TY  - JOUR
AU  - Savic, Jasna
AU  - Pavlović, Jelena
AU  - Stanojević, Miloš
AU  - Bosnić, Predrag
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolić, Nina
AU  - Nikolic, Miroslav
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1911
AB  - Monocots and dicots differ in their boron (B) requirement, but also in their capacity to accumulate silicon (Si). Although an ameliorative effect of Si on B toxicity has been reported in various crops, differences among monocots and dicots are not clear, in particular in light of their ability to retain B in the leaf apoplast. In hydroponic experiments under controlled conditions, we studied the role of Si in the compartmentation of B within the leaves of wheat (Triticum vulgare L.) as a model of a high-Si monocot and sunflower (Helianthus annuus L.) as a model of a low-Si dicot, with the focus on the leaf apoplast. The stable isotopes 10B and 11B were used to investigate the dynamics of cell wall B binding capacity. In both crops, the application of Si did not affect B concentration in the root, but significantly decreased the B concentration in the leaves. However, the application of Si differently influenced the binding capacity of the leaf apoplast for excess B in wheat and sunflower. In wheat, whose capacity to retain B in the leaf cell walls is lower than in sunflower, the continuous supply of Si is crucial for an enhancement of high B tolerance in the shoot. On the other hand, the supply of Si did not contribute significantly in the extension of the B binding sites in sunflower leaves.
PB  - MDPI
T2  - Plants
T1  - Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves
SP  - 1660
VL  - 12
DO  - 10.3390/ plants12081660
ER  - 

@article{
author = "Savic, Jasna and Pavlović, Jelena and Stanojević, Miloš and Bosnić, Predrag and Kostić Kravljanac, Ljiljana and Nikolić, Nina and Nikolic, Miroslav",
year = "2023",
abstract = "Monocots and dicots differ in their boron (B) requirement, but also in their capacity to accumulate silicon (Si). Although an ameliorative effect of Si on B toxicity has been reported in various crops, differences among monocots and dicots are not clear, in particular in light of their ability to retain B in the leaf apoplast. In hydroponic experiments under controlled conditions, we studied the role of Si in the compartmentation of B within the leaves of wheat (Triticum vulgare L.) as a model of a high-Si monocot and sunflower (Helianthus annuus L.) as a model of a low-Si dicot, with the focus on the leaf apoplast. The stable isotopes 10B and 11B were used to investigate the dynamics of cell wall B binding capacity. In both crops, the application of Si did not affect B concentration in the root, but significantly decreased the B concentration in the leaves. However, the application of Si differently influenced the binding capacity of the leaf apoplast for excess B in wheat and sunflower. In wheat, whose capacity to retain B in the leaf cell walls is lower than in sunflower, the continuous supply of Si is crucial for an enhancement of high B tolerance in the shoot. On the other hand, the supply of Si did not contribute significantly in the extension of the B binding sites in sunflower leaves.",
publisher = "MDPI",
journal = "Plants",
title = "Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves",
pages = "1660",
volume = "12",
doi = "10.3390/ plants12081660"
}

Savic, J., Pavlović, J., Stanojević, M., Bosnić, P., Kostić Kravljanac, L., Nikolić, N.,& Nikolic, M.. (2023). Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves. in Plants
MDPI., 12, 1660.
https://doi.org/10.3390/ plants12081660

Savic J, Pavlović J, Stanojević M, Bosnić P, Kostić Kravljanac L, Nikolić N, Nikolic M. Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves. in Plants. 2023;12:1660.
doi:10.3390/ plants12081660 .

Savic, Jasna, Pavlović, Jelena, Stanojević, Miloš, Bosnić, Predrag, Kostić Kravljanac, Ljiljana, Nikolić, Nina, Nikolic, Miroslav, "Silicon Differently Affects Apoplastic Binding of Excess Boron in Wheat and Sunflower Leaves" in Plants, 12 (2023):1660,
https://doi.org/10.3390/ plants12081660 . .

TY  - CONF
AU  - Trailović, Maja
AU  - Kostić Kravljanac, Ljiljana
AU  - Stanojević, Miloš
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
AU  - TODIC, Slavica
AU  - Nikolic, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2140
AB  - Silicon (Si) uptake by crops is well studied and Si transporters have been characterized in various crop species, including grapevine. However, information on the rhizosphere mobilization of Si is still lacking and virtually no information is available on grapevine. Our previous study showed that grapevine is a phosphorus (P)-efficient species with a high root capacity to mobilize P from the rhizosphere by the released of organic anions (mainly citrate). The field experiment was established in 12-y-old vineyard with the cultivar ‘Chardonnay’, grafted on 5BB rootstock under extremely low P conditions (Olsen P < 3 mg kg-1). Four own-designed rhizotrons (80 cm depth) were installed in a vineyard enabling easy access to the new intact roots. The following treatments were performed: –P/–Si, +P/–Si, –P/+Si (soil application) and –P/+Si (foliar application). The samples of rhizosphere and bulk soils, root exudates from intact root tips and vine tissues (root and leaves) were collected at different growth stages according to Eichhorn-Lorentz (E-L) system: flowering (E-L stage 23), berries pea-size (E-L stage 31), and veraison (E-L stage 35). In addition to Si and P concentrations in the tissues, the expressions of VvALMT, VvMATE (encoding efflux transporters for malate and citrate, respectively), and VvNIP2.1 (encoding Si influx transporter) were also determined. Phosphate fertilization decreased, while low soil P and Si fertilization increased Si availability in the rhizosphere. At the flowering stage, –P plants accumulated more Si than the P-fertilized ones and was comparable to the Si-fertilized plants. Foliar application of Si was less effective in comparison with soil application unless at the veraison stage. The leaf Si concentrations showed a clear seasonal pattern being the highest at the veraison stage. Exudation rate of citrate also showed a clear seasonal pattern and was significantly higher in the –P/–Si than in +P/–Si plants, which was followed by an increased Si availability in the vine rhizosphere. Overall, low P conditions induced Si accumulation in the leaves due to increased exudation of organic anions that can also mobilize Si in the rhizosphere, thereby increasing Si uptake by grapevine.
PB  - ISSAG and AgCenter LSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2140
ER  - 

@conference{
author = "Trailović, Maja and Kostić Kravljanac, Ljiljana and Stanojević, Miloš and Pavlović, Jelena and Bosnić, Predrag and TODIC, Slavica and Nikolic, Miroslav",
year = "2022",
abstract = "Silicon (Si) uptake by crops is well studied and Si transporters have been characterized in various crop species, including grapevine. However, information on the rhizosphere mobilization of Si is still lacking and virtually no information is available on grapevine. Our previous study showed that grapevine is a phosphorus (P)-efficient species with a high root capacity to mobilize P from the rhizosphere by the released of organic anions (mainly citrate). The field experiment was established in 12-y-old vineyard with the cultivar ‘Chardonnay’, grafted on 5BB rootstock under extremely low P conditions (Olsen P < 3 mg kg-1). Four own-designed rhizotrons (80 cm depth) were installed in a vineyard enabling easy access to the new intact roots. The following treatments were performed: –P/–Si, +P/–Si, –P/+Si (soil application) and –P/+Si (foliar application). The samples of rhizosphere and bulk soils, root exudates from intact root tips and vine tissues (root and leaves) were collected at different growth stages according to Eichhorn-Lorentz (E-L) system: flowering (E-L stage 23), berries pea-size (E-L stage 31), and veraison (E-L stage 35). In addition to Si and P concentrations in the tissues, the expressions of VvALMT, VvMATE (encoding efflux transporters for malate and citrate, respectively), and VvNIP2.1 (encoding Si influx transporter) were also determined. Phosphate fertilization decreased, while low soil P and Si fertilization increased Si availability in the rhizosphere. At the flowering stage, –P plants accumulated more Si than the P-fertilized ones and was comparable to the Si-fertilized plants. Foliar application of Si was less effective in comparison with soil application unless at the veraison stage. The leaf Si concentrations showed a clear seasonal pattern being the highest at the veraison stage. Exudation rate of citrate also showed a clear seasonal pattern and was significantly higher in the –P/–Si than in +P/–Si plants, which was followed by an increased Si availability in the vine rhizosphere. Overall, low P conditions induced Si accumulation in the leaves due to increased exudation of organic anions that can also mobilize Si in the rhizosphere, thereby increasing Si uptake by grapevine.",
publisher = "ISSAG and AgCenter LSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2140"
}

Trailović, M., Kostić Kravljanac, L., Stanojević, M., Pavlović, J., Bosnić, P., TODIC, S.,& Nikolic, M.. (2022). Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenter LSU..
https://hdl.handle.net/21.15107/rcub_rimsi_2140

Trailović M, Kostić Kravljanac L, Stanojević M, Pavlović J, Bosnić P, TODIC S, Nikolic M. Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_2140 .

Trailović, Maja, Kostić Kravljanac, Ljiljana, Stanojević, Miloš, Pavlović, Jelena, Bosnić, Predrag, TODIC, Slavica, Nikolic, Miroslav, "Phosphorus Deficiency Induced Silicon Mobilization in Grapevine Rhizosphere: A Field Study" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2140 .

TY  - CONF
AU  - Carballo Méndez, Fernand
AU  - Bosnić, Predrag
AU  - Bosnic, Dragana
AU  - Nikolić, Nina
AU  - Kostić Kravljanac, Ljiljana
AU  - Stanojević, Miloš
AU  - Nikolic, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2120
AB  - Priming with silicon (Si) may increase plant resistance to biotic and abiotic stresses, in particular in conjunction with its subsequent application. Yet, the very effect of the duration of priming with Si is less understood. Here, we investigated the effect of the duration of priming with Si on components of the antioxidative response of wheat exposed to a gradient of salinity stress. After priming with 1.5 mM Si(OH)4 (0, 1, and 3 days), wheat seedlings were exposed to different NaCl levels (0, 30, and 60 mM) without (-Si) or with (+Si) supply of 1.5 mM Si(OH)4. The activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), and the concentration of malondialdehyde (MDA) were measured in shoots and roots after 1 and 5 days of NaCl treatments. Interaction of priming duration and addition of Si on antioxidative variables were analyzed using general regression model. Overall, priming had no influence on -Si plants under salt stress. On the day 1 of NaCl exposure, priming duration did not affect SOD and APX activities neither in roots nor in shoots. However, on the day 5 of NaCl exposure longer priming with Si significantly increased the activities of APX and SOD in both roots and shoots. The activity of CAT showed no response to priming with Si and subsequent Si supply in both plant organs irrespectively of the duration of NaCl exposure. Interestingly, in both organs the concentrations of MDA as a proxy for oxidative damage of plant membranes were very clearly and consistently lower after 3 days of priming with Si (compared to 1 day or no priming) during the whole period of NaCl exposure. This study demonstrated that longer priming with Si can enhance the ameliorative effect of Si supply on the antioxidative response of wheat plants to a gradient of salinity stress.
PB  - ISSAG and AgCenter LSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Duration of priming with silicon modulates antioxidative response of wheat to salinity stress
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2120
ER  - 

@conference{
author = "Carballo Méndez, Fernand and Bosnić, Predrag and Bosnic, Dragana and Nikolić, Nina and Kostić Kravljanac, Ljiljana and Stanojević, Miloš and Nikolic, Miroslav",
year = "2022",
abstract = "Priming with silicon (Si) may increase plant resistance to biotic and abiotic stresses, in particular in conjunction with its subsequent application. Yet, the very effect of the duration of priming with Si is less understood. Here, we investigated the effect of the duration of priming with Si on components of the antioxidative response of wheat exposed to a gradient of salinity stress. After priming with 1.5 mM Si(OH)4 (0, 1, and 3 days), wheat seedlings were exposed to different NaCl levels (0, 30, and 60 mM) without (-Si) or with (+Si) supply of 1.5 mM Si(OH)4. The activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), and the concentration of malondialdehyde (MDA) were measured in shoots and roots after 1 and 5 days of NaCl treatments. Interaction of priming duration and addition of Si on antioxidative variables were analyzed using general regression model. Overall, priming had no influence on -Si plants under salt stress. On the day 1 of NaCl exposure, priming duration did not affect SOD and APX activities neither in roots nor in shoots. However, on the day 5 of NaCl exposure longer priming with Si significantly increased the activities of APX and SOD in both roots and shoots. The activity of CAT showed no response to priming with Si and subsequent Si supply in both plant organs irrespectively of the duration of NaCl exposure. Interestingly, in both organs the concentrations of MDA as a proxy for oxidative damage of plant membranes were very clearly and consistently lower after 3 days of priming with Si (compared to 1 day or no priming) during the whole period of NaCl exposure. This study demonstrated that longer priming with Si can enhance the ameliorative effect of Si supply on the antioxidative response of wheat plants to a gradient of salinity stress.",
publisher = "ISSAG and AgCenter LSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Duration of priming with silicon modulates antioxidative response of wheat to salinity stress",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2120"
}

Carballo Méndez, F., Bosnić, P., Bosnic, D., Nikolić, N., Kostić Kravljanac, L., Stanojević, M.,& Nikolic, M.. (2022). Duration of priming with silicon modulates antioxidative response of wheat to salinity stress. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenter LSU..
https://hdl.handle.net/21.15107/rcub_rimsi_2120

Carballo Méndez F, Bosnić P, Bosnic D, Nikolić N, Kostić Kravljanac L, Stanojević M, Nikolic M. Duration of priming with silicon modulates antioxidative response of wheat to salinity stress. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_2120 .

