TY  - JOUR
AU  - Parmar, Priyanka
AU  - Villalba, Maria Ines
AU  - Seiji, Alexandre
AU  - Huber, Horii
AU  - Kalauzi, Aleksandar
AU  - Bartolić, Dragana
AU  - Radotić, Ksenija
AU  - Willaert, Ronnie
AU  - MacFabe, Derrick
AU  - Kasas, Sandor
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2183
AB  - Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sensitivity, and is label- and attachment-free.
T2  - Frontiers in Microbiology
T1  - Mitochondrial nanomotion measured by optical microscopy
IS  - 1133773
VL  - 14
DO  - 10.3389/fmicb.2023.1133773
ER  - 

@article{
author = "Parmar, Priyanka and Villalba, Maria Ines and Seiji, Alexandre and Huber, Horii and Kalauzi, Aleksandar and Bartolić, Dragana and Radotić, Ksenija and Willaert, Ronnie and MacFabe, Derrick and Kasas, Sandor",
year = "2023",
abstract = "Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sensitivity, and is label- and attachment-free.",
journal = "Frontiers in Microbiology",
title = "Mitochondrial nanomotion measured by optical microscopy",
number = "1133773",
volume = "14",
doi = "10.3389/fmicb.2023.1133773"
}

Parmar, P., Villalba, M. I., Seiji, A., Huber, H., Kalauzi, A., Bartolić, D., Radotić, K., Willaert, R., MacFabe, D.,& Kasas, S.. (2023). Mitochondrial nanomotion measured by optical microscopy. in Frontiers in Microbiology, 14(1133773).
https://doi.org/10.3389/fmicb.2023.1133773

Parmar P, Villalba MI, Seiji A, Huber H, Kalauzi A, Bartolić D, Radotić K, Willaert R, MacFabe D, Kasas S. Mitochondrial nanomotion measured by optical microscopy. in Frontiers in Microbiology. 2023;14(1133773).
doi:10.3389/fmicb.2023.1133773 .

Parmar, Priyanka, Villalba, Maria Ines, Seiji, Alexandre, Huber, Horii, Kalauzi, Aleksandar, Bartolić, Dragana, Radotić, Ksenija, Willaert, Ronnie, MacFabe, Derrick, Kasas, Sandor, "Mitochondrial nanomotion measured by optical microscopy" in Frontiers in Microbiology, 14, no. 1133773 (2023),
https://doi.org/10.3389/fmicb.2023.1133773 . .

TY  - CONF
AU  - Radotić, Ksenija
AU  - Roduit, Charles
AU  - Kasas, Sandor
AU  - Simonović, Jasna
AU  - Bogdanović Pristov, Jelena
AU  - Đikanović, Daniela
AU  - Dietler, Giovanni
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3027
C3  - Cost action FP 0802, Workshop „Wood structure/function-relationships“, Hamburg, Germany
T1  - Stiffness of the isolated arabidopsis cell wall during soaking
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3027
ER  - 

@conference{
author = "Radotić, Ksenija and Roduit, Charles and Kasas, Sandor and Simonović, Jasna and Bogdanović Pristov, Jelena and Đikanović, Daniela and Dietler, Giovanni",
year = "2010",
journal = "Cost action FP 0802, Workshop „Wood structure/function-relationships“, Hamburg, Germany",
title = "Stiffness of the isolated arabidopsis cell wall during soaking",
pages = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3027"
}

Radotić, K., Roduit, C., Kasas, S., Simonović, J., Bogdanović Pristov, J., Đikanović, D.,& Dietler, G.. (2010). Stiffness of the isolated arabidopsis cell wall during soaking. in Cost action FP 0802, Workshop „Wood structure/function-relationships“, Hamburg, Germany, 45.
https://hdl.handle.net/21.15107/rcub_rimsi_3027

Radotić K, Roduit C, Kasas S, Simonović J, Bogdanović Pristov J, Đikanović D, Dietler G. Stiffness of the isolated arabidopsis cell wall during soaking. in Cost action FP 0802, Workshop „Wood structure/function-relationships“, Hamburg, Germany. 2010;:45.
https://hdl.handle.net/21.15107/rcub_rimsi_3027 .

Radotić, Ksenija, Roduit, Charles, Kasas, Sandor, Simonović, Jasna, Bogdanović Pristov, Jelena, Đikanović, Daniela, Dietler, Giovanni, "Stiffness of the isolated arabidopsis cell wall during soaking" in Cost action FP 0802, Workshop „Wood structure/function-relationships“, Hamburg, Germany (2010):45,
https://hdl.handle.net/21.15107/rcub_rimsi_3027 .