TY  - JOUR
AU  - Parmar, Priyanka
AU  - Villalba, Maria Ines
AU  - Seiji, Alexandre
AU  - Huber, Horii
AU  - Kalauzi, Aleksandar
AU  - Bartolić, Dragana
AU  - Radotić, Ksenija
AU  - Willaert, Ronnie
AU  - MacFabe, Derrick
AU  - Kasas, Sandor
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2183
AB  - Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sensitivity, and is label- and attachment-free.
T2  - Frontiers in Microbiology
T1  - Mitochondrial nanomotion measured by optical microscopy
IS  - 1133773
VL  - 14
DO  - 10.3389/fmicb.2023.1133773
ER  - 

@article{
author = "Parmar, Priyanka and Villalba, Maria Ines and Seiji, Alexandre and Huber, Horii and Kalauzi, Aleksandar and Bartolić, Dragana and Radotić, Ksenija and Willaert, Ronnie and MacFabe, Derrick and Kasas, Sandor",
year = "2023",
abstract = "Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sensitivity, and is label- and attachment-free.",
journal = "Frontiers in Microbiology",
title = "Mitochondrial nanomotion measured by optical microscopy",
number = "1133773",
volume = "14",
doi = "10.3389/fmicb.2023.1133773"
}

Parmar, P., Villalba, M. I., Seiji, A., Huber, H., Kalauzi, A., Bartolić, D., Radotić, K., Willaert, R., MacFabe, D.,& Kasas, S.. (2023). Mitochondrial nanomotion measured by optical microscopy. in Frontiers in Microbiology, 14(1133773).
https://doi.org/10.3389/fmicb.2023.1133773

Parmar P, Villalba MI, Seiji A, Huber H, Kalauzi A, Bartolić D, Radotić K, Willaert R, MacFabe D, Kasas S. Mitochondrial nanomotion measured by optical microscopy. in Frontiers in Microbiology. 2023;14(1133773).
doi:10.3389/fmicb.2023.1133773 .

Parmar, Priyanka, Villalba, Maria Ines, Seiji, Alexandre, Huber, Horii, Kalauzi, Aleksandar, Bartolić, Dragana, Radotić, Ksenija, Willaert, Ronnie, MacFabe, Derrick, Kasas, Sandor, "Mitochondrial nanomotion measured by optical microscopy" in Frontiers in Microbiology, 14, no. 1133773 (2023),
https://doi.org/10.3389/fmicb.2023.1133773 . .

TY  - JOUR
AU  - Willaert, Ronnie
AU  - Vanden, Boer, Pieterjan
AU  - Malovichko, Anton
AU  - Alioscha-Perez, Mitchel
AU  - Radotić, Ksenija
AU  - Bartolić, Dragana
AU  - Kalauzi, Aleksandar
AU  - Villalba, Maria Ines
AU  - Sanglard, Dominique
AU  - Dietler, Giovanni
AU  - Sahli, Hichem
AU  - Kasas, Sandor
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1335
AB  - Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.
PB  - Amer Assoc Advancement Science, Washington
T2  - Science Advances
T1  - Single yeast cell nanomotions correlate with cellular activity
IS  - 26
VL  - 6
DO  - 10.1126/sciadv.aba3139
ER  - 

@article{
author = "Willaert, Ronnie and Vanden, Boer, Pieterjan and Malovichko, Anton and Alioscha-Perez, Mitchel and Radotić, Ksenija and Bartolić, Dragana and Kalauzi, Aleksandar and Villalba, Maria Ines and Sanglard, Dominique and Dietler, Giovanni and Sahli, Hichem and Kasas, Sandor",
year = "2020",
abstract = "Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.",
publisher = "Amer Assoc Advancement Science, Washington",
journal = "Science Advances",
title = "Single yeast cell nanomotions correlate with cellular activity",
number = "26",
volume = "6",
doi = "10.1126/sciadv.aba3139"
}

Willaert, R., Vanden, B. P., Malovichko, A., Alioscha-Perez, M., Radotić, K., Bartolić, D., Kalauzi, A., Villalba, M. I., Sanglard, D., Dietler, G., Sahli, H.,& Kasas, S.. (2020). Single yeast cell nanomotions correlate with cellular activity. in Science Advances
Amer Assoc Advancement Science, Washington., 6(26).
https://doi.org/10.1126/sciadv.aba3139

Willaert R, Vanden BP, Malovichko A, Alioscha-Perez M, Radotić K, Bartolić D, Kalauzi A, Villalba MI, Sanglard D, Dietler G, Sahli H, Kasas S. Single yeast cell nanomotions correlate with cellular activity. in Science Advances. 2020;6(26).
doi:10.1126/sciadv.aba3139 .