Carballo Méndez, Fernand, Bosnić, Predrag, Bosnic, Dragana, Nikolić, Nina, Kostić Kravljanac, Ljiljana, Stanojević, Miloš, Nikolic, Miroslav, "Duration of priming with silicon modulates antioxidative response of wheat to salinity stress" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2120 .

TY  - CONF
AU  - Kostić Kravljanac, Ljiljana
AU  - Trailović, Maja
AU  - Pavlović, Jelena
AU  - Kostić, Igor
AU  - Dubljanin, Tijana
AU  - Nikolic, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2135
AB  - Silicon (Si) is the major constituent of soil present in various fractions, i.e., mobile, adsorbed, occluded (in pedogenic oxides and hydroxides), amorphous (biogenic and lithogenic) and crystalline (primary and secondary silicates, and quartz). Different soil factors such as pH, temperatures, microbial activity, the presence of cations, Al/Fe oxides and hydroxides and organic compounds, influence Si transformation, thereby modifying plant availably of Si. Silicon mobility and transformation in the soil have mainly been studied in the context of pedogenesis or biogeochemical Si cycling. However, research on Si mobility, transformation, and plant availability in the rhizosphere is still lacking. Here, we investigated the root potential of white lupine (Lupinus albus L.), known as a phosphorus (P)-efficient model plant (e.g., root release of H+ and carboxylates), to mobilize Si from the soil. Plants were grown in the rhizoboxes filled with low P soil (control) and fertilized with different N-forms (NO3, NH4 and NO3NH4). The control, NO3- and NO3NH4-fertilized plants accumulated significantly lower amounts of Si than the NH4-fertilized ones. All applied N-forms influenced Si availability in the bulk soil, but Si fractions have further been modified in the rhizosphere, what was crucial for Si accumulation in plants. For instance, NO3 supply slightly decreased Si availability in the bulk soil, but lupine plants accumulated a similar amount of Si as the control plants. A strong gradient of decreasing Si concentrations between bulk and rhizosphere soils was observed in mobile, adsorbed, and amorphous biogenic Si pools in the control and in all N treatments, while occluded and lithogenic amorphous Si pools were recalcitrant. Interestingly, a gradient of increasing concentrations of the amorphous biogenic Si pool between bulk and rhizosphere soils was recorded in the NH4 treatment, concomitantly with the strongest rhizosphere acidification.
PB  - ISSAG and AgCenter LSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2135
ER  - 

@conference{
author = "Kostić Kravljanac, Ljiljana and Trailović, Maja and Pavlović, Jelena and Kostić, Igor and Dubljanin, Tijana and Nikolic, Miroslav",
year = "2022",
abstract = "Silicon (Si) is the major constituent of soil present in various fractions, i.e., mobile, adsorbed, occluded (in pedogenic oxides and hydroxides), amorphous (biogenic and lithogenic) and crystalline (primary and secondary silicates, and quartz). Different soil factors such as pH, temperatures, microbial activity, the presence of cations, Al/Fe oxides and hydroxides and organic compounds, influence Si transformation, thereby modifying plant availably of Si. Silicon mobility and transformation in the soil have mainly been studied in the context of pedogenesis or biogeochemical Si cycling. However, research on Si mobility, transformation, and plant availability in the rhizosphere is still lacking. Here, we investigated the root potential of white lupine (Lupinus albus L.), known as a phosphorus (P)-efficient model plant (e.g., root release of H+ and carboxylates), to mobilize Si from the soil. Plants were grown in the rhizoboxes filled with low P soil (control) and fertilized with different N-forms (NO3, NH4 and NO3NH4). The control, NO3- and NO3NH4-fertilized plants accumulated significantly lower amounts of Si than the NH4-fertilized ones. All applied N-forms influenced Si availability in the bulk soil, but Si fractions have further been modified in the rhizosphere, what was crucial for Si accumulation in plants. For instance, NO3 supply slightly decreased Si availability in the bulk soil, but lupine plants accumulated a similar amount of Si as the control plants. A strong gradient of decreasing Si concentrations between bulk and rhizosphere soils was observed in mobile, adsorbed, and amorphous biogenic Si pools in the control and in all N treatments, while occluded and lithogenic amorphous Si pools were recalcitrant. Interestingly, a gradient of increasing concentrations of the amorphous biogenic Si pool between bulk and rhizosphere soils was recorded in the NH4 treatment, concomitantly with the strongest rhizosphere acidification.",
publisher = "ISSAG and AgCenter LSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2135"
}

Kostić Kravljanac, L., Trailović, M., Pavlović, J., Kostić, I., Dubljanin, T.,& Nikolic, M.. (2022). Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenter LSU..
https://hdl.handle.net/21.15107/rcub_rimsi_2135

Kostić Kravljanac L, Trailović M, Pavlović J, Kostić I, Dubljanin T, Nikolic M. Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_2135 .

Kostić Kravljanac, Ljiljana, Trailović, Maja, Pavlović, Jelena, Kostić, Igor, Dubljanin, Tijana, Nikolic, Miroslav, "Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2135 .

TY  - CONF
AU  - Kostić, Igor
AU  - Milenkovic, Ivan
AU  - Nikolić, Nina
AU  - Milanovic, Slobodan
AU  - Kostić Kravljanac, Ljiljana
AU  - Bosnić, Predrag
AU  - Paravinja, Ana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2112
AB  - Pedunculate oak (Quercus robur L.) is the most abundant deciduous tree species in Europe with high economical and ecological importance. Different species of Phytophthora are considered as one of the most important factors responsible for deterioration of oak forest, causing serious root damage not only in the forest trees, but also in the nurseries. Oak seedlings were grown in plastic pots with extremely low phosphorus (P) soil (1.5 mg kg-1 total P; no available Olsen-P detected). Silicon (Si) and P were supplied as Na2SiO3 (300 mg Si kg-1 dry soil) and KH2PO4 (180 mg P kg-1 dry soil), respectively. Four treatments (-P/-Si, -P+/Si, +P/-Si, and +P/+Si) were used in the experiment. After two months of experiment, a half of the plants in each treatment were root-inoculated with Phytophthora plurivora. After further four weeks, the first symptoms of P. plurivora infection appeared in leaves (e.g., leaf necrosis and wilting). Plants were then carefully removed from the pots, divided into roots and shoots, and the roots were scanned and analyzed by the WinRHIZO® software. Foliar concentrations of Si, P, K, Ca, Mg, B, Cu, Fe, Mn, and Zn were determined by ICP-OES, while the concentrations of N and S were determined by CHNS Analyzer. The addition of Si obviously improved root health status (e.g., decreasing de number of lesions and necrosis intensity) in the infected plants grown under -P conditions, which was followed by an increased foliar P concentration. The Si supply significantly increased the root variables (e.g., total root volume, root length, and area of thin roots) in both -P and +P plants inoculated with P. plurivora. Therefore, P. plurivora infection and supply of P and Si modulated the nutrient uptake and thereby changed the leaf ionomics, especially for infected -P plants supplied with Si (e.g., significantly increased B, Cu, and Si foliar concentrations and decreased Fe, Mn, Ca, Mg, K, and S foliar concentrations). Furthermore, Si fertilization significantly declined loses in plant dry biomass caused by P. plurivora infection and/or P deficiency, showing biomass comparable to non-infected +P plants.
PB  - ISSAG and AgCenterLSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2112
ER  - 

@conference{
author = "Kostić, Igor and Milenkovic, Ivan and Nikolić, Nina and Milanovic, Slobodan and Kostić Kravljanac, Ljiljana and Bosnić, Predrag and Paravinja, Ana",
year = "2022",
abstract = "Pedunculate oak (Quercus robur L.) is the most abundant deciduous tree species in Europe with high economical and ecological importance. Different species of Phytophthora are considered as one of the most important factors responsible for deterioration of oak forest, causing serious root damage not only in the forest trees, but also in the nurseries. Oak seedlings were grown in plastic pots with extremely low phosphorus (P) soil (1.5 mg kg-1 total P; no available Olsen-P detected). Silicon (Si) and P were supplied as Na2SiO3 (300 mg Si kg-1 dry soil) and KH2PO4 (180 mg P kg-1 dry soil), respectively. Four treatments (-P/-Si, -P+/Si, +P/-Si, and +P/+Si) were used in the experiment. After two months of experiment, a half of the plants in each treatment were root-inoculated with Phytophthora plurivora. After further four weeks, the first symptoms of P. plurivora infection appeared in leaves (e.g., leaf necrosis and wilting). Plants were then carefully removed from the pots, divided into roots and shoots, and the roots were scanned and analyzed by the WinRHIZO® software. Foliar concentrations of Si, P, K, Ca, Mg, B, Cu, Fe, Mn, and Zn were determined by ICP-OES, while the concentrations of N and S were determined by CHNS Analyzer. The addition of Si obviously improved root health status (e.g., decreasing de number of lesions and necrosis intensity) in the infected plants grown under -P conditions, which was followed by an increased foliar P concentration. The Si supply significantly increased the root variables (e.g., total root volume, root length, and area of thin roots) in both -P and +P plants inoculated with P. plurivora. Therefore, P. plurivora infection and supply of P and Si modulated the nutrient uptake and thereby changed the leaf ionomics, especially for infected -P plants supplied with Si (e.g., significantly increased B, Cu, and Si foliar concentrations and decreased Fe, Mn, Ca, Mg, K, and S foliar concentrations). Furthermore, Si fertilization significantly declined loses in plant dry biomass caused by P. plurivora infection and/or P deficiency, showing biomass comparable to non-infected +P plants.",
publisher = "ISSAG and AgCenterLSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2112"
}

Kostić, I., Milenkovic, I., Nikolić, N., Milanovic, S., Kostić Kravljanac, L., Bosnić, P.,& Paravinja, A.. (2022). Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenterLSU., 19.
https://hdl.handle.net/21.15107/rcub_rimsi_2112

Kostić I, Milenkovic I, Nikolić N, Milanovic S, Kostić Kravljanac L, Bosnić P, Paravinja A. Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;:19.
https://hdl.handle.net/21.15107/rcub_rimsi_2112 .

Kostić, Igor, Milenkovic, Ivan, Nikolić, Nina, Milanovic, Slobodan, Kostić Kravljanac, Ljiljana, Bosnić, Predrag, Paravinja, Ana, "Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022):19,
https://hdl.handle.net/21.15107/rcub_rimsi_2112 .

TY  - JOUR
AU  - Pavlović, Jelena
AU  - Kostić Kravljanac, Ljiljana
AU  - Bosnić, Predrag
AU  - Kirkby, Ernest A.
AU  - Nikolic, Miroslav
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1425
AB  - Silicon (Si) is not classified as an essential element for plants, but numerous studies have demonstrated its beneficial effects in a variety of species and environmental conditions, including low nutrient availability. Application of Si shows the potential to increase nutrient availability in the rhizosphere and root uptake through complex mechanisms, which still remain unclear. Silicon-mediated transcriptional regulation of element transporters for both root acquisition and tissue homeostasis has recently been suggested as an important strategy, varying in detail depending on plant species and nutritional status. Here, we summarize evidence of Si-mediated acquisition, uptake and translocation of nutrients: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), iron (Fe), zinc (Zn), manganese (Mn), copper (Cu), boron (B), chlorine (Cl), and nickel (Ni) under both deficiency and excess conditions. In addition, we discuss interactions of Si-with beneficial elements: aluminum (Al), sodium (Na), and selenium (Se). This review also highlights further research needed to improve understanding of Si-mediated acquisition and utilization of nutrients and vice versa nutrient status-mediated Si acquisition and transport, both processes which are of high importance for agronomic practice (e.g., reduced use of fertilizers and pesticides).
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Interactions of Silicon With Essential and Beneficial Elements in Plants
VL  - 12
DO  - 10.3389/fpls.2021.697592
ER  - 

@article{
author = "Pavlović, Jelena and Kostić Kravljanac, Ljiljana and Bosnić, Predrag and Kirkby, Ernest A. and Nikolic, Miroslav",
year = "2021",
abstract = "Silicon (Si) is not classified as an essential element for plants, but numerous studies have demonstrated its beneficial effects in a variety of species and environmental conditions, including low nutrient availability. Application of Si shows the potential to increase nutrient availability in the rhizosphere and root uptake through complex mechanisms, which still remain unclear. Silicon-mediated transcriptional regulation of element transporters for both root acquisition and tissue homeostasis has recently been suggested as an important strategy, varying in detail depending on plant species and nutritional status. Here, we summarize evidence of Si-mediated acquisition, uptake and translocation of nutrients: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), iron (Fe), zinc (Zn), manganese (Mn), copper (Cu), boron (B), chlorine (Cl), and nickel (Ni) under both deficiency and excess conditions. In addition, we discuss interactions of Si-with beneficial elements: aluminum (Al), sodium (Na), and selenium (Se). This review also highlights further research needed to improve understanding of Si-mediated acquisition and utilization of nutrients and vice versa nutrient status-mediated Si acquisition and transport, both processes which are of high importance for agronomic practice (e.g., reduced use of fertilizers and pesticides).",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Interactions of Silicon With Essential and Beneficial Elements in Plants",
volume = "12",
doi = "10.3389/fpls.2021.697592"
}

Pavlović, J., Kostić Kravljanac, L., Bosnić, P., Kirkby, E. A.,& Nikolic, M.. (2021). Interactions of Silicon With Essential and Beneficial Elements in Plants. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 12.
https://doi.org/10.3389/fpls.2021.697592

Pavlović J, Kostić Kravljanac L, Bosnić P, Kirkby EA, Nikolic M. Interactions of Silicon With Essential and Beneficial Elements in Plants. in Frontiers in Plant Science. 2021;12.
doi:10.3389/fpls.2021.697592 .

