TY  - CONF
AU  - Kalicanin, Nevena
AU  - Balaz, Ana Marija
AU  - Prodanović, Olivera
AU  - Prodanović, Radivoje
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3048
AB  - Opine dehydrogenases are a family of NAD(P)H dependent oxidoreductases, which
catalyze the reductive condensation of an α amino group from an amino acid with an αketo acid during anaerobic glycolysis by regenerating NAD. They are widespread in
cephalopods and mollusks. Opines are associated with crown gall tumor pathogenesis
caused by A. tumefaciens providing nutrients to the pathogen, and novel opine compounds
acting as metallophores have been identified. Besides, opine-type secondary amine
dicarboxylic acids are chiral intermediates of angiotensin-converting enzyme inhibitors. A
novel enzyme originating from an extremophile bacterium, with assumed opine
dehydrogenase function was successfully expressed in Escherichia coli STAR cells and
purified by affinity chromatography. Molecular mass determined by SDS-PAGE was
approximately 40 kDa. The activity was measured by using pyruvate and alanine as
substrates, by which proved that it has opine dehydrogenase activity.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Proceedings Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry"
T1  - Production of a novel opine dehydrogenase
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3048
ER  - 

@conference{
author = "Kalicanin, Nevena and Balaz, Ana Marija and Prodanović, Olivera and Prodanović, Radivoje",
year = "2022",
abstract = "Opine dehydrogenases are a family of NAD(P)H dependent oxidoreductases, which
catalyze the reductive condensation of an α amino group from an amino acid with an αketo acid during anaerobic glycolysis by regenerating NAD. They are widespread in
cephalopods and mollusks. Opines are associated with crown gall tumor pathogenesis
caused by A. tumefaciens providing nutrients to the pathogen, and novel opine compounds
acting as metallophores have been identified. Besides, opine-type secondary amine
dicarboxylic acids are chiral intermediates of angiotensin-converting enzyme inhibitors. A
novel enzyme originating from an extremophile bacterium, with assumed opine
dehydrogenase function was successfully expressed in Escherichia coli STAR cells and
purified by affinity chromatography. Molecular mass determined by SDS-PAGE was
approximately 40 kDa. The activity was measured by using pyruvate and alanine as
substrates, by which proved that it has opine dehydrogenase activity.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Proceedings Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry"",
title = "Production of a novel opine dehydrogenase",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3048"
}

Kalicanin, N., Balaz, A. M., Prodanović, O.,& Prodanović, R.. (2022). Production of a novel opine dehydrogenase. in Proceedings Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry"
Faculty of Chemistry, Serbian Biochemical Society., 79.
https://hdl.handle.net/21.15107/rcub_rimsi_3048

Kalicanin N, Balaz AM, Prodanović O, Prodanović R. Production of a novel opine dehydrogenase. in Proceedings Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry". 2022;:79.
https://hdl.handle.net/21.15107/rcub_rimsi_3048 .

Kalicanin, Nevena, Balaz, Ana Marija, Prodanović, Olivera, Prodanović, Radivoje, "Production of a novel opine dehydrogenase" in Proceedings Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry" (2022):79,
https://hdl.handle.net/21.15107/rcub_rimsi_3048 .