;66(3):373-379.
doi:10.1016/j.pharep.2013.12.012 .

Mijusković, Ana, Oreščanin-Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavić, Marija, Spasić, Mihajlo, Spasojević, Ivan, Blagojević, Duško P, "Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity" in Pharmacological Reports, 66, no. 3 (2014):373-379,
https://doi.org/10.1016/j.pharep.2013.12.012 . .

TY  - JOUR
AU  - Bajić, Aleksandar
AU  - Spasić, Mihajlo
AU  - Andjus, Pavle R.
AU  - Savić, Danijela Z
AU  - Parabucki, Ana B
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/707
AB  - The effects of H2O2 are widely studied in cell cultures and other in vitro systems. However, such investigations are performed with the assumption that H2O2 concentration is constant, which may not properly reflect in vivo settings, particularly in redox-turbulent microenvironments such as mitochondria. Here we introduced and tested a novel concept of fluctuating oxidative stress. We treated C6 astroglial cells and primary astrocytes with H2O2, using three regimes of exposure - continuous, as well as fluctuating at low or high rate, and evaluated mitochondrial membrane potential and other parameters of mitochondrial activity - respiration, reducing capacity, and superoxide production, as well as intracellular ATP, intracellular calcium, and NF-kappa B activation. When compared to continuous exposure, fluctuating H2O2 induced a pronounced hyperpolarization in mitochondria, whereas the activity of electron transport chain appears not to be significantly affected. H2O2 provoked a decrease of ATP level and an increase of intracellular calcium concentration, independently of the regime of treatment. However, fluctuating H2O2 induced a specific pattern of large-amplitude fluctuations of calcium concentration. An impact on NF kappa B activation was observed for high rate fluctuations, whereas continuous and low rate fluctuating oxidative stress did not provoke significant effects. Presented results outline the (patho)physiological relevance of redox fluctuations.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia
IS  - 10
VL  - 8
DO  - 10.1371/journal.pone.0076383
ER  - 

@article{
author = "Bajić, Aleksandar and Spasić, Mihajlo and Andjus, Pavle R. and Savić, Danijela Z and Parabucki, Ana B and Nikolić-Kokić, Aleksandra and Spasojević, Ivan",
year = "2013",
abstract = "The effects of H2O2 are widely studied in cell cultures and other in vitro systems. However, such investigations are performed with the assumption that H2O2 concentration is constant, which may not properly reflect in vivo settings, particularly in redox-turbulent microenvironments such as mitochondria. Here we introduced and tested a novel concept of fluctuating oxidative stress. We treated C6 astroglial cells and primary astrocytes with H2O2, using three regimes of exposure - continuous, as well as fluctuating at low or high rate, and evaluated mitochondrial membrane potential and other parameters of mitochondrial activity - respiration, reducing capacity, and superoxide production, as well as intracellular ATP, intracellular calcium, and NF-kappa B activation. When compared to continuous exposure, fluctuating H2O2 induced a pronounced hyperpolarization in mitochondria, whereas the activity of electron transport chain appears not to be significantly affected. H2O2 provoked a decrease of ATP level and an increase of intracellular calcium concentration, independently of the regime of treatment. However, fluctuating H2O2 induced a specific pattern of large-amplitude fluctuations of calcium concentration. An impact on NF kappa B activation was observed for high rate fluctuations, whereas continuous and low rate fluctuating oxidative stress did not provoke significant effects. Presented results outline the (patho)physiological relevance of redox fluctuations.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia",
number = "10",
volume = "8",
doi = "10.1371/journal.pone.0076383"
}

Bajić, A., Spasić, M., Andjus, P. R., Savić, D. Z., Parabucki, A. B., Nikolić-Kokić, A.,& Spasojević, I.. (2013). Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia. in PLoS One
Public Library Science, San Francisco., 8(10).
https://doi.org/10.1371/journal.pone.0076383

Bajić A, Spasić M, Andjus PR, Savić DZ, Parabucki AB, Nikolić-Kokić A, Spasojević I. Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia. in PLoS One. 2013;8(10).
doi:10.1371/journal.pone.0076383 .

Bajić, Aleksandar, Spasić, Mihajlo, Andjus, Pavle R., Savić, Danijela Z, Parabucki, Ana B, Nikolić-Kokić, Aleksandra, Spasojević, Ivan, "Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia" in PLoS One, 8, no. 10 (2013),
https://doi.org/10.1371/journal.pone.0076383 . .

TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/644
AB  - In contrast to the damaging effects of high doses, low dose radiation (UV, gamma) has been reported to provoke constructive changes in plants. However, the mechanisms by which plants recognize and respond to low dose radiation are not understood. We have shown recently that polygalacturonic acid, cell wall polysaccharide, converts the highly reactive product of radiation - hydroxyl radical into superoxide which may be further dismutated to hydrogen peroxide. Superoxide has been proposed to act as a signaling molecule, while hydrogen peroxide is known to be the key species in redox signaling cascades which are involved in the regulation of various physiological processes. Hence we propose that polygalacturonic acid may operate as radiation-signaling convertor. The outlined principles of radiation-sensing could also be valid for mammalian cells, with some other molecules mediating the conversion.
T2  - Plant Signaling and Behavior
T1  - Converting low dose radiation to redox signaling
IS  - 2
VL  - 8
DO  - 10.4161/psb.23151
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Spasić, Mihajlo and Spasojević, Ivan",
year = "2013",
abstract = "In contrast to the damaging effects of high doses, low dose radiation (UV, gamma) has been reported to provoke constructive changes in plants. However, the mechanisms by which plants recognize and respond to low dose radiation are not understood. We have shown recently that polygalacturonic acid, cell wall polysaccharide, converts the highly reactive product of radiation - hydroxyl radical into superoxide which may be further dismutated to hydrogen peroxide. Superoxide has been proposed to act as a signaling molecule, while hydrogen peroxide is known to be the key species in redox signaling cascades which are involved in the regulation of various physiological processes. Hence we propose that polygalacturonic acid may operate as radiation-signaling convertor. The outlined principles of radiation-sensing could also be valid for mammalian cells, with some other molecules mediating the conversion.",
journal = "Plant Signaling and Behavior",
title = "Converting low dose radiation to redox signaling",
number = "2",
volume = "8",
doi = "10.4161/psb.23151"
}

Bogdanović Pristov, J., Spasić, M.,& Spasojević, I.. (2013). Converting low dose radiation to redox signaling. in Plant Signaling and Behavior, 8(2).
https://doi.org/10.4161/psb.23151

Bogdanović Pristov J, Spasić M, Spasojević I. Converting low dose radiation to redox signaling. in Plant Signaling and Behavior. 2013;8(2).
doi:10.4161/psb.23151 .

Bogdanović Pristov, Jelena, Spasić, Mihajlo, Spasojević, Ivan, "Converting low dose radiation to redox signaling" in Plant Signaling and Behavior, 8, no. 2 (2013),
https://doi.org/10.4161/psb.23151 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Slavić, Marija
AU  - Spasojević, Ivan
AU  - Stević, Zorica D
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško P
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/680
AB  - Uvod: Mutirana bakar, cink superoksid-dizmutaza (SOD1) može da pravi agregate, sto predstavlja početni uzrok oštećenja motornog neurona može da izazove nastanak bolesti. U ovom radu su pokazani efekti humane bakar, cink super-oksid dizmutaze iz krvi pacijenata obolelih od familijarne amiotrofične lateralne skleroze (FALS) sa Leu144Phe (L144F) mutacijom i normalne (wild-type - WT) humane SOD1, iz krvi zdravih kontrola, na glatkom mišiću. Metode: Izolovali smo WT i L144F SOD1 enzime kod osam odabranih FALS pacijenata sa L144F mutacijom na egzonu 5 i pet zdravih kontrola. Dalje smo ispitivali aktivnost SOD1 u dobijenim uzorcima adrenalinskom metodom i elektro-foretski ih profilisali. Konačno, izolovanu WT i L144F SOD1 aplicirali smo na izolovani uterus pacova. Rezultati: Aktivnost L144F SOD1 je statistički značajno manja (p lt 0,05) u poređenju sa aktivnosti WT SOD1 zdravih kontrola. L144F ne izaziva relaksaciju glatkog mišića, kao sto je to slučaj sa WT SOD1. Zaključak: Naši rezultati pokazuju da izostanak relaksacije mišićnog tonusa u prisustvu mutirane SOD1 može imati štetni povratni efekat kod FALS pacijenata.
AB  - Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 protein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle. Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and profiled them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus. Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation. Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića
T1  - The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions
EP  - 379
IS  - 4
SP  - 375
VL  - 32
DO  - 10.2478/jomb-2013-0032
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Slavić, Marija and Spasojević, Ivan and Stević, Zorica D and Spasić, Mihajlo and Blagojević, Duško P",
year = "2013",
abstract = "Uvod: Mutirana bakar, cink superoksid-dizmutaza (SOD1) može da pravi agregate, sto predstavlja početni uzrok oštećenja motornog neurona može da izazove nastanak bolesti. U ovom radu su pokazani efekti humane bakar, cink super-oksid dizmutaze iz krvi pacijenata obolelih od familijarne amiotrofične lateralne skleroze (FALS) sa Leu144Phe (L144F) mutacijom i normalne (wild-type - WT) humane SOD1, iz krvi zdravih kontrola, na glatkom mišiću. Metode: Izolovali smo WT i L144F SOD1 enzime kod osam odabranih FALS pacijenata sa L144F mutacijom na egzonu 5 i pet zdravih kontrola. Dalje smo ispitivali aktivnost SOD1 u dobijenim uzorcima adrenalinskom metodom i elektro-foretski ih profilisali. Konačno, izolovanu WT i L144F SOD1 aplicirali smo na izolovani uterus pacova. Rezultati: Aktivnost L144F SOD1 je statistički značajno manja (p lt 0,05) u poređenju sa aktivnosti WT SOD1 zdravih kontrola. L144F ne izaziva relaksaciju glatkog mišića, kao sto je to slučaj sa WT SOD1. Zaključak: Naši rezultati pokazuju da izostanak relaksacije mišićnog tonusa u prisustvu mutirane SOD1 može imati štetni povratni efekat kod FALS pacijenata., Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 protein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle. Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and profiled them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus. Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation. Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića, The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions",
pages = "379-375",
number = "4",
volume = "32",
doi = "10.2478/jomb-2013-0032"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Slavić, M., Spasojević, I., Stević, Z. D., Spasić, M.,& Blagojević, D. P.. (2013). Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 32(4), 375-379.
https://doi.org/10.2478/jomb-2013-0032

