TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kilsey
AU  - Oswald, Petar
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2044
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
EP  - 32
IS  - 1
SP  - 21
VL  - 38
DO  - 10.1093/mutage/geac024
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kilsey and Oswald, Petar and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
pages = "32-21",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024"
}

Jovanović Marić, J., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević Aleksić J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kilsey, Oswald, Petar, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - JOUR
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Oalđe Pavlović, Mariana
AU  - Sladić, Dušan
AU  - Novaković, Irena
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2018
AB  - Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.
PB  - National Library of Serbia
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone
EP  - 1258
IS  - 11
SP  - 1245
VL  - 87
DO  - 10.2298/JSC220304044D
ER  - 

@article{
author = "Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović Marić, Jovana and Oalđe Pavlović, Mariana and Sladić, Dušan and Novaković, Irena and Vuković-Gačić, Branka",
year = "2022",
abstract = "Biological activity of 2-tert-butyl-1,4-benzoquinone (TBQ) and its derivatives, 2-tert-butyl-5-(2-propylthio)-1,4-benzoquinone, 2-tert-butyl-5- -(propylthio)-1,4-benzoquinone, 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone, 2-tert-butyl-5-(phenylthio)-1,4-benzoquinone and 2-tert-butyl-6-(phenylthio)- 1,4-benzoquinone, were tested for their antioxidant, antibacterial, toxic, cytotoxic and genotoxic potential. Using the DPPH test, all derivatives showed good antioxidant activity, better than ascorbic acid, and the 2-tert- -butyl-5-(propylthio)-1,4-benzoquinone derivative showed the strongest effect. Better antibacterial potential was observed against Gram-positive bacteria in the broth microdilution method in which the 2-tert-butyl-5-(phenylthio)-1,4- -benzoquinone derivative showed the strongest activity (MIC = 15.6 μM). The results of toxicity tests, using the Brine shrimp test, indicated that the derivatives lose their toxic potential compared to TBQ, except for 2-tert-butyl-6- -(phenylthio)-1,4-benzoquinone, which showed a 3 times stronger effect. Cytotoxicity was assessed by the MTT assay in 24 and 72 h treatments in MRC-5, HS 294T and A549 cell lines in threefold decreasing gradient (11, 33 and 100 μM). Modifications potentiate the cytotoxic effect, and the strongest effect was observed with the 2-tert-butyl-5,6-(ethylendithio)-1,4-benzoquinone derivative. In addition, the genotoxic potential was examined in the MRC-5 cell line using the comet assay. All tested derivatives of TBQ showed a genotoxic effect at all applied subtoxic concentrations. In general, the chemical modifications of TBQ enhanced its biological activity.",
publisher = "National Library of Serbia",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone",
pages = "1258-1245",
number = "11",
volume = "87",
doi = "10.2298/JSC220304044D"
}

Đorđević Aleksić, J., Kolarević, S., Jovanović Marić, J., Oalđe Pavlović, M., Sladić, D., Novaković, I.,& Vuković-Gačić, B.. (2022). Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society
National Library of Serbia., 87(11), 1245-1258.
https://doi.org/10.2298/JSC220304044D

Đorđević Aleksić J, Kolarević S, Jovanović Marić J, Oalđe Pavlović M, Sladić D, Novaković I, Vuković-Gačić B. Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone. in Journal of the Serbian Chemical Society. 2022;87(11):1245-1258.
doi:10.2298/JSC220304044D .

Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović Marić, Jovana, Oalđe Pavlović, Mariana, Sladić, Dušan, Novaković, Irena, Vuković-Gačić, Branka, "Synthesis and biological activity of alkylthio and arylthio derivatives of tert-butylquinone" in Journal of the Serbian Chemical Society, 87, no. 11 (2022):1245-1258,
https://doi.org/10.2298/JSC220304044D . .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kostić-Vuković, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Gacic, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1922
AB  - Narušavanje kvaliteta površinskih vodotokova otpadnim vodama se odražava na kvalitet akvatičnih ekosistema ali i na kvalitet života i zdravlja ljudi. Variranje u stepenu prerade otpadnih voda najčešće je posledica razlika u nacionalnim zakonskim regulativama što je veoma evidentno u slučaju reke Dunav, u nekim od podunavskih zemalja voda se prerađuje dok se u drugim nikakav tretman ne primenjuje pre ispuštanja. JDS3 (The Joint Danube Survey 3) je bila najveća rečna ekspedicija u 2013. godini koja je obuhvatila svih X sektora reke. Zbog obima uzoraka i različitih analiza, JDS je predstavljao idealnu priliku da se ispita prisustvo genotoksičnog zagađenja u ovoj reci. Komet test je rađen na hemocitama školjki Unio sp. i Sinanodanta woodiana. Najviši nivo genotoksičnog potencijala detektovan je u sektoru VI (Panonska ravan) gde veliki problem predstavljaju otpadne vode koje se bez prerade ispuštaju u Dunav. Ova studija je dala mapu zagađenja i kritičnih tačaka zagađenja duž reke Dunav i ukazala na bitnost prerade otpadnih voda za očuvanje i poboljšanje kvaliteta reke Dunav. Uvođenje molekularnih markera u monitoring kvaliteta voda znatno doprinosi razumevanju prirode i porekla zagađenja.
PB  - Srpsko biološko društvo
C3  - Drugi kongres biologa Srbije, Kladovo, Republika Srbija
T1  - Uticaj otpadnih voda na reku Dunav – ekogenotoksikološki aspekt ekspedicije JDS3
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1922
ER  - 

@conference{
author = "Kolarević, Stoimir and Kostić-Vuković, Jovana and Kračun-Kolarević, Margareta and Paunović, Momir and Gacic, Zoran and Vuković-Gačić, Branka",
year = "2018",
abstract = "Narušavanje kvaliteta površinskih vodotokova otpadnim vodama se odražava na kvalitet akvatičnih ekosistema ali i na kvalitet života i zdravlja ljudi. Variranje u stepenu prerade otpadnih voda najčešće je posledica razlika u nacionalnim zakonskim regulativama što je veoma evidentno u slučaju reke Dunav, u nekim od podunavskih zemalja voda se prerađuje dok se u drugim nikakav tretman ne primenjuje pre ispuštanja. JDS3 (The Joint Danube Survey 3) je bila najveća rečna ekspedicija u 2013. godini koja je obuhvatila svih X sektora reke. Zbog obima uzoraka i različitih analiza, JDS je predstavljao idealnu priliku da se ispita prisustvo genotoksičnog zagađenja u ovoj reci. Komet test je rađen na hemocitama školjki Unio sp. i Sinanodanta woodiana. Najviši nivo genotoksičnog potencijala detektovan je u sektoru VI (Panonska ravan) gde veliki problem predstavljaju otpadne vode koje se bez prerade ispuštaju u Dunav. Ova studija je dala mapu zagađenja i kritičnih tačaka zagađenja duž reke Dunav i ukazala na bitnost prerade otpadnih voda za očuvanje i poboljšanje kvaliteta reke Dunav. Uvođenje molekularnih markera u monitoring kvaliteta voda znatno doprinosi razumevanju prirode i porekla zagađenja.",
publisher = "Srpsko biološko društvo",
journal = "Drugi kongres biologa Srbije, Kladovo, Republika Srbija",
title = "Uticaj otpadnih voda na reku Dunav – ekogenotoksikološki aspekt ekspedicije JDS3",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1922"
}

Kolarević, S., Kostić-Vuković, J., Kračun-Kolarević, M., Paunović, M., Gacic, Z.,& Vuković-Gačić, B.. (2018). Uticaj otpadnih voda na reku Dunav – ekogenotoksikološki aspekt ekspedicije JDS3. in Drugi kongres biologa Srbije, Kladovo, Republika Srbija
Srpsko biološko društvo., 85.
https://hdl.handle.net/21.15107/rcub_rimsi_1922