Pavlović, Jelena, Kostić Kravljanac, Ljiljana, Bosnić, Predrag, Kirkby, Ernest A., Nikolic, Miroslav, "Interactions of Silicon With Essential and Beneficial Elements in Plants" in Frontiers in Plant Science, 12 (2021),
https://doi.org/10.3389/fpls.2021.697592 . .

TY  - JOUR
AU  - Stefanović, Dejan
AU  - Nikolić, Nina
AU  - Kostić Kravljanac, Ljiljana
AU  - Todic, Slavica
AU  - Nikolic, Miroslav
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1446
AB  - Cluster zone leaf removal is a well-established viticulture practice for improving cluster microclimate and wine quality in cooler climates, while its efficacy in warmer conditions is less is known. Here we compared the effect of early (ELR, after fruit set; diameter of berries 3-5 mm) and late (LLR, beginning of veraison) leaf removal on berry composition and wine phenolic profile of grapevine (Vitis vinifera L.) variety Cabernet Sauvignon grown in a temperate, warmer region of Eastern Serbia. Compared to the control (no leaf removal), both leaf removal treatments increased the sugar content in fresh juice and alcohol concentration in wine. Over three consecutive years (2011-2013) markedly different in temperature and rainfall, ELR was clearly most effective in decreasing weights of cluster and of one berry, and in increasing of skin share in a berry. The content of total phenols, tartaric acid esters, anthocyanins, and flavanols in berry skin and wine was the highest in ELR treatment. ELR prominently modified the phenolic profile: Increasing flavanols, myricetin and quercetine in skin and wine, and anthocyanins, peonidin-3-glucoside in skin and delphinidin-3-glucoside in wine. This work demonstrated that early leaf removal positively influenced the chemical composition of berries and wine of Cabernet Sauvignon and might be recommended for practice in the temperate warm conditions.
PB  - MDPI, Basel
T2  - Agronomy-Basel
T1  - Early Leaf Removal Increases Berry and Wine Phenolics in Cabernet Sauvignon Grown in Eastern Serbia
IS  - 2
VL  - 11
DO  - 10.3390/agronomy11020238
ER  - 

@article{
author = "Stefanović, Dejan and Nikolić, Nina and Kostić Kravljanac, Ljiljana and Todic, Slavica and Nikolic, Miroslav",
year = "2021",
abstract = "Cluster zone leaf removal is a well-established viticulture practice for improving cluster microclimate and wine quality in cooler climates, while its efficacy in warmer conditions is less is known. Here we compared the effect of early (ELR, after fruit set; diameter of berries 3-5 mm) and late (LLR, beginning of veraison) leaf removal on berry composition and wine phenolic profile of grapevine (Vitis vinifera L.) variety Cabernet Sauvignon grown in a temperate, warmer region of Eastern Serbia. Compared to the control (no leaf removal), both leaf removal treatments increased the sugar content in fresh juice and alcohol concentration in wine. Over three consecutive years (2011-2013) markedly different in temperature and rainfall, ELR was clearly most effective in decreasing weights of cluster and of one berry, and in increasing of skin share in a berry. The content of total phenols, tartaric acid esters, anthocyanins, and flavanols in berry skin and wine was the highest in ELR treatment. ELR prominently modified the phenolic profile: Increasing flavanols, myricetin and quercetine in skin and wine, and anthocyanins, peonidin-3-glucoside in skin and delphinidin-3-glucoside in wine. This work demonstrated that early leaf removal positively influenced the chemical composition of berries and wine of Cabernet Sauvignon and might be recommended for practice in the temperate warm conditions.",
publisher = "MDPI, Basel",
journal = "Agronomy-Basel",
title = "Early Leaf Removal Increases Berry and Wine Phenolics in Cabernet Sauvignon Grown in Eastern Serbia",
number = "2",
volume = "11",
doi = "10.3390/agronomy11020238"
}

Stefanović, D., Nikolić, N., Kostić Kravljanac, L., Todic, S.,& Nikolic, M.. (2021). Early Leaf Removal Increases Berry and Wine Phenolics in Cabernet Sauvignon Grown in Eastern Serbia. in Agronomy-Basel
MDPI, Basel., 11(2).
https://doi.org/10.3390/agronomy11020238

Stefanović D, Nikolić N, Kostić Kravljanac L, Todic S, Nikolic M. Early Leaf Removal Increases Berry and Wine Phenolics in Cabernet Sauvignon Grown in Eastern Serbia. in Agronomy-Basel. 2021;11(2).
doi:10.3390/agronomy11020238 .

Stefanović, Dejan, Nikolić, Nina, Kostić Kravljanac, Ljiljana, Todic, Slavica, Nikolic, Miroslav, "Early Leaf Removal Increases Berry and Wine Phenolics in Cabernet Sauvignon Grown in Eastern Serbia" in Agronomy-Basel, 11, no. 2 (2021),
https://doi.org/10.3390/agronomy11020238 . .

TY  - JOUR
AU  - Tamindzic, Gordana
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Nikolic, Zorica
AU  - Kostić Kravljanac, Ljiljana
AU  - Jovicic, Dusica
AU  - Dolijanović, Zeljko
AU  - Savić, Jasna
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1441
AB  - Delivery of micronutrients to plants through seed priming improves seedling vigour and increases crops yields. Two-year filed trial was conducted in Pancevo, Serbia, with aim to study the effect of seed priming with zinc (Zn) on field performance of three maize hybrids on calcareous chernozem deficient in plant available Zn. Seed printing treatments were: control (without priming), water priming and priming with 4 mM zinc sulphate water solution. Seed priming had significant effect on early plant growth, plant height, yield components, grain yield and grain Zn concentration. Zn-priming promoted plant growth and increased final plant height. Across two growing seasons with contrasting precipitation and three tested maize hybrids, Zn-priming resulted in an average increase of grain yield by about 18% compared to control, and by about 8.4% compared to water priming. A significant relationship between plant growth parameters, grain yield components and grain yield was detected. Grain Zn concentration was increased by Zn-priming in two hybrids in the season with less precipitation and in one hybrid in the second season. The results imply that using the seeds with elevated Zn content can improve overall field performance of maize grown on calcareous chernozem.
PB  - Pagepress Publ, Pavia
T2  - Italian Journal of Agronomy
T1  - Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem
IS  - 3
VL  - 16
DO  - 10.4081/ija.2021.1795
ER  - 

@article{
author = "Tamindzic, Gordana and Ignjatov, Maja and Milošević, Dragana and Nikolic, Zorica and Kostić Kravljanac, Ljiljana and Jovicic, Dusica and Dolijanović, Zeljko and Savić, Jasna",
year = "2021",
abstract = "Delivery of micronutrients to plants through seed priming improves seedling vigour and increases crops yields. Two-year filed trial was conducted in Pancevo, Serbia, with aim to study the effect of seed priming with zinc (Zn) on field performance of three maize hybrids on calcareous chernozem deficient in plant available Zn. Seed printing treatments were: control (without priming), water priming and priming with 4 mM zinc sulphate water solution. Seed priming had significant effect on early plant growth, plant height, yield components, grain yield and grain Zn concentration. Zn-priming promoted plant growth and increased final plant height. Across two growing seasons with contrasting precipitation and three tested maize hybrids, Zn-priming resulted in an average increase of grain yield by about 18% compared to control, and by about 8.4% compared to water priming. A significant relationship between plant growth parameters, grain yield components and grain yield was detected. Grain Zn concentration was increased by Zn-priming in two hybrids in the season with less precipitation and in one hybrid in the second season. The results imply that using the seeds with elevated Zn content can improve overall field performance of maize grown on calcareous chernozem.",
publisher = "Pagepress Publ, Pavia",
journal = "Italian Journal of Agronomy",
title = "Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem",
number = "3",
volume = "16",
doi = "10.4081/ija.2021.1795"
}

Tamindzic, G., Ignjatov, M., Milošević, D., Nikolic, Z., Kostić Kravljanac, L., Jovicic, D., Dolijanović, Z.,& Savić, J.. (2021). Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem. in Italian Journal of Agronomy
Pagepress Publ, Pavia., 16(3).
https://doi.org/10.4081/ija.2021.1795

Tamindzic G, Ignjatov M, Milošević D, Nikolic Z, Kostić Kravljanac L, Jovicic D, Dolijanović Z, Savić J. Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem. in Italian Journal of Agronomy. 2021;16(3).
doi:10.4081/ija.2021.1795 .

Tamindzic, Gordana, Ignjatov, Maja, Milošević, Dragana, Nikolic, Zorica, Kostić Kravljanac, Ljiljana, Jovicic, Dusica, Dolijanović, Zeljko, Savić, Jasna, "Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem" in Italian Journal of Agronomy, 16, no. 3 (2021),
https://doi.org/10.4081/ija.2021.1795 . .

TY  - CONF
AU  - Milenković, Ivana
AU  - Bartolić, Dragana
AU  - Algarra, Manuel
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolic, Miroslav
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1848
AB  - Carbon dots (CDs) are popular and emerging nanomaterial which found application in many fields such as drug delivery, optoelectronic, and imaging due to their high solubility, low cost and easiness of their functionalization. Their effect on plants is not sufficiently investigated, so it is necessary to investigate their ecotoxicity. In this research, CDs prepared from folic acid was used for the treatment of maize plants with two different concentrations. The treatment was performed during the plant growth in hydroponics. ICP method was used for the analysis of macronutrients (Ca, K, Mg, P, S) uptake in plants from the hydroponic medium, which was used for the plant growth. The obtained TPC results demonstrated low oxidative stress proportional to the used concentration, which was not significant. The similar trend was observed in TAA where the only significant increase was in plant shoots after the treatment at 500 µg L-1.
PB  - University of Belgrade
PB  - Technical Faculty in Bor
C3  - 27th International Conference Ecological Truth and Environmental Research - EcoTER '19
T1  - The examination of ecotoxic effect of folic acid based carbon dots on maize
EP  - 310
SP  - 305
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1848
ER  - 

@conference{
author = "Milenković, Ivana and Bartolić, Dragana and Algarra, Manuel and Kostić Kravljanac, Ljiljana and Nikolic, Miroslav and Radotić, Ksenija",
year = "2019",
abstract = "Carbon dots (CDs) are popular and emerging nanomaterial which found application in many fields such as drug delivery, optoelectronic, and imaging due to their high solubility, low cost and easiness of their functionalization. Their effect on plants is not sufficiently investigated, so it is necessary to investigate their ecotoxicity. In this research, CDs prepared from folic acid was used for the treatment of maize plants with two different concentrations. The treatment was performed during the plant growth in hydroponics. ICP method was used for the analysis of macronutrients (Ca, K, Mg, P, S) uptake in plants from the hydroponic medium, which was used for the plant growth. The obtained TPC results demonstrated low oxidative stress proportional to the used concentration, which was not significant. The similar trend was observed in TAA where the only significant increase was in plant shoots after the treatment at 500 µg L-1.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "27th International Conference Ecological Truth and Environmental Research - EcoTER '19",
title = "The examination of ecotoxic effect of folic acid based carbon dots on maize",
pages = "310-305",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1848"
}

Milenković, I., Bartolić, D., Algarra, M., Kostić Kravljanac, L., Nikolic, M.,& Radotić, K.. (2019). The examination of ecotoxic effect of folic acid based carbon dots on maize. in 27th International Conference Ecological Truth and Environmental Research - EcoTER '19
University of Belgrade., 305-310.
https://hdl.handle.net/21.15107/rcub_rimsi_1848

Milenković I, Bartolić D, Algarra M, Kostić Kravljanac L, Nikolic M, Radotić K. The examination of ecotoxic effect of folic acid based carbon dots on maize. in 27th International Conference Ecological Truth and Environmental Research - EcoTER '19. 2019;:305-310.
https://hdl.handle.net/21.15107/rcub_rimsi_1848 .

Milenković, Ivana, Bartolić, Dragana, Algarra, Manuel, Kostić Kravljanac, Ljiljana, Nikolic, Miroslav, Radotić, Ksenija, "The examination of ecotoxic effect of folic acid based carbon dots on maize" in 27th International Conference Ecological Truth and Environmental Research - EcoTER '19 (2019):305-310,
https://hdl.handle.net/21.15107/rcub_rimsi_1848 .

TY  - JOUR
AU  - Nikolic, Dragana B.
AU  - Nesic, Sofija
AU  - Bosnic, Dragana
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolic, Miroslav
AU  - Samardžić, Jelena T.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1284
AB  - The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
VL  - 10
DO  - 10.3389/fpls.2019.00416
ER  - 

@article{
author = "Nikolic, Dragana B. and Nesic, Sofija and Bosnic, Dragana and Kostić Kravljanac, Ljiljana and Nikolic, Miroslav and Samardžić, Jelena T.",
year = "2019",
abstract = "The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution",
volume = "10",
doi = "10.3389/fpls.2019.00416"
}

Nikolic, D. B., Nesic, S., Bosnic, D., Kostić Kravljanac, L., Nikolic, M.,& Samardžić, J. T.. (2019). Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fpls.2019.00416

Nikolic DB, Nesic S, Bosnic D, Kostić Kravljanac L, Nikolic M, Samardžić JT. Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science. 2019;10.
doi:10.3389/fpls.2019.00416 .