Nikolić-Kokić A, Oreščanin-Dušić Z, Slavić M, Spasojević I, Stević ZD, Spasić M, Blagojević DP. Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića. in Journal of Medical Biochemistry. 2013;32(4):375-379.
doi:10.2478/jomb-2013-0032 .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Slavić, Marija, Spasojević, Ivan, Stević, Zorica D, Spasić, Mihajlo, Blagojević, Duško P, "Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića" in Journal of Medical Biochemistry, 32, no. 4 (2013):375-379,
https://doi.org/10.2478/jomb-2013-0032 . .

TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Maglic, Dragana
AU  - Opačić, Miloš
AU  - Mandic, Vesna
AU  - Miković, Zeljko
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/585
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Thrombosis Research
T1  - Ante- and postpartum redox status of blood in women with inherited thrombophilia treated with heparin
EP  - 829
IS  - 5
SP  - 826
VL  - 130
DO  - 10.1016/j.thromres.2012.08.310
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Maglic, Dragana and Opačić, Miloš and Mandic, Vesna and Miković, Zeljko and Spasić, Mihajlo and Spasojević, Ivan",
year = "2012",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Thrombosis Research",
title = "Ante- and postpartum redox status of blood in women with inherited thrombophilia treated with heparin",
pages = "829-826",
number = "5",
volume = "130",
doi = "10.1016/j.thromres.2012.08.310"
}

Bogdanović Pristov, J., Maglic, D., Opačić, M., Mandic, V., Miković, Z., Spasić, M.,& Spasojević, I.. (2012). Ante- and postpartum redox status of blood in women with inherited thrombophilia treated with heparin. in Thrombosis Research
Pergamon-Elsevier Science Ltd, Oxford., 130(5), 826-829.
https://doi.org/10.1016/j.thromres.2012.08.310

Bogdanović Pristov J, Maglic D, Opačić M, Mandic V, Miković Z, Spasić M, Spasojević I. Ante- and postpartum redox status of blood in women with inherited thrombophilia treated with heparin. in Thrombosis Research. 2012;130(5):826-829.
doi:10.1016/j.thromres.2012.08.310 .

