TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Oalđe Pavlović, Mariana
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Duletic-Lausevic, Sonja
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2180
AB  - BACKGROUND
According to World Health Organization, 70-95% of the population chooses folk medicine as their primary approach for health maintenance. In addition to their healing properties and application in folk medicine, Lamiaceae plants are often valued in cookery as spices and food preservatives.
OBJECTIVES
Hence, the aim of this study was to examine the antibiofilm activity of 12 ethanolic extracts of Lamiaceae species on biofilm formation and the degradation of existing biofilm of Pseudomonas aeruginosa PAO1 (ATCC 15692).
METHODS
The crystal violet staining method was used to evaluate the newly formed bacterial biofilms. Four concentrations (double dilutions) of plant extracts (starting conc. 2500 μg/mL), solvent control (ethanol, starting conc.15%), and positive control (streptomycin, starting conc. 12.5 μg/mL) were tested.
RESULTS
All ethanol extracts showed antibiofilm activity. However, the strongest activity was observed for Hyssopus officinalis, Melissa officinalis, Mentha piperita, and Ocimum basilicum, where the viability of bacteria in the biofilm after treatment was about 60% mainly at each tested concentration. Teucrium chamaedrys extract exhibited the strongest activity by degrading about 60% of biofilm (about 40% viability) at all concentrations except at the lowest tested one. Promising results were also observed for M. piperita (at each of the tested concentrations) and O. basilicum (at the highest tested concentration), where the viability of bacteria in the biofilm after treatment was reduced by about 40%. The remaining extracts showed a slightly lower effect on the degradation of the previously formed biofilm of P. aeruginosa PAO1.
PB  - Federation of European Materials Societies
C3  - FEMS Conference on Microbiology 2022, 30 June – 2 July 2022, Belgrade, Serbia.
T1  - Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?
SP  - 347
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2180
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Oalđe Pavlović, Mariana and Jovanović Marić, Jovana and Kolarević, Stoimir and Duletic-Lausevic, Sonja and Vuković-Gačić, Branka",
year = "2022",
abstract = "BACKGROUND
According to World Health Organization, 70-95% of the population chooses folk medicine as their primary approach for health maintenance. In addition to their healing properties and application in folk medicine, Lamiaceae plants are often valued in cookery as spices and food preservatives.
OBJECTIVES
Hence, the aim of this study was to examine the antibiofilm activity of 12 ethanolic extracts of Lamiaceae species on biofilm formation and the degradation of existing biofilm of Pseudomonas aeruginosa PAO1 (ATCC 15692).
METHODS
The crystal violet staining method was used to evaluate the newly formed bacterial biofilms. Four concentrations (double dilutions) of plant extracts (starting conc. 2500 μg/mL), solvent control (ethanol, starting conc.15%), and positive control (streptomycin, starting conc. 12.5 μg/mL) were tested.
RESULTS
All ethanol extracts showed antibiofilm activity. However, the strongest activity was observed for Hyssopus officinalis, Melissa officinalis, Mentha piperita, and Ocimum basilicum, where the viability of bacteria in the biofilm after treatment was about 60% mainly at each tested concentration. Teucrium chamaedrys extract exhibited the strongest activity by degrading about 60% of biofilm (about 40% viability) at all concentrations except at the lowest tested one. Promising results were also observed for M. piperita (at each of the tested concentrations) and O. basilicum (at the highest tested concentration), where the viability of bacteria in the biofilm after treatment was reduced by about 40%. The remaining extracts showed a slightly lower effect on the degradation of the previously formed biofilm of P. aeruginosa PAO1.",
publisher = "Federation of European Materials Societies",
journal = "FEMS Conference on Microbiology 2022, 30 June – 2 July 2022, Belgrade, Serbia.",
title = "Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?",
pages = "347",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2180"
}

Đorđević Aleksić, J., Oalđe Pavlović, M., Jovanović Marić, J., Kolarević, S., Duletic-Lausevic, S.,& Vuković-Gačić, B.. (2022). Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?. in FEMS Conference on Microbiology 2022, 30 June – 2 July 2022, Belgrade, Serbia.
Federation of European Materials Societies., 347.
https://hdl.handle.net/21.15107/rcub_rimsi_2180

Đorđević Aleksić J, Oalđe Pavlović M, Jovanović Marić J, Kolarević S, Duletic-Lausevic S, Vuković-Gačić B. Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?. in FEMS Conference on Microbiology 2022, 30 June – 2 July 2022, Belgrade, Serbia.. 2022;:347.
https://hdl.handle.net/21.15107/rcub_rimsi_2180 .

Đorđević Aleksić, Jelena, Oalđe Pavlović, Mariana, Jovanović Marić, Jovana, Kolarević, Stoimir, Duletic-Lausevic, Sonja, Vuković-Gačić, Branka, "Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?" in FEMS Conference on Microbiology 2022, 30 June – 2 July 2022, Belgrade, Serbia. (2022):347,
https://hdl.handle.net/21.15107/rcub_rimsi_2180 .