TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Živanović, Branka  D.
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/266
AB  - Apoplastic fluid originated from maize (Zea mays L) leaf and root growing and non-growing regions were analyzed for peroxidase (POD) activities and phenols content. The samples were obtained by two different methods, as apoplastic washing fluid (AWF) from excised leaf and root segments, and as root apoplastic fluid (RAF) collected from the surface of intact roots by filter papers. AWF and RAF were tested for peroxidative and oxidative activity of POD, being lowest in the growing zones and gradually increasing toward root base and leaf tip. Isoelectric focusing of AWF and RAF revealed the differences pronounced in cationic group of peroxidases. Quantitative and qualitative analyzes of phenolic compounds from root and leaf apoplastic fluid was performed by HPLC. The increase of peroxidase activities was parallel to the increase of coniferyl alcohol, as well as other phenolic compound concentrations with root and leaf age, indicating that increased phenolic concentrations reflect increased lignin production during maturation. The data presented in this paper indicate that the method for getting RAF using filter papers is a more reliable procedure than infiltration/centrifugation method for studying processes which take place in root and leaf apoplastic fluid.
PB  - Elsevier Ireland Ltd, Clare
T2  - Plant Science
T1  - Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth
EP  - 662
IS  - 5
SP  - 656
VL  - 175
DO  - 10.1016/j.plantsci.2008.06.015
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Maksimović, Vuk and Živanović, Branka  D. and Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana",
year = "2008",
abstract = "Apoplastic fluid originated from maize (Zea mays L) leaf and root growing and non-growing regions were analyzed for peroxidase (POD) activities and phenols content. The samples were obtained by two different methods, as apoplastic washing fluid (AWF) from excised leaf and root segments, and as root apoplastic fluid (RAF) collected from the surface of intact roots by filter papers. AWF and RAF were tested for peroxidative and oxidative activity of POD, being lowest in the growing zones and gradually increasing toward root base and leaf tip. Isoelectric focusing of AWF and RAF revealed the differences pronounced in cationic group of peroxidases. Quantitative and qualitative analyzes of phenolic compounds from root and leaf apoplastic fluid was performed by HPLC. The increase of peroxidase activities was parallel to the increase of coniferyl alcohol, as well as other phenolic compound concentrations with root and leaf age, indicating that increased phenolic concentrations reflect increased lignin production during maturation. The data presented in this paper indicate that the method for getting RAF using filter papers is a more reliable procedure than infiltration/centrifugation method for studying processes which take place in root and leaf apoplastic fluid.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Plant Science",
title = "Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth",
pages = "662-656",
number = "5",
volume = "175",
doi = "10.1016/j.plantsci.2008.06.015"
}

Dragišić Maksimović, J., Maksimović, V., Živanović, Branka  D., Hadzi-Tasković Sukalović, V.,& Vuletic, M.. (2008). Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth. in Plant Science
Elsevier Ireland Ltd, Clare., 175(5), 656-662.
https://doi.org/10.1016/j.plantsci.2008.06.015

Dragišić Maksimović J, Maksimović V, Živanović, Branka  D., Hadzi-Tasković Sukalović V, Vuletic M. Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth. in Plant Science. 2008;175(5):656-662.
doi:10.1016/j.plantsci.2008.06.015 .

Dragišić Maksimović, Jelena, Maksimović, Vuk, Živanović, Branka  D., Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana, "Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth" in Plant Science, 175, no. 5 (2008):656-662,
https://doi.org/10.1016/j.plantsci.2008.06.015 . .