Bogdanović Pristov, Jelena, Maglic, Dragana, Opačić, Miloš, Mandic, Vesna, Miković, Zeljko, Spasić, Mihajlo, Spasojević, Ivan, "Ante- and postpartum redox status of blood in women with inherited thrombophilia treated with heparin" in Thrombosis Research, 130, no. 5 (2012):826-829,
https://doi.org/10.1016/j.thromres.2012.08.310 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
AU  - Vujisic, Ljubodrag V
AU  - Spasić, Mihajlo
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/545
AB  - The mechanisms of reaction of methionine with hydroxyl radical are not fully understood. Here, we unequivocally show using electron paramagnetic resonance spin-trapping spectroscopy and GC-FID and GC-MS, the presence of specific carbon-, nitrogen- and sulfur-centered radicals as intermediates of this reaction, as well as the liberation of methanethiol as a gaseous end product. Taking into account the many roles that methionine has in eco- and biosystems, our results may elucidate redox chemistry of this amino acid and processes that methionine is involved in.
PB  - Springer Wien, Wien
T2  - Amino Acids
T1  - The reaction of methionine with hydroxyl radical: reactive intermediates and methanethiol production
EP  - 2445
IS  - 6
SP  - 2439
VL  - 42
DO  - 10.1007/s00726-011-1049-1
ER  - 

@article{
author = "Spasojević, Ivan and Bogdanović Pristov, Jelena and Vujisic, Ljubodrag V and Spasić, Mihajlo",
year = "2012",
abstract = "The mechanisms of reaction of methionine with hydroxyl radical are not fully understood. Here, we unequivocally show using electron paramagnetic resonance spin-trapping spectroscopy and GC-FID and GC-MS, the presence of specific carbon-, nitrogen- and sulfur-centered radicals as intermediates of this reaction, as well as the liberation of methanethiol as a gaseous end product. Taking into account the many roles that methionine has in eco- and biosystems, our results may elucidate redox chemistry of this amino acid and processes that methionine is involved in.",
publisher = "Springer Wien, Wien",
journal = "Amino Acids",
title = "The reaction of methionine with hydroxyl radical: reactive intermediates and methanethiol production",
pages = "2445-2439",
number = "6",
volume = "42",
doi = "10.1007/s00726-011-1049-1"
}

Spasojević, I., Bogdanović Pristov, J., Vujisic, L. V.,& Spasić, M.. (2012). The reaction of methionine with hydroxyl radical: reactive intermediates and methanethiol production. in Amino Acids
Springer Wien, Wien., 42(6), 2439-2445.
https://doi.org/10.1007/s00726-011-1049-1

Spasojević I, Bogdanović Pristov J, Vujisic LV, Spasić M. The reaction of methionine with hydroxyl radical: reactive intermediates and methanethiol production. in Amino Acids. 2012;42(6):2439-2445.
doi:10.1007/s00726-011-1049-1 .

Spasojević, Ivan, Bogdanović Pristov, Jelena, Vujisic, Ljubodrag V, Spasić, Mihajlo, "The reaction of methionine with hydroxyl radical: reactive intermediates and methanethiol production" in Amino Acids, 42, no. 6 (2012):2439-2445,
https://doi.org/10.1007/s00726-011-1049-1 . .

TY  - JOUR
AU  - Ignjatović, Aleksandar
AU  - Stević, Zorica D
AU  - Lavrnic, Dragana S
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško P
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/535
AB  - ALS is characterized by oxidative damage in the brain and cerebrospinal fluid, which is exerted by pro-oxidative activity of iron. Such activity of iron can be drastically increased in the presence of inappropriate iron ligands that catalyze redox cycling of iron, thereby promoting hydroxyl radical generation. The aim of our study was to determine the relative level of inappropriate iron ligands in the cerebrospinal fluid of ALS patients. To determine the levels of inappropriate iron ligands and redox activity of iron in cerebrospinal fluid (10 samples from ALS patients and 10 controls), we applied electron paramagnetic resonance spectroscopy. We have shown that cerebrospinal fluid of ALS patients comprises twofold increased level of inappropriate iron ligands, proportionally increasing iron redox activity and hydroxyl radical production compared to controls. In conclusion, our results strongly support the pro-oxidative/detrimental role of inappropriately chelated iron in ALS pathophysiology. The identification of biomolecules that form such iron complexes and their therapeutic targeting may represent the future of ALS treatment.
PB  - Informa Healthcare, London
T2  - Amyotrophic Lateral Sclerosis
T1  - Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
EP  - 362
IS  - 4
SP  - 357
VL  - 13
DO  - 10.3109/17482968.2012.665929
ER  - 