Kolarević S, Kostić-Vuković J, Kračun-Kolarević M, Paunović M, Gacic Z, Vuković-Gačić B. Uticaj otpadnih voda na reku Dunav – ekogenotoksikološki aspekt ekspedicije JDS3. in Drugi kongres biologa Srbije, Kladovo, Republika Srbija. 2018;:85.
https://hdl.handle.net/21.15107/rcub_rimsi_1922 .

Kolarević, Stoimir, Kostić-Vuković, Jovana, Kračun-Kolarević, Margareta, Paunović, Momir, Gacic, Zoran, Vuković-Gačić, Branka, "Uticaj otpadnih voda na reku Dunav – ekogenotoksikološki aspekt ekspedicije JDS3" in Drugi kongres biologa Srbije, Kladovo, Republika Srbija (2018):85,
https://hdl.handle.net/21.15107/rcub_rimsi_1922 .

TY  - CONF
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Gačić, Zoran
AU  - Mićković, Branislav
AU  - Nikčević, Miroslav
AU  - Knežević-Vukčević, Jelena
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2574
PB  - Balkan Institute for Water and Environment - IB2E www.ib2e.org  Faculty of Civil Engineering (Sts. Cyril and Methodius University - Skopje) Hydrometeorological Service of Macedonia Macedonian Association of Meteorology METEO MAK, Makedonija
C3  - Balwois 2012
T1  - Ecogenotoxicity Analysis with Comet Assay in Different Tissues of Chub (Squalius cephalus L. 1758)
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2574
ER  - 

@conference{
author = "Sunjog, Karolina and Kolarević, Stoimir and Gačić, Zoran and Mićković, Branislav and Nikčević, Miroslav and Knežević-Vukčević, Jelena and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2012",
publisher = "Balkan Institute for Water and Environment - IB2E www.ib2e.org  Faculty of Civil Engineering (Sts. Cyril and Methodius University - Skopje) Hydrometeorological Service of Macedonia Macedonian Association of Meteorology METEO MAK, Makedonija",
journal = "Balwois 2012",
title = "Ecogenotoxicity Analysis with Comet Assay in Different Tissues of Chub (Squalius cephalus L. 1758)",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2574"
}

Sunjog, K., Kolarević, S., Gačić, Z., Mićković, B., Nikčević, M., Knežević-Vukčević, J., Lenhardt, M.,& Vuković-Gačić, B.. (2012). Ecogenotoxicity Analysis with Comet Assay in Different Tissues of Chub (Squalius cephalus L. 1758). in Balwois 2012
Balkan Institute for Water and Environment - IB2E www.ib2e.org  Faculty of Civil Engineering (Sts. Cyril and Methodius University - Skopje) Hydrometeorological Service of Macedonia Macedonian Association of Meteorology METEO MAK, Makedonija..
https://hdl.handle.net/21.15107/rcub_rimsi_2574

Sunjog K, Kolarević S, Gačić Z, Mićković B, Nikčević M, Knežević-Vukčević J, Lenhardt M, Vuković-Gačić B. Ecogenotoxicity Analysis with Comet Assay in Different Tissues of Chub (Squalius cephalus L. 1758). in Balwois 2012. 2012;.
https://hdl.handle.net/21.15107/rcub_rimsi_2574 .

Sunjog, Karolina, Kolarević, Stoimir, Gačić, Zoran, Mićković, Branislav, Nikčević, Miroslav, Knežević-Vukčević, Jelena, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Ecogenotoxicity Analysis with Comet Assay in Different Tissues of Chub (Squalius cephalus L. 1758)" in Balwois 2012 (2012),
https://hdl.handle.net/21.15107/rcub_rimsi_2574 .