Nikolic, Dragana B., Nesic, Sofija, Bosnic, Dragana, Kostić Kravljanac, Ljiljana, Nikolic, Miroslav, Samardžić, Jelena T., "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution" in Frontiers in Plant Science, 10 (2019),
https://doi.org/10.3389/fpls.2019.00416 . .

TY  - CONF
AU  - Djordjevic, Pavle
AU  - Kostić, Ljiljana
AU  - Bosnić, Predrag
AU  - Maksimović, Vuk
AU  - TODIC, Slavica
AU  - Nikolic, Miroslav
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2511
AB  - Low phosphorus (P) availability is a common constraint for crop productivity in both acid and
calcareous soils due to formation of insoluble P complexes. As far as we are aware, grapevine is not
considered a P-efficient species, whereas P deficiency is rarely reported under the field conditions.
We performed a field experiment under extremely low P conditions (Olsen P < 2 mg kg-1) using
own-designed rhizotron (80 cm depth), installed in 8-y-old vineyard with cultivar Chardonnay,
grafted on 5BB rootstock. The device enables easy access to the new intact roots while preventing
root drying. We collected the exudates from root tips every 2 h (during daytime) at the different
growth stages (i.e. shoots 10 cm, flowering, berries pea-size and veraison). At the same time, both
rhizosphere and bulk soil samples were collected and subjected to the sequential P analyses. The
results of HPLC analyses of the root exudates showed that citrate can be considered as the main
P-mobilizing compound, with clear seasonal and diurnal patterns: the highest anion exudation
rate was recorded at flowering (lower pick) and veraison (higher pick), and at each growth phase
in the morning (10 a.m.) and in the afternoon (4 p.m.), with a rapid depression at 1-3 p.m. Consequently,
the relative share of soluble P fractions (H2O- and NaHCO3-extractable) in the rhizosphere
was very high (20% of the total P fractions), leading to the leaf P concentration of 0.2% DW which
is above the critical P-deficient level (0.15% DW) for grapevine leaves at flowering.
PB  - Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade
C3  - Book of Abstracts: 3rd International Conference on Plant Biology [and] 22nd SPPS Meeting, 9-12 June 2018, Belgrade
T1  - Seasonal dynamics of the rhizosphere phosphorus and citrate exudation by grapevine roots in a low P soil: a field experiment
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2511
ER  - 

@conference{
author = "Djordjevic, Pavle and Kostić, Ljiljana and Bosnić, Predrag and Maksimović, Vuk and TODIC, Slavica and Nikolic, Miroslav",
year = "2018",
abstract = "Low phosphorus (P) availability is a common constraint for crop productivity in both acid and
calcareous soils due to formation of insoluble P complexes. As far as we are aware, grapevine is not
considered a P-efficient species, whereas P deficiency is rarely reported under the field conditions.
We performed a field experiment under extremely low P conditions (Olsen P < 2 mg kg-1) using
own-designed rhizotron (80 cm depth), installed in 8-y-old vineyard with cultivar Chardonnay,
grafted on 5BB rootstock. The device enables easy access to the new intact roots while preventing
root drying. We collected the exudates from root tips every 2 h (during daytime) at the different
growth stages (i.e. shoots 10 cm, flowering, berries pea-size and veraison). At the same time, both
rhizosphere and bulk soil samples were collected and subjected to the sequential P analyses. The
results of HPLC analyses of the root exudates showed that citrate can be considered as the main
P-mobilizing compound, with clear seasonal and diurnal patterns: the highest anion exudation
rate was recorded at flowering (lower pick) and veraison (higher pick), and at each growth phase
in the morning (10 a.m.) and in the afternoon (4 p.m.), with a rapid depression at 1-3 p.m. Consequently,
the relative share of soluble P fractions (H2O- and NaHCO3-extractable) in the rhizosphere
was very high (20% of the total P fractions), leading to the leaf P concentration of 0.2% DW which
is above the critical P-deficient level (0.15% DW) for grapevine leaves at flowering.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade",
journal = "Book of Abstracts: 3rd International Conference on Plant Biology [and] 22nd SPPS Meeting, 9-12 June 2018, Belgrade",
title = "Seasonal dynamics of the rhizosphere phosphorus and citrate exudation by grapevine roots in a low P soil: a field experiment",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2511"
}

Djordjevic, P., Kostić, L., Bosnić, P., Maksimović, V., TODIC, S.,& Nikolic, M.. (2018). Seasonal dynamics of the rhizosphere phosphorus and citrate exudation by grapevine roots in a low P soil: a field experiment. in Book of Abstracts: 3rd International Conference on Plant Biology [and] 22nd SPPS Meeting, 9-12 June 2018, Belgrade
Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2511

Djordjevic P, Kostić L, Bosnić P, Maksimović V, TODIC S, Nikolic M. Seasonal dynamics of the rhizosphere phosphorus and citrate exudation by grapevine roots in a low P soil: a field experiment. in Book of Abstracts: 3rd International Conference on Plant Biology [and] 22nd SPPS Meeting, 9-12 June 2018, Belgrade. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2511 .

Djordjevic, Pavle, Kostić, Ljiljana, Bosnić, Predrag, Maksimović, Vuk, TODIC, Slavica, Nikolic, Miroslav, "Seasonal dynamics of the rhizosphere phosphorus and citrate exudation by grapevine roots in a low P soil: a field experiment" in Book of Abstracts: 3rd International Conference on Plant Biology [and] 22nd SPPS Meeting, 9-12 June 2018, Belgrade (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2511 .

TY  - CONF
AU  - Nikolic, Dragana B.
AU  - Nesic, Sofija
AU  - Bosnic, Dragana
AU  - Kostić, Ljiljana
AU  - Nikolic, Miroslav
AU  - Samardzic, Jelena
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2478
AB  - The beneficial effects of silicon (Si) on various abiotic and biotic stresses in plants are well established; however, molecular
mechanisms are not completely understood. An ameliorative effect of Si on iron (Fe) deficiency stress has only been shown
on plants which use the reduction-based strategy (Strategy I) for Fe acquisition. The aim of our study was to investigate
influence of Si on Fe deficiency stress alleviation in a cereal plant which uses the chelation-based strategy (Strategy II) for
Fe acquisition, and barley was chosen as a representative.
Si successfully ameliorated Fe deficiency in barley, attenuating chlorosis and biomass loss of the youngest leaves, as well
as ROS accumulation, accompanied with the recovered activities of antioxidative enzymes, ascorbate peroxidase and
catalase. Si increased Fe content in the youngest leaves of Fe deprived plants, as well as Fe concentration in the watersoluble
(w-s) fraction. On the other hand, w-s concentration and total content of optimally supplied microelements, Mn and
Zn, were decreased in Si supplied plants. The expression of Strategy II genes was modulated under the influence of Si. An
expeditious increase in the gene expression was detected in Fe deficient roots. Moreover, a dramatic Si-promoted
upregulation of some of the investigated genes was detected in leaves.
Fe deficiency in plants due to low Fe availability in soils has a considerable impact on both yield and nutritional value of
crops. New findings presented in our study may support development of strategies to overcome this substantial agricultural
problem.
PB  - University of Copenhagen, Denmark
C3  - Plant Biology Europe 2018 Conference Abstract Book
T1  - The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2478
ER  - 

@conference{
author = "Nikolic, Dragana B. and Nesic, Sofija and Bosnic, Dragana and Kostić, Ljiljana and Nikolic, Miroslav and Samardzic, Jelena",
year = "2018",
abstract = "The beneficial effects of silicon (Si) on various abiotic and biotic stresses in plants are well established; however, molecular
mechanisms are not completely understood. An ameliorative effect of Si on iron (Fe) deficiency stress has only been shown
on plants which use the reduction-based strategy (Strategy I) for Fe acquisition. The aim of our study was to investigate
influence of Si on Fe deficiency stress alleviation in a cereal plant which uses the chelation-based strategy (Strategy II) for
Fe acquisition, and barley was chosen as a representative.
Si successfully ameliorated Fe deficiency in barley, attenuating chlorosis and biomass loss of the youngest leaves, as well
as ROS accumulation, accompanied with the recovered activities of antioxidative enzymes, ascorbate peroxidase and
catalase. Si increased Fe content in the youngest leaves of Fe deprived plants, as well as Fe concentration in the watersoluble
(w-s) fraction. On the other hand, w-s concentration and total content of optimally supplied microelements, Mn and
Zn, were decreased in Si supplied plants. The expression of Strategy II genes was modulated under the influence of Si. An
expeditious increase in the gene expression was detected in Fe deficient roots. Moreover, a dramatic Si-promoted
upregulation of some of the investigated genes was detected in leaves.
Fe deficiency in plants due to low Fe availability in soils has a considerable impact on both yield and nutritional value of
crops. New findings presented in our study may support development of strategies to overcome this substantial agricultural
problem.",
publisher = "University of Copenhagen, Denmark",
journal = "Plant Biology Europe 2018 Conference Abstract Book",
title = "The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2478"
}

Nikolic, D. B., Nesic, S., Bosnic, D., Kostić, L., Nikolic, M.,& Samardzic, J.. (2018). The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution. in Plant Biology Europe 2018 Conference Abstract Book
University of Copenhagen, Denmark..
https://hdl.handle.net/21.15107/rcub_rimsi_2478

Nikolic DB, Nesic S, Bosnic D, Kostić L, Nikolic M, Samardzic J. The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution. in Plant Biology Europe 2018 Conference Abstract Book. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2478 .

Nikolic, Dragana B., Nesic, Sofija, Bosnic, Dragana, Kostić, Ljiljana, Nikolic, Miroslav, Samardzic, Jelena, "The beneficial effects of Si on iron deficiency stress alleviation in barley: modulation of Strategy II genes expression and metal redistribution" in Plant Biology Europe 2018 Conference Abstract Book (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2478 .

TY  - JOUR
AU  - Nikolić, Nina
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolic, Miroslav
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1172
AB  - Long-term potentials and constraints of nature to spontaneously recover after severe degradation by toxic mine waste, and general principles of mutual modifications of spontaneous vegetation and soils during this process, have remained widely unknown. This study investigates the effect of flooding on natural restoration of a model locality in Eastern Serbia, complexly degraded by 70-years fluvial deposition of sulfidic copper (Cu) mine tailings in a floodplain along 80km of the polluted river flow. We analyzed multivariate response of forest vegetation (floristic and structural parameters) together with physical and chemical properties of concomitant soils and sediments. Floods strongly modify the interactions between soil and vegetation. Three very different types of forest vegetation constitute the response of the nature to key soil adverse factors (excessive Cu availability, low nutrients, and low pH); combined with drought, these constraints completely inhibit revegetation. Continual flooding after mine closure, despite the pollution it still brings in, fosters a faster development of highly specialized vegetation and most importantly faster buildup of soil organic matter necessary for phytostabilization of substantial amounts of Cu tailings present in the floodplain. We show that enhanced nutrient fluxes facilitated by natural flooding regime might overrun the constraining effect of deposited Cu also for natural vegetation.
PB  - Wiley, Hoboken
T2  - Land Degradation & Development
T1  - To dam, or not to dam? Abolishment of further flooding impedes the natural revegetation processes after long-term fluvial deposition of copper tailings
EP  - 1924
IS  - 6
SP  - 1915
VL  - 29
DO  - 10.1002/ldr.2921
ER  - 

@article{
author = "Nikolić, Nina and Kostić Kravljanac, Ljiljana and Nikolic, Miroslav",
year = "2018",
abstract = "Long-term potentials and constraints of nature to spontaneously recover after severe degradation by toxic mine waste, and general principles of mutual modifications of spontaneous vegetation and soils during this process, have remained widely unknown. This study investigates the effect of flooding on natural restoration of a model locality in Eastern Serbia, complexly degraded by 70-years fluvial deposition of sulfidic copper (Cu) mine tailings in a floodplain along 80km of the polluted river flow. We analyzed multivariate response of forest vegetation (floristic and structural parameters) together with physical and chemical properties of concomitant soils and sediments. Floods strongly modify the interactions between soil and vegetation. Three very different types of forest vegetation constitute the response of the nature to key soil adverse factors (excessive Cu availability, low nutrients, and low pH); combined with drought, these constraints completely inhibit revegetation. Continual flooding after mine closure, despite the pollution it still brings in, fosters a faster development of highly specialized vegetation and most importantly faster buildup of soil organic matter necessary for phytostabilization of substantial amounts of Cu tailings present in the floodplain. We show that enhanced nutrient fluxes facilitated by natural flooding regime might overrun the constraining effect of deposited Cu also for natural vegetation.",
publisher = "Wiley, Hoboken",
journal = "Land Degradation & Development",
title = "To dam, or not to dam? Abolishment of further flooding impedes the natural revegetation processes after long-term fluvial deposition of copper tailings",
pages = "1924-1915",
number = "6",
volume = "29",
doi = "10.1002/ldr.2921"
}

Nikolić, N., Kostić Kravljanac, L.,& Nikolic, M.. (2018). To dam, or not to dam? Abolishment of further flooding impedes the natural revegetation processes after long-term fluvial deposition of copper tailings. in Land Degradation & Development
Wiley, Hoboken., 29(6), 1915-1924.
https://doi.org/10.1002/ldr.2921

Nikolić N, Kostić Kravljanac L, Nikolic M. To dam, or not to dam? Abolishment of further flooding impedes the natural revegetation processes after long-term fluvial deposition of copper tailings. in Land Degradation & Development. 2018;29(6):1915-1924.
doi:10.1002/ldr.2921 .

