TY  - JOUR
AU  - Todorović, Tamara
AU  - Vukašinović, Jelena
AU  - Portalone, Gustavo
AU  - suleiman, sherif
AU  - Gligorijević, Nevenka
AU  - Bjelogrlic, Snezana K.
AU  - Jovanovic, Katarina
AU  - Radulović, Sinisa S
AU  - Andjelkovic, Katarina
AU  - Cassar, Analisse
AU  - Filipović, Nenad
AU  - Schembri-WIsmayer, Pierre
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2027
AB  - Cobaltcomplexeswithsemi-andthiosemicarbazonesof8-quinolinecarboxaldehydehavebeensynthesizedandcharacterizedbyX-raydiffractionanalysis.Thesenovelcomplexesandapreviouslysynthesized cobaltcomplexwithaselenium-basedselenosemicarbazoneligandshowedmyeloiddifferentiationactivity onalltransretinoicacidresistantHL-60acutemyeloidleukaemiacells.Theyalsoshowedvaryinglevelsof cytotoxicityonfivehumantumorcelllines:cervixcarcinomacells(HeLa),lungadenocarcinomacells (A549),colorectaladenocarcinomacells(LS-174),breastcarcinomacells(MDA-MB-361),andchronicmyeloidleukaemia(K562)aswellasonenormalhumancellline:fetallungfibroblastcells(MRC-5).Leukaemia differentiationwasmoststronglyinducedbyametal-freeoxygenligandandtheseleniumligand,whilst thelatterandthecobaltIJII)complexwithanoxygenligandshowedthestrongestdose-dependentcytotoxic activity.Infouroutoffiveinvestigatedtumorcelllines,itwasofthesameorderofmagnitudeascisplatin. Thesebestcompounds,however,hadlowertoxicityonnon-transformedMRC-5cellsthancisplatin.
PB  - RSC Publishing
T2  - MedChemComm
T1  - (Chalcogen)semicarbazones and their cobalt complexes differentiate HL-60 myeloid leukaemia cells and are cytotoxic towards tumor cell lines
EP  - 111
IS  - 1
SP  - 103
VL  - 8
DO  - https://doi.org/10.1039/c6md00501b
ER  - 

@article{
author = "Todorović, Tamara and Vukašinović, Jelena and Portalone, Gustavo and suleiman, sherif and Gligorijević, Nevenka and Bjelogrlic, Snezana K. and Jovanovic, Katarina and Radulović, Sinisa S and Andjelkovic, Katarina and Cassar, Analisse and Filipović, Nenad and Schembri-WIsmayer, Pierre",
year = "2017",
abstract = "Cobaltcomplexeswithsemi-andthiosemicarbazonesof8-quinolinecarboxaldehydehavebeensynthesizedandcharacterizedbyX-raydiffractionanalysis.Thesenovelcomplexesandapreviouslysynthesized cobaltcomplexwithaselenium-basedselenosemicarbazoneligandshowedmyeloiddifferentiationactivity onalltransretinoicacidresistantHL-60acutemyeloidleukaemiacells.Theyalsoshowedvaryinglevelsof cytotoxicityonfivehumantumorcelllines:cervixcarcinomacells(HeLa),lungadenocarcinomacells (A549),colorectaladenocarcinomacells(LS-174),breastcarcinomacells(MDA-MB-361),andchronicmyeloidleukaemia(K562)aswellasonenormalhumancellline:fetallungfibroblastcells(MRC-5).Leukaemia differentiationwasmoststronglyinducedbyametal-freeoxygenligandandtheseleniumligand,whilst thelatterandthecobaltIJII)complexwithanoxygenligandshowedthestrongestdose-dependentcytotoxic activity.Infouroutoffiveinvestigatedtumorcelllines,itwasofthesameorderofmagnitudeascisplatin. Thesebestcompounds,however,hadlowertoxicityonnon-transformedMRC-5cellsthancisplatin.",
publisher = "RSC Publishing",
journal = "MedChemComm",
title = "(Chalcogen)semicarbazones and their cobalt complexes differentiate HL-60 myeloid leukaemia cells and are cytotoxic towards tumor cell lines",
pages = "111-103",
number = "1",
volume = "8",
doi = "https://doi.org/10.1039/c6md00501b"
}

Todorović, T., Vukašinović, J., Portalone, G., suleiman, s., Gligorijević, N., Bjelogrlic, S. K., Jovanovic, K., Radulović, S. S., Andjelkovic, K., Cassar, A., Filipović, N.,& Schembri-WIsmayer, P.. (2017). (Chalcogen)semicarbazones and their cobalt complexes differentiate HL-60 myeloid leukaemia cells and are cytotoxic towards tumor cell lines. in MedChemComm
RSC Publishing., 8(1), 103-111.
https://doi.org/https://doi.org/10.1039/c6md00501b

Todorović T, Vukašinović J, Portalone G, suleiman S, Gligorijević N, Bjelogrlic SK, Jovanovic K, Radulović SS, Andjelkovic K, Cassar A, Filipović N, Schembri-WIsmayer P. (Chalcogen)semicarbazones and their cobalt complexes differentiate HL-60 myeloid leukaemia cells and are cytotoxic towards tumor cell lines. in MedChemComm. 2017;8(1):103-111.
doi:https://doi.org/10.1039/c6md00501b .