@article{
author = "Ignjatović, Aleksandar and Stević, Zorica D and Lavrnic, Dragana S and Nikolić-Kokić, Aleksandra and Blagojević, Duško P and Spasić, Mihajlo and Spasojević, Ivan",
year = "2012",
abstract = "ALS is characterized by oxidative damage in the brain and cerebrospinal fluid, which is exerted by pro-oxidative activity of iron. Such activity of iron can be drastically increased in the presence of inappropriate iron ligands that catalyze redox cycling of iron, thereby promoting hydroxyl radical generation. The aim of our study was to determine the relative level of inappropriate iron ligands in the cerebrospinal fluid of ALS patients. To determine the levels of inappropriate iron ligands and redox activity of iron in cerebrospinal fluid (10 samples from ALS patients and 10 controls), we applied electron paramagnetic resonance spectroscopy. We have shown that cerebrospinal fluid of ALS patients comprises twofold increased level of inappropriate iron ligands, proportionally increasing iron redox activity and hydroxyl radical production compared to controls. In conclusion, our results strongly support the pro-oxidative/detrimental role of inappropriately chelated iron in ALS pathophysiology. The identification of biomolecules that form such iron complexes and their therapeutic targeting may represent the future of ALS treatment.",
publisher = "Informa Healthcare, London",
journal = "Amyotrophic Lateral Sclerosis",
title = "Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
pages = "362-357",
number = "4",
volume = "13",
doi = "10.3109/17482968.2012.665929"
}

Ignjatović, A., Stević, Z. D., Lavrnic, D. S., Nikolić-Kokić, A., Blagojević, D. P., Spasić, M.,& Spasojević, I.. (2012). Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Amyotrophic Lateral Sclerosis
Informa Healthcare, London., 13(4), 357-362.
https://doi.org/10.3109/17482968.2012.665929

Ignjatović A, Stević ZD, Lavrnic DS, Nikolić-Kokić A, Blagojević DP, Spasić M, Spasojević I. Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Amyotrophic Lateral Sclerosis. 2012;13(4):357-362.
doi:10.3109/17482968.2012.665929 .

Ignjatović, Aleksandar, Stević, Zorica D, Lavrnic, Dragana S, Nikolić-Kokić, Aleksandra, Blagojević, Duško P, Spasić, Mihajlo, Spasojević, Ivan, "Inappropriately chelated iron in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Amyotrophic Lateral Sclerosis, 13, no. 4 (2012):357-362,
https://doi.org/10.3109/17482968.2012.665929 . .

TY  - JOUR
AU  - Bajić, Aleksandar
AU  - Zakrzewska, Joanna
AU  - Godjevac, Dejan M
AU  - Andjus, Pavle R.
AU  - Jones, David R
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/484
AB  - The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Research
T1  - Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions
EP  - 420
IS  - 3
SP  - 416
VL  - 346
DO  - 10.1016/j.carres.2010.12.008
ER  - 

@article{
author = "Bajić, Aleksandar and Zakrzewska, Joanna and Godjevac, Dejan M and Andjus, Pavle R. and Jones, David R and Spasić, Mihajlo and Spasojević, Ivan",
year = "2011",
abstract = "The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Research",
title = "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions",
pages = "420-416",
number = "3",
volume = "346",
doi = "10.1016/j.carres.2010.12.008"
}

Bajić, A., Zakrzewska, J., Godjevac, D. M., Andjus, P. R., Jones, D. R., Spasić, M.,& Spasojević, I.. (2011). Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research
Elsevier Sci Ltd, Oxford., 346(3), 416-420.
https://doi.org/10.1016/j.carres.2010.12.008

Bajić A, Zakrzewska J, Godjevac DM, Andjus PR, Jones DR, Spasić M, Spasojević I. Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research. 2011;346(3):416-420.
doi:10.1016/j.carres.2010.12.008 .

Bajić, Aleksandar, Zakrzewska, Joanna, Godjevac, Dejan M, Andjus, Pavle R., Jones, David R, Spasić, Mihajlo, Spasojević, Ivan, "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions" in Carbohydrate Research, 346, no. 3 (2011):416-420,
https://doi.org/10.1016/j.carres.2010.12.008 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Stević, Zorica D
AU  - Spasic, Snežana D
AU  - Jones, David R
AU  - Morina, Arian
AU  - Spasić, Mihajlo
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/417
AB  - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
PB  - Maney Publishing, Leeds
T2  - Redox Report
T1  - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
EP  - 35
IS  - 1
SP  - 29
VL  - 15
DO  - 10.1179/174329210X12650506623087
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Stević, Zorica D and Spasic, Snežana D and Jones, David R and Morina, Arian and Spasić, Mihajlo",
year = "2010",
abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.",
publisher = "Maney Publishing, Leeds",
journal = "Redox Report",
title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid",
pages = "35-29",
number = "1",
volume = "15",
doi = "10.1179/174329210X12650506623087"
}

Spasojević, I., Mojović, M., Stević, Z. D., Spasic, S. D., Jones, D. R., Morina, A.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report
Maney Publishing, Leeds., 15(1), 29-35.
https://doi.org/10.1179/174329210X12650506623087

Spasojević I, Mojović M, Stević ZD, Spasic SD, Jones DR, Morina A, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):29-35.
doi:10.1179/174329210X12650506623087 .

