TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Kracun, Margareta
AU  - Vasiljević, Bozica
AU  - Tomović, Jelena M
AU  - Vukovic-Gacic, Branka
AU  - Gačić, Zoran
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/703
AB  - This study was undertaken to investigate the potential of the freshwater mussel Sinanodonta woodiana for detection of genotoxic pollution of the environment. Study was performed at two sites in the Velika Morava River, from May 2010 to February 2011. The alkaline comet assay on haemocytes was used, and the olive tail moment (OTM) was chosen as a measure of DNA damage. The specimens held on acclimation under controlled laboratory conditions for 10 d were used as a control. Chemical analysis revealed the presence of phosphates and increased concentrations of zinc, copper and nickel at both sites during the entire sampling period. The values of OTM in mussels collected from the environment, significantly correlated with the concentration of zinc (r = 0.6248), temperature (r = 0.7006) and dissolved oxygen (r = 0.7738). Seasonal variations in genotoxic response were observed, with the highest OTM values obtained during summer months. Preliminary results of the in vitro study indicated the effect of water temperature on genotoxic response to zinc and cadmium in S. woodiana suggesting that the presence of genotoxic pollutants during months with lower temperature could be under-estimated. Obtained results indicate that S. woodiana could be a valuable tool for active biomonitoring of aquatic environments and emphasizes the importance of seasonal genotoxic monitoring with this organism.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay
EP  - 251
IS  - 2
SP  - 243
VL  - 93
DO  - 10.1016/j.chemosphere.2013.04.073
ER  - 

@article{
author = "Kolarević, Stoimir and Knezevic-Vukcević, Jelena and Paunović, Momir and Kracun, Margareta and Vasiljević, Bozica and Tomović, Jelena M and Vukovic-Gacic, Branka and Gačić, Zoran",
year = "2013",
abstract = "This study was undertaken to investigate the potential of the freshwater mussel Sinanodonta woodiana for detection of genotoxic pollution of the environment. Study was performed at two sites in the Velika Morava River, from May 2010 to February 2011. The alkaline comet assay on haemocytes was used, and the olive tail moment (OTM) was chosen as a measure of DNA damage. The specimens held on acclimation under controlled laboratory conditions for 10 d were used as a control. Chemical analysis revealed the presence of phosphates and increased concentrations of zinc, copper and nickel at both sites during the entire sampling period. The values of OTM in mussels collected from the environment, significantly correlated with the concentration of zinc (r = 0.6248), temperature (r = 0.7006) and dissolved oxygen (r = 0.7738). Seasonal variations in genotoxic response were observed, with the highest OTM values obtained during summer months. Preliminary results of the in vitro study indicated the effect of water temperature on genotoxic response to zinc and cadmium in S. woodiana suggesting that the presence of genotoxic pollutants during months with lower temperature could be under-estimated. Obtained results indicate that S. woodiana could be a valuable tool for active biomonitoring of aquatic environments and emphasizes the importance of seasonal genotoxic monitoring with this organism.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay",
pages = "251-243",
number = "2",
volume = "93",
doi = "10.1016/j.chemosphere.2013.04.073"
}

Kolarević, S., Knezevic-Vukcević, J., Paunović, M., Kracun, M., Vasiljević, B., Tomović, J. M., Vukovic-Gacic, B.,& Gačić, Z.. (2013). Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay. in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 93(2), 243-251.
https://doi.org/10.1016/j.chemosphere.2013.04.073

Kolarević S, Knezevic-Vukcević J, Paunović M, Kracun M, Vasiljević B, Tomović JM, Vukovic-Gacic B, Gačić Z. Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay. in Chemosphere. 2013;93(2):243-251.
doi:10.1016/j.chemosphere.2013.04.073 .

Kolarević, Stoimir, Knezevic-Vukcević, Jelena, Paunović, Momir, Kracun, Margareta, Vasiljević, Bozica, Tomović, Jelena M, Vukovic-Gacic, Branka, Gačić, Zoran, "Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay" in Chemosphere, 93, no. 2 (2013):243-251,
https://doi.org/10.1016/j.chemosphere.2013.04.073 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Vasiljević, Bozica
AU  - Kracun, Margareta
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/526
AB  - In this study, we investigated the level of sanitary pollution and organic contamination of the Velika Morava River, the largest river in central Serbia. Samples of water for microbiological analysis were collected at 5 sites, monthly, from April 2010 to February 2011. Sanitary analysis, i.e. enumeration of total and fecal coliforms and intestinal enterococci, indicated moderate to critical fecal contamination, while organic load assessment (oligotroph to heterotroph ratio, index of phosphatase activity) revealed the category of moderately polluted water. We also investigated seasonal variations of these groups of bacteria and the factors that could contribute to these differences. Our results showed that the microbiological quality of the water in the Velika Morava River during different seasons is affected by numerous factors such as unequal loading of wastewaters, solar irradiation, and relations of flow/dilution and rainfall/runoff.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Seasonal variations of microbiological parameters of water quality of the Velika Morava river Serbia
EP  - 1027
IS  - 3
SP  - 1017
VL  - 64
DO  - 10.2298/ABS1203017K
ER  - 

@article{
author = "Kolarević, Stoimir and Knezevic-Vukcević, Jelena and Paunović, Momir and Vasiljević, Bozica and Kracun, Margareta and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2012",
abstract = "In this study, we investigated the level of sanitary pollution and organic contamination of the Velika Morava River, the largest river in central Serbia. Samples of water for microbiological analysis were collected at 5 sites, monthly, from April 2010 to February 2011. Sanitary analysis, i.e. enumeration of total and fecal coliforms and intestinal enterococci, indicated moderate to critical fecal contamination, while organic load assessment (oligotroph to heterotroph ratio, index of phosphatase activity) revealed the category of moderately polluted water. We also investigated seasonal variations of these groups of bacteria and the factors that could contribute to these differences. Our results showed that the microbiological quality of the water in the Velika Morava River during different seasons is affected by numerous factors such as unequal loading of wastewaters, solar irradiation, and relations of flow/dilution and rainfall/runoff.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Seasonal variations of microbiological parameters of water quality of the Velika Morava river Serbia",
pages = "1027-1017",
number = "3",
volume = "64",
doi = "10.2298/ABS1203017K"
}

Kolarević, S., Knezevic-Vukcević, J., Paunović, M., Vasiljević, B., Kracun, M., Gačić, Z.,& Vukovic-Gacic, B.. (2012). Seasonal variations of microbiological parameters of water quality of the Velika Morava river Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(3), 1017-1027.
https://doi.org/10.2298/ABS1203017K

Kolarević S, Knezevic-Vukcević J, Paunović M, Vasiljević B, Kracun M, Gačić Z, Vukovic-Gacic B. Seasonal variations of microbiological parameters of water quality of the Velika Morava river Serbia. in Archives of Biological Sciences. 2012;64(3):1017-1027.
doi:10.2298/ABS1203017K .

Kolarević, Stoimir, Knezevic-Vukcević, Jelena, Paunović, Momir, Vasiljević, Bozica, Kracun, Margareta, Gačić, Zoran, Vukovic-Gacic, Branka, "Seasonal variations of microbiological parameters of water quality of the Velika Morava river Serbia" in Archives of Biological Sciences, 64, no. 3 (2012):1017-1027,
https://doi.org/10.2298/ABS1203017K . .