Nikolić, Nina, Kostić Kravljanac, Ljiljana, Nikolic, Miroslav, "To dam, or not to dam? Abolishment of further flooding impedes the natural revegetation processes after long-term fluvial deposition of copper tailings" in Land Degradation & Development, 29, no. 6 (2018):1915-1924,
https://doi.org/10.1002/ldr.2921 . .

TY  - CONF
AU  - Nikolic, Miroslav
AU  - Kostić Kravljanac, Ljiljana
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2149
AB  - Silicon is the only known mineral element that effectively alleviates multiple environmental stress in many plant species. Over the past decade rapid progress has been made in understanding the mechanisms through which Si mediates mineral excess and/or toxicity stress. It has been demonstrated that Si mediates uptake and transport of mineral elements at excess by regulating expression of various transporter genes (e.g. Kim et al., 2014; Akcay and Erkan, 2016; Che et al., 2016); however, the role of Si in nutrient uptake and transport under nutrient deficiency conditions is still insufficiently understood. In this presentation, I will talk about Si influence on (a) root P- starvation responses for rhizosphere mobilization and uptake of Pi in wheat (Triticum aestivum) and (b) acquisition and long-distance transport of Fe in cucumber (Cucumis sativus) under low Fe conditions; our recent unpublished work on (c) Si-regulated expression of the transporters involved in Na homeostasis in maize (Zea mays) subjected to NaCl stress will also be discussed.
PB  - University of Copenhagen, Denmark
C3  - XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark
T1  - Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2149
ER  - 

@conference{
author = "Nikolic, Miroslav and Kostić Kravljanac, Ljiljana and Pavlović, Jelena and Bosnić, Predrag",
year = "2017",
abstract = "Silicon is the only known mineral element that effectively alleviates multiple environmental stress in many plant species. Over the past decade rapid progress has been made in understanding the mechanisms through which Si mediates mineral excess and/or toxicity stress. It has been demonstrated that Si mediates uptake and transport of mineral elements at excess by regulating expression of various transporter genes (e.g. Kim et al., 2014; Akcay and Erkan, 2016; Che et al., 2016); however, the role of Si in nutrient uptake and transport under nutrient deficiency conditions is still insufficiently understood. In this presentation, I will talk about Si influence on (a) root P- starvation responses for rhizosphere mobilization and uptake of Pi in wheat (Triticum aestivum) and (b) acquisition and long-distance transport of Fe in cucumber (Cucumis sativus) under low Fe conditions; our recent unpublished work on (c) Si-regulated expression of the transporters involved in Na homeostasis in maize (Zea mays) subjected to NaCl stress will also be discussed.",
publisher = "University of Copenhagen, Denmark",
journal = "XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark",
title = "Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2149"
}

Nikolic, M., Kostić Kravljanac, L., Pavlović, J.,& Bosnić, P.. (2017). Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress. in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark
University of Copenhagen, Denmark..
https://hdl.handle.net/21.15107/rcub_rimsi_2149

Nikolic M, Kostić Kravljanac L, Pavlović J, Bosnić P. Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress. in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark. 2017;.
https://hdl.handle.net/21.15107/rcub_rimsi_2149 .

Nikolic, Miroslav, Kostić Kravljanac, Ljiljana, Pavlović, Jelena, Bosnić, Predrag, "Silicon mediates ion uptake, transport and homeostasis in plants under mineral stress" in XVIII International Plant Nutrition Colloquium with Boron and Manganese Satellite Meetings, August 19-24, 2017, Copenhagen, Denmark (2017),
https://hdl.handle.net/21.15107/rcub_rimsi_2149 .

TY  - CONF
AU  - Nikolic, Miroslav
AU  - Kostić Kravljanac, Ljiljana
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2141
AB  - The plant ionomics is the study of essential and nonessential mineral element composition of plants
(the ionom) at cellular, tissue or organismal level. The plant ionomic profile is affected by various
factors, including plant (e.g. species, genotypes, organ, developmental change) and environment
(e.g. soil, fertilizers, stress conditions). Over the past decade rapid progress has been made in
understanding the mechanisms through which silicon (Si) mediates mineral excess and/or toxicity
stress. However, the effect of Si on the mineral element uptake and consequently the plant ionome
is still unclear, in particular under conditions of limited nutrient availability.
Firstly, I will present recent results of my research group demonstrating that Si application modulates
the ionomic profile of various plant species (e.g. rice, barley, wheat, maize, cucumber, sunflower,
soybean, grapevine and tomato) grown under both normal and stress conditions. In the second
part of my talk I will review the current knowledge of Si influence on the expression of (a) root
and shoot metal transporter genes under excess of cadmium (Cd), manganese (Mn) and copper
(Cu) (Li et al., 2018; Kim et al. 2014; Che et al., 2016; Farooq et al., 2016); (b) transporter genes
involved in the uptake, long-distance transport and homeostasis of iron (Fe) under low Fe
conditions (Pavlovic et al., 2013, 2016); (c) transporter genes for inorganic phosphorus (Pi) root uptake
under low P conditions (Kostic et al., manuscript submitted); and (d) transporter genes involved in
shoot homeostasis of sodium (Na+) (see Bosnic et al., this proceedings) and B (Akcay & Erkan, 2016)
under saline stress.
In conclusion, the role of Si in modulation of plant ionome, including also nutrient and other mineral
element uptake and utilization, appears to be more indirect by transcriptional regulation of genes
responsible for both root acquisition and tissue homeostasis. Further understanding of how exactly
Si regulates the expression of mineral element transporter genes will help to improve crop
productivity, yield quality and food safety in stress conditions.
PB  - University of Agricultural Sciences, GKVK, Bengaluru
C3  - 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India
T1  - Silicon influence on plant ionome and mineral element transporters
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2141
ER  - 

@conference{
author = "Nikolic, Miroslav and Kostić Kravljanac, Ljiljana and Pavlović, Jelena and Bosnić, Predrag",
year = "2017",
abstract = "The plant ionomics is the study of essential and nonessential mineral element composition of plants
(the ionom) at cellular, tissue or organismal level. The plant ionomic profile is affected by various
factors, including plant (e.g. species, genotypes, organ, developmental change) and environment
(e.g. soil, fertilizers, stress conditions). Over the past decade rapid progress has been made in
understanding the mechanisms through which silicon (Si) mediates mineral excess and/or toxicity
stress. However, the effect of Si on the mineral element uptake and consequently the plant ionome
is still unclear, in particular under conditions of limited nutrient availability.
Firstly, I will present recent results of my research group demonstrating that Si application modulates
the ionomic profile of various plant species (e.g. rice, barley, wheat, maize, cucumber, sunflower,
soybean, grapevine and tomato) grown under both normal and stress conditions. In the second
part of my talk I will review the current knowledge of Si influence on the expression of (a) root
and shoot metal transporter genes under excess of cadmium (Cd), manganese (Mn) and copper
(Cu) (Li et al., 2018; Kim et al. 2014; Che et al., 2016; Farooq et al., 2016); (b) transporter genes
involved in the uptake, long-distance transport and homeostasis of iron (Fe) under low Fe
conditions (Pavlovic et al., 2013, 2016); (c) transporter genes for inorganic phosphorus (Pi) root uptake
under low P conditions (Kostic et al., manuscript submitted); and (d) transporter genes involved in
shoot homeostasis of sodium (Na+) (see Bosnic et al., this proceedings) and B (Akcay & Erkan, 2016)
under saline stress.
In conclusion, the role of Si in modulation of plant ionome, including also nutrient and other mineral
element uptake and utilization, appears to be more indirect by transcriptional regulation of genes
responsible for both root acquisition and tissue homeostasis. Further understanding of how exactly
Si regulates the expression of mineral element transporter genes will help to improve crop
productivity, yield quality and food safety in stress conditions.",
publisher = "University of Agricultural Sciences, GKVK, Bengaluru",
journal = "7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India",
title = "Silicon influence on plant ionome and mineral element transporters",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2141"
}

Nikolic, M., Kostić Kravljanac, L., Pavlović, J.,& Bosnić, P.. (2017). Silicon influence on plant ionome and mineral element transporters. in 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India
University of Agricultural Sciences, GKVK, Bengaluru..
https://hdl.handle.net/21.15107/rcub_rimsi_2141

Nikolic M, Kostić Kravljanac L, Pavlović J, Bosnić P. Silicon influence on plant ionome and mineral element transporters. in 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India. 2017;.
https://hdl.handle.net/21.15107/rcub_rimsi_2141 .

Nikolic, Miroslav, Kostić Kravljanac, Ljiljana, Pavlović, Jelena, Bosnić, Predrag, "Silicon influence on plant ionome and mineral element transporters" in 7th International Conference on Silicon in Agriculture, October 24-28, 2017, Bangaluru, India (2017),
https://hdl.handle.net/21.15107/rcub_rimsi_2141 .

TY  - JOUR
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolić, Nina
AU  - Bosnic, Dragana
AU  - Samardžić, Jelena T.
AU  - Nikolic, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1067
AB  - Although silicon (Si) is known to improve plant growth under low phosphorus (P) conditions, the in planta mechanisms responsible for this effect are still unknown. Here, we investigated the role of Si on P uptake along with the expression of Pi transporters in wheat (Triticum aestivum L.) grown in low P acid soil in comparison with P fertilization and liming. A combined approach was performed including analyses of rhizosphere soil, tissue P content, the expression of the root Pi transporter genes (TaPHT1.1 and TaPHT1.2), and the root exudation of citrate and malate. Supply of Si in a form of Na2SiO3 increased shoot P concentration to an adequate level in the range of P-fertilized plants. Silicon ameliorated low soil pH and high Al3+ comparable to the effect of liming. The in planta effect of Si on up-regulating the expression of TaPHT1.1 and TaPHT1.2 was several fold higher and consequently P uptake doubled compared to both P fertilization and liming. In addition, Si directly stimulated root Pi acquisition by prominently increasing both malate and citrate exudation rate. Application of Si increased root exudation of organic acids that mobilize Pi in the rhizosphere and up-regulated Pi transporters in wheat roots.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions
EP  - 455
IS  - 1-2
SP  - 447
VL  - 419
DO  - 10.1007/s11104-017-3364-0
ER  - 

@article{
author = "Kostić Kravljanac, Ljiljana and Nikolić, Nina and Bosnic, Dragana and Samardžić, Jelena T. and Nikolic, Miroslav",
year = "2017",
abstract = "Although silicon (Si) is known to improve plant growth under low phosphorus (P) conditions, the in planta mechanisms responsible for this effect are still unknown. Here, we investigated the role of Si on P uptake along with the expression of Pi transporters in wheat (Triticum aestivum L.) grown in low P acid soil in comparison with P fertilization and liming. A combined approach was performed including analyses of rhizosphere soil, tissue P content, the expression of the root Pi transporter genes (TaPHT1.1 and TaPHT1.2), and the root exudation of citrate and malate. Supply of Si in a form of Na2SiO3 increased shoot P concentration to an adequate level in the range of P-fertilized plants. Silicon ameliorated low soil pH and high Al3+ comparable to the effect of liming. The in planta effect of Si on up-regulating the expression of TaPHT1.1 and TaPHT1.2 was several fold higher and consequently P uptake doubled compared to both P fertilization and liming. In addition, Si directly stimulated root Pi acquisition by prominently increasing both malate and citrate exudation rate. Application of Si increased root exudation of organic acids that mobilize Pi in the rhizosphere and up-regulated Pi transporters in wheat roots.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions",
pages = "455-447",
number = "1-2",
volume = "419",
doi = "10.1007/s11104-017-3364-0"
}

Kostić Kravljanac, L., Nikolić, N., Bosnic, D., Samardžić, J. T.,& Nikolic, M.. (2017). Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions. in Plant and Soil
Springer, Dordrecht., 419(1-2), 447-455.
https://doi.org/10.1007/s11104-017-3364-0

Kostić Kravljanac L, Nikolić N, Bosnic D, Samardžić JT, Nikolic M. Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions. in Plant and Soil. 2017;419(1-2):447-455.
doi:10.1007/s11104-017-3364-0 .

Kostić Kravljanac, Ljiljana, Nikolić, Nina, Bosnic, Dragana, Samardžić, Jelena T., Nikolic, Miroslav, "Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions" in Plant and Soil, 419, no. 1-2 (2017):447-455,
https://doi.org/10.1007/s11104-017-3364-0 . .