Spasojević, Ivan, Mojović, Miloš, Stević, Zorica D, Spasic, Snežana D, Jones, David R, Morina, Arian, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010):29-35,
https://doi.org/10.1179/174329210X12650506623087 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Stević, Zorica D
AU  - Nikolić-Kokić, Aleksandra
AU  - Jones, David R
AU  - Blagojević, Duško P
AU  - Spasić, Mihajlo
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/410
AB  - Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H2O2, which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H2O2 to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Redox Report
T1  - Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
EP  - 86
IS  - 2
SP  - 81
VL  - 15
DO  - 10.1179/174329210X12650506623320
ER  - 

@article{
author = "Spasojević, Ivan and Stević, Zorica D and Nikolić-Kokić, Aleksandra and Jones, David R and Blagojević, Duško P and Spasić, Mihajlo",
year = "2010",
abstract = "Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H2O2, which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H2O2 to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Redox Report",
title = "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
pages = "86-81",
number = "2",
volume = "15",
doi = "10.1179/174329210X12650506623320"
}

Spasojević, I., Stević, Z. D., Nikolić-Kokić, A., Jones, D. R., Blagojević, D. P.,& Spasić, M.. (2010). Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report
Taylor & Francis Ltd, Abingdon., 15(2), 81-86.
https://doi.org/10.1179/174329210X12650506623320

Spasojević I, Stević ZD, Nikolić-Kokić A, Jones DR, Blagojević DP, Spasić M. Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report. 2010;15(2):81-86.
doi:10.1179/174329210X12650506623320 .

Spasojević, Ivan, Stević, Zorica D, Nikolić-Kokić, Aleksandra, Jones, David R, Blagojević, Duško P, Spasić, Mihajlo, "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Redox Report, 15, no. 2 (2010):81-86,
https://doi.org/10.1179/174329210X12650506623320 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Blagojević, Duško
AU  - Spasic, Snežana D.
AU  - Jones, David R
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/321
AB  - The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Research
T1  - Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical
EP  - 84
IS  - 1
SP  - 80
VL  - 344
DO  - 10.1016/j.carres.2008.09.025
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Spasic, Snežana D. and Jones, David R and Nikolić-Kokić, Aleksandra and Spasić, Mihajlo",
year = "2009",
abstract = "The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Research",
title = "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical",
pages = "84-80",
number = "1",
volume = "344",
doi = "10.1016/j.carres.2008.09.025"
}

Spasojević, I., Mojović, M., Blagojević, D., Spasic, S. D., Jones, D. R., Nikolić-Kokić, A.,& Spasić, M.. (2009). Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research
Elsevier Sci Ltd, Oxford., 344(1), 80-84.
https://doi.org/10.1016/j.carres.2008.09.025

Spasojević I, Mojović M, Blagojević D, Spasic SD, Jones DR, Nikolić-Kokić A, Spasić M. Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research. 2009;344(1):80-84.
doi:10.1016/j.carres.2008.09.025 .

Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Spasic, Snežana D., Jones, David R, Nikolić-Kokić, Aleksandra, Spasić, Mihajlo, "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical" in Carbohydrate Research, 344, no. 1 (2009):80-84,
https://doi.org/10.1016/j.carres.2008.09.025 . .

TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Spasojević, Ivan
AU  - Miković, Zeljko
AU  - Mandic, Vesna
AU  - Cerović, Nikola
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/330
AB  - Our aim was to investigate the activities of antioxidative defense enzymes in the placenta, fetal blood and amnion fluid in inherited thrombophilia. Thrombophilia was associated with nearly threefold increase of activity (p  lt  0.001) of the placental catalase (81.1 +/- 20.6 U/mg of proteins in controls and 270.0 +/- 69.9 U/mg in thrombophilic subjects), glutathione (GSH) peroxidase (C: 20.2 +/- 10.1 U/mg; T. 60.0 +/- 15.5 U/mg), and GSH reductase (C: 28.9 +/- 5.6 U/mg; T. 72.7 +/- 23.0 U/mg). The placental activities of superoxide dismutating enzymes-MnSOD and CuZnSOD, did not differ in controls and thrombophilia. Likewise, the activities of catalase and SOD in the fetal blood, and the level of ascorbyl radical which represents a marker of oxidative status of amniotic fluid, were similar in controls and thrombophilic subjects. From this we concluded that in thrombophilia, placental tissue is exposed to H2O2-mediated oxidative stress, which could be initiated by pro-thrombic conditions in maternal blood. Increased activity of placental H2O2-removing enzymes protects fetus and mother during pregnancy, but may increase the risk of postpartum thrombosis.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide
EP  - 18
IS  - 1
SP  - 14
VL  - 2
DO  - 10.4161/oxim.2.1.7705
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Spasojević, Ivan and Miković, Zeljko and Mandic, Vesna and Cerović, Nikola and Spasić, Mihajlo",
year = "2009",
abstract = "Our aim was to investigate the activities of antioxidative defense enzymes in the placenta, fetal blood and amnion fluid in inherited thrombophilia. Thrombophilia was associated with nearly threefold increase of activity (p  lt  0.001) of the placental catalase (81.1 +/- 20.6 U/mg of proteins in controls and 270.0 +/- 69.9 U/mg in thrombophilic subjects), glutathione (GSH) peroxidase (C: 20.2 +/- 10.1 U/mg; T. 60.0 +/- 15.5 U/mg), and GSH reductase (C: 28.9 +/- 5.6 U/mg; T. 72.7 +/- 23.0 U/mg). The placental activities of superoxide dismutating enzymes-MnSOD and CuZnSOD, did not differ in controls and thrombophilia. Likewise, the activities of catalase and SOD in the fetal blood, and the level of ascorbyl radical which represents a marker of oxidative status of amniotic fluid, were similar in controls and thrombophilic subjects. From this we concluded that in thrombophilia, placental tissue is exposed to H2O2-mediated oxidative stress, which could be initiated by pro-thrombic conditions in maternal blood. Increased activity of placental H2O2-removing enzymes protects fetus and mother during pregnancy, but may increase the risk of postpartum thrombosis.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide",
pages = "18-14",
number = "1",
volume = "2",
doi = "10.4161/oxim.2.1.7705"
}

Bogdanović Pristov, J., Spasojević, I., Miković, Z., Mandic, V., Cerović, N.,& Spasić, M.. (2009). Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2(1), 14-18.
https://doi.org/10.4161/oxim.2.1.7705

Bogdanović Pristov J, Spasojević I, Miković Z, Mandic V, Cerović N, Spasić M. Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide. in Oxidative Medicine and Cellular Longevity. 2009;2(1):14-18.
doi:10.4161/oxim.2.1.7705 .

Bogdanović Pristov, Jelena, Spasojević, Ivan, Miković, Zeljko, Mandic, Vesna, Cerović, Nikola, Spasić, Mihajlo, "Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide" in Oxidative Medicine and Cellular Longevity, 2, no. 1 (2009):14-18,
https://doi.org/10.4161/oxim.2.1.7705 . .

TY  - JOUR
AU  - Kojic, Danijela
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Blagojević, Duško
AU  - Worland, M. Roger
AU  - Grubor-Lajsic, Gordana
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/328
AB  - The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.
PB  - Czech Acad Sci, Inst Entomology, Ceske Budejovice
T2  - European Journal of Entomology
T1  - Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)
EP  - 454
IS  - 3
SP  - 451
VL  - 106
DO  - 10.14411/eje.2009.056
ER  - 

@article{
author = "Kojic, Danijela and Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Worland, M. Roger and Grubor-Lajsic, Gordana and Spasić, Mihajlo",
year = "2009",
abstract = "The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.",
publisher = "Czech Acad Sci, Inst Entomology, Ceske Budejovice",
journal = "European Journal of Entomology",
title = "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)",
pages = "454-451",
number = "3",
volume = "106",
doi = "10.14411/eje.2009.056"
}

Kojic, D., Spasojević, I., Mojović, M., Blagojević, D., Worland, M. R., Grubor-Lajsic, G.,& Spasić, M.. (2009). Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology
Czech Acad Sci, Inst Entomology, Ceske Budejovice., 106(3), 451-454.
https://doi.org/10.14411/eje.2009.056

Kojic D, Spasojević I, Mojović M, Blagojević D, Worland MR, Grubor-Lajsic G, Spasić M. Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology. 2009;106(3):451-454.
doi:10.14411/eje.2009.056 .

