TY  - JOUR
AU  - Todorović, Sladana
AU  - Giba, Zlatko
AU  - Bačić, Goran
AU  - Nikolic, Miroslav
AU  - Grubisic, Dragoljub
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/299
AB  - The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environmental and Experimental Botany
T1  - High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds
EP  - 324
IS  - 3
SP  - 322
VL  - 64
DO  - 10.1016/j.envexpbot.2008.07.001
ER  - 

@article{
author = "Todorović, Sladana and Giba, Zlatko and Bačić, Goran and Nikolic, Miroslav and Grubisic, Dragoljub",
year = "2008",
abstract = "The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environmental and Experimental Botany",
title = "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds",
pages = "324-322",
number = "3",
volume = "64",
doi = "10.1016/j.envexpbot.2008.07.001"
}

Todorović, S., Giba, Z., Bačić, G., Nikolic, M.,& Grubisic, D.. (2008). High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany
Pergamon-Elsevier Science Ltd, Oxford., 64(3), 322-324.
https://doi.org/10.1016/j.envexpbot.2008.07.001

Todorović S, Giba Z, Bačić G, Nikolic M, Grubisic D. High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany. 2008;64(3):322-324.
doi:10.1016/j.envexpbot.2008.07.001 .

Todorović, Sladana, Giba, Zlatko, Bačić, Goran, Nikolic, Miroslav, Grubisic, Dragoljub, "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds" in Environmental and Experimental Botany, 64, no. 3 (2008):322-324,
https://doi.org/10.1016/j.envexpbot.2008.07.001 . .

TY  - JOUR
AU  - Misic, Danijela
AU  - Maksimović, Vuk
AU  - Todorović, S
AU  - Grubisic, D
AU  - Konjević, R
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/132
AB  - A wide range of sucrose, fructose, and glucose levels in culture media was tested in vitro in order to determine those that provide optimum growth, morphogenesis, and the production of secondary metabolites in Nepeta rtanjensis Diklic and Milojevic shoots. The effect of different concentrations of the carbohydrates in culture media on the internal carbohydrate status of N. rtanjensis shoots was also determined. Our results show that in vitro growth and development of N. rtanjensis, as well as nepetalactone accumulation, are significantly affected by both the type of carbohydrate and its concentration in the culture medium. Glucose proved to be the most efficient carbon and energy source.
PB  - Brill Academic Publishers, Leiden
T2  - Israel Journal of Plant Sciences
T1  - Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro
EP  - 108
IS  - 2
SP  - 103
VL  - 53
DO  - 10.1560/CBU6-G71U-TC65-FH1U
ER  - 

@article{
author = "Misic, Danijela and Maksimović, Vuk and Todorović, S and Grubisic, D and Konjević, R",
year = "2005",
abstract = "A wide range of sucrose, fructose, and glucose levels in culture media was tested in vitro in order to determine those that provide optimum growth, morphogenesis, and the production of secondary metabolites in Nepeta rtanjensis Diklic and Milojevic shoots. The effect of different concentrations of the carbohydrates in culture media on the internal carbohydrate status of N. rtanjensis shoots was also determined. Our results show that in vitro growth and development of N. rtanjensis, as well as nepetalactone accumulation, are significantly affected by both the type of carbohydrate and its concentration in the culture medium. Glucose proved to be the most efficient carbon and energy source.",
publisher = "Brill Academic Publishers, Leiden",
journal = "Israel Journal of Plant Sciences",
title = "Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro",
pages = "108-103",
number = "2",
volume = "53",
doi = "10.1560/CBU6-G71U-TC65-FH1U"
}

Misic, D., Maksimović, V., Todorović, S., Grubisic, D.,& Konjević, R.. (2005). Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro. in Israel Journal of Plant Sciences
Brill Academic Publishers, Leiden., 53(2), 103-108.
https://doi.org/10.1560/CBU6-G71U-TC65-FH1U

Misic D, Maksimović V, Todorović S, Grubisic D, Konjević R. Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro. in Israel Journal of Plant Sciences. 2005;53(2):103-108.
doi:10.1560/CBU6-G71U-TC65-FH1U .

Misic, Danijela, Maksimović, Vuk, Todorović, S, Grubisic, D, Konjević, R, "Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro" in Israel Journal of Plant Sciences, 53, no. 2 (2005):103-108,
https://doi.org/10.1560/CBU6-G71U-TC65-FH1U . .