TY  - JOUR
AU  - Nikolic, Miroslav
AU  - Nikolić, Nina
AU  - Kostić Kravljanac, Ljiljana
AU  - Pavlović, Jelena
AU  - Bosnić, Predrag
AU  - Stević, Nenad
AU  - Savić, Jasna
AU  - Hristov, Nikola
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/949
AB  - The deficiency of zinc (Zn) and iron (Fe) is a global issue causing not only considerable yield losses of food crops but also serious health problems. We have analysed Zn and Fe concentrations in the grains of two bread wheat cultivars along native gradient of micronutrient availability throughout Serbia. Although only 13% of the soil samples were Zn deficient and none was Fe deficient, the levels of these micronutrients in grain were rather low (median values of 21 mg kg(-1) for Zn and 36 mg kg(-1) for Fe), and even less adequate in white flour. Moreover, excessive P fertilization of calcareous soils in the major wheat growing areas strongly correlated with lower grain concentration of Zn. Our results imply that a latent Zn deficiency in wheat grain poses a high risk for grain quality relevant to human health in Serbia, where wheat bread is a staple food. k.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition
EP  - 148
SP  - 141
VL  - 553
DO  - 10.1016/j.scitotenv.2016.02.102
ER  - 

@article{
author = "Nikolic, Miroslav and Nikolić, Nina and Kostić Kravljanac, Ljiljana and Pavlović, Jelena and Bosnić, Predrag and Stević, Nenad and Savić, Jasna and Hristov, Nikola",
year = "2016",
abstract = "The deficiency of zinc (Zn) and iron (Fe) is a global issue causing not only considerable yield losses of food crops but also serious health problems. We have analysed Zn and Fe concentrations in the grains of two bread wheat cultivars along native gradient of micronutrient availability throughout Serbia. Although only 13% of the soil samples were Zn deficient and none was Fe deficient, the levels of these micronutrients in grain were rather low (median values of 21 mg kg(-1) for Zn and 36 mg kg(-1) for Fe), and even less adequate in white flour. Moreover, excessive P fertilization of calcareous soils in the major wheat growing areas strongly correlated with lower grain concentration of Zn. Our results imply that a latent Zn deficiency in wheat grain poses a high risk for grain quality relevant to human health in Serbia, where wheat bread is a staple food. k.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition",
pages = "148-141",
volume = "553",
doi = "10.1016/j.scitotenv.2016.02.102"
}

Nikolic, M., Nikolić, N., Kostić Kravljanac, L., Pavlović, J., Bosnić, P., Stević, N., Savić, J.,& Hristov, N.. (2016). The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition. in Science of the Total Environment
Elsevier, Amsterdam., 553, 141-148.
https://doi.org/10.1016/j.scitotenv.2016.02.102

Nikolic M, Nikolić N, Kostić Kravljanac L, Pavlović J, Bosnić P, Stević N, Savić J, Hristov N. The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition. in Science of the Total Environment. 2016;553:141-148.
doi:10.1016/j.scitotenv.2016.02.102 .

Nikolic, Miroslav, Nikolić, Nina, Kostić Kravljanac, Ljiljana, Pavlović, Jelena, Bosnić, Predrag, Stević, Nenad, Savić, Jasna, Hristov, Nikola, "The assessment of soil availability and wheat grain status of zinc and iron in Serbia: Implications for human nutrition" in Science of the Total Environment, 553 (2016):141-148,
https://doi.org/10.1016/j.scitotenv.2016.02.102 . .

TY  - JOUR
AU  - Pavlović, Jelena
AU  - Samardžić, Jelena T.
AU  - Kostić Kravljanac, Ljiljana
AU  - Laursen, Kristian H.
AU  - Natic, Maja
AU  - Timotijević, Gordana
AU  - Schjoerring, Jan K.
AU  - Nikolic, Miroslav
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1002
AB  - Background and Aims Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe deficiency. Previous work has shown that silicon (Si) can alleviate Fe deficiency by enhancing acquisition and root to shoot translocation of Fe. Here the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe-limiting conditions was investigated. Methods Iron (Fe-57 or naturally occurring isotopes) was measured in leaves at different positions on plants hydroponically growing with or without Si supply. In parallel, the concentration of the Fe chelator nicotianamine (NA) along with the expression of nicotianamine synthase (NAS) involved in its biosynthesis and the expression of yellow stripe-like (YSL) transcripts mediating Fe-NA transport were also determined. Key Results In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. In contrast, Si-treated plants showed an almost even Fe distribution among leaves with four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This Si-stimulated Fe export was paralleled by an increased NA accumulation and expression of the YSL1 transporter for phloem loading/unloading of the Fe-NA complex. Conclusions The results suggest that Si enhances remobilization of Fe from older to younger leaves by a more efficient NA-mediated Fe transport via the phloem. In addition, from this and previous work, a model is proposed of how Si acts to improve Fe homeostasis under Fe deficiency in cucumber.
PB  - Oxford Univ Press, Oxford
T2  - Annals of Botany
T1  - Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions
EP  - 280
IS  - 2
SP  - 271
VL  - 118
DO  - 10.1093/aob/mcw105
ER  - 

@article{
author = "Pavlović, Jelena and Samardžić, Jelena T. and Kostić Kravljanac, Ljiljana and Laursen, Kristian H. and Natic, Maja and Timotijević, Gordana and Schjoerring, Jan K. and Nikolic, Miroslav",
year = "2016",
abstract = "Background and Aims Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe deficiency. Previous work has shown that silicon (Si) can alleviate Fe deficiency by enhancing acquisition and root to shoot translocation of Fe. Here the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe-limiting conditions was investigated. Methods Iron (Fe-57 or naturally occurring isotopes) was measured in leaves at different positions on plants hydroponically growing with or without Si supply. In parallel, the concentration of the Fe chelator nicotianamine (NA) along with the expression of nicotianamine synthase (NAS) involved in its biosynthesis and the expression of yellow stripe-like (YSL) transcripts mediating Fe-NA transport were also determined. Key Results In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. In contrast, Si-treated plants showed an almost even Fe distribution among leaves with four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This Si-stimulated Fe export was paralleled by an increased NA accumulation and expression of the YSL1 transporter for phloem loading/unloading of the Fe-NA complex. Conclusions The results suggest that Si enhances remobilization of Fe from older to younger leaves by a more efficient NA-mediated Fe transport via the phloem. In addition, from this and previous work, a model is proposed of how Si acts to improve Fe homeostasis under Fe deficiency in cucumber.",
publisher = "Oxford Univ Press, Oxford",
journal = "Annals of Botany",
title = "Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions",
pages = "280-271",
number = "2",
volume = "118",
doi = "10.1093/aob/mcw105"
}

Pavlović, J., Samardžić, J. T., Kostić Kravljanac, L., Laursen, K. H., Natic, M., Timotijević, G., Schjoerring, J. K.,& Nikolic, M.. (2016). Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions. in Annals of Botany
Oxford Univ Press, Oxford., 118(2), 271-280.
https://doi.org/10.1093/aob/mcw105

Pavlović J, Samardžić JT, Kostić Kravljanac L, Laursen KH, Natic M, Timotijević G, Schjoerring JK, Nikolic M. Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions. in Annals of Botany. 2016;118(2):271-280.
doi:10.1093/aob/mcw105 .

Pavlović, Jelena, Samardžić, Jelena T., Kostić Kravljanac, Ljiljana, Laursen, Kristian H., Natic, Maja, Timotijević, Gordana, Schjoerring, Jan K., Nikolic, Miroslav, "Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions" in Annals of Botany, 118, no. 2 (2016):271-280,
https://doi.org/10.1093/aob/mcw105 . .

TY  - JOUR
AU  - Sikiric, Biljana
AU  - Stajkovic-Srbinović, O.
AU  - Cakmak, Dragan
AU  - Delic, D.
AU  - Koković, N.
AU  - Kostić Kravljanac, Ljiljana
AU  - Mrvić, Vesna
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/849
AB  - The study evaluates the effect of liming materials application in combination with NPK fertilizer and borax on macronutrient contents (nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg)), in an extremely acid soil and raspberry leaves and fruits during a two-year period. Liming increased soil pH, N mineral content, P, Ca and Mg soil content, while K content either increased (dolomite and borax application), or decreased (lime application). The N and P contents in raspberry leaves after liming increased significantly, but P content remained below the optimal values. Some treatments with lime caused a decrease in K content in leaves, while dolomite and borax application increased K content. Initially optimal Ca content in leaves increased significantly in the treatments with lime, but decreased after dolomite application. The Mg content in leaves increased after dolomite and borax application, but mainly remained below optimal values. Liming either did not alter or only slightly altered macronutrient contents in raspberry fruits.
PB  - Czech Academy Agricultural Sciences, Prague
T2  - Plant Soil and Environment
T1  - Macronutrient contents in the leaves and fruits of red raspberry as affected by liming in an extremely acid soil
EP  - 28
IS  - 1
SP  - 23
VL  - 61
DO  - 10.17221/756/2014-PSE
ER  - 

@article{
author = "Sikiric, Biljana and Stajkovic-Srbinović, O. and Cakmak, Dragan and Delic, D. and Koković, N. and Kostić Kravljanac, Ljiljana and Mrvić, Vesna",
year = "2015",
abstract = "The study evaluates the effect of liming materials application in combination with NPK fertilizer and borax on macronutrient contents (nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg)), in an extremely acid soil and raspberry leaves and fruits during a two-year period. Liming increased soil pH, N mineral content, P, Ca and Mg soil content, while K content either increased (dolomite and borax application), or decreased (lime application). The N and P contents in raspberry leaves after liming increased significantly, but P content remained below the optimal values. Some treatments with lime caused a decrease in K content in leaves, while dolomite and borax application increased K content. Initially optimal Ca content in leaves increased significantly in the treatments with lime, but decreased after dolomite application. The Mg content in leaves increased after dolomite and borax application, but mainly remained below optimal values. Liming either did not alter or only slightly altered macronutrient contents in raspberry fruits.",
publisher = "Czech Academy Agricultural Sciences, Prague",
journal = "Plant Soil and Environment",
title = "Macronutrient contents in the leaves and fruits of red raspberry as affected by liming in an extremely acid soil",
pages = "28-23",
number = "1",
volume = "61",
doi = "10.17221/756/2014-PSE"
}

Sikiric, B., Stajkovic-Srbinović, O., Cakmak, D., Delic, D., Koković, N., Kostić Kravljanac, L.,& Mrvić, V.. (2015). Macronutrient contents in the leaves and fruits of red raspberry as affected by liming in an extremely acid soil. in Plant Soil and Environment
Czech Academy Agricultural Sciences, Prague., 61(1), 23-28.
https://doi.org/10.17221/756/2014-PSE

Sikiric B, Stajkovic-Srbinović O, Cakmak D, Delic D, Koković N, Kostić Kravljanac L, Mrvić V. Macronutrient contents in the leaves and fruits of red raspberry as affected by liming in an extremely acid soil. in Plant Soil and Environment. 2015;61(1):23-28.
doi:10.17221/756/2014-PSE .

Sikiric, Biljana, Stajkovic-Srbinović, O., Cakmak, Dragan, Delic, D., Koković, N., Kostić Kravljanac, Ljiljana, Mrvić, Vesna, "Macronutrient contents in the leaves and fruits of red raspberry as affected by liming in an extremely acid soil" in Plant Soil and Environment, 61, no. 1 (2015):23-28,
https://doi.org/10.17221/756/2014-PSE . .

TY  - JOUR
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolić, Nina
AU  - Samardžić, Jelena T.
AU  - Milisavljević, Mira
AU  - Maksimović, Vuk
AU  - Cakmak, Dragan
AU  - Manojlović, Dragan
AU  - Nikolic, Miroslav
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/838
AB  - We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).
PB  - Springer, New York
T2  - Biology and Fertility of Soils
T1  - Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat
EP  - 298
IS  - 3
SP  - 289
VL  - 51
DO  - 10.1007/s00374-014-0975-y
ER  - 

@article{
author = "Kostić Kravljanac, Ljiljana and Nikolić, Nina and Samardžić, Jelena T. and Milisavljević, Mira and Maksimović, Vuk and Cakmak, Dragan and Manojlović, Dragan and Nikolic, Miroslav",
year = "2015",
abstract = "We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).",
publisher = "Springer, New York",
journal = "Biology and Fertility of Soils",
title = "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat",
pages = "298-289",
number = "3",
volume = "51",
doi = "10.1007/s00374-014-0975-y"
}

Kostić Kravljanac, L., Nikolić, N., Samardžić, J. T., Milisavljević, M., Maksimović, V., Cakmak, D., Manojlović, D.,& Nikolic, M.. (2015). Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat. in Biology and Fertility of Soils
Springer, New York., 51(3), 289-298.
https://doi.org/10.1007/s00374-014-0975-y

Kostić Kravljanac L, Nikolić N, Samardžić JT, Milisavljević M, Maksimović V, Cakmak D, Manojlović D, Nikolic M. Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat. in Biology and Fertility of Soils. 2015;51(3):289-298.
doi:10.1007/s00374-014-0975-y .

Kostić Kravljanac, Ljiljana, Nikolić, Nina, Samardžić, Jelena T., Milisavljević, Mira, Maksimović, Vuk, Cakmak, Dragan, Manojlović, Dragan, Nikolic, Miroslav, "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat" in Biology and Fertility of Soils, 51, no. 3 (2015):289-298,
https://doi.org/10.1007/s00374-014-0975-y . .

