TY  - JOUR
AU  - Milojkov, Dušan
AU  - Radosavljević-Mihajlović, Ana S
AU  - Stanić, Vojislav Dj
AU  - Nastasijević, Branislav
AU  - Radotić, Ksenija
AU  - Janković Častvan, Ivona
AU  - Živković-Radovanović, Vukosava
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2649
AB  - Nanomaterials based on metal–doped fluorapatite (FAP) have attracted considerable interest as potential
next–generation antimicrobial agents. In this study, Cu2+–doped FAP nanocrystals have been successfully synthesized
by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible
properties have been investigated. XRD, FTIR, FESEM, and N2 adsorption–desorption studies indicate
the formation of single–phase FAP mesoporous nanopowders, composed of rod–like particles. TEM images
confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis
shows that the Cu2+ ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure.
Fluorescence spectroscopy accompanied by MCR–ALS method confirms substitution of Cu2+ ions in FAP crystal
lattice with extracting additional d–d band transition at green color from FAP broadband self–activated luminescence
in violet–blue color. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and
Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu2+ content have strong bacteriostatic
action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample
in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three
species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium
growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of
Aspergillus niger species. Cu2+–doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu2+ and F�����
ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu2+–doped FAP samples
were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad–
spectrum antimicrobial agents for various biomedical and health care preparations.
PB  - Elsevier
T2  - Journal of Photochemistry & Photobiology, B: Biology
T1  - Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents
SP  - 112649
VL  - 239
DO  - 10.1016/j.jphotobiol.2023.112649
ER  - 

@article{
author = "Milojkov, Dušan and Radosavljević-Mihajlović, Ana S and Stanić, Vojislav Dj and Nastasijević, Branislav and Radotić, Ksenija and Janković Častvan, Ivona and Živković-Radovanović, Vukosava",
year = "2023",
abstract = "Nanomaterials based on metal–doped fluorapatite (FAP) have attracted considerable interest as potential
next–generation antimicrobial agents. In this study, Cu2+–doped FAP nanocrystals have been successfully synthesized
by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible
properties have been investigated. XRD, FTIR, FESEM, and N2 adsorption–desorption studies indicate
the formation of single–phase FAP mesoporous nanopowders, composed of rod–like particles. TEM images
confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis
shows that the Cu2+ ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure.
Fluorescence spectroscopy accompanied by MCR–ALS method confirms substitution of Cu2+ ions in FAP crystal
lattice with extracting additional d–d band transition at green color from FAP broadband self–activated luminescence
in violet–blue color. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and
Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu2+ content have strong bacteriostatic
action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample
in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three
species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium
growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of
Aspergillus niger species. Cu2+–doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu2+ and F�����
ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu2+–doped FAP samples
were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad–
spectrum antimicrobial agents for various biomedical and health care preparations.",
publisher = "Elsevier",
journal = "Journal of Photochemistry & Photobiology, B: Biology",
title = "Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents",
pages = "112649",
volume = "239",
doi = "10.1016/j.jphotobiol.2023.112649"
}

Milojkov, D., Radosavljević-Mihajlović, A. S., Stanić, V. D., Nastasijević, B., Radotić, K., Janković Častvan, I.,& Živković-Radovanović, V.. (2023). Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents. in Journal of Photochemistry & Photobiology, B: Biology
Elsevier., 239, 112649.
https://doi.org/10.1016/j.jphotobiol.2023.112649

Milojkov D, Radosavljević-Mihajlović AS, Stanić VD, Nastasijević B, Radotić K, Janković Častvan I, Živković-Radovanović V. Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents. in Journal of Photochemistry & Photobiology, B: Biology. 2023;239:112649.
doi:10.1016/j.jphotobiol.2023.112649 .

Milojkov, Dušan, Radosavljević-Mihajlović, Ana S, Stanić, Vojislav Dj, Nastasijević, Branislav, Radotić, Ksenija, Janković Častvan, Ivona, Živković-Radovanović, Vukosava, "Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents" in Journal of Photochemistry & Photobiology, B: Biology, 239 (2023):112649,
https://doi.org/10.1016/j.jphotobiol.2023.112649 . .