Todorović, Tamara, Vukašinović, Jelena, Portalone, Gustavo, suleiman, sherif, Gligorijević, Nevenka, Bjelogrlic, Snezana K., Jovanovic, Katarina, Radulović, Sinisa S, Andjelkovic, Katarina, Cassar, Analisse, Filipović, Nenad, Schembri-WIsmayer, Pierre, "(Chalcogen)semicarbazones and their cobalt complexes differentiate HL-60 myeloid leukaemia cells and are cytotoxic towards tumor cell lines" in MedChemComm, 8, no. 1 (2017):103-111,
https://doi.org/https://doi.org/10.1039/c6md00501b . .

TY  - JOUR
AU  - Jovanović, Katarina K
AU  - Savić, Aleksandar G
AU  - Janković, Radmila N
AU  - Radulović, Sinisa S
AU  - Spasić, Slađana
AU  - Radotić, Ksenija
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/702
AB  - The key method for therapies of various cancer types could be the molecular-targeted therapy, based on individual gene profile for each patient. One of the main procedures used for genetic testing is the real-time polymerase chain reaction (real-time PCR). Physical principle behind real-time PCR procedure is the fluorescence. Fluorescence labeled probes (primers) is attached to quenchers. Upon reaction of polymerization, quenchers are removed, and the fluorescence emission intensity increases in time. Emission spectra shape and its maximum position can differ if the fluorophore was present in different microenvironment. That property is widely exploited in fluorescence spectroscopy and chromatography. This paper, for the first time, describes utilization of full spectroscopic potential of multichannel excitation/emission filter sets in real-time PCR device. Instead of monitoring fluorescence intensity in time for a single fluorescence emission channel, the ratio values of three different kinetics curves were calculated and analyzed by applying k-means clustering and dendrogram analysis. Obtained results have shown that described analytical improvement provides identification of nine different groups of mutations if the commercial QIAGEN (R) EGFR PCR Kit was used. Method can be applied to any kit, capable to simultaneously detect several different mutations.
PB  - Churchill Livingstone, Edinburgh
T2  - Medical Hypotheses
T1  - Detection of DNA mutations based on analysis of multiple wavelength excitation/emission fluorescence kinetics curves in real-time PCR
EP  - 379
IS  - 4
SP  - 376
VL  - 80
DO  - 10.1016/j.mehy.2013.01.004
ER  - 

@article{
author = "Jovanović, Katarina K and Savić, Aleksandar G and Janković, Radmila N and Radulović, Sinisa S and Spasić, Slađana and Radotić, Ksenija",
year = "2013",
abstract = "The key method for therapies of various cancer types could be the molecular-targeted therapy, based on individual gene profile for each patient. One of the main procedures used for genetic testing is the real-time polymerase chain reaction (real-time PCR). Physical principle behind real-time PCR procedure is the fluorescence. Fluorescence labeled probes (primers) is attached to quenchers. Upon reaction of polymerization, quenchers are removed, and the fluorescence emission intensity increases in time. Emission spectra shape and its maximum position can differ if the fluorophore was present in different microenvironment. That property is widely exploited in fluorescence spectroscopy and chromatography. This paper, for the first time, describes utilization of full spectroscopic potential of multichannel excitation/emission filter sets in real-time PCR device. Instead of monitoring fluorescence intensity in time for a single fluorescence emission channel, the ratio values of three different kinetics curves were calculated and analyzed by applying k-means clustering and dendrogram analysis. Obtained results have shown that described analytical improvement provides identification of nine different groups of mutations if the commercial QIAGEN (R) EGFR PCR Kit was used. Method can be applied to any kit, capable to simultaneously detect several different mutations.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "Medical Hypotheses",
title = "Detection of DNA mutations based on analysis of multiple wavelength excitation/emission fluorescence kinetics curves in real-time PCR",
pages = "379-376",
number = "4",
volume = "80",
doi = "10.1016/j.mehy.2013.01.004"
}

Jovanović, K. K., Savić, A. G., Janković, R. N., Radulović, S. S., Spasić, S.,& Radotić, K.. (2013). Detection of DNA mutations based on analysis of multiple wavelength excitation/emission fluorescence kinetics curves in real-time PCR. in Medical Hypotheses
Churchill Livingstone, Edinburgh., 80(4), 376-379.
https://doi.org/10.1016/j.mehy.2013.01.004

Jovanović KK, Savić AG, Janković RN, Radulović SS, Spasić S, Radotić K. Detection of DNA mutations based on analysis of multiple wavelength excitation/emission fluorescence kinetics curves in real-time PCR. in Medical Hypotheses. 2013;80(4):376-379.
doi:10.1016/j.mehy.2013.01.004 .

Jovanović, Katarina K, Savić, Aleksandar G, Janković, Radmila N, Radulović, Sinisa S, Spasić, Slađana, Radotić, Ksenija, "Detection of DNA mutations based on analysis of multiple wavelength excitation/emission fluorescence kinetics curves in real-time PCR" in Medical Hypotheses, 80, no. 4 (2013):376-379,
https://doi.org/10.1016/j.mehy.2013.01.004 . .