TY  - JOUR
AU  - Nikolić, Nina
AU  - Boecker, Reinhard
AU  - Kostić Kravljanac, Ljiljana
AU  - Nikolic, Miroslav
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/766
AB  - Questions: Effects of soil on vegetation patterns are commonly obscured by other environmental factors; clear and general relationships are difficult to find. How would community assembly processes be affected by a substantial change in soil characteristics when all other relevant factors are held constant? In particular, can we identify some functional adaptations which would underpin such soil-induced vegetation response? Location: Eastern Serbia: fields partially damaged by long-term and large-scale fluvial deposition of sulphidic waste from a Cu mine; subcontinental/submediterranean climate. Methods: We analysed the multivariate response of cereal weed assemblages (including biomass and foliar analyses) to a strong man-made soil gradient (from highly calcareous to highly acidic, nutrient-poor soils) over short distances (field scale). Results: The soil gradient favoured a substitution of calcicoles by calcifuges, and an increase in abundance of pseudometallophytes, with preferences for Atlantic climate, broad geographical distribution, hemicryptophytic life form, adapted to low-nutrient and acidic soils, with lower concentrations of Ca, and very narrow range of Cu concentrations in leaves. The trends of abundance of the different ecological groups of indicator species along the soil gradient were systematically reflected in the maintenance of leaf P concentrations, and strong homeostasis in biomass N:P ratio. Conclusion: Using annual weed vegetation at the field scale as a fairly simple model, we demonstrated links between gradients in soil properties (pH, nutrient availability) and floristic composition that are normally encountered over large geographic distances. We showed that leaf nutrient status, in particular the maintenance of leaf P concentrations and strong homeostasis of biomass N:P ratio, underpinned a clear functional response of vegetation to mineral stress. These findings can help to understand assembly processes leading to unusual, novel combinations of species which are typically observed as a consequence of strong environmental filtering, as for instance on sites affected by industrial activities.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Assembly Processes under Severe Abiotic Filtering: Adaptation Mechanisms of Weed Vegetation to the Gradient of Soil Constraints
IS  - 12
VL  - 9
DO  - 10.1371/journal.pone.0114290
ER  - 

@article{
author = "Nikolić, Nina and Boecker, Reinhard and Kostić Kravljanac, Ljiljana and Nikolic, Miroslav",
year = "2014",
abstract = "Questions: Effects of soil on vegetation patterns are commonly obscured by other environmental factors; clear and general relationships are difficult to find. How would community assembly processes be affected by a substantial change in soil characteristics when all other relevant factors are held constant? In particular, can we identify some functional adaptations which would underpin such soil-induced vegetation response? Location: Eastern Serbia: fields partially damaged by long-term and large-scale fluvial deposition of sulphidic waste from a Cu mine; subcontinental/submediterranean climate. Methods: We analysed the multivariate response of cereal weed assemblages (including biomass and foliar analyses) to a strong man-made soil gradient (from highly calcareous to highly acidic, nutrient-poor soils) over short distances (field scale). Results: The soil gradient favoured a substitution of calcicoles by calcifuges, and an increase in abundance of pseudometallophytes, with preferences for Atlantic climate, broad geographical distribution, hemicryptophytic life form, adapted to low-nutrient and acidic soils, with lower concentrations of Ca, and very narrow range of Cu concentrations in leaves. The trends of abundance of the different ecological groups of indicator species along the soil gradient were systematically reflected in the maintenance of leaf P concentrations, and strong homeostasis in biomass N:P ratio. Conclusion: Using annual weed vegetation at the field scale as a fairly simple model, we demonstrated links between gradients in soil properties (pH, nutrient availability) and floristic composition that are normally encountered over large geographic distances. We showed that leaf nutrient status, in particular the maintenance of leaf P concentrations and strong homeostasis of biomass N:P ratio, underpinned a clear functional response of vegetation to mineral stress. These findings can help to understand assembly processes leading to unusual, novel combinations of species which are typically observed as a consequence of strong environmental filtering, as for instance on sites affected by industrial activities.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Assembly Processes under Severe Abiotic Filtering: Adaptation Mechanisms of Weed Vegetation to the Gradient of Soil Constraints",
number = "12",
volume = "9",
doi = "10.1371/journal.pone.0114290"
}

Nikolić, N., Boecker, R., Kostić Kravljanac, L.,& Nikolic, M.. (2014). Assembly Processes under Severe Abiotic Filtering: Adaptation Mechanisms of Weed Vegetation to the Gradient of Soil Constraints. in PLoS One
Public Library Science, San Francisco., 9(12).
https://doi.org/10.1371/journal.pone.0114290

Nikolić N, Boecker R, Kostić Kravljanac L, Nikolic M. Assembly Processes under Severe Abiotic Filtering: Adaptation Mechanisms of Weed Vegetation to the Gradient of Soil Constraints. in PLoS One. 2014;9(12).
doi:10.1371/journal.pone.0114290 .

Nikolić, Nina, Boecker, Reinhard, Kostić Kravljanac, Ljiljana, Nikolic, Miroslav, "Assembly Processes under Severe Abiotic Filtering: Adaptation Mechanisms of Weed Vegetation to the Gradient of Soil Constraints" in PLoS One, 9, no. 12 (2014),
https://doi.org/10.1371/journal.pone.0114290 . .

TY  - JOUR
AU  - Mrvić, Vesna
AU  - Kostić, Ljiljana
AU  - Sikirić, Biljana
AU  - Delic, Dusica
AU  - Jaramaz, Darko
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2551
AB  - У раду су приказане различите методе одређивања границе природ ног садржаја Cr и Cd у земљишту Моравичког округа. Дистрибуција садр жаја испитиваних елемената је десно асиметрична, са високом дисперзијом, 
посебно садржаја Cr. Применом графичких метода (кумулативна крива–CDF 
и boxplot) добијене су границе природног садржаја за Cd 1,40 mg kg-1, и за Cr 
око 230 mg kg-1. За емпиријске методе коришћени су природни подаци и ло гаритамски трансформисани, при чему су добијене знатно веће вредности 
него у природној симулацији. Граничне вредности добијене различитим ме тодама се разликују. Карте показују да највећи део територије има релативно 
ниске концентрације испитиваних елемената чије границе природног садр жаја највише одговарају вредностима израчунатим методом [Median±2MAD] 
и ниже. На деловима територије са повећаним садржајима погодније су ра чунске методе [Mean±2Sd] и boxplot–обрачун горњег прага. Дате су границе 
природног садржаја за поједине хомогене геохемијске целине.
PB  - Šumarski fakultet, Univerzitet u Beogradu
T2  - Bulletin of the Faculty of Forestry
T1  - Methods for the assessment of background limits of Cd and Cr in the soil of Moravicki district
T1  - Методе за оцену граница природног садржаја Cr и Cd у земљишту Моравичког округа
EP  - 148
SP  - 137
VL  - 109
DO  - 10.2298/gsf1409137m
ER  - 

@article{
author = "Mrvić, Vesna and Kostić, Ljiljana and Sikirić, Biljana and Delic, Dusica and Jaramaz, Darko",
year = "2014",
abstract = "У раду су приказане различите методе одређивања границе природ ног садржаја Cr и Cd у земљишту Моравичког округа. Дистрибуција садр жаја испитиваних елемената је десно асиметрична, са високом дисперзијом, 
посебно садржаја Cr. Применом графичких метода (кумулативна крива–CDF 
и boxplot) добијене су границе природног садржаја за Cd 1,40 mg kg-1, и за Cr 
око 230 mg kg-1. За емпиријске методе коришћени су природни подаци и ло гаритамски трансформисани, при чему су добијене знатно веће вредности 
него у природној симулацији. Граничне вредности добијене различитим ме тодама се разликују. Карте показују да највећи део територије има релативно 
ниске концентрације испитиваних елемената чије границе природног садр жаја највише одговарају вредностима израчунатим методом [Median±2MAD] 
и ниже. На деловима територије са повећаним садржајима погодније су ра чунске методе [Mean±2Sd] и boxplot–обрачун горњег прага. Дате су границе 
природног садржаја за поједине хомогене геохемијске целине.",
publisher = "Šumarski fakultet, Univerzitet u Beogradu",
journal = "Bulletin of the Faculty of Forestry",
title = "Methods for the assessment of background limits of Cd and Cr in the soil of Moravicki district, Методе за оцену граница природног садржаја Cr и Cd у земљишту Моравичког округа",
pages = "148-137",
volume = "109",
doi = "10.2298/gsf1409137m"
}

Mrvić, V., Kostić, L., Sikirić, B., Delic, D.,& Jaramaz, D.. (2014). Methods for the assessment of background limits of Cd and Cr in the soil of Moravicki district. in Bulletin of the Faculty of Forestry
Šumarski fakultet, Univerzitet u Beogradu., 109, 137-148.
https://doi.org/10.2298/gsf1409137m

Mrvić V, Kostić L, Sikirić B, Delic D, Jaramaz D. Methods for the assessment of background limits of Cd and Cr in the soil of Moravicki district. in Bulletin of the Faculty of Forestry. 2014;109:137-148.
doi:10.2298/gsf1409137m .

Mrvić, Vesna, Kostić, Ljiljana, Sikirić, Biljana, Delic, Dusica, Jaramaz, Darko, "Methods for the assessment of background limits of Cd and Cr in the soil of Moravicki district" in Bulletin of the Faculty of Forestry, 109 (2014):137-148,
https://doi.org/10.2298/gsf1409137m . .

TY  - CONF
AU  - Bosnić, Predrag
AU  - Savic, Jasna
AU  - Kostić, Ljiljana
AU  - Stevic, Nenad
AU  - Pavlović, Jelena
AU  - Lazic, Marina
AU  - Marjanovic Jeromela, Ana
AU  - Hristov, Nikola
AU  - Nikolić, Nina
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2504
AB  - Zinc (Zn) is an essential microelement for plants and also an important nutritional and health factor in humans. As a consequence of low Zn availability in soils, nearly half of the world's population suffers from Zn malnutrition. Zn deficiency has serious implications for human health (e.g. impairments in physical development, immune system, brain function and learning ability) and thus for the overall economy of a country; it is most severe in nations who depend on cereals as the main staple food. A critical Zn concentration in the whole grain for humans that depend on cereal based diets is 24 mg kg-1 dry matter. Research focused on increase of Zn content in cereals (biofortification), is the strategic priority in many countries. In Serbia however, the awareness of this problem is lacking, and no systematic survey of Zn availability in soi ls and concentrations
 in cereal grains has been undertaken so far.
Our study included 156 grain samples of the two major bread wheat varieties (Simonida and NS 40S) collected at 89 localities throughout Serbia. We analyzed soil pH, available Zn and grain Zn concentration together by principle component analysis and multiple linear regression. Wheat varieties did not differ in ability to accumulate Zn in grains. Both soil pH and available Zn concentration were the nominally significant predictors for grain Zn concentration and explained about 12 and 9% of the encountered variation, respectively. Zn concentration below the critical limit (24 mg kg-1) was found in 58% of grain samples (values in the range 11-61 mg kg-1, median only 21.3  mg kg-1), while in only 14% of soil samples the available Zn was below the critical value (05 mg kg-1).
The most severe lack of Zn in grains (below 18 mg kg-1) was observed in samples from the major production regions of bread wheat (e.g. Pancevo, Vrbas and Sremska Mitrovica). The alarming results of this survey indicate that Serbia urgently needs a strategy for Zn biofortification, primarily through a breeding program to enhance Zn efficiency as a sustainable alternative to application of Zn fertilizers.
PB  - Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade
C3  - 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
T1  - Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2504
ER  - 

@conference{
author = "Bosnić, Predrag and Savic, Jasna and Kostić, Ljiljana and Stevic, Nenad and Pavlović, Jelena and Lazic, Marina and Marjanovic Jeromela, Ana and Hristov, Nikola and Nikolić, Nina and Nikolic, Miroslav",
year = "2013",
abstract = "Zinc (Zn) is an essential microelement for plants and also an important nutritional and health factor in humans. As a consequence of low Zn availability in soils, nearly half of the world's population suffers from Zn malnutrition. Zn deficiency has serious implications for human health (e.g. impairments in physical development, immune system, brain function and learning ability) and thus for the overall economy of a country; it is most severe in nations who depend on cereals as the main staple food. A critical Zn concentration in the whole grain for humans that depend on cereal based diets is 24 mg kg-1 dry matter. Research focused on increase of Zn content in cereals (biofortification), is the strategic priority in many countries. In Serbia however, the awareness of this problem is lacking, and no systematic survey of Zn availability in soi ls and concentrations
 in cereal grains has been undertaken so far.
Our study included 156 grain samples of the two major bread wheat varieties (Simonida and NS 40S) collected at 89 localities throughout Serbia. We analyzed soil pH, available Zn and grain Zn concentration together by principle component analysis and multiple linear regression. Wheat varieties did not differ in ability to accumulate Zn in grains. Both soil pH and available Zn concentration were the nominally significant predictors for grain Zn concentration and explained about 12 and 9% of the encountered variation, respectively. Zn concentration below the critical limit (24 mg kg-1) was found in 58% of grain samples (values in the range 11-61 mg kg-1, median only 21.3  mg kg-1), while in only 14% of soil samples the available Zn was below the critical value (05 mg kg-1).
The most severe lack of Zn in grains (below 18 mg kg-1) was observed in samples from the major production regions of bread wheat (e.g. Pancevo, Vrbas and Sremska Mitrovica). The alarming results of this survey indicate that Serbia urgently needs a strategy for Zn biofortification, primarily through a breeding program to enhance Zn efficiency as a sustainable alternative to application of Zn fertilizers.",
publisher = "Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade",
journal = "1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia",
title = "Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2504"
}

Bosnić, P., Savic, J., Kostić, L., Stevic, N., Pavlović, J., Lazic, M., Marjanovic Jeromela, A., Hristov, N., Nikolić, N.,& Nikolic, M.. (2013). Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia
Serbian Plant Physiology Society; Institute for Biological Research “Siniša Stanković”, University of Belgrade; Faculty of Biology, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2504

Bosnić P, Savic J, Kostić L, Stevic N, Pavlović J, Lazic M, Marjanovic Jeromela A, Hristov N, Nikolić N, Nikolic M. Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia. in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia. 2013;.
https://hdl.handle.net/21.15107/rcub_rimsi_2504 .