Willaert, Ronnie, Vanden, Boer, Pieterjan, Malovichko, Anton, Alioscha-Perez, Mitchel, Radotić, Ksenija, Bartolić, Dragana, Kalauzi, Aleksandar, Villalba, Maria Ines, Sanglard, Dominique, Dietler, Giovanni, Sahli, Hichem, Kasas, Sandor, "Single yeast cell nanomotions correlate with cellular activity" in Science Advances, 6, no. 26 (2020),
https://doi.org/10.1126/sciadv.aba3139 . .

TY  - JOUR
AU  - Venturelli, Leonardo
AU  - Kohler, Anne-Celine
AU  - Stupar, Petar
AU  - Villalba, Maria, I
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
AU  - Bertacchi, Massimiliano
AU  - Dinarelli, Simone
AU  - Girasole, Marco
AU  - Pesic, Milica
AU  - Banković, Jasna
AU  - Vela, Maria E.
AU  - Yantorno, Osvaldo
AU  - Willaert, Ronnie
AU  - Dietler, Giovanni
AU  - Longo, Giovanni
AU  - Kasas, Sandor
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1338
AB  - The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.
PB  - Wiley, Hoboken
T2  - Journal of Molecular Recognition
T1  - A perspective view on the nanomotion detection of living organisms and its features
IS  - 12
VL  - 33
DO  - 10.1002/jmr.2849
ER  - 

@article{
author = "Venturelli, Leonardo and Kohler, Anne-Celine and Stupar, Petar and Villalba, Maria, I and Kalauzi, Aleksandar and Radotić, Ksenija and Bertacchi, Massimiliano and Dinarelli, Simone and Girasole, Marco and Pesic, Milica and Banković, Jasna and Vela, Maria E. and Yantorno, Osvaldo and Willaert, Ronnie and Dietler, Giovanni and Longo, Giovanni and Kasas, Sandor",
year = "2020",
abstract = "The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.",
publisher = "Wiley, Hoboken",
journal = "Journal of Molecular Recognition",
title = "A perspective view on the nanomotion detection of living organisms and its features",
number = "12",
volume = "33",
doi = "10.1002/jmr.2849"
}

Venturelli, L., Kohler, A., Stupar, P., Villalba, M. I., Kalauzi, A., Radotić, K., Bertacchi, M., Dinarelli, S., Girasole, M., Pesic, M., Banković, J., Vela, M. E., Yantorno, O., Willaert, R., Dietler, G., Longo, G.,& Kasas, S.. (2020). A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition
Wiley, Hoboken., 33(12).
https://doi.org/10.1002/jmr.2849

Venturelli L, Kohler A, Stupar P, Villalba MI, Kalauzi A, Radotić K, Bertacchi M, Dinarelli S, Girasole M, Pesic M, Banković J, Vela ME, Yantorno O, Willaert R, Dietler G, Longo G, Kasas S. A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition. 2020;33(12).
doi:10.1002/jmr.2849 .

Venturelli, Leonardo, Kohler, Anne-Celine, Stupar, Petar, Villalba, Maria, I, Kalauzi, Aleksandar, Radotić, Ksenija, Bertacchi, Massimiliano, Dinarelli, Simone, Girasole, Marco, Pesic, Milica, Banković, Jasna, Vela, Maria E., Yantorno, Osvaldo, Willaert, Ronnie, Dietler, Giovanni, Longo, Giovanni, Kasas, Sandor, "A perspective view on the nanomotion detection of living organisms and its features" in Journal of Molecular Recognition, 33, no. 12 (2020),
https://doi.org/10.1002/jmr.2849 . .