TY  - JOUR
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana
AU  - Vučinić, Željko
AU  - Veljović-Jovanović, Sonja
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/264
AB  - The rate of ascorbate and nicotinamide adenine dinucleotide plus hydrogen (NADH) cooxidation (i.e., their nonenzymic oxidation by peroxidase/H2O2-generated phenoxyl radicals of three hydroxycinnamates: caffeate, ferulate and p-coumarate) was studied in vitro. The reactions initiated by different sources of peroxidase (EC 1.11.1.7) [isolates from soybean (Glycine max L.) seed coat, maize (Zea mays L.) root-cell wall, and commercial horseradish peroxidase] were monitored. Native electrophoresis of samples and specific staining for peroxidase activity revealed various isoforms in each of the three enzyme sources. The peroxidase sources differed both in the rate of H2O2-dependent hydroxycinnamate oxidation and in the order of affinity for the phenolic substrates. The three hydroxycinnamates did not differ in their ability to cooxidize ascorbate, whereas NADH cooxidation was affected by substitution of the phenolic ring. Thus, p-coumarate was more efficient than caffeate in NADH cooxidation, with ferulate not being effective at all. Metal ions (Zn2+ and Al3+) inhibited the reaction of peroxidase with p-coumarate and affected the cooxidation rate of ascorbate and the peroxidase reaction in the same manner with all substrates used. However, inhibition of p-coumarate oxidation by metal ions did not affect NADH cooxidation rate. We propose that both the ascorbate and NADH cooxidation systems can function as mechanisms to scavenge H2O2 and regenerate phenolics in different cellular compartments, thus contributing to protection from oxidative damage.
PB  - Springer Japan Kk, Tokyo
T2  - Journal of Plant Research
T1  - Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH
EP  - 123
IS  - 1
SP  - 115
VL  - 121
DO  - 10.1007/s10265-007-0124-x
ER  - 

@article{
author = "Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana and Vučinić, Željko and Veljović-Jovanović, Sonja",
year = "2008",
abstract = "The rate of ascorbate and nicotinamide adenine dinucleotide plus hydrogen (NADH) cooxidation (i.e., their nonenzymic oxidation by peroxidase/H2O2-generated phenoxyl radicals of three hydroxycinnamates: caffeate, ferulate and p-coumarate) was studied in vitro. The reactions initiated by different sources of peroxidase (EC 1.11.1.7) [isolates from soybean (Glycine max L.) seed coat, maize (Zea mays L.) root-cell wall, and commercial horseradish peroxidase] were monitored. Native electrophoresis of samples and specific staining for peroxidase activity revealed various isoforms in each of the three enzyme sources. The peroxidase sources differed both in the rate of H2O2-dependent hydroxycinnamate oxidation and in the order of affinity for the phenolic substrates. The three hydroxycinnamates did not differ in their ability to cooxidize ascorbate, whereas NADH cooxidation was affected by substitution of the phenolic ring. Thus, p-coumarate was more efficient than caffeate in NADH cooxidation, with ferulate not being effective at all. Metal ions (Zn2+ and Al3+) inhibited the reaction of peroxidase with p-coumarate and affected the cooxidation rate of ascorbate and the peroxidase reaction in the same manner with all substrates used. However, inhibition of p-coumarate oxidation by metal ions did not affect NADH cooxidation rate. We propose that both the ascorbate and NADH cooxidation systems can function as mechanisms to scavenge H2O2 and regenerate phenolics in different cellular compartments, thus contributing to protection from oxidative damage.",
publisher = "Springer Japan Kk, Tokyo",
journal = "Journal of Plant Research",
title = "Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH",
pages = "123-115",
number = "1",
volume = "121",
doi = "10.1007/s10265-007-0124-x"
}

Hadzi-Tasković Sukalović, V., Vuletic, M., Vučinić, Ž.,& Veljović-Jovanović, S.. (2008). Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH. in Journal of Plant Research
Springer Japan Kk, Tokyo., 121(1), 115-123.
https://doi.org/10.1007/s10265-007-0124-x

Hadzi-Tasković Sukalović V, Vuletic M, Vučinić Ž, Veljović-Jovanović S. Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH. in Journal of Plant Research. 2008;121(1):115-123.
doi:10.1007/s10265-007-0124-x .

Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana, Vučinić, Željko, Veljović-Jovanović, Sonja, "Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH" in Journal of Plant Research, 121, no. 1 (2008):115-123,
https://doi.org/10.1007/s10265-007-0124-x . .