Bosnić, Predrag, Savic, Jasna, Kostić, Ljiljana, Stevic, Nenad, Pavlović, Jelena, Lazic, Marina, Marjanovic Jeromela, Ana, Hristov, Nikola, Nikolić, Nina, Nikolic, Miroslav, "Zn concentrations in wheat grains along the gradient of native Zn soil availability in Serbia" in 1st International Conference on Plant Biology - Programme and Abstracts, June 4-7, 2013, Subotica, Serbia (2013),
https://hdl.handle.net/21.15107/rcub_rimsi_2504 .

TY  - CONF
AU  - Kostić, Ljiljana
AU  - Samardzic, Jelena
AU  - Nikolić, Nina
AU  - Liang, Yongchao
AU  - Nikolic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2488
AB  - Pollution from industrial activities is increasingly creating marginal conditions for crop production worldwide. Nutrient deficiency, and in particular phosphorus (P) deficiency is an often overlooked factor which can be a severe constraint for plant growth in soils affected my mining activates (Nikolic et al., 2011). The root exudation of carboxylates (mainly citrate and malate) has been considered as the major root response to mobilize sparingly soluble P in the rhizosphere. Therefore the enhanced activity of phosphoenolpyruvate carboxylase (PEPC) appears to be a key P-independent metabolic bypass reaction of malate/citrate biosynthesis in P deficient plants. Although the beneficial role of silicon (Si) on plant growth has been reported on several plant species grown under P deficient conditions the underlying mechanism is still unknown. 
The pot experiments were conducted with winter wheat, including Si fertilizers in addition to the conventional reclamation amendments (e.g. cow manure, NPK and lime) in the acid soil polluted by sulphidic mine tailings (collected form the Timok floodplain). The main focus of this study was on the dynamics of P pools in wheat rhizosphere, and on the molecular mechanism of root response to P availability in the rhizosphere (expression of TaPT1 and TaPT2, encoding Pi transporters, TaPEPC encoding PEPC, and MATE-family genes encoding Al-activated citrate efflux transporter). All the amendments induced a significant change in the rhizosphere P fractions (readily available, Al- and Fe-bound P). For instance, Si supply has caused significant increase in readily available P, and reduction in Al-P and Fe-P. This can be attributed to synergetic effect of pH increase and reduction of P sorption by Al- and Fe-oxides. The leaf P concentration in wheat plants treated with Si significantly increased and was in the range of leaf P concentration in P-fertilized plants. While the expressions of the root TaPT2 and TaPEPC were down-regulated by P availability in the rhizosphere and plant P status, TaPT1 and TaMATE showed different pattern with markedly enhanced expression at Si treatment irrespectively of the P supply. In conclusion, Si nutrition effectively alleviates P deficiency in wheat by 1) increased P availability in rhizosphere, most probably due to MATE-mediated citrate exudation, and 2) enhanced P acquisition as a consequence of Si-promoted expressions of PT1 transporter in root plasma membrane.
PB  - Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade
C3  - 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, June 4-7, Subotica, Serbia. Abstracts, p. 48
T1  - The mechanisms of Si-mediated alleviation of P deficiency in wheat grown in acid soils polluted by mine tailings
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2488
ER  - 

@conference{
author = "Kostić, Ljiljana and Samardzic, Jelena and Nikolić, Nina and Liang, Yongchao and Nikolic, Miroslav",
year = "2013",
abstract = "Pollution from industrial activities is increasingly creating marginal conditions for crop production worldwide. Nutrient deficiency, and in particular phosphorus (P) deficiency is an often overlooked factor which can be a severe constraint for plant growth in soils affected my mining activates (Nikolic et al., 2011). The root exudation of carboxylates (mainly citrate and malate) has been considered as the major root response to mobilize sparingly soluble P in the rhizosphere. Therefore the enhanced activity of phosphoenolpyruvate carboxylase (PEPC) appears to be a key P-independent metabolic bypass reaction of malate/citrate biosynthesis in P deficient plants. Although the beneficial role of silicon (Si) on plant growth has been reported on several plant species grown under P deficient conditions the underlying mechanism is still unknown. 
The pot experiments were conducted with winter wheat, including Si fertilizers in addition to the conventional reclamation amendments (e.g. cow manure, NPK and lime) in the acid soil polluted by sulphidic mine tailings (collected form the Timok floodplain). The main focus of this study was on the dynamics of P pools in wheat rhizosphere, and on the molecular mechanism of root response to P availability in the rhizosphere (expression of TaPT1 and TaPT2, encoding Pi transporters, TaPEPC encoding PEPC, and MATE-family genes encoding Al-activated citrate efflux transporter). All the amendments induced a significant change in the rhizosphere P fractions (readily available, Al- and Fe-bound P). For instance, Si supply has caused significant increase in readily available P, and reduction in Al-P and Fe-P. This can be attributed to synergetic effect of pH increase and reduction of P sorption by Al- and Fe-oxides. The leaf P concentration in wheat plants treated with Si significantly increased and was in the range of leaf P concentration in P-fertilized plants. While the expressions of the root TaPT2 and TaPEPC were down-regulated by P availability in the rhizosphere and plant P status, TaPT1 and TaMATE showed different pattern with markedly enhanced expression at Si treatment irrespectively of the P supply. In conclusion, Si nutrition effectively alleviates P deficiency in wheat by 1) increased P availability in rhizosphere, most probably due to MATE-mediated citrate exudation, and 2) enhanced P acquisition as a consequence of Si-promoted expressions of PT1 transporter in root plasma membrane.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade",
journal = "1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, June 4-7, Subotica, Serbia. Abstracts, p. 48",
title = "The mechanisms of Si-mediated alleviation of P deficiency in wheat grown in acid soils polluted by mine tailings",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2488"
}

Kostić, L., Samardzic, J., Nikolić, N., Liang, Y.,& Nikolic, M.. (2013). The mechanisms of Si-mediated alleviation of P deficiency in wheat grown in acid soils polluted by mine tailings. in 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, June 4-7, Subotica, Serbia. Abstracts, p. 48
Serbian Plant Physiology Society, Institute for Biological Research ,,Sinisa Stankovic", University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimsi_2488

Kostić L, Samardzic J, Nikolić N, Liang Y, Nikolic M. The mechanisms of Si-mediated alleviation of P deficiency in wheat grown in acid soils polluted by mine tailings. in 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, June 4-7, Subotica, Serbia. Abstracts, p. 48. 2013;.
https://hdl.handle.net/21.15107/rcub_rimsi_2488 .

Kostić, Ljiljana, Samardzic, Jelena, Nikolić, Nina, Liang, Yongchao, Nikolic, Miroslav, "The mechanisms of Si-mediated alleviation of P deficiency in wheat grown in acid soils polluted by mine tailings" in 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society, June 4-7, Subotica, Serbia. Abstracts, p. 48 (2013),
https://hdl.handle.net/21.15107/rcub_rimsi_2488 .

TY  - CONF
AU  - Pavlović, Jelena
AU  - Samardzic, Jelena
AU  - Ilic, Petar
AU  - Maksimović, Vuk
AU  - Kostić, Ljiljana
AU  - Stevic, Nenad
AU  - Nikolić, Nina
AU  - Liang, Yongchao
AU  - Nikolic, Miroslav
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2450
AB  - Silicon (Si) and iron (Fe) are respectively the second and the forth most abundant
minerals in the earth’s crust. While the essentiality of Fe is discovered at the middle
of the 19th century, Si is still not fully accepted as an essential element for higher
plants. However, Si is the only known element that alleviates multiple stresses in
plants (e.g. metal excess, drought, salt, lodging, diseases and pests). Fe deficiency
chlorosis is a wide-spread nutritional disorder of many crops grown in calcareous and
alkaline soils. The various adaptation mechanisms are involved in Fe acquisition from
rhizosphere by roots of the so-called strategy 1 plants (all dicots and monocot species,
with exception of grasses which belong to strategy 2), i.e. morphological changes (e.g.
lateral roots and enhanced root hair formation in the apical zones) and physiological
changes such as enhanced proton excretion, FeIII reduction by a plasma membrane
reductase and Fe uptake via an inducible FeII transporter (IRT1). These root responses
have been studied and characterized mainly in the nutrient solutions without Si
supply. Therefore, unambiguous information on an interaction between these two
mineral elements is still lacking. We demonstrated for the first time that the
application of Si in nutrient solution experiments also ameliorates Fe deficiency
chlorosis in cucumber, a Si accumulating dicot, which is also commonly used as a
model plant of strategy 1. I will present recent work from our lab in the context of the
effect of Si on both physiological (e.g. FeIII reducing capacity, release of phenolics
and organic acids) and molecular (e.g. expression of FRO2, HA1 and IRT1, the genes
coding FeIII chelate reductase, H+-ATPase and IRT1, respectively) aspects of root
responses to Fe deficiency. In particular, I will focus on the storage and utilization of root apoplastic Fe, root-to-shoot Fe transport and utilization of Fe from the leaf
apoplast. Based on these investigations we propose the possible role of Si in Fe
deficiency stress as 1) increasing apoplastic Fe pool in roots and 2) improving internal
Fe status and thus delaying Fe chlorosis, rather than a direct regulatory/signaling
effect of Si on the key Fe deficiency inducible root responses.
PB  - ISSAG
C3  - Proceedings of The 5th International Conference on Silicon in Agriculture, September 13-18, 2011, Beijing, China
T1  - Silicon ameliorates iron deficiency chlorosis in strategy I plants: first evidence and possible mechanism (s)
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2450
ER  - 

@conference{
author = "Pavlović, Jelena and Samardzic, Jelena and Ilic, Petar and Maksimović, Vuk and Kostić, Ljiljana and Stevic, Nenad and Nikolić, Nina and Liang, Yongchao and Nikolic, Miroslav",
year = "2011",
abstract = "Silicon (Si) and iron (Fe) are respectively the second and the forth most abundant
minerals in the earth’s crust. While the essentiality of Fe is discovered at the middle
of the 19th century, Si is still not fully accepted as an essential element for higher
plants. However, Si is the only known element that alleviates multiple stresses in
plants (e.g. metal excess, drought, salt, lodging, diseases and pests). Fe deficiency
chlorosis is a wide-spread nutritional disorder of many crops grown in calcareous and
alkaline soils. The various adaptation mechanisms are involved in Fe acquisition from
rhizosphere by roots of the so-called strategy 1 plants (all dicots and monocot species,
with exception of grasses which belong to strategy 2), i.e. morphological changes (e.g.
lateral roots and enhanced root hair formation in the apical zones) and physiological
changes such as enhanced proton excretion, FeIII reduction by a plasma membrane
reductase and Fe uptake via an inducible FeII transporter (IRT1). These root responses
have been studied and characterized mainly in the nutrient solutions without Si
supply. Therefore, unambiguous information on an interaction between these two
mineral elements is still lacking. We demonstrated for the first time that the
application of Si in nutrient solution experiments also ameliorates Fe deficiency
chlorosis in cucumber, a Si accumulating dicot, which is also commonly used as a
model plant of strategy 1. I will present recent work from our lab in the context of the
effect of Si on both physiological (e.g. FeIII reducing capacity, release of phenolics
and organic acids) and molecular (e.g. expression of FRO2, HA1 and IRT1, the genes
coding FeIII chelate reductase, H+-ATPase and IRT1, respectively) aspects of root
responses to Fe deficiency. In particular, I will focus on the storage and utilization of root apoplastic Fe, root-to-shoot Fe transport and utilization of Fe from the leaf
apoplast. Based on these investigations we propose the possible role of Si in Fe
deficiency stress as 1) increasing apoplastic Fe pool in roots and 2) improving internal
Fe status and thus delaying Fe chlorosis, rather than a direct regulatory/signaling
effect of Si on the key Fe deficiency inducible root responses.",
publisher = "ISSAG",
journal = "Proceedings of The 5th International Conference on Silicon in Agriculture, September 13-18, 2011, Beijing, China",
title = "Silicon ameliorates iron deficiency chlorosis in strategy I plants: first evidence and possible mechanism (s)",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2450"
}

Pavlović, J., Samardzic, J., Ilic, P., Maksimović, V., Kostić, L., Stevic, N., Nikolić, N., Liang, Y.,& Nikolic, M.. (2011). Silicon ameliorates iron deficiency chlorosis in strategy I plants: first evidence and possible mechanism (s). in Proceedings of The 5th International Conference on Silicon in Agriculture, September 13-18, 2011, Beijing, China
ISSAG..
https://hdl.handle.net/21.15107/rcub_rimsi_2450

Pavlović J, Samardzic J, Ilic P, Maksimović V, Kostić L, Stevic N, Nikolić N, Liang Y, Nikolic M. Silicon ameliorates iron deficiency chlorosis in strategy I plants: first evidence and possible mechanism (s). in Proceedings of The 5th International Conference on Silicon in Agriculture, September 13-18, 2011, Beijing, China. 2011;.
https://hdl.handle.net/21.15107/rcub_rimsi_2450 .

Pavlović, Jelena, Samardzic, Jelena, Ilic, Petar, Maksimović, Vuk, Kostić, Ljiljana, Stevic, Nenad, Nikolić, Nina, Liang, Yongchao, Nikolic, Miroslav, "Silicon ameliorates iron deficiency chlorosis in strategy I plants: first evidence and possible mechanism (s)" in Proceedings of The 5th International Conference on Silicon in Agriculture, September 13-18, 2011, Beijing, China (2011),
https://hdl.handle.net/21.15107/rcub_rimsi_2450 .