Kojic, Danijela, Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Worland, M. Roger, Grubor-Lajsic, Gordana, Spasić, Mihajlo, "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)" in European Journal of Entomology, 106, no. 3 (2009):451-454,
https://doi.org/10.14411/eje.2009.056 . .

TY  - JOUR
AU  - Oreščanin-Dušić, Zorana
AU  - Milovanović, Slobodan
AU  - Blagojević, Duško
AU  - Nikolić-Kokić, Aleksandra
AU  - Radojicic, Ratko
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/335
AB  - Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC2 complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC2 complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Redox Report
T1  - Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus
EP  - 54
IS  - 2
SP  - 48
VL  - 14
DO  - 10.1179/135100009X392476
ER  - 

@article{
author = "Oreščanin-Dušić, Zorana and Milovanović, Slobodan and Blagojević, Duško and Nikolić-Kokić, Aleksandra and Radojicic, Ratko and Spasojević, Ivan and Spasić, Mihajlo",
year = "2009",
abstract = "Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC2 complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC2 complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Redox Report",
title = "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus",
pages = "54-48",
number = "2",
volume = "14",
doi = "10.1179/135100009X392476"
}

Oreščanin-Dušić, Z., Milovanović, S., Blagojević, D., Nikolić-Kokić, A., Radojicic, R., Spasojević, I.,& Spasić, M.. (2009). Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report
Taylor & Francis Ltd, Abingdon., 14(2), 48-54.
https://doi.org/10.1179/135100009X392476

Oreščanin-Dušić Z, Milovanović S, Blagojević D, Nikolić-Kokić A, Radojicic R, Spasojević I, Spasić M. Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report. 2009;14(2):48-54.
doi:10.1179/135100009X392476 .

Oreščanin-Dušić, Zorana, Milovanović, Slobodan, Blagojević, Duško, Nikolić-Kokić, Aleksandra, Radojicic, Ratko, Spasojević, Ivan, Spasić, Mihajlo, "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus" in Redox Report, 14, no. 2 (2009):48-54,
https://doi.org/10.1179/135100009X392476 . .

TY  - JOUR
AU  - Mojović, Miloš
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
AU  - Bačić, Goran
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/167
PB  - Taylor & Francis Ltd, Abingdon
T2  - Free Radical Research
T1  - Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems
EP  - S72
SP  - S72
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_167
ER  - 

@article{
author = "Mojović, Miloš and Spasojević, Ivan and Spasić, Mihajlo and Bačić, Goran",
year = "2006",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Free Radical Research",
title = "Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems",
pages = "S72-S72",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_167"
}

Mojović, M., Spasojević, I., Spasić, M.,& Bačić, G.. (2006). Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems. in Free Radical Research
Taylor & Francis Ltd, Abingdon., 40, S72-S72.
https://hdl.handle.net/21.15107/rcub_rimsi_167

Mojović M, Spasojević I, Spasić M, Bačić G. Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems. in Free Radical Research. 2006;40:S72-S72.
https://hdl.handle.net/21.15107/rcub_rimsi_167 .

Mojović, Miloš, Spasojević, Ivan, Spasić, Mihajlo, Bačić, Goran, "Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems" in Free Radical Research, 40 (2006):S72-S72,
https://hdl.handle.net/21.15107/rcub_rimsi_167 .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Stević, Z.
AU  - Batas, V.
AU  - Bačić, Goran
AU  - Spasić, Mihajlo
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/164
PB  - Taylor & Francis Ltd, Abingdon
T2  - Free Radical Research
T1  - Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS
EP  - S90
SP  - S90
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_164
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Stević, Z. and Batas, V. and Bačić, Goran and Spasić, Mihajlo",
year = "2006",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Free Radical Research",
title = "Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS",
pages = "S90-S90",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_164"
}

Spasojević, I., Mojović, M., Stević, Z., Batas, V., Bačić, G.,& Spasić, M.. (2006). Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS. in Free Radical Research
Taylor & Francis Ltd, Abingdon., 40, S90-S90.
https://hdl.handle.net/21.15107/rcub_rimsi_164

Spasojević I, Mojović M, Stević Z, Batas V, Bačić G, Spasić M. Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS. in Free Radical Research. 2006;40:S90-S90.
https://hdl.handle.net/21.15107/rcub_rimsi_164 .

Spasojević, Ivan, Mojović, Miloš, Stević, Z., Batas, V., Bačić, Goran, Spasić, Mihajlo, "Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS" in Free Radical Research, 40 (2006):S90-S90,
https://hdl.handle.net/21.15107/rcub_rimsi_164 .